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Vitamin B1/Thiamine Metabolism
Last Updated: 2018-12-22
The biosynthesis of thiamine begins with pyrithiamine reacting with thiaminase 2 resulting in the release of 4-Amino-5-hydroxymethyl-2-methylpyrimidine. The latter compound reacts with a hydroxymethylpyrimidine/phosphomethylpyrimidine kinase resulting in the release of 4-amino-2-methyl-5-phosphomethylpyrimidine. The latter compound reacts with a hydroxymethylpyrimidine/phosphomethylpyrimidine kinase resulting in the release of 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate. The latter compound reacts with 4-methyl-5-(2-phosphonooxyethyl)thiazole, a product of oxythiamine metabolism, through a Thiamine biosynthetic bifunctional enzyme resultin in the release of a Thiamine monophosphate. The latter compound is phosphatased through a acid phosphatase complex resulting in the release of thiamine. The latter compound is phosphorylated through a thiamin pyrophosphokinase resulting in the release of thiamine pyrophosphate.
Vitamin B1/Thiamine Metabolism References
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Kawasaki Y: Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway. J Bacteriol. 1993 Aug;175(16):5153-8.Pubmed: 8394314
Nosaka K, Nishimura H, Kawasaki Y, Tsujihara T, Iwashima A: Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae. J Biol Chem. 1994 Dec 2;269(48):30510-6.Pubmed: 7982968
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