Reference/ChEBI CHEBI 16015
type: UnificationXref
id: CHEBI:16015
db: ChEBI

Reference/PubChem-compound 6022
type: UnificationXref
id: 6022
db: PubChem-compound

Formimidoyltransferase-cyclodeaminase
type: Complex
component stoichiometry: Protein/7729 Cytoplasm/Stoichiometry/8.0
cellular location: Cytoplasm
component: Formimidoyltransferase-cyclodeaminase

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Unknown
type: Protein
cellular location: Cytoplasm
entity reference: Unknown

Methylenetetrahydrofolate reductase
type: Protein
cellular location: Cytoplasm
entity reference: Methylenetetrahydrofolate reductase

Reference/ChEBI CHEBI 16134
type: UnificationXref
id: CHEBI:16134
db: ChEBI

Compound/PW C000774 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: 10-Formyltetrahydrofolate
stoichiometric coefficient: 1.0

Compound/PW C000035 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ammonia
stoichiometric coefficient: 1.0

Reference/PubChem-compound 439153
type: UnificationXref
id: 439153
db: PubChem-compound

Compound/PW C000143 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADP
stoichiometric coefficient: 1.0

Tetrahydrofolic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Tetrahydrofolic acid
name: SubPathwayOutput
name: SubPathwayInput

Reference/CAS 58-05-9
type: UnificationXref
id: 58-05-9
db: CAS

Reference/BioCyc 10-FORMYL-THF
type: UnificationXref
id: 10-FORMYL-THF
db: BioCyc

10-Formyltetrahydrofolate
type: SmallMolecule
cellular location: Mitochondrion
entity reference: 10-Formyltetrahydrofolate

L-Glutamic acid
type: SmallMolecule
cellular location: Lysosome
entity reference: L-Glutamic acid

Reference/PathWhiz PW000056
type: UnificationXref
id: PW000056
db: PathWhiz

Reference/ChEBI CHEBI 20506
type: UnificationXref
id: CHEBI:20506
db: ChEBI

Reference/CAS 7444-29-3
type: UnificationXref
id: 7444-29-3
db: CAS

Dihydrofolic acid
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00415
xref: Reference/BioCyc DIHYDROFOLATE
xref: Reference/ChEBI CHEBI 15633
xref: Reference/ChemSpider 89228
xref: Reference/PubChem-compound 98792
xref: Reference/HMDB HMDB0001056
xref: Reference/CAS 4033-27-6
chemical formula: C19H21N7O6
molecular weight: 443.15533
structure: ChemicalStructure/PW C000831

Reference/ChEBI CHEBI 17119
type: UnificationXref
id: CHEBI:17119
db: ChEBI

Reference/KEGG Compound C00440
type: UnificationXref
id: C00440
db: KEGG Compound

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

Reference/KEGG Compound C00445
type: UnificationXref
id: C00445
db: KEGG Compound

Compound/PW C001178 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5,10-Methylene-THF
stoichiometric coefficient: 1.0

Reference/PathWhiz PW000052
type: UnificationXref
id: PW000052
db: PathWhiz

Reference/BioCyc 5-METHYL-THF
type: UnificationXref
id: 5-METHYL-THF
db: BioCyc

2.1.2.9
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000774 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: RNA/18 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: RNA/17 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Mitochondrion/Stoichiometry/1.0
right: N-formylmethionyl-tRNA(fMet)
right: Tetrahydrofolic acid
spontaneous: false
EC number: 2.1.2.9
left: L-Methionyl-tRNA(Met)
left: 10-Formyltetrahydrofolate
name: 10-Formyltetrahydrofolate + L-Methionyl-tRNA(Met) → N-formylmethionyl-tRNA(fMet) + Tetrahydrofolic acid
conversion direction: LEFT_TO_RIGHT

3.4.19.9
type: BiochemicalReaction
participant stoichiometry: Compound/PW C002955 Lysosome/Stoichiometry/1.0
participant stoichiometry: Compound/PW C002954 Lysosome/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000095 Lysosome/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Lysosome/Stoichiometry/1.0
right: L-Glutamic acid
right: Tetrahydrofolyl-[Glu](2)
spontaneous: false
EC number: 3.4.19.9
left: Water
left: Tetrahydrofolyl-[Glu](n)
name: Tetrahydrofolyl-[Glu](n) + Water → L-Glutamic acid + Tetrahydrofolyl-[Glu](2)
conversion direction: LEFT_TO_RIGHT

Reference/UniProt Q6PEM8
type: UnificationXref
id: Q6PEM8
db: UniProt

Reference/ChemSpider 18714427
type: UnificationXref
id: 18714427
db: ChemSpider

Reaction2572
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001144 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001045 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000721 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001178 Mitochondrion/Stoichiometry/1.0
right: NAD
right: 5,10-Methylene-THF
spontaneous: false
left: 5,10-Methenyltetrahydrofolic acid
left: NADH
name: 5,10-Methenyltetrahydrofolic acid + NADH ↔ 5,10-Methylene-THF + NAD
conversion direction: REVERSIBLE

6.3.2.17
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000095 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C002954 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C002955 Cytoplasm/Stoichiometry/1.0
right: Tetrahydrofolyl-[Glu](n)
right: Adenosine diphosphate
right: Phosphate
spontaneous: false
EC number: 6.3.2.17
left: Adenosine triphosphate
left: L-Glutamic acid
left: Tetrahydrofolyl-[Glu](2)
name: Adenosine triphosphate + L-Glutamic acid + Tetrahydrofolyl-[Glu](2) → Adenosine diphosphate + Phosphate + Tetrahydrofolyl-[Glu](n)
conversion direction: LEFT_TO_RIGHT

Protein/7726 Cytoplasm/Stoichiometry/2.0
type: Stoichiometry
physical entity: C-1-tetrahydrofolate synthase, cytoplasmic
stoichiometric coefficient: 2.0

Reference/ChemSpider 390680
type: UnificationXref
id: 390680
db: ChemSpider

Reference/PathWhiz PW000043
type: UnificationXref
id: PW000043
db: PathWhiz

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

Reference/ChEBI CHEBI 16474
type: UnificationXref
id: CHEBI:16474
db: ChEBI

Reference/PathWhiz PW000160
type: UnificationXref
id: PW000160
db: PathWhiz

Carbon dioxide
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Carbon dioxide

Reference/PubChem-compound 439175
type: UnificationXref
id: 439175
db: PubChem-compound

Compound/PW C000092 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Formic acid
stoichiometric coefficient: 1.0

Gamma-glutamyl hydrolase
type: Protein
cellular location: Lysosome
entity reference: Gamma-glutamyl hydrolase

Reference/PathWhiz PW000157
type: UnificationXref
id: PW000157
db: PathWhiz

Reference/BioCyc METHYLENE-THF
type: UnificationXref
id: METHYLENE-THF
db: BioCyc

Reference/ChemSpider 1010
type: UnificationXref
id: 1010
db: ChemSpider

Reference/KEGG Compound C00101
type: UnificationXref
id: C00101
db: KEGG Compound

Reference/PubChem-compound 6037
type: UnificationXref
id: 6037
db: PubChem-compound

N-Formyl-L-glutamic acid
type: SmallMoleculeReference
xref: Reference/BioCyc CPD-600
xref: Reference/PubChem-compound 439376
xref: Reference/CAS 1681-96-5
xref: Reference/ChEBI CHEBI 48309
xref: Reference/KEGG Compound C01045
xref: Reference/HMDB HMDB0003470
xref: Reference/ChemSpider 388496
chemical formula: C6H9NO5
molecular weight: 175.04807
structure: ChemicalStructure/PW C001903

Mus musculus
type: BioSource
xref: Reference/TAXONOMY 10090

Compound/PW C000414 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Reference/PubChem-compound 45479706
type: UnificationXref
id: 45479706
db: PubChem-compound

