Protein/105 Cytoplasm/Stoichiometry/6.0
type: Stoichiometry
physical entity: Nucleoside diphosphate kinase A
stoichiometric coefficient: 6.0

Reference/PubChem-compound 6022
type: UnificationXref
id: 6022
db: PubChem-compound

Reference/UniProt Q9HAS3
type: UnificationXref
id: Q9HAS3
db: UniProt

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Reference/ChEBI CHEBI 16134
type: UnificationXref
id: CHEBI:16134
db: ChEBI

Compound/PW C000035 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ammonia
stoichiometric coefficient: 1.0

Gemcitabine
type: SmallMoleculeReference
xref: Reference/PubChem-compound 60750
xref: Reference/ChEBI CHEBI 175901
xref: Reference/KEGG Compound C07650
xref: Reference/ChemSpider 54753
xref: Reference/CAS 95058-81-4
xref: Reference/HMDB HMDB0014584
chemical formula: C9H11F2N3O4
molecular weight: 263.07178
structure: ChemicalStructure/PW C008951

Compound/PW C000414 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Compound/PW C040476 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine Monophosphate
stoichiometric coefficient: 1.0

Compound/PW C040657 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Difluorodeoxyuridine monophosphate
stoichiometric coefficient: 1.0

Gemcitabine diphosphate
type: SmallMolecule
cellular location: Nucleus
entity reference: Gemcitabine diphosphate

Thymidylate synthase
type: Protein
cellular location: Cytoplasm
entity reference: Thymidylate synthase
name: SubPathwayInhibition

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Ammonia
type: SmallMoleculeReference
xref: Reference/BioCyc AMMONIA
xref: Reference/CAS 7664-41-7
xref: Reference/HMDB HMDB0000051
xref: Reference/PubChem-compound 222
xref: Reference/KEGG Compound C00014
xref: Reference/ChEBI CHEBI 16134
xref: Reference/ChemSpider 217
chemical formula: H3N
molecular weight: 17.026548
structure: ChemicalStructure/PW C000035

Deoxycytidine kinase
type: Complex
component stoichiometry: Protein/7 Nucleus/Stoichiometry/2.0
cellular location: Nucleus
component: Deoxycytidine kinase

Reference/HMDB HMDB0001341
type: UnificationXref
id: HMDB0001341
db: HMDB

ChemicalStructure/PW C040476
type: ChemicalStructure
structure format: SMILES

Ribonucleoside-diphosphate reductase subunit M2
type: Protein
cellular location: Cytoplasm
entity reference: Ribonucleoside-diphosphate reductase subunit M2

Ribonucleoside-diphosphate reductase 1
type: Complex
component stoichiometry: Protein/107 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/104 Cytoplasm/Stoichiometry/1.0
cellular location: Cytoplasm
component: Ribonucleoside-diphosphate reductase subunit M2
component: Ribonucleoside-diphosphate reductase large subunit
name: SubPathwayInhibition

Compound/PW C040483 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine diphosphate
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

5'(3')-deoxyribonucleotidase, cytosolic type
type: Protein
cellular location: Cytoplasm
entity reference: 5'(3')-deoxyribonucleotidase, cytosolic type

Thymidylate synthase
type: ProteinReference
xref: Reference/UniProt P04818
organism: Homo sapiens

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

Solute carrier family 28 member 3
type: ProteinReference
xref: Reference/UniProt Q9HAS3

Reference/PubChem-compound 6420157
type: UnificationXref
id: 6420157
db: PubChem-compound

Reference/ChEBI CHEBI 175901
type: UnificationXref
id: CHEBI:175901
db: ChEBI

ChemicalStructure/PW C001034
type: ChemicalStructure
structure format: SMILES

ReactionCatalysis1459
type: Catalysis
controller: Deoxycytidine kinase
controlled: 2.7.1.74
control type: ACTIVATION

Water
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Water

Reference/ChemSpider 4925716
type: UnificationXref
id: 4925716
db: ChemSpider

Protein/104 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ribonucleoside-diphosphate reductase large subunit
stoichiometric coefficient: 1.0

Adenosine diphosphate
type: SmallMolecule
cellular location: Nucleus
entity reference: Adenosine diphosphate

