Reference/ChEBI CHEBI 16015
type: UnificationXref
id: CHEBI:16015
db: ChEBI

Glutamate/aspartate transport system permease protein gltK
type: Protein
cellular location: Inner Membrane
entity reference: Glutamate/aspartate transport system permease protein gltK

Reference/ChemSpider 5605
type: UnificationXref
id: 5605
db: ChemSpider

Protein/6127 Cytosol/Stoichiometry/10.0
type: Stoichiometry
physical entity: Pyrroline-5-carboxylate reductase
stoichiometric coefficient: 10.0

Reference/PubChem-compound 6022
type: UnificationXref
id: 6022
db: PubChem-compound

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Reference/PubChem-compound 439153
type: UnificationXref
id: 439153
db: PubChem-compound

Reference/CAS 13254-53-0
type: UnificationXref
id: 13254-53-0
db: CAS

Reference/ChEBI CHEBI 27391
type: UnificationXref
id: CHEBI:27391
db: ChEBI

4-Hydroxyproline
type: SmallMolecule
cellular location: Cytosol
entity reference: 4-Hydroxyproline

Reference/ChemSpider 50
type: UnificationXref
id: 50
db: ChemSpider

Compound/PW C040034/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 16389
type: UnificationXref
id: CHEBI:16389
db: ChEBI

L-Glutamic acid 5-phosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 17798
xref: Reference/ChemSpider 167893
xref: Reference/BioCyc L-GLUTAMATE-5-P
xref: Reference/HMDB HMDB0001228
xref: Reference/CAS 13254-53-0
xref: Reference/PubChem-compound 193475
xref: Reference/KEGG Compound C03287
chemical formula: C5H10NO7P
molecular weight: 227.01949
structure: ChemicalStructure/PW C000951

NAD
type: SmallMolecule
entity reference: NAD

Protein/6047/Stoichiometry/4.0
type: Stoichiometry
physical entity: Glutamate 5-kinase
stoichiometric coefficient: 4.0

Glutamate/aspartate periplasmic-binding protein
type: Protein
cellular location: Inner Membrane
entity reference: Glutamate/aspartate periplasmic-binding protein

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

L-erythro-4-Hydroxyglutamate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C05947
xref: Reference/PubChem-compound 8231
xref: Reference/ChEBI CHEBI 197035
chemical formula: C5H9NO5
molecular weight: 163.04807
structure: ChemicalStructure/PW C282129

Reference/ChEBI CHEBI 17232
type: UnificationXref
id: CHEBI:17232
db: ChEBI

Compound/PW C000095/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic acid
stoichiometric coefficient: 1.0

Bifunctional protein putA
type: Protein
entity reference: Bifunctional protein putA

Glyoxylic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Glyoxylic acid

Unknown
type: Protein
cellular location: Cytosol
entity reference: Unknown

Compound/PW C000075 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Glyoxylic acid
stoichiometric coefficient: 1.0

Reference/BioCyc PRO
type: UnificationXref
id: PRO
db: BioCyc

(4R)-4-Hydroxy-2-oxoglutarate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C05946
xref: Reference/PubChem-compound 8230
xref: Reference/ChEBI CHEBI 4083
chemical formula: C5H6O6
molecular weight: 162.01643
structure: ChemicalStructure/PW C282130

Protein/5973/Stoichiometry/2.0
type: Stoichiometry
physical entity: Bifunctional protein putA
stoichiometric coefficient: 2.0

Reference/ChEBI CHEBI 17203
type: UnificationXref
id: CHEBI:17203
db: ChEBI

Compound/PW C001034/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine diphosphate
stoichiometric coefficient: 1.0

Glycolate and Glyoxylate Degradation
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway741727
pathway component: SubPathwayInteraction741727
xref: Reference/SMPDB SMP0000847
xref: Reference/PathWhiz PW000827
name: SubPathway

L-Glutamic acid 5-phosphate
type: SmallMolecule
entity reference: L-Glutamic acid 5-phosphate

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

NADH
type: SmallMolecule
cellular location: Cytosol
entity reference: NADH

Glutamate/aspartate transport system permease protein gltJ
type: Protein
cellular location: Inner Membrane
entity reference: Glutamate/aspartate transport system permease protein gltJ

3.4.11.23
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000106 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C006813 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000078 Cytoplasm/Stoichiometry/1.0
right: Glycine
right: L-Proline
spontaneous: false
EC number: 3.4.11.23
left: Water
left: Prolylglycine
name: Prolylglycine + Water → Glycine + L-Proline
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 16474
type: UnificationXref
id: CHEBI:16474
db: ChEBI

Reference/ChemSpider 952
type: UnificationXref
id: 952
db: ChemSpider

Reference/UniProt P37095
type: UnificationXref
id: P37095
db: UniProt

Bifunctional protein PutA
type: Protein
cellular location: Cytosol
entity reference: Bifunctional protein PutA

Reference/ChemSpider 1010
type: UnificationXref
id: 1010
db: ChemSpider

Glutamate/aspartate transport ATP-binding protein gltL
type: Protein
cellular location: Inner Membrane
entity reference: Glutamate/aspartate transport ATP-binding protein gltL

SubPathwayInteraction741729
type: Interaction
name: SubPathwayReaction
name: SubPathway741729Reaction
participant: Pyruvic acid

SubPathwayInteraction741727
type: Interaction
name: SubPathway741727Reaction
name: SubPathwayReaction
participant: Glyoxylic acid

Reference/SMPDB SMP0000847
type: UnificationXref
id: SMP0000847
db: SMPDB

Compound/PW C000146/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADPH
stoichiometric coefficient: 1.0

Reference/BioCyc L-GLUTAMATE-5-P
type: UnificationXref
id: L-GLUTAMATE-5-P
db: BioCyc

ReactionCoupledTransportCatalysis136
type: Catalysis
controller: glutamate / aspartate ABC transporter
controlled: ReactionCoupledTransport102
control type: ACTIVATION

Hydrogen Ion
type: SmallMolecule
entity reference: Hydrogen Ion

Pyrroline-5-carboxylate reductase
type: ProteinReference
xref: Reference/UniProt P0A9L8

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

Compound/PW C001144 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADH
stoichiometric coefficient: 1.0

Adenosine triphosphate
type: SmallMolecule
entity reference: Adenosine triphosphate

Reference/BioCyc SUC
type: UnificationXref
id: SUC
db: BioCyc

Reference/PubChem-compound 193305
type: UnificationXref
id: 193305
db: PubChem-compound

Pyruvate Decarboxylation to Acetyl-CoA
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway741729
pathway component: SubPathwayInteraction741729
xref: Reference/SMPDB SMP0002095
xref: Reference/PathWhiz PW002083
name: SubPathway

Reference/KEGG Compound C00009
type: UnificationXref
id: C00009
db: KEGG Compound

Reference/ChemSpider 1159
type: UnificationXref
id: 1159
db: ChemSpider

Reference/ChemSpider 4307
type: UnificationXref
id: 4307
db: ChemSpider

Reference/KEGG Compound C00008
type: UnificationXref
id: C00008
db: KEGG Compound

Reference/KEGG Compound C00007
type: UnificationXref
id: C00007
db: KEGG Compound

