Acetyl-CoA + Oxalacetic acid + Water → Citric acid + Coenzyme A + Hydrogen Ion
Citric acid → Water + cis-Aconitic acid
cis-Aconitic acid → trans-Aconitic acid
Water + cis-Aconitic acid ↔ Isocitric acid
Glyoxylic acid + Pyruvic acid → 4-Hydroxy-2-oxoglutaric acid
4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
Acetyl-CoA + Glyoxylic acid + Water → Coenzyme A + Hydrogen Ion + L-Malic acid
L-Malic acid + NAD → Hydrogen Ion + NADH + Oxalacetic acid
Isocitric acid ↔ Glyoxylic acid + Succinic acid
4-Hydroxy-2-oxoglutaric acid + L-Glutamic acid ↔ 4-Hydroxy-L-glutamic acid + Oxoglutaric acid
Citrate hydro-lyase (cis-aconitate-forming)
Hydroxypropanedioic acid inhibits isocitrate lyase