L-Glutamic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: L-Glutamic acid
name: SubPathwayOutput

Dihydrofolate reductase
type: Complex
component stoichiometry: Protein/7725 Cytoplasm/Stoichiometry/2.0
cellular location: Cytoplasm
component: Unknown

Tetrahydrofolyl-[Glu](n)
type: SmallMolecule
cellular location: Lysosome
entity reference: Tetrahydrofolyl-[Glu](n)

Reference/KEGG Compound C00234
type: UnificationXref
id: C00234
db: KEGG Compound

Reference/BioCyc 5-FORMYL-THF
type: UnificationXref
id: 5-FORMYL-THF
db: BioCyc

Compound/PW C001178 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5,10-Methylene-THF
stoichiometric coefficient: 1.0

Reference/PubChem-compound 122347
type: UnificationXref
id: 122347
db: PubChem-compound

Formimidoyltransferase-cyclodeaminase
type: Protein
cellular location: Cytoplasm
entity reference: Formimidoyltransferase-cyclodeaminase

Reference/KEGG Compound C00009
type: UnificationXref
id: C00009
db: KEGG Compound

Reference/KEGG Compound C00008
type: UnificationXref
id: C00008
db: KEGG Compound

Reference/KEGG Compound C00006
type: UnificationXref
id: C00006
db: KEGG Compound

5-Methyltetrahydrofolic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: 5-Methyltetrahydrofolic acid
name: SubPathwayOutput

N-Formyl-L-glutamic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: N-Formyl-L-glutamic acid

Reference/ChEBI CHEBI 18009
type: UnificationXref
id: CHEBI:18009
db: ChEBI

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Reference/KEGG Compound C00005
type: UnificationXref
id: C00005
db: KEGG Compound

Reference/ChEBI CHEBI 18367
type: UnificationXref
id: CHEBI:18367
db: ChEBI

ChemicalStructure/PW C001903
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)CC[C@H](NC=O)C(O)=O

Reference/KEGG Compound C00004
type: UnificationXref
id: C00004
db: KEGG Compound

Reference/CAS 135-16-0
type: UnificationXref
id: 135-16-0
db: CAS

Reference/ChemSpider 1032
type: UnificationXref
id: 1032
db: ChemSpider

Reference/KEGG Compound C00003
type: UnificationXref
id: C00003
db: KEGG Compound

NADP
type: SmallMolecule
cellular location: Cytoplasm
entity reference: NADP

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Reference/ChemSpider 388320
type: UnificationXref
id: 388320
db: ChemSpider

Compound/PW C002954 Lysosome/Stoichiometry/1.0
type: Stoichiometry
physical entity: Tetrahydrofolyl-[Glu](2)
stoichiometric coefficient: 1.0

Folate Metabolism
type: Pathway
pathway order: PathwayEnzymeReaction/2110980
pathway order: PathwayEnzymeReaction/2110982
pathway order: PathwayEnzymeReaction/2110981
pathway order: PathwayEnzymeReaction/2110984
pathway order: PathwayEnzymeReaction/2110983
pathway order: PathwayEnzymeReaction/2110986
pathway order: PathwaySubPathway/107922
pathway order: PathwayEnzymeReaction/2110985
pathway order: PathwaySubPathway/107923
pathway order: PathwayEnzymeReaction/2110988
pathway order: PathwayEnzymeReaction/2110987
pathway order: PathwayEnzymeReaction/2110989
pathway order: PathwayEnzymeReaction/2110991
pathway order: PathwayEnzymeReaction/2110990
pathway order: PathwayEnzymeReaction/2110993
pathway order: PathwayEnzymeReaction/2110992
pathway order: PathwayEnzymeReaction/2110995
pathway order: PathwaySubPathway/107931
pathway order: PathwayEnzymeReaction/2110994
pathway order: PathwaySubPathway/107932
pathway order: PathwaySubPathway/107933
pathway order: PathwayEnzymeReaction/2110996
pathway order: PathwayTransport/2110998
pathway order: PathwaySubPathway/107934
pathway order: PathwayTransport/2110997
pathway order: PathwaySubPathway/107930
pathway order: PathwaySubPathway/107928
pathway order: PathwaySubPathway/107929
pathway order: PathwaySubPathway/107924
pathway order: PathwaySubPathway/107925
pathway order: PathwaySubPathway/107926
pathway order: PathwaySubPathway/107927
pathway component: TransportCatalysis284
pathway component: 1.5.1.6
pathway component: ReactionCatalysis2534
pathway component: Reaction2563
pathway component: ReactionCatalysis2533
pathway component: Methionine Metabolism
pathway component: 1.5.1.3
pathway component: ReactionCatalysis2536
pathway component: Reaction2561
pathway component: ReactionCatalysis2535
pathway component: ReactionCatalysis2538
pathway component: ReactionCatalysis2537
pathway component: Purine Metabolism
pathway component: Glutamate Metabolism
pathway component: ReactionCatalysis2539
pathway component: Transport338
pathway component: Transport337
pathway component: Reaction2568
pathway component: 6.3.2.17
pathway component: ReactionCatalysis2530
pathway component: Reaction2566
pathway component: 1.5.1.20
pathway component: Reaction2564
pathway component: ReactionCatalysis2532
pathway component: ReactionCatalysis2531
pathway component: 6.3.3.2
pathway component: 2.1.2.9
pathway component: Histidine Metabolism
pathway component: ReactionCatalysis2525
pathway component: 3.4.19.9
pathway component: Reaction2572
pathway component: ReactionCatalysis2527
pathway component: ReactionCatalysis2526
pathway component: ReactionCatalysis2529
pathway component: ReactionCatalysis2528
pathway component: ReactionCatalysis2541
pathway component: ReactionCatalysis2540
pathway component: Glycine and Serine Metabolism
pathway component: Pyrimidine Metabolism
xref: Reference/SMPDB SMP0000801
xref: Reference/PathWhiz PW000778
organism: Mus musculus

Phosphate
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Phosphate

Compound/PW C001179 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5-Formiminotetrahydrofolic acid
stoichiometric coefficient: 1.0

Compound/PW C002955 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Tetrahydrofolyl-[Glu](n)
stoichiometric coefficient: 1.0

Tetrahydrofolyl-[Glu](n)
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Tetrahydrofolyl-[Glu](n)

NAD
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15846
xref: Reference/HMDB HMDB0000902
xref: Reference/CAS 53-84-9
xref: Reference/KEGG Compound C00003
xref: Reference/PubChem-compound 5893
xref: Reference/BioCyc NAD
xref: Reference/ChemSpider 5682
chemical formula: C21H27N7O14P2
molecular weight: 663.10913
structure: ChemicalStructure/PW C000721

Adenosine triphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Adenosine triphosphate

Reference/KEGG Compound C00011
type: UnificationXref
id: C00011
db: KEGG Compound

Reference/HMDB HMDB0000902
type: UnificationXref
id: HMDB0000902
db: HMDB

Reference/KEGG Compound C00014
type: UnificationXref
id: C00014
db: KEGG Compound

Compound/PW C002954 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Tetrahydrofolyl-[Glu](2)
stoichiometric coefficient: 1.0

Protein/7729 Cytoplasm/Stoichiometry/8.0
type: Stoichiometry
physical entity: Formimidoyltransferase-cyclodeaminase
stoichiometric coefficient: 8.0

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Reference/BioCyc NAD(P)
type: UnificationXref
id: NAD(P)
db: BioCyc

Reference/ChemSpider 11267158
type: UnificationXref
id: 11267158
db: ChemSpider

Hydrogen Ion
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00080
xref: Reference/ChEBI CHEBI 15378
xref: Reference/HMDB HMDB0059597
xref: Reference/PubChem-compound 1038
xref: Reference/ChemSpider 1010
chemical formula: H
molecular weight: 1.007825
structure: ChemicalStructure/PW C040034

