Compound/PW C040034 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/ChemSpider 21864867
type: UnificationXref
id: 21864867
db: ChemSpider

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Reference/HMDB HMDB0012196
type: UnificationXref
id: HMDB0012196
db: HMDB

Reference/PubChem-compound 13475125
type: UnificationXref
id: 13475125
db: PubChem-compound

Cytochrome P450 90B1
type: Protein
cellular location: Cell Membrane
entity reference: Cytochrome P450 90B1

Reference/KEGG Compound C15802
type: UnificationXref
id: C15802
db: KEGG Compound

Compound/PW C105122 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxoteasterone
stoichiometric coefficient: 1.0

Compound/PW C001065/Stoichiometry/1.0
type: Stoichiometry
physical entity: Oxygen
stoichiometric coefficient: 1.0

Cytochrome P450 85A2
type: Protein
entity reference: Cytochrome P450 85A2

Reference/PubChem-compound 23724587
type: UnificationXref
id: 23724587
db: PubChem-compound

Compound/PW C040034/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 20747
type: UnificationXref
id: CHEBI:20747
db: ChEBI

Cytochrome P450 90B1
type: Protein
cellular location: Membrane
entity reference: Cytochrome P450 90B1

Steroid 5-alpha-reductase DET2
type: Protein
entity reference: Steroid 5-alpha-reductase DET2

Reference/HMDB HMDB0041527
type: UnificationXref
id: HMDB0041527
db: HMDB

ChemicalStructure/PW C015039
type: ChemicalStructure
structure format: SMILES

(22α)-hydroxy-campest-4-en-3-one
type: SmallMolecule
cellular location: Membrane
entity reference: (22α)-hydroxy-campest-4-en-3-one

NADPH
type: SmallMolecule
cellular location: Membrane
entity reference: NADPH

Compound/PW C104784 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: FMN
stoichiometric coefficient: 1.0

Reference/ChemSpider 21142762
type: UnificationXref
id: 21142762
db: ChemSpider

Reference/PubChem-compound 15542699
type: UnificationXref
id: 15542699
db: PubChem-compound

Reference/PubChem-compound 13475117
type: UnificationXref
id: 13475117
db: PubChem-compound

Compound/PW C105111 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22R,23R)-22,23-Dihydroxycampesterol
stoichiometric coefficient: 1.0

Reference/CAS 87734-68-7
type: UnificationXref
id: 87734-68-7
db: CAS

3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
type: Protein
entity reference: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1

Reference/KEGG Compound C01789
type: UnificationXref
id: C01789
db: KEGG Compound

Reference/ChEBI CHEBI 18534
type: UnificationXref
id: CHEBI:18534
db: ChEBI

Steroid Biosynthesis
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway112412
pathway component: SubPathwayInteraction112412
xref: Reference/PathWhiz PW122531
xref: Reference/SMPDB SMP0121236
name: SubPathway

Compound/PW C001065 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Oxygen
stoichiometric coefficient: 1.0

Brassinosteroid Synthesis
type: Pathway
pathway order: PathwaySubPathway/112412
pathway order: PathwayEnzymeReaction/2169135
pathway order: PathwayEnzymeReaction/2169234
pathway order: PathwayEnzymeReaction/2169233
pathway order: PathwayEnzymeReaction/2169133
pathway order: PathwayEnzymeReaction/2169232
pathway order: PathwayEnzymeReaction/2169496
pathway order: PathwayEnzymeReaction/2169231
pathway order: PathwayEnzymeReaction/2169495
pathway order: PathwayEnzymeReaction/2169237
pathway order: PathwayEnzymeReaction/2169236
pathway order: PathwayEnzymeReaction/2169235
pathway order: PathwayEnzymeReaction/2169490
pathway order: PathwayEnzymeReaction/2429569
pathway order: PathwayEnzymeReaction/2169494
pathway order: PathwayEnzymeReaction/2169492
pathway order: PathwayEnzymeReaction/2169491
pathway order: PathwayEnzymeReaction/2438319
pathway order: PathwayEnzymeReaction/2438318
pathway order: PathwayEnzymeReaction/2438317
pathway order: PathwayEnzymeReaction/2438321
pathway order: PathwayEnzymeReaction/2438320
pathway order: PathwayEnzymeReaction/2449717
pathway order: PathwayEnzymeReaction/2449718
pathway order: PathwayEnzymeReaction/2169487
pathway order: PathwayEnzymeReaction/2429694
pathway order: PathwayEnzymeReaction/2449715
pathway order: PathwayEnzymeReaction/2169486
pathway order: PathwayEnzymeReaction/2449716
pathway order: PathwayEnzymeReaction/2169485
pathway order: PathwayEnzymeReaction/2449713
pathway order: PathwayEnzymeReaction/2169484
pathway order: PathwayEnzymeReaction/2449714
pathway order: PathwayEnzymeReaction/2429335
pathway order: PathwayEnzymeReaction/2449712
pathway order: PathwayEnzymeReaction/2169489
pathway order: PathwayEnzymeReaction/2169488
pathway order: PathwayEnzymeReaction/2169481
pathway order: PathwayEnzymeReaction/2429957
pathway order: PathwayEnzymeReaction/2438148
pathway component: ReactionCatalysis200255
pathway component: ReactionCatalysis200256
pathway component: ReactionCatalysis200257
pathway component: Reaction210596
pathway component: ReactionCatalysis199665
pathway component: 1.14.-.-
pathway component: ReactionCatalysis201039
pathway component: Reaction210040
pathway component: Reaction210009
pathway component: ReactionCatalysis201035
pathway component: ReactionCatalysis201036
pathway component: ReactionCatalysis201037
pathway component: ReactionCatalysis201038
pathway component: ReactionCatalysis201033
pathway component: ReactionCatalysis201034
pathway component: ReactionCatalysis199656
pathway component: Reaction210609
pathway component: Steroid Biosynthesis
pathway component: Reaction187306
pathway component: Reaction210018
pathway component: Reaction210612
pathway component: Reaction210611
pathway component: Reaction210610
pathway component: Reaction211389
pathway component: Reaction187342
pathway component: Reaction187343
pathway component: 1.14.14.147
pathway component: 1.3.1.22
pathway component: Reaction187302
pathway component: ReactionCatalysis177059
pathway component: Reaction187346
pathway component: Reaction187347
pathway component: Reaction187304
pathway component: Reaction187348
pathway component: Reaction187305
pathway component: Reaction211390