Reference/KEGG Compound C00006
type: UnificationXref
id: C00006
db: KEGG Compound

Reference/ChEBI CHEBI 18009
type: UnificationXref
id: CHEBI:18009
db: ChEBI

Hydrogen Ion
type: SmallMolecule
cellular location: Cytosol
entity reference: Hydrogen Ion

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Reference/ChemSpider 1030
type: UnificationXref
id: 1030
db: ChemSpider

BiologicalState235
type: BioSource
xref: Reference/TAXONOMY 83333
name: Escherichia coli (strain K12)

Reference/KEGG Compound C00005
type: UnificationXref
id: C00005
db: KEGG Compound

Glutamate/aspartate transport ATP-binding protein gltL
type: ProteinReference
xref: Reference/UniProt P0AAG3
organism: Escherichia coli

Reference/ChEBI CHEBI 18367
type: UnificationXref
id: CHEBI:18367
db: ChEBI

Reference/KEGG Compound C00004
type: UnificationXref
id: C00004
db: KEGG Compound

Reference/ChemSpider 1032
type: UnificationXref
id: 1032
db: ChemSpider

Reference/KEGG Compound C00003
type: UnificationXref
id: C00003
db: KEGG Compound

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Reference/ChemSpider 1031
type: UnificationXref
id: 1031
db: ChemSpider

Reference/CAS 496-92-4
type: UnificationXref
id: 496-92-4
db: CAS

ReactionCoupledTransport102
type: TransportWithBiochemicalReaction
participant stoichiometry: Compound/PW C040034 Inner Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Inner Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000095 Inner Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Inner Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034 Inner Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104 Inner Membrane/Stoichiometry/1.0
right: Phosphate
right: L-Glutamic acid
right: Adenosine diphosphate
right: Hydrogen Ion
spontaneous: false
left: Water
left: Adenosine triphosphate
left: L-Glutamic acid
name: Adenosine triphosphate + L-Glutamic acid + Water → Adenosine diphosphate + Hydrogen Ion + L-Glutamic acid + Phosphate
conversion direction: LEFT_TO_RIGHT

Compound/PW C000106/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Proline
stoichiometric coefficient: 1.0

Reference/KEGG Compound C05947
type: UnificationXref
id: C05947
db: KEGG Compound

NAD
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15846
xref: Reference/HMDB HMDB0000902
xref: Reference/CAS 53-84-9
xref: Reference/KEGG Compound C00003
xref: Reference/PubChem-compound 5893
xref: Reference/BioCyc NAD
xref: Reference/ChemSpider 5682
chemical formula: C21H27N7O14P2
molecular weight: 663.10913
structure: ChemicalStructure/PW C000721

Reference/KEGG Compound C05946
type: UnificationXref
id: C05946
db: KEGG Compound

Reference/PubChem-compound 447920
type: UnificationXref
id: 447920
db: PubChem-compound

Reference/KEGG Compound C00011
type: UnificationXref
id: C00011
db: KEGG Compound

Reference/HMDB HMDB0000902
type: UnificationXref
id: HMDB0000902
db: HMDB

L-Proline
type: SmallMolecule
cellular location: Cytosol
entity reference: L-Proline

Reference/BioCyc NAD(P)
type: UnificationXref
id: NAD(P)
db: BioCyc

Reaction179292
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000095/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001034/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000951/Stoichiometry/1.0
right: Adenosine diphosphate
right: L-Glutamic acid 5-phosphate
spontaneous: false
left: L-Glutamic acid
left: Adenosine triphosphate
name: Adenosine triphosphate + L-Glutamic acid → Adenosine diphosphate + L-Glutamic acid 5-phosphate
conversion direction: LEFT_TO_RIGHT

Reference/TAXONOMY 562
type: UnificationXref
id: 562
db: TAXONOMY

Pyruvic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Pyruvic acid

Hydrogen Ion
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00080
xref: Reference/ChEBI CHEBI 15378
xref: Reference/HMDB HMDB0059597
xref: Reference/PubChem-compound 1038
xref: Reference/ChemSpider 1010
chemical formula: H
molecular weight: 1.007825
structure: ChemicalStructure/PW C040034

ChemicalStructure/PW C282129
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](C[C@@H](O)C(O)=O)C(O)=O

Compound/PW C006813 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Prolylglycine
stoichiometric coefficient: 1.0

Proline Metabolism
type: Pathway
pathway order: PathwaySubPathway/741729
pathway order: PathwayEnzymeReaction/8922030
pathway order: PathwaySubPathway/741727
pathway order: PathwayEnzymeReaction/7733
pathway order: PathwayEnzymeReaction/4902
pathway order: PathwayEnzymeReaction/4903
pathway order: PathwayEnzymeReaction/8921904
pathway order: PathwayEnzymeReaction/4898
pathway order: PathwayEnzymeReaction/2122397
pathway order: PathwayEnzymeReaction/8921875
pathway order: PathwayEnzymeReaction/9073925
pathway order: PathwayEnzymeReaction/4900
pathway order: PathwayEnzymeReaction/8921954
pathway order: PathwayEnzymeReaction/4899
pathway order: PathwayEnzymeReaction/8417
pathway order: PathwayEnzymeReaction/8921980
pathway component: ReactionCoupledTransportCatalysis136
pathway component: Glycolate and Glyoxylate Degradation
pathway component: ReactionCatalysis2707
pathway component: ReactionCatalysis2709
pathway component: ReactionCatalysis2708
pathway component: 3.4.11.23
pathway component: ReactionCatalysis2030481
pathway component: Reaction179292
pathway component: Reaction1960070
pathway component: Pyruvate Decarboxylation to Acetyl-CoA
pathway component: ReactionCatalysis1991874
pathway component: Reaction2722
pathway component: Reaction1960072
pathway component: 1.5.5.2
pathway component: Reaction2721
pathway component: 1.5.1.2
pathway component: Reaction2717
pathway component: ReactionCatalysis1991870
pathway component: ReactionCatalysis1991873
pathway component: 1.2.1.41
pathway component: ReactionCatalysis1991872
pathway component: ReactionCatalysis169197
pathway component: ReactionCatalysis2705
pathway component: ReactionCoupledTransport102
pathway component: Reaction1960065
pathway component: ReactionCatalysis1991869
pathway component: Reaction1960066
xref: Reference/PathWhiz PW000794
xref: Reference/SMPDB SMP0000815
organism: Escherichia coli

ReactionCatalysis1991870
type: Catalysis
controller: pyrroline-5-carboxylate reductase
controlled: Reaction1960066
control type: ACTIVATION

ReactionCatalysis1991873
type: Catalysis
controller: aspartate transaminase
controlled: Reaction1960070
control type: ACTIVATION

ReactionCatalysis1991872
type: Catalysis
controller: Bifunctional protein PutA
controlled: 1.5.5.2
control type: ACTIVATION

L-erythro-4-Hydroxyglutamate
type: SmallMolecule
cellular location: Cytosol
entity reference: L-erythro-4-Hydroxyglutamate

Reference/KEGG Compound C00022
type: UnificationXref
id: C00022
db: KEGG Compound