Proton-coupled folate transporter
type: Protein
entity reference: Proton-coupled folate transporter

Reference/KEGG Compound C00143
type: UnificationXref
id: C00143
db: KEGG Compound

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

Reference/CAS 53-84-9
type: UnificationXref
id: 53-84-9
db: CAS

Reference/KEGG Compound C00025
type: UnificationXref
id: C00025
db: KEGG Compound

Reference/CAS 3432-99-3
type: UnificationXref
id: 3432-99-3
db: CAS

Monofunctional C1-tetrahydrofolate synthase, mitochondrial
type: Complex
component stoichiometry: Protein/7727 Mitochondrion/Stoichiometry/2.0
cellular location: Mitochondrion
component: Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Reference/SMPDB SMP0000801
type: UnificationXref
id: SMP0000801
db: SMPDB

Reference/KEGG Compound C03541
type: UnificationXref
id: C03541
db: KEGG Compound

ChemicalStructure/PW C001144
type: ChemicalStructure
structure format: SMILES

Compound/PW C001316 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Carbon dioxide
stoichiometric coefficient: 1.0

Reference/UniProt Q9Z0L8
type: UnificationXref
id: Q9Z0L8
db: UniProt

Adenosine diphosphate
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Adenosine diphosphate

Reference/PubChem-compound 98792
type: UnificationXref
id: 98792
db: PubChem-compound

L-Methionyl-tRNA(Met)
type: RnaReference
xref: Reference/ChEBI CHEBI 16635
organism: Mus musculus

Reference/HMDB HMDB0001341
type: UnificationXref
id: HMDB0001341
db: HMDB

N-formylmethionyl-tRNA(fMet)
type: RnaReference
xref: Reference/ChEBI CHEBI 17119
organism: Mus musculus

Reference/PubChem-compound 644350
type: UnificationXref
id: 644350
db: PubChem-compound

Compound/PW C000076 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Folic acid
stoichiometric coefficient: 1.0

ChemicalStructure/PW C001034
type: ChemicalStructure
structure format: SMILES

Reference/BioCyc DIHYDROFOLATE
type: UnificationXref
id: DIHYDROFOLATE
db: BioCyc

Reference/HMDB HMDB0001354
type: UnificationXref
id: HMDB0001354
db: HMDB

L-Methionyl-tRNA(Met)
type: Rna
cellular location: Mitochondrion
entity reference: L-Methionyl-tRNA(Met)

Reference/HMDB HMDB0000148
type: UnificationXref
id: HMDB0000148
db: HMDB

Reference/UniProt Q922D8
type: UnificationXref
id: Q922D8
db: UniProt

Compound/PW C001144 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADH
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0000142
type: UnificationXref
id: HMDB0000142
db: HMDB

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Reference/KEGG Compound C00058
type: UnificationXref
id: C00058
db: KEGG Compound

Reference/HMDB HMDB0001487
type: UnificationXref
id: HMDB0001487
db: HMDB

NAD
type: SmallMolecule
cellular location: Mitochondrion
entity reference: NAD

Reference/BioCyc AMMONIA
type: UnificationXref
id: AMMONIA
db: BioCyc

ChemicalStructure/PW C000035
type: ChemicalStructure
structure format: SMILES
structure data: N

Reference/CAS 56-86-0
type: UnificationXref
id: 56-86-0
db: CAS

Protein/7734 Lysosome/Stoichiometry/2.0
type: Stoichiometry
physical entity: Gamma-glutamyl hydrolase
stoichiometric coefficient: 2.0

Reference/BioCyc FORMATE
type: UnificationXref
id: FORMATE
db: BioCyc

Adenosine diphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Adenosine diphosphate

Methylenetetrahydrofolate reductase
type: Complex
component stoichiometry: Protein/7732 Cytoplasm/Stoichiometry/2.0
cellular location: Cytoplasm
component: Methylenetetrahydrofolate reductase

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

10-Formyltetrahydrofolate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000972
xref: Reference/PubChem-compound 122347
xref: Reference/ChemSpider 109092
xref: Reference/ChEBI CHEBI 15637
xref: Reference/CAS 2800-34-2
xref: Reference/BioCyc 10-FORMYL-THF
xref: Reference/KEGG Compound C00234
chemical formula: C20H23N7O7
molecular weight: 473.1659
structure: ChemicalStructure/PW C000774

Reference/PubChem-compound 442163
type: UnificationXref
id: 442163
db: PubChem-compound

Tetrahydrofolyl-[Glu](2)
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0006825
xref: Reference/ChEBI CHEBI 28624
xref: Reference/PubChem-compound 442163
xref: Reference/KEGG Compound C03541
xref: Reference/ChemSpider 390680
chemical formula: C24H30N8O9
molecular weight: 574.21356
structure: ChemicalStructure/PW C002954

Tetrahydrofolyl-[Glu](n)
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0006826
xref: Reference/PubChem-compound 45479706
xref: Reference/ChemSpider 21231977
xref: Reference/KEGG Compound C03541
xref: Reference/ChEBI CHEBI 27650
chemical formula: C29H37N9O12
molecular weight: 703.25616
structure: ChemicalStructure/PW C002955

Reference/CAS 2800-34-2
type: UnificationXref
id: 2800-34-2
db: CAS

Reference/UniProt Q9D799
type: UnificationXref
id: Q9D799
db: UniProt

Reference/PubChem-compound 530
type: UnificationXref
id: 530
db: PubChem-compound

Reference/CAS 4033-27-6
type: UnificationXref
id: 4033-27-6
db: CAS

Reference/HMDB HMDB0006825
type: UnificationXref
id: HMDB0006825
db: HMDB

Reference/HMDB HMDB0006826
type: UnificationXref
id: HMDB0006826
db: HMDB

Ammonia
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Ammonia

RNA/17 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Methionyl-tRNA(Met)
stoichiometric coefficient: 1.0

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

5,10-Methylene-THF
type: SmallMolecule
cellular location: Cytoplasm
entity reference: 5,10-Methylene-THF
name: SubPathwayInput

Reference/ChemSpider 5800
type: UnificationXref
id: 5800
db: ChemSpider

Reference/KEGG Compound C01045
type: UnificationXref
id: C01045
db: KEGG Compound

Reference/UniProt Q3TYX5
type: UnificationXref
id: Q3TYX5
db: UniProt

Carbon dioxide
type: SmallMoleculeReference
xref: Reference/PubChem-compound 280
xref: Reference/ChEBI CHEBI 16526
xref: Reference/ChemSpider 274
xref: Reference/HMDB HMDB0001967
xref: Reference/CAS 124-38-9
xref: Reference/KEGG Compound C00011
chemical formula: CO2
molecular weight: 43.98983
structure: ChemicalStructure/PW C001316

Reference/KEGG Compound C00080
type: UnificationXref
id: C00080
db: KEGG Compound

Glutamate Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway107927
pathway component: SubPathwayInteraction107927
xref: Reference/SMPDB SMP0000072
xref: Reference/PathWhiz PW000003
name: SubPathway

Reference/HMDB HMDB0000051
type: UnificationXref
id: HMDB0000051
db: HMDB

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

ChemicalStructure/PW C000095
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CCC(O)=O)C(O)=O

ChemicalStructure/PW C000092
type: ChemicalStructure
structure format: SMILES
structure data: OC=O

Protein/7732 Cytoplasm/Stoichiometry/2.0
type: Stoichiometry
physical entity: Methylenetetrahydrofolate reductase
stoichiometric coefficient: 2.0

Reference/CAS 14265-44-2
type: UnificationXref
id: 14265-44-2
db: CAS

Reference/BioCyc 5,10-methenyl-thf
type: UnificationXref
id: 5,10-methenyl-thf
db: BioCyc