Reference/ChEBI CHEBI 16474
type: UnificationXref
id: CHEBI:16474
db: ChEBI

Hydrogen Ion
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: Hydrogen Ion

Reference/ChemSpider 952
type: UnificationXref
id: 952
db: ChemSpider

ChemicalStructure/PW C108364
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C108363
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C108362
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C007746
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C108361
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C108366
type: ChemicalStructure
structure format: SMILES

Reference/UniProt Q94IW5
type: UnificationXref
id: Q94IW5
db: UniProt

Compound/PW C015039/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxocastasterone
stoichiometric coefficient: 1.0

ChemicalStructure/PW C108365
type: ChemicalStructure
structure format: SMILES

Castasterone
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: Castasterone

ChemicalStructure/PW C015136
type: ChemicalStructure
structure format: SMILES

Reference/ChemSpider 1010
type: UnificationXref
id: 1010
db: ChemSpider

3-Dehydroteasterone
type: SmallMolecule
entity reference: 3-Dehydroteasterone

Reference/UniProt O64989
type: UnificationXref
id: O64989
db: UniProt

ReactionCatalysis177026
type: Catalysis
controller: Steroid 5-alpha-reductase DET2
controlled: Reaction187306
control type: ACTIVATION

ReactionCatalysis177022
type: Catalysis
controller: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
controlled: 1.14.14.147
control type: ACTIVATION

Reference/CAS 5666-16-0
type: UnificationXref
id: 5666-16-0
db: CAS

ReactionCatalysis177023
type: Catalysis
controller: Cytochrome P450 90A1
controlled: 1.14.-.-
control type: ACTIVATION

ReactionCatalysis177024
type: Catalysis
controller: Steroid 5-alpha-reductase DET2
controlled: 1.3.1.22
control type: ACTIVATION

Compound/PW C022318/Stoichiometry/1.0
type: Stoichiometry
physical entity: 3-Dehydroteasterone
stoichiometric coefficient: 1.0

ReactionCatalysis177025
type: Catalysis
controller: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
controlled: 1.14.14.147
control type: ACTIVATION

ReactionCatalysis177021
type: Catalysis
controller: Cytochrome P450 90B1
controlled: 1.14.-.-
control type: ACTIVATION

Compound/PW C000146/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADPH
stoichiometric coefficient: 1.0

Hydrogen Ion
type: SmallMolecule
entity reference: Hydrogen Ion

Reference/UniProt Q8L7B5
type: UnificationXref
id: Q8L7B5
db: UniProt

ReactionCatalysis200255
type: Catalysis
controller: Cytochrome P450 90B1
controlled: Reaction210610
control type: ACTIVATION

Campestanol
type: SmallMolecule
entity reference: Campestanol

ReactionCatalysis200256
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction210612
control type: ACTIVATION

6-Deoxoteasterone
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: 6-Deoxoteasterone

ReactionCatalysis200257
type: Catalysis
controller: Cytochrome P450 85A2
controlled: 1.14.-.-
control type: ACTIVATION

Compound/PW C000891 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: FMNH2
stoichiometric coefficient: 1.0

Reference/PubChem-compound 25200612
type: UnificationXref
id: 25200612
db: PubChem-compound

FMNH2
type: SmallMolecule
entity reference: FMNH2

3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
type: Protein
cellular location: Endoplasmic Reticulum Membrane
entity reference: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1

Compound/PW C040034 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00007
type: UnificationXref
id: C00007
db: KEGG Compound

Reference/KEGG Compound C00006
type: UnificationXref
id: C00006
db: KEGG Compound

Reference/BioCyc CPD-3943
type: UnificationXref
id: CPD-3943
db: BioCyc

Reaction210040
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105116/Stoichiometry/1.0
participant stoichiometry: Compound/PW C108362/Stoichiometry/1.0
right: 3-Dehydro-6-deoxo-teasterone
spontaneous: false
left: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
name: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one → 3-Dehydro-6-deoxo-teasterone
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 18009
type: UnificationXref
id: CHEBI:18009
db: ChEBI

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Campesterol
type: SmallMoleculeReference
xref: Reference/KEGG Compound C01789
xref: Reference/BioCyc CPD-3943
xref: Reference/ChemSpider 4446730
xref: Reference/ChEBI CHEBI 19809
xref: Reference/HMDB HMDB0002869
xref: Reference/CAS 4651-51-8
xref: Reference/PubChem-compound 5283637
chemical formula: C28H48O
molecular weight: 400.3705
structure: ChemicalStructure/PW C001721