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

Gemcitabine Action Pathway (old)
type: Pathway
pathway order: PathwayEnzymeReaction/2998
pathway order: PathwayEnzymeReaction/3218
pathway order: PathwayEnzymeReaction/2999
pathway order: PathwaySubPathway/741
pathway order: PathwayEnzymeReaction/2997
pathway order: PathwayEnzymeReaction/3002
pathway order: PathwayTransport/2992
pathway order: PathwaySubPathway/744
pathway order: PathwayTransport/2993
pathway order: PathwaySubPathway/742
pathway order: PathwayTransport/2991
pathway order: PathwaySubPathway/743
pathway component: TransportCatalysis183
pathway component: TransportCatalysis182
pathway component: TransportCatalysis181
pathway component: ReactionCatalysis2051
pathway component: 2.7.4.6
pathway component: SubPathwayControl741
pathway component: SubPathwayControl742
pathway component: SubPathwayControl743
pathway component: ReactionCatalysis1478
pathway component: ReactionCatalysis1469
pathway component: 3.5.4.12
pathway component: ReactionCatalysis1955
pathway component: ReactionCatalysis1459
pathway component: Reaction1735
pathway component: Transport223
pathway component: 2.7.1.74
pathway component: 3.1.3.-
pathway component: Incorporation into DNA and RNA
xref: Reference/PathWhiz PW000247
xref: Reference/SMPDB SMP0000446
organism: Homo sapiens

Reference/BioCyc ADP
type: UnificationXref
id: ADP
db: BioCyc

ChemicalStructure/PW C040483
type: ChemicalStructure
structure format: SMILES

5'(3')-deoxyribonucleotidase, cytosolic type
type: Complex
component stoichiometry: Protein/6 Cytoplasm/Stoichiometry/2.0
cellular location: Cytoplasm
component: 5'(3')-deoxyribonucleotidase, cytosolic type

Reference/UniProt P27707
type: UnificationXref
id: P27707
db: UniProt

Deoxycytidylate deaminase
type: ProteinReference
xref: Reference/UniProt P32321
organism: Homo sapiens

UMP-CMP kinase
type: ProteinReference
xref: Reference/UniProt P30085
organism: Homo sapiens

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

ChemicalStructure/PW C040485
type: ChemicalStructure
structure format: SMILES

TransportCatalysis183
type: Catalysis
controller: Solute carrier family 28 member 3
controlled: Transport223
control type: ACTIVATION

TransportCatalysis182
type: Catalysis
controller: Sodium/nucleoside cotransporter 1
controlled: Transport223
control type: ACTIVATION

TransportCatalysis181
type: Catalysis
controller: Equilibrative nucleoside transporter 1
controlled: Transport223
control type: ACTIVATION

Homo sapiens
type: BioSource
xref: Reference/TAXONOMY 9606

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Compound/PW C008951 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine
stoichiometric coefficient: 1.0

Reference/KEGG Compound C07650
type: UnificationXref
id: C07650
db: KEGG Compound

ReactionCatalysis1469
type: Catalysis
controller: UMP-CMP kinase
controlled: Reaction1735
control type: ACTIVATION

ChemicalStructure/PW C008951
type: ChemicalStructure
structure format: SMILES

Reference/BioCyc AMMONIA
type: UnificationXref
id: AMMONIA
db: BioCyc

ChemicalStructure/PW C000035
type: ChemicalStructure
structure format: SMILES
structure data: N

Adenosine diphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Adenosine diphosphate

Ribonucleoside-diphosphate reductase large subunit
type: Protein
cellular location: Cytoplasm
entity reference: Ribonucleoside-diphosphate reductase large subunit

5'(3')-deoxyribonucleotidase, cytosolic type
type: ProteinReference
xref: Reference/UniProt Q8TCD5
organism: Homo sapiens

Deoxycytidine kinase
type: ProteinReference
xref: Reference/UniProt P27707
organism: Homo sapiens

Reference/PubChem-compound 60750
type: UnificationXref
id: 60750
db: PubChem-compound

Reference/UniProt O00337
type: UnificationXref
id: O00337
db: UniProt

Equilibrative nucleoside transporter 1
type: ProteinReference
xref: Reference/UniProt Q99808

Gemcitabine Monophosphate
type: SmallMoleculeReference
xref: Reference/ChemSpider 439295
xref: Reference/HMDB HMDB0061262
xref: Reference/PubChem-compound 503015
chemical formula: C9H12F2N3O7P
molecular weight: 343.0381
structure: ChemicalStructure/PW C040476

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

ChemicalStructure/PW C040657
type: ChemicalStructure
structure format: SMILES

Reference/UniProt P17812
type: UnificationXref
id: P17812
db: UniProt

Reference/ChEBI CHEBI 16761
type: UnificationXref
id: CHEBI:16761
db: ChEBI

ReactionCatalysis1478
type: Catalysis
controller: Nucleoside diphosphate kinase A
controlled: 2.7.4.6
control type: ACTIVATION