Reference/CAS 58-64-0
type: UnificationXref
id: 58-64-0
db: CAS

Reference/KEGG Compound C03479
type: UnificationXref
id: C03479
db: KEGG Compound

Methionyl-tRNA formyltransferase, mitochondrial
type: Protein
cellular location: Mitochondrion
entity reference: Methionyl-tRNA formyltransferase, mitochondrial

Reference/ChEBI CHEBI 209153
type: UnificationXref
id: CHEBI:209153
db: ChEBI

ChemicalStructure/PW C001199
type: ChemicalStructure
structure format: SMILES

Reference/BioCyc FOLATE
type: UnificationXref
id: FOLATE
db: BioCyc

ChemicalStructure/PW C001079
type: ChemicalStructure
structure format: SMILES

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
type: Protein
cellular location: Mitochondrion
entity reference: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

10-Formyltetrahydrofolate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: 10-Formyltetrahydrofolate
name: SubPathwayInput

Reference/HMDB HMDB0001396
type: UnificationXref
id: HMDB0001396
db: HMDB

Compound/PW C000721 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: NAD
stoichiometric coefficient: 1.0

Reference/CAS 64-18-6
type: UnificationXref
id: 64-18-6
db: CAS

Compound/PW C001079 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5-Methyltetrahydrofolic acid
stoichiometric coefficient: 1.0

ChemicalStructure/PW C001045
type: ChemicalStructure
structure format: SMILES

Compound/PW C001045 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5,10-Methenyltetrahydrofolic acid
stoichiometric coefficient: 1.0

Reference/PubChem-compound 22833512
type: UnificationXref
id: 22833512
db: PubChem-compound

ChemicalStructure/PW C000076
type: ChemicalStructure
structure format: SMILES

N-formylmethionyl-tRNA(fMet)
type: Rna
cellular location: Mitochondrion
entity reference: N-formylmethionyl-tRNA(fMet)

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Gamma-glutamyl hydrolase
type: Complex
component stoichiometry: Protein/7734 Lysosome/Stoichiometry/2.0
cellular location: Lysosome
component: Gamma-glutamyl hydrolase

Transport338
type: Transport
participant stoichiometry: Compound/PW C000076 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000076 Cytoplasm/Stoichiometry/1.0
right: Folic acid
spontaneous: false
left: Folic acid
name: Folic acid (↔) Transport: Mus musculus, Extracellular Space to Mus musculus, Cell, Cytoplasm
conversion direction: LEFT_TO_RIGHT

Compound/PW C000092 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Formic acid
stoichiometric coefficient: 1.0

Transport337
type: Transport
participant stoichiometry: Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Mitochondrion/Stoichiometry/1.0
right: Tetrahydrofolic acid
spontaneous: false
left: Tetrahydrofolic acid
name: Tetrahydrofolic acid (→) Transport: Mus musculus, Cell, Cytoplasm to Mus musculus, Cell, Mitochondrion
conversion direction: LEFT_TO_RIGHT

Reference/BioCyc NADH
type: UnificationXref
id: NADH
db: BioCyc

5-Formiminotetrahydrofolic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: 5-Formiminotetrahydrofolic acid
name: SubPathwayOutput

Reference/BioCyc NAD
type: UnificationXref
id: NAD
db: BioCyc

Reference/ChemSpider 5815
type: UnificationXref
id: 5815
db: ChemSpider

Reference/HMDB HMDB0059597
type: UnificationXref
id: HMDB0059597
db: HMDB

Reference/ChemSpider 559356
type: UnificationXref
id: 559356
db: ChemSpider

Folylpolyglutamate synthase, mitochondrial
type: Protein
cellular location: Cytoplasm
entity reference: Folylpolyglutamate synthase, mitochondrial

5,10-Methenyltetrahydrofolic acid
type: SmallMolecule
cellular location: Mitochondrion
entity reference: 5,10-Methenyltetrahydrofolic acid

Compound/PW C001420 Lysosome/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Formic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Formic acid

ChemicalStructure/PW C001179
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C001178
type: ChemicalStructure
structure format: SMILES

Reference/CAS 134-35-0
type: UnificationXref
id: 134-35-0
db: CAS

NAD
type: SmallMolecule
cellular location: Cytoplasm
entity reference: NAD

Reference/HMDB HMDB0003470
type: UnificationXref
id: HMDB0003470
db: HMDB

Reference/HMDB HMDB0001056
type: UnificationXref
id: HMDB0001056
db: HMDB

Compound/PW C002955 Lysosome/Stoichiometry/1.0
type: Stoichiometry
physical entity: Tetrahydrofolyl-[Glu](n)
stoichiometric coefficient: 1.0

Reference/ChemSpider 30572
type: UnificationXref
id: 30572
db: ChemSpider

Reference/ChemSpider 17215925
type: UnificationXref
id: 17215925
db: ChemSpider

Folic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Folic acid

Dihydrofolic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Dihydrofolic acid
name: SubPathwayInput

ReactionCatalysis2534
type: Catalysis
controller: C-1-tetrahydrofolate synthase, cytoplasmic
controlled: Reaction2566
control type: ACTIVATION

ReactionCatalysis2533
type: Catalysis
controller: 5-formyltetrahydrofolate cyclo-ligase
controlled: 6.3.3.2
control type: ACTIVATION

Methionine Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway107929
pathway order: SubPathwayInteractionSteps/SubPathway107924
pathway component: SubPathwayInteraction107924
pathway component: SubPathwayInteraction107929
xref: Reference/PathWhiz PW000056
xref: Reference/SMPDB SMP0000033
name: SubPathway

ReactionCatalysis2536
type: Catalysis
controller: Formimidoyltransferase-cyclodeaminase
controlled: Reaction2568
control type: ACTIVATION

ReactionCatalysis2535
type: Catalysis
controller: Methylenetetrahydrofolate reductase
controlled: 1.5.1.20
control type: ACTIVATION

ReactionCatalysis2538
type: Catalysis
controller: Folylpolyglutamate synthase, mitochondrial
controlled: 6.3.2.17
control type: ACTIVATION

ReactionCatalysis2537
type: Catalysis
controller: Folylpolyglutamate synthase, mitochondrial
controlled: 6.3.2.17
control type: ACTIVATION

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

ReactionCatalysis2539
type: Catalysis
controller: Gamma-glutamyl hydrolase
controlled: 3.4.19.9
control type: ACTIVATION

Ammonia
type: SmallMoleculeReference
xref: Reference/BioCyc AMMONIA
xref: Reference/CAS 7664-41-7
xref: Reference/HMDB HMDB0000051
xref: Reference/PubChem-compound 222
xref: Reference/KEGG Compound C00014
xref: Reference/ChEBI CHEBI 16134
xref: Reference/ChemSpider 217
chemical formula: H3N
molecular weight: 17.026548
structure: ChemicalStructure/PW C000035

Compound/PW C000831 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Dihydrofolic acid
stoichiometric coefficient: 1.0

ReactionCatalysis2530
type: Catalysis
controller: Formimidoyltransferase-cyclodeaminase
controlled: Reaction2563
control type: ACTIVATION

ReactionCatalysis2532
type: Catalysis
controller: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
controlled: Reaction2564
control type: ACTIVATION

ReactionCatalysis2531
type: Catalysis
controller: C-1-tetrahydrofolate synthase, cytoplasmic
controlled: Reaction2564
control type: ACTIVATION

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

5-formyltetrahydrofolate cyclo-ligase
type: Protein
cellular location: Cytoplasm
entity reference: 5-formyltetrahydrofolate cyclo-ligase

Histidine Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway107922
pathway order: SubPathwayInteractionSteps/SubPathway107928
pathway component: SubPathwayInteraction107922
pathway component: SubPathwayInteraction107928
xref: Reference/PathWhiz PW000043
xref: Reference/SMPDB SMP0000044
name: SubPathway