Reference/KEGG Compound C00005
type: UnificationXref
id: C00005
db: KEGG Compound

Reference/CAS 474-60-2
type: UnificationXref
id: 474-60-2
db: CAS

Reference/BioCyc CPD-3946
type: UnificationXref
id: CPD-3946
db: BioCyc

Oxygen
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: Oxygen

Reference/BioCyc CPD-3945
type: UnificationXref
id: CPD-3945
db: BioCyc

Compound/PW C000891 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: FMNH2
stoichiometric coefficient: 1.0

22alpha-Hydroxycampesterol
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: 22alpha-Hydroxycampesterol

Campesterol
type: SmallMolecule
entity reference: Campesterol

Reaction211389
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108363 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C015469 Mitochondrial Matrix/Stoichiometry/1.0
right: Castasterone
spontaneous: false
left: 2-Deoxycastasterone
name: 2-Deoxycastasterone → Castasterone
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 25201842
type: UnificationXref
id: 25201842
db: PubChem-compound

Reference/BioCyc NAD(P)
type: UnificationXref
id: NAD(P)
db: BioCyc

ReactionCatalysis177059
type: Catalysis
controller: Cytochrome P450 90B1
controlled: Reaction187342
control type: ACTIVATION

Cytochrome P450 734A1
type: Protein
cellular location: Membrane
entity reference: Cytochrome P450 734A1

Reaction211390
type: BiochemicalReaction
participant stoichiometry: Compound/PW C015039 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105113 Mitochondrial Matrix/Stoichiometry/1.0
right: 6-Deoxocastasterone
spontaneous: false
left: 6-Deoxotyphasterol
name: 6-Deoxotyphasterol → 6-Deoxocastasterone
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis177056
type: Catalysis
controller: Cytochrome P450 90B1
controlled: Reaction187334
control type: ACTIVATION

ReactionCatalysis177058
type: Catalysis
controller: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
controlled: Reaction187337
control type: ACTIVATION

Reference/ChEBI CHEBI 16048
type: UnificationXref
id: CHEBI:16048
db: ChEBI

Hydrogen Ion
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00080
xref: Reference/ChEBI CHEBI 15378
xref: Reference/HMDB HMDB0059597
xref: Reference/PubChem-compound 1038
xref: Reference/ChemSpider 1010
chemical formula: H
molecular weight: 1.007825
structure: ChemicalStructure/PW C040034

ReactionCatalysis177060
type: Catalysis
controller: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
controlled: 1.14.14.147
control type: ACTIVATION

Reaction211395
type: BiochemicalReaction
participant stoichiometry: Compound/PW C015136 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C108366 Membrane/Stoichiometry/1.0
right: 26-Hydroxybrassinolide
spontaneous: false
left: Brassinolide
name: Brassinolide → 26-Hydroxybrassinolide
conversion direction: LEFT_TO_RIGHT

Reaction211394
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108363 Inner Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C015136 Inner Membrane/Stoichiometry/1.0
right: Brassinolide
spontaneous: false
left: Castasterone
name: Castasterone → Brassinolide
conversion direction: LEFT_TO_RIGHT

1.14.-.-
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108365 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C108363 Membrane/Stoichiometry/1.0
right: 26-Hydroxycastasterone
spontaneous: false
EC number: 1.14.-.-
left: Castasterone
name: Castasterone → 26-Hydroxycastasterone
conversion direction: LEFT_TO_RIGHT

Reaction211392
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108364/Stoichiometry/1.0
participant stoichiometry: Compound/PW C108363/Stoichiometry/1.0
right: Castasterone
spontaneous: false
left: 6a-Hydroxycastasterone
name: 6a-Hydroxycastasterone → Castasterone
conversion direction: LEFT_TO_RIGHT

Reaction211391
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108364/Stoichiometry/1.0
participant stoichiometry: Compound/PW C015039/Stoichiometry/1.0
right: 6a-Hydroxycastasterone
spontaneous: false
left: 6-Deoxocastasterone
name: 6-Deoxocastasterone → 6a-Hydroxycastasterone
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis200242
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction210596
control type: ACTIVATION

ChemicalStructure/PW C007677
type: ChemicalStructure
structure format: SMILES

Compound/PW C001721 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campesterol
stoichiometric coefficient: 1.0

ChemicalStructure/PW C007673
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C007674
type: ChemicalStructure
structure format: SMILES

FMN
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: FMN

Compound/PW C000143/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADP
stoichiometric coefficient: 1.0

ReactionCatalysis177062
type: Catalysis
controller: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
controlled: Reaction187346
control type: ACTIVATION

ReactionCatalysis177063
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction187347
control type: ACTIVATION

Compound/PW C015469/Stoichiometry/1.0
type: Stoichiometry
physical entity: 2-Deoxycastasterone
stoichiometric coefficient: 1.0

ReactionCatalysis177064
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction187348
control type: ACTIVATION

Reference/HMDB HMDB0012112
type: UnificationXref
id: HMDB0012112
db: HMDB

Reference/HMDB HMDB0012113
type: UnificationXref
id: HMDB0012113
db: HMDB

Reference/SMPDB SMP0122321
type: UnificationXref
id: SMP0122321
db: SMPDB

Reference/HMDB HMDB0012116
type: UnificationXref
id: HMDB0012116
db: HMDB

Compound/PW C007677/Stoichiometry/1.0
type: Stoichiometry
physical entity: (5α)-campestan-3-one
stoichiometric coefficient: 1.0