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

Reference/CAS 53-84-9
type: UnificationXref
id: 53-84-9
db: CAS

Reference/KEGG Compound C00148
type: UnificationXref
id: C00148
db: KEGG Compound

Glutamate/aspartate periplasmic-binding protein
type: ProteinReference
xref: Reference/UniProt P37902
organism: Escherichia coli

L-Glutamic acid
type: SmallMolecule
cellular location: Cytosol
entity reference: L-Glutamic acid

Reference/KEGG Compound C00026
type: UnificationXref
id: C00026
db: KEGG Compound

Reference/KEGG Compound C00025
type: UnificationXref
id: C00025
db: KEGG Compound

Reference/CAS 298-12-4
type: UnificationXref
id: 298-12-4
db: CAS

Protein/5916/Stoichiometry/4.0
type: Stoichiometry
physical entity: Gamma-glutamyl phosphate reductase
stoichiometric coefficient: 4.0

Compound/PW C000143/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADP
stoichiometric coefficient: 1.0

ChemicalStructure/PW C282130
type: ChemicalStructure
structure format: SMILES
structure data: [H][C@@](O)(CC(=O)C(O)=O)C(O)=O

Compound/PW C042948/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ubiquinol-1
stoichiometric coefficient: 1.0

ReactionCatalysis1991869
type: Catalysis
controller: Unknown
controlled: Reaction1960065
control type: ACTIVATION

Reference/UniProt P0AAG3
type: UnificationXref
id: P0AAG3
db: UniProt

Reference/PubChem-compound 760
type: UnificationXref
id: 760
db: PubChem-compound

Glutamate 5-kinase
type: ProteinReference
xref: Reference/UniProt P0A7B5

Compound/PW C000164 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Pyruvic acid
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00037
type: UnificationXref
id: C00037
db: KEGG Compound

Reference/KEGG Compound C00399
type: UnificationXref
id: C00399
db: KEGG Compound

Reference/PathWhiz PW002083
type: UnificationXref
id: PW002083
db: PathWhiz

ChemicalStructure/PW C001144
type: ChemicalStructure
structure format: SMILES

Reference/CAS 328-50-7
type: UnificationXref
id: 328-50-7
db: CAS

Reference/HMDB HMDB0000254
type: UnificationXref
id: HMDB0000254
db: HMDB

Water
type: SmallMolecule
entity reference: Water

ChemicalStructure/PW C000174
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)CCC(O)=O

Reference/HMDB HMDB0001228
type: UnificationXref
id: HMDB0001228
db: HMDB

ReactionCatalysis1991874
type: Catalysis
controller: Unknown
controlled: Reaction1960072
control type: ACTIVATION

Reference/HMDB HMDB0001341
type: UnificationXref
id: HMDB0001341
db: HMDB

Reference/SMPDB SMP0000815
type: UnificationXref
id: SMP0000815
db: SMPDB

Prolylglycine
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Prolylglycine

L-Glutamic γ-semialdehyde
type: SmallMolecule
entity reference: L-Glutamic γ-semialdehyde

Reference/ChemSpider 128566
type: UnificationXref
id: 128566
db: ChemSpider

Reference/KEGG Compound C00042
type: UnificationXref
id: C00042
db: KEGG Compound

Reference/KEGG Compound C00048
type: UnificationXref
id: C00048
db: KEGG Compound

Reference/ChEBI CHEBI 4083
type: UnificationXref
id: CHEBI:4083
db: ChEBI

ChemicalStructure/PW C001034
type: ChemicalStructure
structure format: SMILES

Reference/HMDB HMDB0000148
type: UnificationXref
id: HMDB0000148
db: HMDB

Compound/PW C000134 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Oxoglutaric acid
stoichiometric coefficient: 1.0

ReactionCatalysis2705
type: Catalysis
controller: glutamate-5-semialdehyde dehydrogenase
controlled: 1.2.1.41
control type: ACTIVATION

Protein/5824 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Aspartate aminotransferase
stoichiometric coefficient: 2.0

ReactionCatalysis2707
type: Catalysis
controller: pyrroline-5-carboxylate reductase
controlled: 1.5.1.2
control type: ACTIVATION

ReactionCatalysis2709
type: Catalysis
controller: proline dehydrogenase
controlled: Reaction2722
control type: ACTIVATION

ReactionCatalysis2708
type: Catalysis
controller: proline dehydrogenase
controlled: 1.5.5.2
control type: ACTIVATION

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

ChemicalStructure/PW C042948
type: ChemicalStructure
structure format: SMILES
structure data: COC1=C(O)C(C)=C(CC=C(C)C)C(O)=C1OC

Reference/HMDB HMDB0001487
type: UnificationXref
id: HMDB0001487
db: HMDB

Reference/BioCyc GLYOX
type: UnificationXref
id: GLYOX
db: BioCyc

glutamate-5-semialdehyde dehydrogenase
type: Complex
component stoichiometry: Protein/5916/Stoichiometry/4.0
component: Gamma-glutamyl phosphate reductase

ChemicalStructure/PW C006813
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)CNC(=O)[C@@H]1CCCN1

γ-glutamyl kinase
type: Complex
component stoichiometry: Protein/6047/Stoichiometry/4.0
component: Glutamate 5-kinase

Aspartate aminotransferase
type: Protein
cellular location: Cytosol
entity reference: Aspartate aminotransferase

Reference/HMDB HMDB0001369
type: UnificationXref
id: HMDB0001369
db: HMDB

ChemicalStructure/PW C001003
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)C1CCC=N1

Reference/CAS 56-86-0
type: UnificationXref
id: 56-86-0
db: CAS

proline dehydrogenase
type: Complex
component stoichiometry: Protein/5973/Stoichiometry/2.0
component: Bifunctional protein putA

pyrroline-5-carboxylate reductase
type: Complex
component stoichiometry: Protein/6127/Stoichiometry/10.0
component: Pyrroline-5-carboxylate reductase

NAD
type: SmallMolecule
cellular location: Cytosol
entity reference: NAD

Reference/UniProt P0AER3
type: UnificationXref
id: P0AER3
db: UniProt

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

Reference/UniProt P0AER5
type: UnificationXref
id: P0AER5
db: UniProt

Reference/CAS 56-40-6
type: UnificationXref
id: 56-40-6
db: CAS

Glutamate/aspartate transport system permease protein gltK
type: ProteinReference
xref: Reference/UniProt P0AER5
organism: Escherichia coli

Reference/HMDB HMDB0011178
type: UnificationXref
id: HMDB0011178
db: HMDB

Glutamate/aspartate transport system permease protein gltJ
type: ProteinReference
xref: Reference/UniProt P0AER3
organism: Escherichia coli

Reference/KEGG Compound C01157
type: UnificationXref
id: C01157
db: KEGG Compound

Reference/HMDB HMDB0002104
type: UnificationXref
id: HMDB0002104
db: HMDB

Reference/HMDB HMDB0001377
type: UnificationXref
id: HMDB0001377
db: HMDB

ChemicalStructure/PW C000164
type: ChemicalStructure
structure format: SMILES
structure data: CC(=O)C(O)=O