Water
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Water

Reference/ChEBI CHEBI 15846
type: UnificationXref
id: CHEBI:15846
db: ChEBI

Folic acid
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Folic acid

Reference/BioCyc ADP
type: UnificationXref
id: ADP
db: BioCyc

ReactionCatalysis2541
type: Catalysis
controller: Methionyl-tRNA formyltransferase, mitochondrial
controlled: 2.1.2.9
control type: ACTIVATION

ReactionCatalysis2540
type: Catalysis
controller: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
controlled: Reaction2572
control type: ACTIVATION

Pyrimidine Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway107931
pathway order: SubPathwayInteractionSteps/SubPathway107926
pathway component: SubPathwayInteraction107926
pathway component: SubPathwayInteraction107931
xref: Reference/SMPDB SMP0000046
xref: Reference/PathWhiz PW000160
name: SubPathway

Reference/TAXONOMY 10090
type: UnificationXref
id: 10090
db: TAXONOMY

Reference/CAS 53-59-8
type: UnificationXref
id: 53-59-8
db: CAS

Lysosome
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005764

Compound/PW C000095 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic acid
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 48309
type: UnificationXref
id: CHEBI:48309
db: ChEBI

Reference/PubChem-compound 1038
type: UnificationXref
id: 1038
db: PubChem-compound

Reference/UniProt Q8R0Y6
type: UnificationXref
id: Q8R0Y6
db: UniProt

Reference/PubChem-compound 143
type: UnificationXref
id: 143
db: PubChem-compound

Reference/ChemSpider 278
type: UnificationXref
id: 278
db: ChemSpider

Tetrahydrofolic acid
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 20506
xref: Reference/ChemSpider 18714427
xref: Reference/HMDB HMDB0001846
xref: Reference/PubChem-compound 13781857
xref: Reference/BioCyc THF
xref: Reference/CAS 135-16-0
xref: Reference/KEGG Compound C00101
chemical formula: C19H23N7O6
molecular weight: 445.171
structure: ChemicalStructure/PW C001221

Purine Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway107923
pathway order: SubPathwayInteractionSteps/SubPathway107932
pathway component: SubPathwayInteraction107923
pathway component: SubPathwayInteraction107932
xref: Reference/SMPDB SMP0000050
xref: Reference/PathWhiz PW000052
name: SubPathway

Reference/ChemSpider 274
type: UnificationXref
id: 274
db: ChemSpider

Hydrogen Ion
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Hydrogen Ion

Reference/ChEBI CHEBI 16908
type: UnificationXref
id: CHEBI:16908
db: ChEBI

Phosphate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00009
xref: Reference/PubChem-compound 1061
xref: Reference/CAS 14265-44-2
xref: Reference/HMDB HMDB0001429
xref: Reference/BioCyc CPD-8587
xref: Reference/ChEBI CHEBI 18367
xref: Reference/ChemSpider 1032
chemical formula: H3O4P
molecular weight: 97.9769
structure: ChemicalStructure/PW C001104

Reference/UniProt P48760
type: UnificationXref
id: P48760
db: UniProt

Reference/HMDB HMDB0001533
type: UnificationXref
id: HMDB0001533
db: HMDB

Adenosine triphosphate
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Adenosine triphosphate

Reference/HMDB HMDB0001534
type: UnificationXref
id: HMDB0001534
db: HMDB

NADPH
type: SmallMoleculeReference
xref: Reference/CAS 53-57-6
xref: Reference/HMDB HMDB0000221
xref: Reference/PubChem-compound 22833512
xref: Reference/BioCyc NADPH
xref: Reference/ChemSpider 17215925
xref: Reference/KEGG Compound C00005
xref: Reference/ChEBI CHEBI 16474
chemical formula: C21H30N7O17P3
molecular weight: 745.0911
structure: ChemicalStructure/PW C000146

Reference/ChemSpider 140
type: UnificationXref
id: 140
db: ChemSpider

NADP
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00006
xref: Reference/CAS 53-59-8
xref: Reference/BioCyc NAD(P)
xref: Reference/HMDB HMDB0000217
xref: Reference/ChEBI CHEBI 18009
xref: Reference/PubChem-compound 5886
xref: Reference/ChemSpider 5675
chemical formula: C21H28N7O17P3
molecular weight: 743.07544
structure: ChemicalStructure/PW C000143

Mitochondrion
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005739

Reference/BioCyc THF
type: UnificationXref
id: THF
db: BioCyc

Reference/ChEBI CHEBI 15641
type: UnificationXref
id: CHEBI:15641
db: ChEBI

Reference/BioCyc NADPH
type: UnificationXref
id: NADPH
db: BioCyc

TransportCatalysis284
type: Catalysis
controller: Proton-coupled folate transporter
controlled: Transport338
control type: ACTIVATION

Compound/PW C040034 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/ChemSpider 217
type: UnificationXref
id: 217
db: ChemSpider

ChemicalStructure/PW C001221
type: ChemicalStructure
structure format: SMILES

Reference/CAS 58-68-4
type: UnificationXref
id: 58-68-4
db: CAS

Water
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Water

ChemicalStructure/PW C001104
type: ChemicalStructure
structure format: SMILES
structure data: OP(O)(O)=O

5,10-Methenyltetrahydrofolic acid
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001354
xref: Reference/CAS 7444-29-3
xref: Reference/BioCyc 5,10-methenyl-thf
xref: Reference/ChEBI CHEBI 15636
xref: Reference/PubChem-compound 644350
xref: Reference/ChemSpider 559356
xref: Reference/KEGG Compound C00445
chemical formula: C20H21N7O6
molecular weight: 455.15533
structure: ChemicalStructure/PW C001045

Reference/HMDB HMDB0000217
type: UnificationXref
id: HMDB0000217
db: HMDB

Compound/PW C000146 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADPH
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 15637
type: UnificationXref
id: CHEBI:15637
db: ChEBI

Reference/CAS 124-38-9
type: UnificationXref
id: 124-38-9
db: CAS

Reference/PathWhiz PW000778
type: UnificationXref
id: PW000778
db: PathWhiz

Reference/ChEBI CHEBI 15639
type: UnificationXref
id: CHEBI:15639
db: ChEBI

Reference/GENE ONTOLOGY GO 0005764
type: UnificationXref
id: GO:0005764
db: GENE ONTOLOGY

Reference/SMPDB SMP0000046
type: UnificationXref
id: SMP0000046
db: SMPDB

Folic acid
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00504
xref: Reference/BioCyc FOLATE
xref: Reference/ChemSpider 5815
xref: Reference/CAS 59-30-3
xref: Reference/ChEBI CHEBI 27470
xref: Reference/HMDB HMDB0000121
xref: Reference/PubChem-compound 6037
chemical formula: C19H19N7O6
molecular weight: 441.13968
structure: ChemicalStructure/PW C000076

Reference/SMPDB SMP0000044
type: UnificationXref
id: SMP0000044
db: SMPDB

Tetrahydrofolyl-[Glu](2)
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Tetrahydrofolyl-[Glu](2)

Reference/HMDB HMDB0001429
type: UnificationXref
id: HMDB0001429
db: HMDB

ChemicalStructure/PW C000143
type: ChemicalStructure
structure format: SMILES

Reference/SMPDB SMP0000050
type: UnificationXref
id: SMP0000050
db: SMPDB

Reference/HMDB HMDB0000221
type: UnificationXref
id: HMDB0000221
db: HMDB

Compound/PW C040034 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Compound/PW C000095 Lysosome/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic acid
stoichiometric coefficient: 1.0