3-Epi-6-deoxocathesterone
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: 3-Epi-6-deoxocathesterone

Reaction210596
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108362/Stoichiometry/1.0
participant stoichiometry: Compound/PW C022318/Stoichiometry/1.0
right: 3-Dehydroteasterone
spontaneous: false
left: 3-Dehydro-6-deoxo-teasterone
name: 3-Dehydro-6-deoxo-teasterone → 3-Dehydroteasterone
conversion direction: LEFT_TO_RIGHT

Water
type: SmallMolecule
entity reference: Water

ReactionCatalysis199665
type: Catalysis
controller: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
controlled: Reaction210018
control type: ACTIVATION

ChemicalStructure/PW C105111
type: ChemicalStructure
structure format: SMILES

Reference/PathWhiz PW123631
type: UnificationXref
id: PW123631
db: PathWhiz

ChemicalStructure/PW C105112
type: ChemicalStructure
structure format: SMILES

Reference/SMPDB SMP0121236
type: UnificationXref
id: SMP0121236
db: SMPDB

ChemicalStructure/PW C105116
type: ChemicalStructure
structure format: SMILES

Compound/PW C105119/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxocathasterone
stoichiometric coefficient: 1.0

FMN
type: SmallMolecule
entity reference: FMN

ChemicalStructure/PW C105113
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C105114
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C105119
type: ChemicalStructure
structure format: SMILES

Reference/PubChem-compound 22833530
type: UnificationXref
id: 22833530
db: PubChem-compound

Compound/PW C015136 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Brassinolide
stoichiometric coefficient: 1.0

Compound/PW C007673 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22α)-hydroxy-5α-campestan-3-one
stoichiometric coefficient: 1.0

ChemicalStructure/PW C105117
type: ChemicalStructure
structure format: SMILES

Reference/KEGG Compound C11049
type: UnificationXref
id: C11049
db: KEGG Compound

ChemicalStructure/PW C105118
type: ChemicalStructure
structure format: SMILES

ReactionCatalysis201039
type: Catalysis
controller: Cytochrome P450 734A1
controlled: Reaction211395
control type: ACTIVATION

Compound/PW C105120/Stoichiometry/1.0
type: Stoichiometry
physical entity: Cathasterone
stoichiometric coefficient: 1.0

Reaction210009
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108362/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105122/Stoichiometry/1.0
right: 3-Dehydro-6-deoxo-teasterone
spontaneous: false
left: 6-Deoxoteasterone
name: 6-Deoxoteasterone → 3-Dehydro-6-deoxo-teasterone
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis201035
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction211391
control type: ACTIVATION

ReactionCatalysis201036
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction211392
control type: ACTIVATION

ReactionCatalysis201037
type: Catalysis
controller: Cytochrome P450 734A1
controlled: 1.14.-.-
control type: ACTIVATION

ReactionCatalysis201038
type: Catalysis
controller: Cytochrome P450 85A2
controlled: Reaction211394
control type: ACTIVATION

ReactionCatalysis201033
type: Catalysis
controller: Chaperonin CPN60-like 1, mitochondrial
controlled: Reaction211389
control type: ACTIVATION

ReactionCatalysis201034
type: Catalysis
controller: Chaperonin CPN60-like 1, mitochondrial
controlled: Reaction211390
control type: ACTIVATION

Compound/PW C105118/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Oxocampestanol
stoichiometric coefficient: 1.0

22alpha-Hydroxycampesterol
type: SmallMolecule
cellular location: Cell Membrane
entity reference: 22alpha-Hydroxycampesterol

ReactionCatalysis199656
type: Catalysis
controller: Cytochrome P450 90D2
controlled: Reaction210009
control type: ACTIVATION

ChemicalStructure/PW C105122
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C105123
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C105120
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C105121
type: ChemicalStructure
structure format: SMILES

Reference/CAS 87833-54-3
type: UnificationXref
id: 87833-54-3
db: CAS

3-Dehydroteasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 14353978
xref: Reference/HMDB HMDB0041527
xref: Reference/CAS 124853-28-7
xref: Reference/ChemSpider 21234468
chemical formula: C28H46O4
molecular weight: 446.3396
structure: ChemicalStructure/PW C022318

Reference/KEGG Compound C15793
type: UnificationXref
id: C15793
db: KEGG Compound

Reaction210018
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001420/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007673/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000891/Stoichiometry/1.0
participant stoichiometry: Compound/PW C104784/Stoichiometry/1.0
participant stoichiometry: Compound/PW C108362/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
right: FMNH2
right: 3-Dehydro-6-deoxo-teasterone
right: Oxygen
spontaneous: false
left: Water
left: (22α)-hydroxy-5α-campestan-3-one
left: Hydrogen Ion
left: FMN
name: (22α)-hydroxy-5α-campestan-3-one + FMN + Hydrogen Ion + Water → 3-Dehydro-6-deoxo-teasterone + FMNH2 + Oxygen
conversion direction: LEFT_TO_RIGHT

Compound/PW C108363 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: Castasterone
stoichiometric coefficient: 1.0

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

26-Hydroxybrassinolide
type: SmallMolecule
cellular location: Membrane
entity reference: 26-Hydroxybrassinolide