Compound/PW C040483 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine diphosphate
stoichiometric coefficient: 1.0

3.5.4.12
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000035 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040476 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040657 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
right: Ammonia
right: Difluorodeoxyuridine monophosphate
spontaneous: false
EC number: 3.5.4.12
left: Water
left: Gemcitabine Monophosphate
name: Gemcitabine Monophosphate + Water → Ammonia + Difluorodeoxyuridine monophosphate
conversion direction: LEFT_TO_RIGHT

Reference/UniProt P23921
type: UnificationXref
id: P23921
db: UniProt

Reference/ChemSpider 115568
type: UnificationXref
id: 115568
db: ChemSpider

Gemcitabine
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Gemcitabine

Ammonia
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Ammonia

Reference/PubChem-compound 503015
type: UnificationXref
id: 503015
db: PubChem-compound

Gemcitabine
type: SmallMolecule
cellular location: Nucleus
entity reference: Gemcitabine

Difluorodeoxyuridine monophosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Difluorodeoxyuridine monophosphate
name: SubPathwayInhibitor

Gemcitabine diphosphate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0060639
xref: Reference/ChemSpider 4925716
xref: Reference/PubChem-compound 6420157
chemical formula: C9H13F2N3O10P2
molecular weight: 423.00443
structure: ChemicalStructure/PW C040483

Reference/TAXONOMY 9606
type: UnificationXref
id: 9606
db: TAXONOMY

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Gemcitabine triphosphate
type: SmallMoleculeReference
xref: Reference/PubChem-compound 130659
xref: Reference/ChemSpider 115568
xref: Reference/HMDB HMDB0060638
chemical formula: C9H14F2N3O13P3
molecular weight: 502.97076
structure: ChemicalStructure/PW C040485

ReactionCatalysis2051
type: Catalysis
controller: 5'(3')-deoxyribonucleotidase, cytosolic type
controlled: 3.1.3.-
control type: ACTIVATION

Reference/SMPDB SMP0000446
type: UnificationXref
id: SMP0000446
db: SMPDB

Reference/ChemSpider 5800
type: UnificationXref
id: 5800
db: ChemSpider

2.7.4.6
type: BiochemicalReaction
participant stoichiometry: Compound/PW C040485 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040483 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
right: Gemcitabine triphosphate
right: Adenosine diphosphate
spontaneous: false
EC number: 2.7.4.6
left: Adenosine triphosphate
left: Gemcitabine diphosphate
name: Adenosine triphosphate + Gemcitabine diphosphate → Adenosine diphosphate + Gemcitabine triphosphate
conversion direction: LEFT_TO_RIGHT

SubPathwayControl741
type: Interaction
name: SubPathway741Control
name: InhibitorySubPathwayControl
participant: Thymidylate synthase
participant: Difluorodeoxyuridine monophosphate inhibition of
participant: Difluorodeoxyuridine monophosphate

SubPathwayControl742
type: Interaction
name: SubPathway742Control
name: InhibitorySubPathwayControl
participant: Ribonucleoside-diphosphate reductase 2
participant: Ribonucleoside-diphosphate reductase 1
participant: Gemcitabine triphosphate
participant: Gemcitabine Di- and Triphosphate inhibit Ribonucleoside-diphosphate reductase
participant: Gemcitabine diphosphate

SubPathwayControl743
type: Interaction
name: SubPathway743Control
name: InhibitorySubPathwayControl
participant: Gemcitabine triphosphate
participant: Gemcitabine Triphosphate inhibits CTP synthase 1
participant: CTP synthase 1

Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine diphosphate
stoichiometric coefficient: 1.0

Nucleoside diphosphate kinase A
type: ProteinReference
xref: Reference/UniProt P15531
organism: Homo sapiens

Ribonucleoside-diphosphate reductase large subunit
type: ProteinReference
xref: Reference/UniProt P23921
organism: Homo sapiens

Reference/ChemSpider 217
type: UnificationXref
id: 217
db: ChemSpider

Ribonucleoside-diphosphate reductase subunit M2
type: ProteinReference
xref: Reference/UniProt P31350
organism: Homo sapiens

Solute carrier family 28 member 3
type: Protein
entity reference: Solute carrier family 28 member 3

Protein/7 Nucleus/Stoichiometry/2.0
type: Stoichiometry
physical entity: Deoxycytidine kinase
stoichiometric coefficient: 2.0