ChemicalStructure/PW C000146
type: ChemicalStructure
structure format: SMILES

5,10-Methylene-THF
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001533
xref: Reference/CAS 3432-99-3
xref: Reference/PubChem-compound 439175
xref: Reference/ChEBI CHEBI 15636
xref: Reference/KEGG Compound C00143
xref: Reference/BioCyc METHYLENE-THF
xref: Reference/ChemSpider 388320
chemical formula: C20H23N7O6
molecular weight: 457.171
structure: ChemicalStructure/PW C001178

5-Formiminotetrahydrofolic acid
type: SmallMoleculeReference
xref: Reference/CAS 2311-81-1
xref: Reference/HMDB HMDB0001534
xref: Reference/BioCyc 5-FORMIMINO-THF
xref: Reference/ChemSpider 11267158
xref: Reference/ChEBI CHEBI 15639
xref: Reference/KEGG Compound C00664
xref: Reference/PubChem-compound 530
chemical formula: C20H24N8O6
molecular weight: 472.1819
structure: ChemicalStructure/PW C001179

Cytosolic 10-formyltetrahydrofolate dehydrogenase
type: Protein
cellular location: Cytoplasm
entity reference: Cytosolic 10-formyltetrahydrofolate dehydrogenase

Reference/BioCyc GLT
type: UnificationXref
id: GLT
db: BioCyc

Reference/CAS 7664-41-7
type: UnificationXref
id: 7664-41-7
db: CAS

Glycine and Serine Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway107930
pathway order: SubPathwayInteractionSteps/SubPathway107934
pathway order: SubPathwayInteractionSteps/SubPathway107933
pathway order: SubPathwayInteractionSteps/SubPathway107925
pathway component: SubPathwayInteraction107934
pathway component: SubPathwayInteraction107925
pathway component: SubPathwayInteraction107930
pathway component: SubPathwayInteraction107933
xref: Reference/PathWhiz PW000157
xref: Reference/SMPDB SMP0000004
name: SubPathway

Reference/CAS 53-57-6
type: UnificationXref
id: 53-57-6
db: CAS

ChemicalStructure/PW C001316
type: ChemicalStructure
structure format: SMILES
structure data: O=C=O

Compound/PW C001034 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine diphosphate
stoichiometric coefficient: 1.0

Reference/PubChem-compound 222
type: UnificationXref
id: 222
db: PubChem-compound

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
type: Complex
component stoichiometry: Protein/7730 Mitochondrion/Stoichiometry/2.0
cellular location: Mitochondrion
component: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

NADH
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001487
xref: Reference/CAS 58-68-4
xref: Reference/PubChem-compound 439153
xref: Reference/BioCyc NADH
xref: Reference/ChemSpider 388299
xref: Reference/KEGG Compound C00004
xref: Reference/ChEBI CHEBI 16908
chemical formula: C21H29N7O14P2
molecular weight: 665.12476
structure: ChemicalStructure/PW C001144

Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Reference/BioCyc 5-FORMIMINO-THF
type: UnificationXref
id: 5-FORMIMINO-THF
db: BioCyc

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

Reference/CAS 1681-96-5
type: UnificationXref
id: 1681-96-5
db: CAS

Reference/HMDB HMDB0001562
type: UnificationXref
id: HMDB0001562
db: HMDB

Reference/ChEBI CHEBI 15633
type: UnificationXref
id: CHEBI:15633
db: ChEBI

C-1-tetrahydrofolate synthase, cytoplasmic
type: Complex
component stoichiometry: Protein/7726 Cytoplasm/Stoichiometry/2.0
cellular location: Cytoplasm
component: C-1-tetrahydrofolate synthase, cytoplasmic

Reference/ChEBI CHEBI 15636
type: UnificationXref
id: CHEBI:15636
db: ChEBI

5,10-Methenyltetrahydrofolic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: 5,10-Methenyltetrahydrofolic acid

Monofunctional C1-tetrahydrofolate synthase, mitochondrial
type: Protein
cellular location: Mitochondrion
entity reference: Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Reference/SMPDB SMP0000072
type: UnificationXref
id: SMP0000072
db: SMPDB

Compound/PW C001144 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADH
stoichiometric coefficient: 1.0

ReactionCatalysis2525
type: Catalysis
controller: Dihydrofolate reductase
controlled: 1.5.1.3
control type: ACTIVATION

Reference/GENE ONTOLOGY GO 0005739
type: UnificationXref
id: GO:0005739
db: GENE ONTOLOGY

ReactionCatalysis2527
type: Catalysis
controller: C-1-tetrahydrofolate synthase, cytoplasmic
controlled: Reaction2561
control type: ACTIVATION

Adenosine diphosphate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00008
xref: Reference/PubChem-compound 6022
xref: Reference/ChemSpider 5800
xref: Reference/CAS 58-64-0
xref: Reference/BioCyc ADP
xref: Reference/ChEBI CHEBI 16761
xref: Reference/HMDB HMDB0001341
chemical formula: C10H15N5O10P2
molecular weight: 427.02942
structure: ChemicalStructure/PW C001034

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

ReactionCatalysis2526
type: Catalysis
controller: Dihydrofolate reductase
controlled: 1.5.1.3
control type: ACTIVATION

ReactionCatalysis2529
type: Catalysis
controller: Cytosolic 10-formyltetrahydrofolate dehydrogenase
controlled: 1.5.1.6
control type: ACTIVATION

ReactionCatalysis2528
type: Catalysis
controller: Monofunctional C1-tetrahydrofolate synthase, mitochondrial
controlled: Reaction2561
control type: ACTIVATION

Compound/PW C001420 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0000121
type: UnificationXref
id: HMDB0000121
db: HMDB

Protein/7725 Cytoplasm/Stoichiometry/2.0
type: Stoichiometry
physical entity: Unknown
stoichiometric coefficient: 2.0

Compound/PW C000076 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Folic acid
stoichiometric coefficient: 1.0

NADH
type: SmallMolecule
cellular location: Cytoplasm
entity reference: NADH

Reference/BioCyc CPD-8587
type: UnificationXref
id: CPD-8587
db: BioCyc

ChemicalStructure/PW C000721
type: ChemicalStructure
structure format: SMILES

Reference/ChemSpider 5682
type: UnificationXref
id: 5682
db: ChemSpider

Reference/UniProt P18155
type: UnificationXref
id: P18155
db: UniProt

RNA/18 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: N-formylmethionyl-tRNA(fMet)
stoichiometric coefficient: 1.0

Compound/PW C001104 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphate
stoichiometric coefficient: 1.0

Reference/SMPDB SMP0000004
type: UnificationXref
id: SMP0000004
db: SMPDB

SubPathwayInteraction107934
type: Interaction
name: SubPathway107934Reaction
name: SubPathwayReaction
participant: 5,10-Methylene-THF

SubPathwayInteraction107930
type: Interaction
name: SubPathwayReaction
name: SubPathway107930Reaction
participant: 5,10-Methylene-THF

SubPathwayInteraction107931
type: Interaction
name: SubPathway107931Reaction
name: SubPathwayReaction
participant: 5,10-Methylene-THF

SubPathwayInteraction107932
type: Interaction
name: SubPathway107932Reaction
name: SubPathwayReaction
participant: 10-Formyltetrahydrofolate

SubPathwayInteraction107933
type: Interaction
name: SubPathwayReaction
name: SubPathway107933Reaction
participant: Tetrahydrofolic acid

Reference/ChemSpider 21231977
type: UnificationXref
id: 21231977
db: ChemSpider

Reference/UniProt F6Z4F8
type: UnificationXref
id: F6Z4F8
db: UniProt

Reference/ChemSpider 5675
type: UnificationXref
id: 5675
db: ChemSpider

Reference/UniProt Q3V3R1
type: UnificationXref
id: Q3V3R1
db: UniProt

Reference/PubChem-compound 18971002
type: UnificationXref
id: 18971002
db: PubChem-compound