Compound/PW C001420 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Water
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: Water

Reference/KEGG Compound C00061
type: UnificationXref
id: C00061
db: KEGG Compound

Cytochrome P450 90A1
type: Protein
cellular location: Membrane
entity reference: Cytochrome P450 90A1

FMNH2
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 16048
xref: Reference/BioCyc FMNH2
xref: Reference/KEGG Compound C01847
xref: Reference/PubChem-compound 445395
xref: Reference/CAS 5666-16-0
xref: Reference/ChemSpider 393046
xref: Reference/HMDB HMDB0001142
chemical formula: C17H23N4O9P
molecular weight: 458.12027
structure: ChemicalStructure/PW C000891

Reference/PubChem-compound 44229199
type: UnificationXref
id: 44229199
db: PubChem-compound

Compound/PW C007677 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: (5α)-campestan-3-one
stoichiometric coefficient: 1.0

Compound/PW C000143 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADP
stoichiometric coefficient: 1.0

Compound/PW C105112 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 3-Epi-6-deoxocathesterone
stoichiometric coefficient: 1.0

Campestanol
type: SmallMoleculeReference
xref: Reference/ChemSpider 17215967
xref: Reference/HMDB HMDB0000534
xref: Reference/CAS 474-60-2
xref: Reference/PubChem-compound 22833530
chemical formula: C28H50O
molecular weight: 402.38617
structure: ChemicalStructure/PW C000411

(5α)-campestan-3-one
type: SmallMolecule
cellular location: Membrane
entity reference: (5α)-campestan-3-one

Reference/ChemSpider 21234468
type: UnificationXref
id: 21234468
db: ChemSpider

Reference/HMDB HMDB0001377
type: UnificationXref
id: HMDB0001377
db: HMDB

Reference/PathWhiz PW122531
type: UnificationXref
id: PW122531
db: PathWhiz

Compound/PW C104784/Stoichiometry/1.0
type: Stoichiometry
physical entity: FMN
stoichiometric coefficient: 1.0

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Reference/ChemSpider 17215967
type: UnificationXref
id: 17215967
db: ChemSpider

ChemicalStructure/PW C015469
type: ChemicalStructure
structure format: SMILES

7-Oxateasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 46173984
chemical formula: C28H48O5
molecular weight: 464.3502
structure: ChemicalStructure/PW C105123

Teasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 13475125
chemical formula: C28H48O4
molecular weight: 448.35526
structure: ChemicalStructure/PW C105121

6-Deoxoteasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 11144580
chemical formula: C28H50O3
molecular weight: 434.376
structure: ChemicalStructure/PW C105122

Reference/CAS 124853-28-7
type: UnificationXref
id: 124853-28-7
db: CAS

6-Deoxocastasterone
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: 6-Deoxocastasterone

Cathasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 15341086
chemical formula: C28H48O3
molecular weight: 432.36035
structure: ChemicalStructure/PW C105120

Compound/PW C040034 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00080
type: UnificationXref
id: C00080
db: KEGG Compound

ChemicalStructure/PW C001065
type: ChemicalStructure
structure format: SMILES
structure data: O=O

Compound/PW C000411 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campestanol
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0001142
type: UnificationXref
id: HMDB0001142
db: HMDB

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

Compound/PW C015469 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: 2-Deoxycastasterone
stoichiometric coefficient: 1.0

6-Oxocampestanol
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 20747
xref: Reference/PubChem-compound 16061347
chemical formula: C28H48O2
molecular weight: 416.36542
structure: ChemicalStructure/PW C105118

6-Deoxocathasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 16061344
chemical formula: C28H50O2
molecular weight: 418.38107
structure: ChemicalStructure/PW C105119

(22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
type: SmallMoleculeReference
xref: Reference/PubChem-compound 52931381
chemical formula: C28H46O3
molecular weight: 430.3447
structure: ChemicalStructure/PW C105116

6a-hydroxycampestanol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 16061348
chemical formula: C28H50O2
molecular weight: 418.38107
structure: ChemicalStructure/PW C105117

Campest-4-en-3beta-ol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 11988278
chemical formula: C28H48O
molecular weight: 400.3705
structure: ChemicalStructure/PW C105114

3-Epi-6-deoxocathesterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 45480560
chemical formula: C28H50O2
molecular weight: 418.38107
structure: ChemicalStructure/PW C105112

6-Deoxotyphasterol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 16061346
chemical formula: C28H50O3
molecular weight: 434.376
structure: ChemicalStructure/PW C105113

Compound/PW C007674 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22α)-hydroxy-campest-4-en-3-one
stoichiometric coefficient: 1.0

(22R,23R)-22,23-Dihydroxycampesterol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 11048310
chemical formula: C28H48O3
molecular weight: 432.36035
structure: ChemicalStructure/PW C105111

Reference/PubChem-compound 11144580
type: UnificationXref
id: 11144580
db: PubChem-compound

Compound/PW C108365 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 26-Hydroxycastasterone
stoichiometric coefficient: 1.0

22alpha-Hydroxycampesterol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 23724587
chemical formula: C28H48O2
molecular weight: 416.36542
structure: ChemicalStructure/PW C105109

Reference/PubChem-compound 133534
type: UnificationXref
id: 133534
db: PubChem-compound

Castasterone
type: SmallMolecule
cellular location: Membrane
entity reference: Castasterone