Peptidase B
type: Complex
component stoichiometry: Protein/113962 Cytoplasm/Stoichiometry/6.0
cellular location: Cytoplasm
component: Peptidase B

Reference/HMDB HMDB0000162
type: UnificationXref
id: HMDB0000162
db: HMDB

Bifunctional protein PutA
type: Complex
component stoichiometry: Protein/107695 Cytosol/Stoichiometry/2.0
cellular location: Cytosol
component: Bifunctional protein PutA

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Compound/PW C001347/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ubiquinone-1
stoichiometric coefficient: 1.0

L-Proline
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 17203
xref: Reference/ChemSpider 128566
xref: Reference/BioCyc PRO
xref: Reference/CAS 147-85-3
xref: Reference/KEGG Compound C00148
xref: Reference/HMDB HMDB0000162
xref: Reference/PubChem-compound 145742
chemical formula: C5H9NO2
molecular weight: 115.06333
structure: ChemicalStructure/PW C000106

Reference/ChemSpider 5800
type: UnificationXref
id: 5800
db: ChemSpider

Compound/PW C001003/Stoichiometry/1.0
type: Stoichiometry
physical entity: 1-Pyrroline-5-carboxylic acid
stoichiometric coefficient: 1.0

Reference/KEGG Compound C01165
type: UnificationXref
id: C01165
db: KEGG Compound

Carbon dioxide
type: SmallMoleculeReference
xref: Reference/PubChem-compound 280
xref: Reference/ChEBI CHEBI 16526
xref: Reference/ChemSpider 274
xref: Reference/HMDB HMDB0001967
xref: Reference/CAS 124-38-9
xref: Reference/KEGG Compound C00011
chemical formula: CO2
molecular weight: 43.98983
structure: ChemicalStructure/PW C001316

Phosphate
type: SmallMolecule
entity reference: Phosphate

Reference/PubChem-compound 145742
type: UnificationXref
id: 145742
db: PubChem-compound

Ubiquinone-1
type: SmallMolecule
entity reference: Ubiquinone-1

Reference/KEGG Compound C00080
type: UnificationXref
id: C00080
db: KEGG Compound

ChemicalStructure/PW C001065
type: ChemicalStructure
structure format: SMILES
structure data: O=O

Reference/ChemSpider 730
type: UnificationXref
id: 730
db: ChemSpider

Protein/6127/Stoichiometry/10.0
type: Stoichiometry
physical entity: Pyrroline-5-carboxylate reductase
stoichiometric coefficient: 10.0

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

ChemicalStructure/PW C000095
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CCC(O)=O)C(O)=O

Reference/CAS 14265-44-2
type: UnificationXref
id: 14265-44-2
db: CAS

Reference/PubChem-compound 8231
type: UnificationXref
id: 8231
db: PubChem-compound

Reference/PubChem-compound 8230
type: UnificationXref
id: 8230
db: PubChem-compound

Reference/BioCyc PYRUVATE
type: UnificationXref
id: PYRUVATE
db: BioCyc

Reference/CAS 58-64-0
type: UnificationXref
id: 58-64-0
db: CAS

pyrroline-5-carboxylate reductase
type: Complex
component stoichiometry: Protein/6127 Cytosol/Stoichiometry/10.0
cellular location: Cytosol
component: Pyrroline-5-carboxylate reductase

Reference/KEGG Compound C04322
type: UnificationXref
id: C04322
db: KEGG Compound

Compound/PW C040034 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/BioCyc L-GLUTAMATE GAMMA-SEMIALDEHYDE
type: UnificationXref
id: L-GLUTAMATE_GAMMA-SEMIALDEHYDE
db: BioCyc

Bifunctional protein PutA
type: ProteinReference
xref: Reference/UniProt P09546
organism: Escherichia coli

Compound/PW C000106 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Proline
stoichiometric coefficient: 1.0

L-Glutamic γ-semialdehyde
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0002104
xref: Reference/PubChem-compound 193305
xref: Reference/CAS 496-92-4
xref: Reference/ChemSpider 167744
xref: Reference/KEGG Compound C01165
xref: Reference/BioCyc L-GLUTAMATE GAMMA-SEMIALDEHYDE
xref: Reference/ChEBI CHEBI 17232
chemical formula: C5H9NO3
molecular weight: 131.05824
structure: ChemicalStructure/PW C001415

Reference/PubChem-compound 22833512
type: UnificationXref
id: 22833512
db: PubChem-compound

ChemicalStructure/PW C000075
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)C=O

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

ChemicalStructure/PW C000078
type: ChemicalStructure
structure format: SMILES
structure data: NCC(O)=O

Reference/BioCyc NADH
type: UnificationXref
id: NADH
db: BioCyc

Reference/HMDB HMDB0002012
type: UnificationXref
id: HMDB0002012
db: HMDB

Reference/BioCyc NAD
type: UnificationXref
id: NAD
db: BioCyc

Reference/PubChem-compound 977
type: UnificationXref
id: 977
db: PubChem-compound

Reference/HMDB HMDB0059597
type: UnificationXref
id: HMDB0059597
db: HMDB

Compound/PW C001316 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Carbon dioxide
stoichiometric coefficient: 1.0

L-Glutamic acid
type: SmallMolecule
cellular location: Inner Membrane
entity reference: L-Glutamic acid

L-Proline
type: SmallMolecule
entity reference: L-Proline

Reference/SMPDB SMP0002095
type: UnificationXref
id: SMP0002095
db: SMPDB

Reference/ChemSpider 740
type: UnificationXref
id: 740
db: ChemSpider

Pyrroline-5-carboxylate reductase
type: Protein
entity reference: Pyrroline-5-carboxylate reductase

4-Hydroxyproline
type: SmallMoleculeReference
xref: Reference/ChemSpider 5605
xref: Reference/ChEBI CHEBI 18095
xref: Reference/HMDB HMDB0000725
xref: Reference/CAS 51-35-4
xref: Reference/PubChem-compound 5810
xref: Reference/KEGG Compound C01157
xref: Reference/BioCyc L-4-HYDROXY-PROLINE
chemical formula: C5H9NO3
molecular weight: 131.05824
structure: ChemicalStructure/PW C002643

ChemicalStructure/PW C001057
type: ChemicalStructure
structure format: SMILES
structure data: OC1CC(N=C1)C(O)=O

Adenosine diphosphate
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Adenosine diphosphate

Compound/PW C001104 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphate
stoichiometric coefficient: 1.0

Compound/PW C002643 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: 4-Hydroxyproline
stoichiometric coefficient: 1.0

Gamma-glutamyl phosphate reductase
type: ProteinReference
xref: Reference/UniProt P07004

Compound/PW C001415/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic γ-semialdehyde
stoichiometric coefficient: 1.0

Reference/ChemSpider 30572
type: UnificationXref
id: 30572
db: ChemSpider

Reference/ChemSpider 17215925
type: UnificationXref
id: 17215925
db: ChemSpider

ChemicalStructure/PW C001415
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CCC=O)C(O)=O