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

5,10-Methylene-THF
type: SmallMolecule
cellular location: Mitochondrion
entity reference: 5,10-Methylene-THF
name: SubPathwayInput

NADPH
type: SmallMolecule
cellular location: Cytoplasm
entity reference: NADPH

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

Reference/ChEBI CHEBI 16635
type: UnificationXref
id: CHEBI:16635
db: ChEBI

Reference/ChemSpider 388299
type: UnificationXref
id: 388299
db: ChemSpider

Hydrogen Ion
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Hydrogen Ion

Formic acid
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 30751
xref: Reference/ChemSpider 278
xref: Reference/PubChem-compound 18971002
xref: Reference/BioCyc FORMATE
xref: Reference/CAS 64-18-6
xref: Reference/KEGG Compound C00058
xref: Reference/HMDB HMDB0000142
chemical formula: CH2O2
molecular weight: 46.005478
structure: ChemicalStructure/PW C000092

Tetrahydrofolyl-[Glu](2)
type: SmallMolecule
cellular location: Lysosome
entity reference: Tetrahydrofolyl-[Glu](2)

Reference/ChEBI CHEBI 28624
type: UnificationXref
id: CHEBI:28624
db: ChEBI

Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Tetrahydrofolic acid
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 27650
type: UnificationXref
id: CHEBI:27650
db: ChEBI

L-Glutamic acid
type: SmallMoleculeReference
xref: Reference/PubChem-compound 33032
xref: Reference/ChEBI CHEBI 16015
xref: Reference/ChemSpider 30572
xref: Reference/HMDB HMDB0000148
xref: Reference/CAS 56-86-0
xref: Reference/BioCyc GLT
xref: Reference/KEGG Compound C00025
chemical formula: C5H9NO4
molecular weight: 147.05316
structure: ChemicalStructure/PW C000095

Reference/KEGG Compound C00504
type: UnificationXref
id: C00504
db: KEGG Compound

Reference/PubChem-compound 33032
type: UnificationXref
id: 33032
db: PubChem-compound

Compound/PW C001903 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: N-Formyl-L-glutamic acid
stoichiometric coefficient: 1.0

Water
type: SmallMolecule
cellular location: Lysosome
entity reference: Water

Formimidoyltransferase-cyclodeaminase
type: ProteinReference
xref: Reference/UniProt Q91XD4
organism: Mus musculus

Cytosolic 10-formyltetrahydrofolate dehydrogenase
type: ProteinReference
xref: Reference/UniProt Q8R0Y6
organism: Mus musculus

Monofunctional C1-tetrahydrofolate synthase, mitochondrial
type: ProteinReference
xref: Reference/UniProt Q3V3R1
organism: Mus musculus

C-1-tetrahydrofolate synthase, cytoplasmic
type: ProteinReference
xref: Reference/UniProt Q922D8
organism: Mus musculus

Unknown
type: ProteinReference
organism: Mus musculus

Reference/ChEBI CHEBI 16526
type: UnificationXref
id: CHEBI:16526
db: ChEBI

ChemicalStructure/PW C000831
type: ChemicalStructure
structure format: SMILES

Reference/ChEBI CHEBI 16761
type: UnificationXref
id: CHEBI:16761
db: ChEBI

Reference/UniProt Q91XD4
type: UnificationXref
id: Q91XD4
db: UniProt

N5-Formyl-THF
type: SmallMoleculeReference
xref: Reference/KEGG Compound C03479
xref: Reference/ChemSpider 140
xref: Reference/CAS 58-05-9
xref: Reference/HMDB HMDB0001562
xref: Reference/BioCyc 5-FORMYL-THF
xref: Reference/PubChem-compound 143
xref: Reference/ChEBI CHEBI 209153
chemical formula: C20H23N7O7
molecular weight: 473.1659
structure: ChemicalStructure/PW C001199

5-Methyltetrahydrofolic acid
type: SmallMoleculeReference
xref: Reference/BioCyc 5-METHYL-THF
xref: Reference/ChEBI CHEBI 15641
xref: Reference/PubChem-compound 439234
xref: Reference/CAS 134-35-0
xref: Reference/KEGG Compound C00440
xref: Reference/ChemSpider 388371
xref: Reference/HMDB HMDB0001396
chemical formula: C20H25N7O6
molecular weight: 459.18665
structure: ChemicalStructure/PW C001079

NADH
type: SmallMolecule
cellular location: Mitochondrion
entity reference: NADH

Reference/SMPDB SMP0000033
type: UnificationXref
id: SMP0000033
db: SMPDB

Compound/PW C000774 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: 10-Formyltetrahydrofolate
stoichiometric coefficient: 1.0

Proton-coupled folate transporter
type: ProteinReference
xref: Reference/UniProt Q6PEM8

Compound/PW C001199 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: N5-Formyl-THF
stoichiometric coefficient: 1.0

Methionyl-tRNA formyltransferase, mitochondrial
type: ProteinReference
xref: Reference/UniProt Q9D799
organism: Mus musculus

Gamma-glutamyl hydrolase
type: ProteinReference
xref: Reference/UniProt Q9Z0L8
organism: Mus musculus

Folylpolyglutamate synthase, mitochondrial
type: ProteinReference
xref: Reference/UniProt P48760
organism: Mus musculus

Methylenetetrahydrofolate reductase
type: ProteinReference
xref: Reference/UniProt F6Z4F8
organism: Mus musculus

Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine diphosphate
stoichiometric coefficient: 1.0

5-formyltetrahydrofolate cyclo-ligase
type: ProteinReference
xref: Reference/UniProt Q3TYX5
organism: Mus musculus

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
type: ProteinReference
xref: Reference/UniProt P18155
organism: Mus musculus

Reference/PubChem-compound 5893
type: UnificationXref
id: 5893
db: PubChem-compound

Formic acid
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Formic acid

Reference/PubChem-compound 439234
type: UnificationXref
id: 439234
db: PubChem-compound

Reference/HMDB HMDB0000972
type: UnificationXref
id: HMDB0000972
db: HMDB

Reference/ChEBI CHEBI 30751
type: UnificationXref
id: CHEBI:30751
db: ChEBI

ChemicalStructure/PW C000774
type: ChemicalStructure
structure format: SMILES

Reference/ChEBI CHEBI 27470
type: UnificationXref
id: CHEBI:27470
db: ChEBI

Protein/7730 Mitochondrion/Stoichiometry/2.0
type: Stoichiometry
physical entity: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
stoichiometric coefficient: 2.0

Compound/PW C001221 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Tetrahydrofolic acid
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Reference/ChEBI CHEBI 15378
type: UnificationXref
id: CHEBI:15378
db: ChEBI

ChemicalStructure/PW C040034
type: ChemicalStructure
structure format: SMILES
structure data: [H+]

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Reference/PubChem-compound 5886
type: UnificationXref
id: 5886
db: PubChem-compound

ChemicalStructure/PW C002954
type: ChemicalStructure
structure format: SMILES

Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Phosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Phosphate

ChemicalStructure/PW C002955
type: ChemicalStructure
structure format: SMILES

Compound/PW C001045 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5,10-Methenyltetrahydrofolic acid
stoichiometric coefficient: 1.0

Reference/PubChem-compound 280
type: UnificationXref
id: 280
db: PubChem-compound

SubPathwayInteraction107923
type: Interaction
name: SubPathwayReaction
name: SubPathway107923Reaction
participant: Tetrahydrofolic acid

Reference/KEGG Compound C00415
type: UnificationXref
id: C00415
db: KEGG Compound

SubPathwayInteraction107924
type: Interaction
name: SubPathway107924Reaction
name: SubPathwayReaction
participant: Tetrahydrofolic acid

SubPathwayInteraction107925
type: Interaction
name: SubPathwayReaction
name: SubPathway107925Reaction
participant: Tetrahydrofolic acid