Reference/PubChem-compound 16061346
type: UnificationXref
id: 16061346
db: PubChem-compound

Compound/PW C105112/Stoichiometry/1.0
type: Stoichiometry
physical entity: 3-Epi-6-deoxocathesterone
stoichiometric coefficient: 1.0

Reference/PubChem-compound 16061347
type: UnificationXref
id: 16061347
db: PubChem-compound

Reference/PubChem-compound 16061344
type: UnificationXref
id: 16061344
db: PubChem-compound

FMNH2
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: FMNH2

Reference/PubChem-compound 16061345
type: UnificationXref
id: 16061345
db: PubChem-compound

Reference/PubChem-compound 16061348
type: UnificationXref
id: 16061348
db: PubChem-compound

Reference/PubChem-compound 22833512
type: UnificationXref
id: 22833512
db: PubChem-compound

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

(22α)-hydroxy-campest-4-en-3-one
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: (22α)-hydroxy-campest-4-en-3-one

Reference/ChemSpider 393046
type: UnificationXref
id: 393046
db: ChemSpider

Reference/HMDB HMDB0034081
type: UnificationXref
id: HMDB0034081
db: HMDB

Campestanol
type: SmallMolecule
cellular location: Membrane
entity reference: Campestanol

Reference/UniProt Q42569
type: UnificationXref
id: Q42569
db: UniProt

Compound/PW C007746/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campest-4-en-3-one
stoichiometric coefficient: 1.0

Compound/PW C001721/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campesterol
stoichiometric coefficient: 1.0

Reference/PubChem-compound 977
type: UnificationXref
id: 977
db: PubChem-compound

Reference/HMDB HMDB0059597
type: UnificationXref
id: HMDB0059597
db: HMDB

Reference/PubChem-compound 443055
type: UnificationXref
id: 443055
db: PubChem-compound

Reference/PubChem-compound 67550006
type: UnificationXref
id: 67550006
db: PubChem-compound

Reference/PubChem-compound 46173985
type: UnificationXref
id: 46173985
db: PubChem-compound

Compound/PW C108363 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Castasterone
stoichiometric coefficient: 1.0

Reference/PubChem-compound 46173984
type: UnificationXref
id: 46173984
db: PubChem-compound

Cytochrome P450 90D2
type: Protein
entity reference: Cytochrome P450 90D2

Compound/PW C108361/Stoichiometry/1.0
type: Stoichiometry
physical entity: 7-Oxatyphasterol
stoichiometric coefficient: 1.0

6-Deoxocathasterone
type: SmallMolecule
entity reference: 6-Deoxocathasterone

Reference/ChemSpider 17215925
type: UnificationXref
id: 17215925
db: ChemSpider

Compound/PW C105119 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxocathasterone
stoichiometric coefficient: 1.0

Reference/TAXONOMY 3702
type: UnificationXref
id: 3702
db: TAXONOMY

Reference/ChEBI CHEBI 58210
type: UnificationXref
id: CHEBI:58210
db: ChEBI

Reference/HMDB HMDB0034423
type: UnificationXref
id: HMDB0034423
db: HMDB

Reference/HMDB HMDB0000534
type: UnificationXref
id: HMDB0000534
db: HMDB

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

ChemicalStructure/PW C104784
type: ChemicalStructure
structure format: SMILES

6-Deoxotyphasterol
type: SmallMolecule
entity reference: 6-Deoxotyphasterol

Campest-4-en-3beta-ol
type: SmallMolecule
entity reference: Campest-4-en-3beta-ol

(22R,23R)-22,23-Dihydroxycampesterol
type: SmallMolecule
entity reference: (22R,23R)-22,23-Dihydroxycampesterol

3-Epi-6-deoxocathesterone
type: SmallMolecule
entity reference: 3-Epi-6-deoxocathesterone

6a-hydroxycampestanol
type: SmallMolecule
entity reference: 6a-hydroxycampestanol

6-Oxocampestanol
type: SmallMolecule
entity reference: 6-Oxocampestanol

Reference/PubChem-compound 5283637
type: UnificationXref
id: 5283637
db: PubChem-compound

(22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
type: SmallMolecule
entity reference: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one

1.14.-.-
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105121/Stoichiometry/1.0
participant stoichiometry: Compound/PW C022318/Stoichiometry/1.0
right: 3-Dehydroteasterone
spontaneous: false
EC number: 1.14.-.-
left: Teasterone
name: Teasterone → 3-Dehydroteasterone
conversion direction: LEFT_TO_RIGHT

Campest-4-en-3-one
type: SmallMolecule
cellular location: Membrane
entity reference: Campest-4-en-3-one

Compound/PW C108362/Stoichiometry/1.0
type: Stoichiometry
physical entity: 3-Dehydro-6-deoxo-teasterone
stoichiometric coefficient: 1.0

Compound/PW C105111/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22R,23R)-22,23-Dihydroxycampesterol
stoichiometric coefficient: 1.0

Compound/PW C108363/Stoichiometry/1.0
type: Stoichiometry
physical entity: Castasterone
stoichiometric coefficient: 1.0

Reference/CAS 53-59-8
type: UnificationXref
id: 53-59-8
db: CAS

Compound/PW C108364/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6a-Hydroxycastasterone
stoichiometric coefficient: 1.0