NADH
type: SmallMolecule
entity reference: NADH

1-Pyrroline-5-carboxylic acid
type: SmallMoleculeReference
xref: Reference/ChemSpider 1159
xref: Reference/HMDB HMDB0001301
xref: Reference/ChEBI CHEBI 1372
xref: Reference/CAS 2906-39-0
xref: Reference/BioCyc l-delta(1)-pyrroline 5-carboxylate
xref: Reference/KEGG Compound C04322
xref: Reference/PubChem-compound 1196
chemical formula: C5H7NO2
molecular weight: 113.047676
structure: ChemicalStructure/PW C001003

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Reference/PubChem-compound 6426709
type: UnificationXref
id: 6426709
db: PubChem-compound

Reference/TAXONOMY 83333
type: UnificationXref
id: 83333
db: TAXONOMY

Reference/ChemSpider 167893
type: UnificationXref
id: 167893
db: ChemSpider

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

Compound/PW C001065 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Oxygen
stoichiometric coefficient: 1.0

Prolylglycine
type: SmallMoleculeReference
xref: Reference/ChemSpider 4932143
xref: Reference/CAS 2578-57-6
xref: Reference/PubChem-compound 6426709
xref: Reference/HMDB HMDB0011178
xref: Reference/ChEBI CHEBI 61695
chemical formula: C7H12N2O3
molecular weight: 172.0848
structure: ChemicalStructure/PW C006813

Compound/PW C001034 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine diphosphate
stoichiometric coefficient: 1.0

Compound/PW C000414/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

ChemicalStructure/PW C002643
type: ChemicalStructure
structure format: SMILES
structure data: O[C@H]1CN[C@@H](C1)C(O)=O

Reference/PubChem-compound 51
type: UnificationXref
id: 51
db: PubChem-compound

Water
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Water

Pyruvic acid
type: SmallMolecule
entity reference: Pyruvic acid
name: SubPathwayInput

Reference/ChEBI CHEBI 15846
type: UnificationXref
id: CHEBI:15846
db: ChEBI

Reference/BioCyc ADP
type: UnificationXref
id: ADP
db: BioCyc

Pyruvic acid
type: SmallMoleculeReference
xref: Reference/PubChem-compound 1060
xref: Reference/HMDB HMDB0000243
xref: Reference/CAS 127-17-3
xref: Reference/BioCyc PYRUVATE
xref: Reference/KEGG Compound C00022
xref: Reference/ChEBI CHEBI 32816
xref: Reference/ChemSpider 1031
chemical formula: C3H4O3
molecular weight: 88.016045
structure: ChemicalStructure/PW C000164

Compound/PW C000106 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Proline
stoichiometric coefficient: 1.0

Glycine
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Glycine

Reference/CAS 53-59-8
type: UnificationXref
id: 53-59-8
db: CAS

Compound/PW C001057 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Pyrroline hydroxycarboxylic acid
stoichiometric coefficient: 1.0

Reference/ChemSpider 167744
type: UnificationXref
id: 167744
db: ChemSpider

Reference/PubChem-compound 1038
type: UnificationXref
id: 1038
db: PubChem-compound

Reference/KEGG Compound C03287
type: UnificationXref
id: C03287
db: KEGG Compound

glutamate / aspartate ABC transporter
type: Complex
cellular location: Inner Membrane
component: Glutamate/aspartate transport system permease protein gltK
component: Glutamate/aspartate transport ATP-binding protein gltL
component: Glutamate/aspartate periplasmic-binding protein
component: Glutamate/aspartate transport system permease protein gltJ

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Compound/PW C282129 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-erythro-4-Hydroxyglutamate
stoichiometric coefficient: 1.0

1-Pyrroline-5-carboxylic acid
type: SmallMolecule
entity reference: 1-Pyrroline-5-carboxylic acid

Reference/ChEBI CHEBI 18095
type: UnificationXref
id: CHEBI:18095
db: ChEBI

Reference/ChemSpider 274
type: UnificationXref
id: 274
db: ChemSpider

Oxoglutaric acid
type: SmallMoleculeReference
xref: Reference/CAS 328-50-7
xref: Reference/PubChem-compound 51
xref: Reference/BioCyc 2-KETOGLUTARATE
xref: Reference/HMDB HMDB0000208
xref: Reference/ChEBI CHEBI 30915
xref: Reference/KEGG Compound C00026
xref: Reference/ChemSpider 50
chemical formula: C5H6O5
molecular weight: 146.02153
structure: ChemicalStructure/PW C000134

Reference/ChEBI CHEBI 16908
type: UnificationXref
id: CHEBI:16908
db: ChEBI

Aspartate aminotransferase
type: ProteinReference
xref: Reference/UniProt P00509
organism: Escherichia coli

Phosphate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00009
xref: Reference/PubChem-compound 1061
xref: Reference/CAS 14265-44-2
xref: Reference/HMDB HMDB0001429
xref: Reference/BioCyc CPD-8587
xref: Reference/ChEBI CHEBI 18367
xref: Reference/ChemSpider 1032
chemical formula: H3O4P
molecular weight: 97.9769
structure: ChemicalStructure/PW C001104

Ubiquinone-1
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 16389
xref: Reference/ChemSpider 4307
xref: Reference/PubChem-compound 4462
xref: Reference/CAS 727-81-1
xref: Reference/HMDB HMDB0002012
xref: Reference/KEGG Compound C00399
chemical formula: C14H18O4
molecular weight: 250.12051
structure: ChemicalStructure/PW C001347

Ubiquinol-1
type: SmallMolecule
entity reference: Ubiquinol-1

NADPH
type: SmallMolecule
entity reference: NADPH

Reference/GENE ONTOLOGY GO 0070258
type: UnificationXref
id: GO:0070258
db: GENE ONTOLOGY

Glutamate 5-kinase
type: Protein
entity reference: Glutamate 5-kinase

NADP
type: SmallMolecule
entity reference: NADP

NADPH
type: SmallMoleculeReference
xref: Reference/CAS 53-57-6
xref: Reference/HMDB HMDB0000221
xref: Reference/PubChem-compound 22833512
xref: Reference/BioCyc NADPH
xref: Reference/ChemSpider 17215925
xref: Reference/KEGG Compound C00005
xref: Reference/ChEBI CHEBI 16474
chemical formula: C21H30N7O17P3
molecular weight: 745.0911
structure: ChemicalStructure/PW C000146

NADP
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00006
xref: Reference/CAS 53-59-8
xref: Reference/BioCyc NAD(P)
xref: Reference/HMDB HMDB0000217
xref: Reference/ChEBI CHEBI 18009
xref: Reference/PubChem-compound 5886
xref: Reference/ChemSpider 5675
chemical formula: C21H28N7O17P3
molecular weight: 743.07544
structure: ChemicalStructure/PW C000143

Reference/PathWhiz PW000827
type: UnificationXref
id: PW000827
db: PathWhiz

Reference/BioCyc NADPH
type: UnificationXref
id: NADPH
db: BioCyc

Reference/BioCyc GLY
type: UnificationXref
id: GLY
db: BioCyc

Reference/HMDB HMDB0000208
type: UnificationXref
id: HMDB0000208
db: HMDB

Carbon dioxide
type: SmallMolecule
cellular location: Cytosol
entity reference: Carbon dioxide