SubPathwayInteraction107926
type: Interaction
name: SubPathway107926Reaction
name: SubPathwayReaction
participant: Dihydrofolic acid

SubPathwayInteraction107922
type: Interaction
name: SubPathwayReaction
name: SubPathway107922Reaction
participant: Tetrahydrofolic acid

Reference/PathWhiz PW000003
type: UnificationXref
id: PW000003
db: PathWhiz

Reference/ChemSpider 89228
type: UnificationXref
id: 89228
db: ChemSpider

Reference/ChemSpider 388371
type: UnificationXref
id: 388371
db: ChemSpider

Compound/PW C001104 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphate
stoichiometric coefficient: 1.0

SubPathwayInteraction107927
type: Interaction
name: SubPathway107927Reaction
name: SubPathwayReaction
participant: L-Glutamic acid

SubPathwayInteraction107928
type: Interaction
name: SubPathwayReaction
name: SubPathway107928Reaction
participant: 5-Formiminotetrahydrofolic acid

SubPathwayInteraction107929
type: Interaction
name: SubPathway107929Reaction
name: SubPathwayReaction
participant: 5-Methyltetrahydrofolic acid

Reference/ChemSpider 388496
type: UnificationXref
id: 388496
db: ChemSpider

Protein/7727 Mitochondrion/Stoichiometry/2.0
type: Stoichiometry
physical entity: Monofunctional C1-tetrahydrofolate synthase, mitochondrial
stoichiometric coefficient: 2.0

1.5.1.6
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000774 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001316 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000146 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
right: Carbon dioxide
right: Tetrahydrofolic acid
right: NADPH
spontaneous: false
EC number: 1.5.1.6
left: Water
left: 10-Formyltetrahydrofolate
left: NADP
name: 10-Formyltetrahydrofolate + NADP + Water → Carbon dioxide + NADPH + Tetrahydrofolic acid
conversion direction: LEFT_TO_RIGHT

Reaction2563
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001903 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000095 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001199 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
right: N-Formyl-L-glutamic acid
right: Tetrahydrofolic acid
spontaneous: false
left: N5-Formyl-THF
left: L-Glutamic acid
name: L-Glutamic acid + N5-Formyl-THF → N-Formyl-L-glutamic acid + Tetrahydrofolic acid
conversion direction: LEFT_TO_RIGHT

Reference/CAS 2311-81-1
type: UnificationXref
id: 2311-81-1
db: CAS

1.5.1.3
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000146 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000076 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000831 Cytoplasm/Stoichiometry/1.0
right: Folic acid
right: NADPH
spontaneous: false
EC number: 1.5.1.3
left: Dihydrofolic acid
left: NADP
name: Dihydrofolic acid + NADP ↔ Folic acid + NADPH
conversion direction: REVERSIBLE

Reaction2561
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000774 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000774 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000092 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000092 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Mitochondrion/Stoichiometry/1.0
right: Adenosine diphosphate
right: Phosphate
right: 10-Formyltetrahydrofolate
spontaneous: false
left: Formic acid
left: Adenosine triphosphate
left: Tetrahydrofolic acid
name: Adenosine triphosphate + Formic acid + Tetrahydrofolic acid → 10-Formyltetrahydrofolate + Adenosine diphosphate + Phosphate
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 439376
type: UnificationXref
id: 439376
db: PubChem-compound

Reaction2568
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001045 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000035 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001179 Cytoplasm/Stoichiometry/1.0
right: Ammonia
right: 5,10-Methenyltetrahydrofolic acid
spontaneous: false
left: 5-Formiminotetrahydrofolic acid
name: 5-Formiminotetrahydrofolic acid ↔ 5,10-Methenyltetrahydrofolic acid + Ammonia
conversion direction: REVERSIBLE

6.3.2.17
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000095 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C002954 Cytoplasm/Stoichiometry/1.0
right: Adenosine diphosphate
right: Phosphate
right: Tetrahydrofolyl-[Glu](2)
spontaneous: false
EC number: 6.3.2.17
left: Adenosine triphosphate
left: L-Glutamic acid
left: Tetrahydrofolic acid
name: Adenosine triphosphate + L-Glutamic acid + Tetrahydrofolic acid → Adenosine diphosphate + Phosphate + Tetrahydrofolyl-[Glu](2)
conversion direction: LEFT_TO_RIGHT

Reaction2566
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001045 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001178 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000146 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143 Cytoplasm/Stoichiometry/1.0
right: 5,10-Methylene-THF
right: NADP
spontaneous: false
left: 5,10-Methenyltetrahydrofolic acid
left: NADPH
name: 5,10-Methenyltetrahydrofolic acid + NADPH ↔ 5,10-Methylene-THF + NADP
conversion direction: REVERSIBLE

1.5.1.20
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001178 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000721 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001144 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001079 Cytoplasm/Stoichiometry/1.0
right: 5-Methyltetrahydrofolic acid
right: NAD
spontaneous: false
EC number: 1.5.1.20
left: 5,10-Methylene-THF
left: NADH
name: 5,10-Methylene-THF + NADH → 5-Methyltetrahydrofolic acid + NAD
conversion direction: LEFT_TO_RIGHT

Reaction2564
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000774 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001045 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000774 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001045 Mitochondrion/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Mitochondrion/Stoichiometry/1.0
right: Water
right: 5,10-Methenyltetrahydrofolic acid
spontaneous: false
left: Hydrogen Ion
left: 10-Formyltetrahydrofolate
name: 10-Formyltetrahydrofolate + Hydrogen Ion ↔ 5,10-Methenyltetrahydrofolic acid + Water
conversion direction: REVERSIBLE

6.3.3.2
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001045 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001199 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
right: 5,10-Methenyltetrahydrofolic acid
right: Adenosine diphosphate
right: Phosphate
spontaneous: false
EC number: 6.3.3.2
left: Adenosine triphosphate
left: N5-Formyl-THF
name: Adenosine triphosphate + N5-Formyl-THF → 5,10-Methenyltetrahydrofolic acid + Adenosine diphosphate + Phosphate
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 1061
type: UnificationXref
id: 1061
db: PubChem-compound

Reference/BioCyc CPD-600
type: UnificationXref
id: CPD-600
db: BioCyc

Reference/ChemSpider 109092
type: UnificationXref
id: 109092
db: ChemSpider

Reference/HMDB HMDB0001846
type: UnificationXref
id: HMDB0001846
db: HMDB

Reference/HMDB HMDB0001967
type: UnificationXref
id: HMDB0001967
db: HMDB

Reference/KEGG Compound C00664
type: UnificationXref
id: C00664
db: KEGG Compound

Tetrahydrofolic acid
type: SmallMolecule
cellular location: Mitochondrion
entity reference: Tetrahydrofolic acid
name: SubPathwayInput

C-1-tetrahydrofolate synthase, cytoplasmic
type: Protein
cellular location: Cytoplasm
entity reference: C-1-tetrahydrofolate synthase, cytoplasmic

1.5.1.3
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000146 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001221 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000831 Cytoplasm/Stoichiometry/1.0
right: NADPH
right: Dihydrofolic acid
spontaneous: false
EC number: 1.5.1.3
left: Tetrahydrofolic acid
left: NADP
name: NADP + Tetrahydrofolic acid ↔ Dihydrofolic acid + NADPH
conversion direction: REVERSIBLE

Reference/PubChem-compound 13781857
type: UnificationXref
id: 13781857
db: PubChem-compound

N5-Formyl-THF
type: SmallMolecule
cellular location: Cytoplasm
entity reference: N5-Formyl-THF

Compound/PW C000721 Mitochondrion/Stoichiometry/1.0
type: Stoichiometry
physical entity: NAD
stoichiometric coefficient: 1.0

Reference/CAS 59-30-3
type: UnificationXref
id: 59-30-3
db: CAS