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Pathway Description
Vitamin B1/Thiamine Metabolism
Saccharomyces cerevisiae
Metabolic Pathway
The biosynthesis of thiamine begins with pyrithiamine reacting with thiaminase 2 resulting in the release of 4-Amino-5-hydroxymethyl-2-methylpyrimidine. The latter compound reacts with a hydroxymethylpyrimidine/phosphomethylpyrimidine kinase resulting in the release of 4-amino-2-methyl-5-phosphomethylpyrimidine. The latter compound reacts with a hydroxymethylpyrimidine/phosphomethylpyrimidine kinase resulting in the release of 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate. The latter compound reacts with 4-methyl-5-(2-phosphonooxyethyl)thiazole, a product of oxythiamine metabolism, through a Thiamine biosynthetic bifunctional enzyme resultin in the release of a Thiamine monophosphate. The latter compound is phosphatased through a acid phosphatase complex resulting in the release of thiamine. The latter compound is phosphorylated through a thiamin pyrophosphokinase resulting in the release of thiamine pyrophosphate.
References
Vitamin B1/Thiamine Metabolism References
French JB, Begley TP, Ealick SE: Structure of trifunctional THI20 from yeast. Acta Crystallogr D Biol Crystallogr. 2011 Sep;67(Pt 9):784-91. doi: 10.1107/S0907444911024814. Epub 2011 Aug 9.
Pubmed: 21904031
Haas AL, Laun NP, Begley TP: Thi20, a remarkable enzyme from Saccharomyces cerevisiae with dual thiamin biosynthetic and degradation activities. Bioorg Chem. 2005 Aug;33(4):338-44. doi: 10.1016/j.bioorg.2005.04.001.
Pubmed: 15967475
Iwashima A, Nishino H, Nose Y: Carrier-mediated transport of thiamine in baker's yeast. Biochim Biophys Acta. 1973 Dec 13;330(2):222-34.
Pubmed: 4591128
Iwashima A, Yoshioka K, Nishimura H, Nosaka K: Reversal of pyrithiamine-induced growth inhibition of Saccharomyces cerevisiae by oxythiamine. Experientia. 1984 Jun 15;40(6):582-3.
Pubmed: 6373358
Iwashima A, Nosaka K, Nishimura H, Kimura Y: Some properties of a Saccharomyces cerevisiae mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole. J Gen Microbiol. 1986 Jun;132(6):1541-6. doi: 10.1099/00221287-132-6-1541.
Pubmed: 3027234
Iwashima A, Kawasaki Y, Kimura Y: Transport of 2-methyl-4-amino-5-hydroxymethylpyrimidine in Saccharomyces cerevisiae. Biochim Biophys Acta. 1990 Feb 28;1022(2):211-4.
Pubmed: 2407290
Kimura Y, Iwashima A: Occurrence of thiaminase II in Saccharomyces cerevisiae. Experientia. 1987 Aug 15;43(8):888-90.
Pubmed: 3305065
Kowalska E, Kozik A: The genes and enzymes involved in the biosynthesis of thiamin and thiamin diphosphate in yeasts. Cell Mol Biol Lett. 2008;13(2):271-82. doi: 10.2478/s11658-007-0055-5. Epub 2008 Apr 10.
Pubmed: 18161008
Onozuka M, Konno H, Kawasaki Y, Akaji K, Nosaka K: Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiae. FEMS Yeast Res. 2008 Mar;8(2):266-75. doi: 10.1111/j.1567-1364.2007.00333.x. Epub 2007 Nov 19.
Pubmed: 18028398
Baker LJ, Dorocke JA, Harris RA, Timm DE: The crystal structure of yeast thiamin pyrophosphokinase. Structure. 2001 Jun;9(6):539-46.
Pubmed: 11435118
Kawasaki Y: Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway. J Bacteriol. 1993 Aug;175(16):5153-8.
Pubmed: 8394314
Nosaka K, Nishimura H, Kawasaki Y, Tsujihara T, Iwashima A: Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae. J Biol Chem. 1994 Dec 2;269(48):30510-6.
Pubmed: 7982968
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