Reference/BioCyc CPD-6641
type: UnificationXref
id: CPD-6641
db: BioCyc

Reference/PubChem-compound 5810
type: UnificationXref
id: 5810
db: PubChem-compound

Reference/CAS 58-68-4
type: UnificationXref
id: 58-68-4
db: CAS

L-Glutamic acid
type: SmallMolecule
entity reference: L-Glutamic acid

Reference/HMDB HMDB0001301
type: UnificationXref
id: HMDB0001301
db: HMDB

ChemicalStructure/PW C001104
type: ChemicalStructure
structure format: SMILES
structure data: OP(O)(O)=O

Reference/KEGG Compound C04281
type: UnificationXref
id: C04281
db: KEGG Compound

Reference/HMDB HMDB0000217
type: UnificationXref
id: HMDB0000217
db: HMDB

ChemicalStructure/PW C000134
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)CCC(=O)C(O)=O

Reference/ChEBI CHEBI 30915
type: UnificationXref
id: CHEBI:30915
db: ChEBI

Reference/CAS 124-38-9
type: UnificationXref
id: 124-38-9
db: CAS

Reference/BioCyc l-delta(1)-pyrroline 5-carboxylate
type: UnificationXref
id: l-delta(1)-pyrroline_5-carboxylate
db: BioCyc

Glycine
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000123
xref: Reference/BioCyc GLY
xref: Reference/ChemSpider 730
xref: Reference/PubChem-compound 5257127
xref: Reference/CAS 56-40-6
xref: Reference/ChEBI CHEBI 15428
xref: Reference/KEGG Compound C00037
chemical formula: C2H5NO2
molecular weight: 75.03203
structure: ChemicalStructure/PW C000078

Hydrogen Ion
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Hydrogen Ion

Glyoxylic acid
type: SmallMoleculeReference
xref: Reference/BioCyc GLYOX
xref: Reference/ChemSpider 740
xref: Reference/CAS 298-12-4
xref: Reference/HMDB HMDB0000119
xref: Reference/PubChem-compound 760
xref: Reference/ChEBI CHEBI 16891
xref: Reference/KEGG Compound C00048
chemical formula: C2H2O3
molecular weight: 74.0004
structure: ChemicalStructure/PW C000075

Reference/UniProt P0A9L8
type: UnificationXref
id: P0A9L8
db: UniProt

Reference/HMDB HMDB0001429
type: UnificationXref
id: HMDB0001429
db: HMDB

Protein/107695 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Bifunctional protein PutA
stoichiometric coefficient: 2.0

Compound/PW C000721 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: NAD
stoichiometric coefficient: 1.0

ChemicalStructure/PW C001347
type: ChemicalStructure
structure format: SMILES
structure data: COC1=C(OC)C(=O)C(CC=C(C)C)=C(C)C1=O

Inner Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0070258

aspartate transaminase
type: Complex
component stoichiometry: Protein/5824 Cytosol/Stoichiometry/2.0
cellular location: Cytosol
component: Aspartate aminotransferase

ChemicalStructure/PW C000143
type: ChemicalStructure
structure format: SMILES

Reference/HMDB HMDB0000221
type: UnificationXref
id: HMDB0000221
db: HMDB

Reference/PubChem-compound 5257127
type: UnificationXref
id: 5257127
db: PubChem-compound

ChemicalStructure/PW C000146
type: ChemicalStructure
structure format: SMILES

Pyrroline hydroxycarboxylic acid
type: SmallMoleculeReference
xref: Reference/KEGG Compound C04281
xref: Reference/HMDB HMDB0001369
xref: Reference/BioCyc PYRROLINE-HYDROXY-CARBOXYLATE
xref: Reference/CAS 22573-88-2
xref: Reference/ChEBI CHEBI 27391
xref: Reference/ChemSpider 1030
xref: Reference/PubChem-compound 1059
chemical formula: C5H7NO3
molecular weight: 129.04259
structure: ChemicalStructure/PW C001057

Reference/BioCyc GLT
type: UnificationXref
id: GLT
db: BioCyc

Reference/CAS 53-57-6
type: UnificationXref
id: 53-57-6
db: CAS

Oxoglutaric acid
type: SmallMolecule
cellular location: Cytosol
entity reference: Oxoglutaric acid

Reference/ChEBI CHEBI 15741
type: UnificationXref
id: CHEBI:15741
db: ChEBI

Compound/PW C040034 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Compound/PW C000174 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Succinic acid
stoichiometric coefficient: 1.0

ChemicalStructure/PW C000106
type: ChemicalStructure
structure format: SMILES
structure data: OC(=O)[C@@H]1CCCN1

ChemicalStructure/PW C001316
type: ChemicalStructure
structure format: SMILES
structure data: O=C=O

Bifunctional protein putA
type: ProteinReference
xref: Reference/UniProt P09546

Reference/PubChem-compound 1110
type: UnificationXref
id: 1110
db: PubChem-compound

Glyoxylic acid
type: SmallMolecule
entity reference: Glyoxylic acid
name: SubPathwayInput

NADH
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001487
xref: Reference/CAS 58-68-4
xref: Reference/PubChem-compound 439153
xref: Reference/BioCyc NADH
xref: Reference/ChemSpider 388299
xref: Reference/KEGG Compound C00004
xref: Reference/ChEBI CHEBI 16908
chemical formula: C21H29N7O14P2
molecular weight: 665.12476
structure: ChemicalStructure/PW C001144

Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

Reaction2722
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001415/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000095/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000721/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001144/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/2.0
right: NADH
right: L-Glutamic acid
right: Hydrogen Ion
spontaneous: false
left: Water
left: L-Glutamic γ-semialdehyde
left: NAD
name: L-Glutamic γ-semialdehyde + NAD + Water → 2 Hydrogen Ion + L-Glutamic acid + NADH
conversion direction: LEFT_TO_RIGHT

1.5.5.2
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001347/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001003/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000106/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
participant stoichiometry: Compound/PW C042948/Stoichiometry/1.0
right: 1-Pyrroline-5-carboxylic acid
right: Hydrogen Ion
right: Ubiquinol-1
spontaneous: false
EC number: 1.5.5.2
left: Ubiquinone-1
left: L-Proline
name: L-Proline + Ubiquinone-1 → 1-Pyrroline-5-carboxylic acid + Hydrogen Ion + Ubiquinol-1
conversion direction: LEFT_TO_RIGHT

Reaction2721
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001420/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001415/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001003/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
right: L-Glutamic γ-semialdehyde
spontaneous: true
left: Water
left: 1-Pyrroline-5-carboxylic acid
left: Hydrogen Ion
name: 1-Pyrroline-5-carboxylic acid + Hydrogen Ion + Water → L-Glutamic γ-semialdehyde
conversion direction: LEFT_TO_RIGHT

Compound/PW C000095 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic acid
stoichiometric coefficient: 1.0

Succinic acid
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000254
xref: Reference/CAS 110-15-6
xref: Reference/ChEBI CHEBI 15741
xref: Reference/KEGG Compound C00042
xref: Reference/ChemSpider 1078
xref: Reference/PubChem-compound 1110
xref: Reference/BioCyc SUC
chemical formula: C4H6O4
molecular weight: 118.02661
structure: ChemicalStructure/PW C000174