Reaction187306
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007746/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000146/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007677/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000143/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
right: (5α)-campestan-3-one
right: NADP
spontaneous: false
left: Campest-4-en-3-one
left: Hydrogen Ion
left: NADPH
name: Campest-4-en-3-one + Hydrogen Ion + NADPH → (5α)-campestan-3-one + NADP
conversion direction: LEFT_TO_RIGHT

Brassinolide
type: SmallMolecule
cellular location: Membrane
entity reference: Brassinolide

Reference/PubChem-compound 1038
type: UnificationXref
id: 1038
db: PubChem-compound

Reference/UniProt Q940V4
type: UnificationXref
id: Q940V4
db: UniProt

FMNH2
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: FMNH2

1.14.-.-
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105109 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007674 Membrane/Stoichiometry/1.0
right: (22α)-hydroxy-campest-4-en-3-one
spontaneous: false
EC number: 1.14.-.-
left: 22alpha-Hydroxycampesterol
name: 22alpha-Hydroxycampesterol → (22α)-hydroxy-campest-4-en-3-one
conversion direction: LEFT_TO_RIGHT

1.3.1.22
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000143 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007674 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000146 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007673 Membrane/Stoichiometry/1.0
right: NADP
right: (22α)-hydroxy-5α-campestan-3-one
spontaneous: false
EC number: 1.3.1.22
left: Hydrogen Ion
left: (22α)-hydroxy-campest-4-en-3-one
left: NADPH
name: (22α)-hydroxy-campest-4-en-3-one + Hydrogen Ion + NADPH → (22α)-hydroxy-5α-campestan-3-one + NADP
conversion direction: LEFT_TO_RIGHT

Reaction187302
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007673/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105112/Stoichiometry/1.0
right: 3-Epi-6-deoxocathesterone
spontaneous: false
left: (22α)-hydroxy-5α-campestan-3-one
name: (22α)-hydroxy-5α-campestan-3-one ↔ 3-Epi-6-deoxocathesterone
conversion direction: REVERSIBLE

1.14.14.147
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105112 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C104784 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105113 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000891 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
right: Water
right: 6-Deoxotyphasterol
right: FMN
right: Hydrogen Ion
spontaneous: false
EC number: 1.14.14.147
left: Oxygen
left: FMNH2
left: 3-Epi-6-deoxocathesterone
name: 3-Epi-6-deoxocathesterone + FMNH2 + Oxygen → 6-Deoxotyphasterol + FMN + Hydrogen Ion + Water
conversion direction: LEFT_TO_RIGHT

Reaction187304
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001721/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105114/Stoichiometry/1.0
right: Campest-4-en-3beta-ol
spontaneous: false
left: Campesterol
name: Campesterol → Campest-4-en-3beta-ol
conversion direction: LEFT_TO_RIGHT

Reaction187305
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007746/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105114/Stoichiometry/1.0
right: Campest-4-en-3-one
spontaneous: false
left: Campest-4-en-3beta-ol
name: Campest-4-en-3beta-ol → Campest-4-en-3-one
conversion direction: LEFT_TO_RIGHT

Compound/PW C105113/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxotyphasterol
stoichiometric coefficient: 1.0

Reference/PubChem-compound 445395
type: UnificationXref
id: 445395
db: PubChem-compound

Reference/PubChem-compound 25201374
type: UnificationXref
id: 25201374
db: PubChem-compound

NADPH
type: SmallMolecule
entity reference: NADPH

Reference/GENE ONTOLOGY GO 0070258
type: UnificationXref
id: GO:0070258
db: GENE ONTOLOGY

Reference/PubChem-compound 11048310
type: UnificationXref
id: 11048310
db: PubChem-compound

Cathasterone
type: SmallMolecule
entity reference: Cathasterone

Compound/PW C104784 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: FMN
stoichiometric coefficient: 1.0

Teasterone
type: SmallMolecule
entity reference: Teasterone

NADP
type: SmallMolecule
entity reference: NADP

Compound/PW C000411/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campestanol
stoichiometric coefficient: 1.0

Hydrogen Ion
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: Hydrogen Ion

NADPH
type: SmallMoleculeReference
xref: Reference/CAS 53-57-6
xref: Reference/HMDB HMDB0000221
xref: Reference/PubChem-compound 22833512
xref: Reference/BioCyc NADPH
xref: Reference/ChemSpider 17215925
xref: Reference/KEGG Compound C00005
xref: Reference/ChEBI CHEBI 16474
chemical formula: C21H30N7O17P3
molecular weight: 745.0911
structure: ChemicalStructure/PW C000146

6-Deoxoteasterone
type: SmallMolecule
entity reference: 6-Deoxoteasterone

NADP
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00006
xref: Reference/CAS 53-59-8
xref: Reference/BioCyc NAD(P)
xref: Reference/HMDB HMDB0000217
xref: Reference/ChEBI CHEBI 18009
xref: Reference/PubChem-compound 5886
xref: Reference/ChemSpider 5675
chemical formula: C21H29N7O17P3
molecular weight: 744.08325
structure: ChemicalStructure/PW C000143

7-Oxateasterone
type: SmallMolecule
entity reference: 7-Oxateasterone

2-Deoxycastasterone
type: SmallMolecule
entity reference: 2-Deoxycastasterone

Compound/PW C108366 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 26-Hydroxybrassinolide
stoichiometric coefficient: 1.0

Reference/PubChem-compound 14353978
type: UnificationXref
id: 14353978
db: PubChem-compound