Reference/CAS 95058-81-4
type: UnificationXref
id: 95058-81-4
db: CAS

Gemcitabine triphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Gemcitabine triphosphate
name: SubPathwayInhibitor

Reaction1735
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000414 Nucleus/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040483 Nucleus/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Nucleus/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040476 Nucleus/Stoichiometry/1.0
right: Gemcitabine diphosphate
right: Adenosine diphosphate
spontaneous: false
left: Gemcitabine Monophosphate
left: Adenosine triphosphate
name: Adenosine triphosphate + Gemcitabine Monophosphate → Adenosine diphosphate + Gemcitabine diphosphate
conversion direction: LEFT_TO_RIGHT

Reference/HMDB HMDB0000051
type: UnificationXref
id: HMDB0000051
db: HMDB

Sodium/nucleoside cotransporter 1
type: ProteinReference
xref: Reference/UniProt O00337

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

Gemcitabine triphosphate
type: SmallMolecule
cellular location: Nucleus
entity reference: Gemcitabine triphosphate
name: SubPathwayInput

2.7.1.74
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000414 Nucleus/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Nucleus/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040476 Nucleus/Stoichiometry/1.0
participant stoichiometry: Compound/PW C008951 Nucleus/Stoichiometry/1.0
right: Adenosine diphosphate
right: Gemcitabine Monophosphate
spontaneous: false
EC number: 2.7.1.74
left: Gemcitabine
left: Adenosine triphosphate
name: Adenosine triphosphate + Gemcitabine → Adenosine diphosphate + Gemcitabine Monophosphate
conversion direction: LEFT_TO_RIGHT

Reference/UniProt P30085
type: UnificationXref
id: P30085
db: UniProt

Reference/UniProt Q7LG56
type: UnificationXref
id: Q7LG56
db: UniProt

CTP synthase 1
type: Protein
cellular location: Cytoplasm
entity reference: CTP synthase 1
name: SubPathwayInhibition

Compound/PW C008951 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Reference/KEGG Compound C00008
type: UnificationXref
id: C00008
db: KEGG Compound

Reference/ChemSpider 54753
type: UnificationXref
id: 54753
db: ChemSpider

Reference/PubChem-compound 130659
type: UnificationXref
id: 130659
db: PubChem-compound

Protein/6 Cytoplasm/Stoichiometry/2.0
type: Stoichiometry
physical entity: 5'(3')-deoxyribonucleotidase, cytosolic type
stoichiometric coefficient: 2.0

Protein/107 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ribonucleoside-diphosphate reductase subunit M2
stoichiometric coefficient: 1.0

SubPathwayInteraction744
type: Interaction
name: SubPathway744Reaction
name: SubPathwayReaction
participant: Gemcitabine triphosphate

Reference/CAS 58-64-0
type: UnificationXref
id: 58-64-0
db: CAS

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Difluorodeoxyuridine monophosphate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0061204
chemical formula: C9H11F2N2O8P
molecular weight: 344.0221
structure: ChemicalStructure/PW C040657

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Compound/PW C001034 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine diphosphate
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0014584
type: UnificationXref
id: HMDB0014584
db: HMDB

Ribonucleoside-diphosphate reductase subunit M2 B
type: Protein
cellular location: Cytoplasm
entity reference: Ribonucleoside-diphosphate reductase subunit M2 B

Compound/PW C040476 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine Monophosphate
stoichiometric coefficient: 1.0

Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Reference/UniProt P15531
type: UnificationXref
id: P15531
db: UniProt

Reference/HMDB HMDB0060639
type: UnificationXref
id: HMDB0060639
db: HMDB

Protein/2631 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ribonucleoside-diphosphate reductase subunit M2 B
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0060638
type: UnificationXref
id: HMDB0060638
db: HMDB

Reference/GENE ONTOLOGY GO 0005634
type: UnificationXref
id: GO:0005634
db: GENE ONTOLOGY

Deoxycytidylate deaminase
type: Protein
cellular location: Cytoplasm
entity reference: Deoxycytidylate deaminase

Reference/CAS 7664-41-7
type: UnificationXref
id: 7664-41-7
db: CAS

Reference/HMDB HMDB0061204
type: UnificationXref
id: HMDB0061204
db: HMDB

Equilibrative nucleoside transporter 1
type: Protein
entity reference: Equilibrative nucleoside transporter 1

Reference/ChemSpider 439295
type: UnificationXref
id: 439295
db: ChemSpider

Reference/HMDB HMDB0061262
type: UnificationXref
id: HMDB0061262
db: HMDB

Reference/PathWhiz PW000247
type: UnificationXref
id: PW000247
db: PathWhiz

Reference/UniProt P32321
type: UnificationXref
id: P32321
db: UniProt

Compound/PW C008951 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine
stoichiometric coefficient: 1.0