1.5.1.2
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000146/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001003/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000106/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
right: NADP
right: L-Proline
spontaneous: false
EC number: 1.5.1.2
left: 1-Pyrroline-5-carboxylic acid
left: Hydrogen Ion
left: NADPH
name: 1-Pyrroline-5-carboxylic acid + Hydrogen Ion + NADPH → L-Proline + NADP
conversion direction: LEFT_TO_RIGHT

Protein/113962 Cytoplasm/Stoichiometry/6.0
type: Stoichiometry
physical entity: Peptidase B
stoichiometric coefficient: 6.0

Reaction2717
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001415/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001003/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
right: Water
right: 1-Pyrroline-5-carboxylic acid
right: Hydrogen Ion
spontaneous: false
left: L-Glutamic γ-semialdehyde
name: L-Glutamic γ-semialdehyde → 1-Pyrroline-5-carboxylic acid + Hydrogen Ion + Water
conversion direction: LEFT_TO_RIGHT

1.2.1.41
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000146/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001415/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000951/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001104/Stoichiometry/1.0
right: NADP
right: L-Glutamic γ-semialdehyde
right: Phosphate
spontaneous: false
EC number: 1.2.1.41
left: Hydrogen Ion
left: L-Glutamic acid 5-phosphate
left: NADPH
name: Hydrogen Ion + L-Glutamic acid 5-phosphate + NADPH → L-Glutamic γ-semialdehyde + NADP + Phosphate
conversion direction: LEFT_TO_RIGHT

Compound/PW C282130 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: (4R)-4-Hydroxy-2-oxoglutarate
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0000119
type: UnificationXref
id: HMDB0000119
db: HMDB

Peptidase B
type: ProteinReference
xref: Reference/UniProt P37095
organism: BiologicalState235

Peptidase B
type: Protein
cellular location: Cytoplasm
entity reference: Peptidase B

Reference/HMDB HMDB0000123
type: UnificationXref
id: HMDB0000123
db: HMDB

Adenosine diphosphate
type: SmallMolecule
entity reference: Adenosine diphosphate

Reference/HMDB HMDB0000243
type: UnificationXref
id: HMDB0000243
db: HMDB

Adenosine diphosphate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00008
xref: Reference/PubChem-compound 6022
xref: Reference/ChemSpider 5800
xref: Reference/CAS 58-64-0
xref: Reference/BioCyc ADP
xref: Reference/ChEBI CHEBI 16761
xref: Reference/HMDB HMDB0001341
chemical formula: C10H15N5O10P2
molecular weight: 427.02942
structure: ChemicalStructure/PW C001034

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

Reference/CAS 147-85-3
type: UnificationXref
id: 147-85-3
db: CAS

Compound/PW C000951/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic acid 5-phosphate
stoichiometric coefficient: 1.0

(4R)-4-Hydroxy-2-oxoglutarate
type: SmallMolecule
cellular location: Cytosol
entity reference: (4R)-4-Hydroxy-2-oxoglutarate

Compound/PW C000721/Stoichiometry/1.0
type: Stoichiometry
physical entity: NAD
stoichiometric coefficient: 1.0

Adenosine triphosphate
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Adenosine triphosphate

Reference/BioCyc CPD-8587
type: UnificationXref
id: CPD-8587
db: BioCyc

ChemicalStructure/PW C000721
type: ChemicalStructure
structure format: SMILES

Reference/ChEBI CHEBI 197035
type: UnificationXref
id: CHEBI: 197035
db: ChEBI

Reference/ChEBI CHEBI 16891
type: UnificationXref
id: CHEBI:16891
db: ChEBI

Reference/ChemSpider 5682
type: UnificationXref
id: 5682
db: ChemSpider

ReactionCatalysis2030481
type: Catalysis
controller: Peptidase B
controlled: 3.4.11.23
control type: ACTIVATION

Pyrroline hydroxycarboxylic acid
type: SmallMolecule
cellular location: Cytosol
entity reference: Pyrroline hydroxycarboxylic acid

Reference/ChEBI CHEBI 61695
type: UnificationXref
id: CHEBI:61695
db: ChEBI

Phosphate
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Phosphate

Reference/UniProt P37902
type: UnificationXref
id: P37902
db: UniProt

Compound/PW C000414 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Reference/ChemSpider 5675
type: UnificationXref
id: 5675
db: ChemSpider

Reference/ChemSpider 1078
type: UnificationXref
id: 1078
db: ChemSpider

Reference/ChEBI CHEBI 1372
type: UnificationXref
id: CHEBI:1372
db: ChEBI

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

Compound/PW C001420 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 2.0

Water
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Water

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

Reference/CAS 22573-88-2
type: UnificationXref
id: 22573-88-2
db: CAS

Escherichia coli
type: BioSource
xref: Reference/TAXONOMY 562

Reference/ChemSpider 388299
type: UnificationXref
id: 388299
db: ChemSpider

Oxygen
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001377
xref: Reference/ChemSpider 952
xref: Reference/KEGG Compound C00007
xref: Reference/ChEBI CHEBI 15379
xref: Reference/PubChem-compound 977
xref: Reference/BioCyc CPD-6641
xref: Reference/CAS 7782-44-7
chemical formula: O2
molecular weight: 31.98983
structure: ChemicalStructure/PW C001065

Reference/CAS 127-17-3
type: UnificationXref
id: 127-17-3
db: CAS

Reference/BioCyc PYRROLINE-HYDROXY-CARBOXYLATE
type: UnificationXref
id: PYRROLINE-HYDROXY-CARBOXYLATE
db: BioCyc

BiologicalState2028
type: BioSource
xref: Reference/TAXONOMY 511145
name: Escherichia coli str. K-12 substr. MG1655

L-Glutamic acid
type: SmallMoleculeReference
xref: Reference/PubChem-compound 33032
xref: Reference/ChEBI CHEBI 16015
xref: Reference/ChemSpider 30572
xref: Reference/HMDB HMDB0000148
xref: Reference/CAS 56-86-0
xref: Reference/BioCyc GLT
xref: Reference/KEGG Compound C00025
chemical formula: C5H9NO4
molecular weight: 147.05316
structure: ChemicalStructure/PW C000095

Water
type: SmallMolecule
cellular location: Cytosol
entity reference: Water

Reference/PathWhiz PW000794
type: UnificationXref
id: PW000794
db: PathWhiz

Reference/PubChem-compound 33032
type: UnificationXref
id: 33032
db: PubChem-compound

Reference/ChEBI CHEBI 16526
type: UnificationXref
id: CHEBI:16526
db: ChEBI

Compound/PW C000095 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Glutamic acid
stoichiometric coefficient: 1.0

Unknown
type: Protein
cellular location: Cytoplasm
entity reference: Unknown

ChemicalStructure/PW C000951
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CCC(=O)OP(O)(O)=O)C(O)=O

Compound/PW C001144/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADH
stoichiometric coefficient: 1.0

Gamma-glutamyl phosphate reductase
type: Protein
entity reference: Gamma-glutamyl phosphate reductase