Adenosine triphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Adenosine triphosphate

Reference/UniProt Q8TCD5
type: UnificationXref
id: Q8TCD5
db: UniProt

Reference/PubChem-compound 222
type: UnificationXref
id: 222
db: PubChem-compound

Reference/KEGG Compound C00014
type: UnificationXref
id: C00014
db: KEGG Compound

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Compound/PW C040485 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Gemcitabine triphosphate
stoichiometric coefficient: 1.0

CTP synthase 1
type: ProteinReference
xref: Reference/UniProt P17812
organism: Homo sapiens

Ribonucleoside-diphosphate reductase 2
type: Complex
component stoichiometry: Protein/2631 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/104 Cytoplasm/Stoichiometry/1.0
cellular location: Cytoplasm
component: Ribonucleoside-diphosphate reductase large subunit
component: Ribonucleoside-diphosphate reductase subunit M2 B
name: SubPathwayInhibition

Reference/UniProt P04818
type: UnificationXref
id: P04818
db: UniProt

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

Gemcitabine
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Gemcitabine

Reference/UniProt P31350
type: UnificationXref
id: P31350
db: UniProt

Nucleus
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005634

3.1.3.-
type: BiochemicalReaction
participant stoichiometry: Compound/PW C040476 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C008951 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
right: Gemcitabine
right: Adenosine triphosphate
spontaneous: false
EC number: 3.1.3.-
left: Adenosine diphosphate
left: Gemcitabine Monophosphate
name: Adenosine diphosphate + Gemcitabine Monophosphate → Adenosine triphosphate + Gemcitabine
conversion direction: LEFT_TO_RIGHT

Sodium/nucleoside cotransporter 1
type: Protein
entity reference: Sodium/nucleoside cotransporter 1

Deoxycytidine kinase
type: Protein
cellular location: Nucleus
entity reference: Deoxycytidine kinase

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

Ribonucleoside-diphosphate reductase subunit M2 B
type: ProteinReference
xref: Reference/UniProt Q7LG56
organism: Homo sapiens

Nucleoside diphosphate kinase A
type: Complex
component stoichiometry: Protein/105 Cytoplasm/Stoichiometry/6.0
cellular location: Cytoplasm
component: Nucleoside diphosphate kinase A

Adenosine triphosphate
type: SmallMolecule
cellular location: Nucleus
entity reference: Adenosine triphosphate

Gemcitabine Monophosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Gemcitabine Monophosphate

Gemcitabine diphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Gemcitabine diphosphate
name: SubPathwayInhibitor

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

Adenosine diphosphate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00008
xref: Reference/PubChem-compound 6022
xref: Reference/ChemSpider 5800
xref: Reference/CAS 58-64-0
xref: Reference/BioCyc ADP
xref: Reference/ChEBI CHEBI 16761
xref: Reference/HMDB HMDB0001341
chemical formula: C10H15N5O10P2
molecular weight: 427.02942
structure: ChemicalStructure/PW C001034

ReactionCatalysis1955
type: Catalysis
controller: Deoxycytidylate deaminase
controlled: 3.5.4.12
control type: ACTIVATION

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

UMP-CMP kinase
type: Protein
cellular location: Nucleus
entity reference: UMP-CMP kinase

Nucleoside diphosphate kinase A
type: Protein
cellular location: Cytoplasm
entity reference: Nucleoside diphosphate kinase A

Difluorodeoxyuridine monophosphate inhibition of
type: Pathway
name: InhibitorySubPathway

Reference/UniProt Q99808
type: UnificationXref
id: Q99808
db: UniProt

Transport223
type: Transport
participant stoichiometry: Compound/PW C008951 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C008951 Extracellular Space/Stoichiometry/1.0
right: Gemcitabine
spontaneous: false
left: Gemcitabine
name: Gemcitabine (→) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Cell, Cytoplasm
conversion direction: LEFT_TO_RIGHT

Gemcitabine Monophosphate
type: SmallMolecule
cellular location: Nucleus
entity reference: Gemcitabine Monophosphate

Gemcitabine Di- and Triphosphate inhibit Ribonucleoside-diphosphate reductase
type: Pathway
name: InhibitorySubPathway

Gemcitabine Triphosphate inhibits CTP synthase 1
type: Pathway
name: InhibitorySubPathway

Incorporation into DNA and RNA
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway744
pathway component: SubPathwayInteraction744
name: SubPathway