Reference/ChEBI CHEBI 16761
type: UnificationXref
id: CHEBI:16761
db: ChEBI

Reference/CAS 7782-44-7
type: UnificationXref
id: 7782-44-7
db: CAS

Succinic acid
type: SmallMolecule
cellular location: Cytosol
entity reference: Succinic acid

Reference/ChEBI CHEBI 15428
type: UnificationXref
id: CHEBI:15428
db: ChEBI

Reference/BioCyc 2-KETOGLUTARATE
type: UnificationXref
id: 2-KETOGLUTARATE
db: BioCyc

Reference/HMDB HMDB0000725
type: UnificationXref
id: HMDB0000725
db: HMDB

Ubiquinol-1
type: SmallMoleculeReference
xref: Reference/PubChem-compound 447920
chemical formula: C14H20O4
molecular weight: 252.13615
structure: ChemicalStructure/PW C042948

Reference/PubChem-compound 1059
type: UnificationXref
id: 1059
db: PubChem-compound

Compound/PW C001104/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphate
stoichiometric coefficient: 1.0

Reference/PubChem-compound 5893
type: UnificationXref
id: 5893
db: PubChem-compound

Reference/CAS 110-15-6
type: UnificationXref
id: 110-15-6
db: CAS

Reaction1960070
type: BiochemicalReaction
participant stoichiometry: Compound/PW C282130 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C282129 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000134 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000095 Cytosol/Stoichiometry/1.0
right: L-Glutamic acid
right: (4R)-4-Hydroxy-2-oxoglutarate
spontaneous: false
left: Oxoglutaric acid
left: L-erythro-4-Hydroxyglutamate
name: L-erythro-4-Hydroxyglutamate + Oxoglutaric acid → (4R)-4-Hydroxy-2-oxoglutarate + L-Glutamic acid
conversion direction: LEFT_TO_RIGHT

Reaction1960072
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000075 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C282130 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000164 Cytoplasm/Stoichiometry/1.0
right: Pyruvic acid
right: Glyoxylic acid
spontaneous: false
left: (4R)-4-Hydroxy-2-oxoglutarate
name: (4R)-4-Hydroxy-2-oxoglutarate → Glyoxylic acid + Pyruvic acid
conversion direction: LEFT_TO_RIGHT

Reference/TAXONOMY 511145
type: UnificationXref
id: 511145
db: TAXONOMY

Reference/ChEBI CHEBI 32816
type: UnificationXref
id: CHEBI:32816
db: ChEBI

Reference/ChEBI CHEBI 17798
type: UnificationXref
id: CHEBI:17798
db: ChEBI

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Reference/ChEBI CHEBI 15379
type: UnificationXref
id: CHEBI:15379
db: ChEBI

Reference/ChEBI CHEBI 15378
type: UnificationXref
id: CHEBI:15378
db: ChEBI

ChemicalStructure/PW C040034
type: ChemicalStructure
structure format: SMILES
structure data: [H+]

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Reference/PubChem-compound 5886
type: UnificationXref
id: 5886
db: PubChem-compound

Reference/CAS 51-35-4
type: UnificationXref
id: 51-35-4
db: CAS

Compound/PW C000078 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Glycine
stoichiometric coefficient: 1.0

ReactionCatalysis169197
type: Catalysis
controller: γ-glutamyl kinase
controlled: Reaction179292
control type: ACTIVATION

Reference/BioCyc L-4-HYDROXY-PROLINE
type: UnificationXref
id: L-4-HYDROXY-PROLINE
db: BioCyc

Oxygen
type: SmallMolecule
cellular location: Cytosol
entity reference: Oxygen

Compound/PW C001420/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

1.5.5.2
type: BiochemicalReaction
participant stoichiometry: Compound/PW C282129 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001057 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000721 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/2.0
participant stoichiometry: Compound/PW C001144 Cytosol/Stoichiometry/1.0
right: Hydrogen Ion
right: L-erythro-4-Hydroxyglutamate
right: NADH
spontaneous: false
EC number: 1.5.5.2
left: NAD
left: Pyrroline hydroxycarboxylic acid
left: Water
name: NAD + Pyrroline hydroxycarboxylic acid + 2 Water → Hydrogen Ion + L-erythro-4-Hydroxyglutamate + NADH
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 280
type: UnificationXref
id: 280
db: PubChem-compound

Reaction1960065
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000106 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000134 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000174 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001316 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C002643 Cytosol/Stoichiometry/1.0
right: Succinic acid
right: Carbon dioxide
right: 4-Hydroxyproline
spontaneous: false
left: Oxygen
left: Oxoglutaric acid
left: L-Proline
name: L-Proline + Oxoglutaric acid + Oxygen → 4-Hydroxyproline + Carbon dioxide + Succinic acid
conversion direction: LEFT_TO_RIGHT

Reaction1960066
type: BiochemicalReaction
participant stoichiometry: Compound/PW C040034 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001057 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000721 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001144 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C002643 Cytosol/Stoichiometry/1.0
right: Hydrogen Ion
right: Pyrroline hydroxycarboxylic acid
right: NADH
spontaneous: false
left: NAD
left: 4-Hydroxyproline
name: 4-Hydroxyproline + NAD → Hydrogen Ion + NADH + Pyrroline hydroxycarboxylic acid
conversion direction: LEFT_TO_RIGHT

Reference/ChemSpider 4932143
type: UnificationXref
id: 4932143
db: ChemSpider

Reference/UniProt P09546
type: UnificationXref
id: P09546
db: UniProt

Reference/PubChem-compound 4462
type: UnificationXref
id: 4462
db: PubChem-compound

Reference/CAS 2578-57-6
type: UnificationXref
id: 2578-57-6
db: CAS

Reference/UniProt P07004
type: UnificationXref
id: P07004
db: UniProt

Reference/PubChem-compound 1196
type: UnificationXref
id: 1196
db: PubChem-compound

Unknown
type: ProteinReference
organism: Escherichia coli

Pyrroline-5-carboxylate reductase
type: Protein
cellular location: Cytosol
entity reference: Pyrroline-5-carboxylate reductase

Reference/CAS 727-81-1
type: UnificationXref
id: 727-81-1
db: CAS

Compound/PW C040034/Stoichiometry/2.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 2.0

Reference/PubChem-compound 1060
type: UnificationXref
id: 1060
db: PubChem-compound

Reference/PubChem-compound 1061
type: UnificationXref
id: 1061
db: PubChem-compound

Reference/UniProt P0A7B5
type: UnificationXref
id: P0A7B5
db: UniProt

Reference/HMDB HMDB0001967
type: UnificationXref
id: HMDB0001967
db: HMDB

Compound/PW C001420 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Compound/PW C282130 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: (4R)-4-Hydroxy-2-oxoglutarate
stoichiometric coefficient: 1.0

(4R)-4-Hydroxy-2-oxoglutarate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: (4R)-4-Hydroxy-2-oxoglutarate

Reference/UniProt P00509
type: UnificationXref
id: P00509
db: UniProt

Reference/CAS 2906-39-0
type: UnificationXref
id: 2906-39-0
db: CAS

L-Proline
type: SmallMolecule
cellular location: Cytoplasm
entity reference: L-Proline

Reference/PubChem-compound 193475
type: UnificationXref
id: 193475
db: PubChem-compound