Reference/BioCyc NADPH
type: UnificationXref
id: NADPH
db: BioCyc

Compound/PW C015039 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxocastasterone
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0002869
type: UnificationXref
id: HMDB0002869
db: HMDB

Reference/BioCyc CPD-6641
type: UnificationXref
id: CPD-6641
db: BioCyc

Castasterone
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Castasterone

26-Hydroxycastasterone
type: SmallMolecule
cellular location: Membrane
entity reference: 26-Hydroxycastasterone

Cytochrome P450 90B1
type: ProteinReference
xref: Reference/UniProt O64989
organism: Arabidopsis thaliana

3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
type: ProteinReference
xref: Reference/UniProt Q94IA6
organism: Arabidopsis thaliana

FMN
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: FMN

SubPathwayInteraction112412
type: Interaction
name: SubPathway112412Reaction
name: SubPathwayReaction
participant: Campesterol

Reference/HMDB HMDB0000217
type: UnificationXref
id: HMDB0000217
db: HMDB

Oxygen
type: SmallMolecule
entity reference: Oxygen

Reference/GENE ONTOLOGY GO 0005886
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

Oxygen
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: Oxygen

FMN
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00061
xref: Reference/ChEBI CHEBI 58210
xref: Reference/PubChem-compound 44229199
chemical formula: C17H18N4O9P
molecular weight: 453.0828
structure: ChemicalStructure/PW C104784

Inner Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0070258

ChemicalStructure/PW C000143
type: ChemicalStructure
structure format: SMILES

6-Deoxotyphasterol
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: 6-Deoxotyphasterol

Compound/PW C007674 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22α)-hydroxy-campest-4-en-3-one
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0000221
type: UnificationXref
id: HMDB0000221
db: HMDB

Reaction210609
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105113/Stoichiometry/1.0
participant stoichiometry: Compound/PW C108362/Stoichiometry/1.0
right: 6-Deoxotyphasterol
spontaneous: false
left: 3-Dehydro-6-deoxo-teasterone
name: 3-Dehydro-6-deoxo-teasterone ↔ 6-Deoxotyphasterol
conversion direction: REVERSIBLE

ChemicalStructure/PW C000146
type: ChemicalStructure
structure format: SMILES

Reference/GENE ONTOLOGY GO 0005759
type: UnificationXref
id: GO:0005759
db: GENE ONTOLOGY

Cytochrome P450 85A2
type: ProteinReference
xref: Reference/UniProt Q940V4

Cytochrome P450 90A1
type: ProteinReference
xref: Reference/UniProt Q42569
organism: Arabidopsis thaliana

Teasterone
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: Teasterone

Steroid 5-alpha-reductase DET2
type: ProteinReference
xref: Reference/UniProt Q38944
organism: Arabidopsis thaliana

Compound/PW C105113 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxotyphasterol
stoichiometric coefficient: 1.0

Reference/CAS 72962-43-7
type: UnificationXref
id: 72962-43-7
db: CAS

Reference/CAS 53-57-6
type: UnificationXref
id: 53-57-6
db: CAS

Compound/PW C015136 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Brassinolide
stoichiometric coefficient: 1.0

Reference/PubChem-compound 13870433
type: UnificationXref
id: 13870433
db: PubChem-compound

1.14.-.-
type: BiochemicalReaction
participant stoichiometry: Compound/PW C108361/Stoichiometry/1.0
participant stoichiometry: Compound/PW C015469/Stoichiometry/1.0
right: 7-Oxatyphasterol
spontaneous: false
EC number: 1.14.-.-
left: 2-Deoxycastasterone
name: 2-Deoxycastasterone → 7-Oxatyphasterol
conversion direction: LEFT_TO_RIGHT

Reaction210612
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105113/Stoichiometry/1.0
participant stoichiometry: Compound/PW C015469/Stoichiometry/1.0
right: 2-Deoxycastasterone
spontaneous: false
left: 6-Deoxotyphasterol
name: 6-Deoxotyphasterol → 2-Deoxycastasterone
conversion direction: LEFT_TO_RIGHT

Reaction210611
type: BiochemicalReaction
participant stoichiometry: Compound/PW C022318/Stoichiometry/1.0
participant stoichiometry: Compound/PW C015469/Stoichiometry/1.0
right: 2-Deoxycastasterone
spontaneous: false
left: 3-Dehydroteasterone
name: 3-Dehydroteasterone → 2-Deoxycastasterone
conversion direction: LEFT_TO_RIGHT

(22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one

Reaction210610
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007746 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007674 Membrane/Stoichiometry/1.0
right: (22α)-hydroxy-campest-4-en-3-one
spontaneous: false
left: Campest-4-en-3-one
name: Campest-4-en-3-one → (22α)-hydroxy-campest-4-en-3-one
conversion direction: LEFT_TO_RIGHT

Reaction187342
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105119 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000411 Membrane/Stoichiometry/1.0
right: 6-Deoxocathasterone
spontaneous: false
left: Campestanol
name: Campestanol → 6-Deoxocathasterone
conversion direction: LEFT_TO_RIGHT

Chaperonin CPN60-like 1, mitochondrial
type: ProteinReference
xref: Reference/UniProt Q8L7B5
organism: Arabidopsis thaliana

Reaction187343
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105118/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105120/Stoichiometry/1.0
right: Cathasterone
spontaneous: false
left: 6-Oxocampestanol
name: 6-Oxocampestanol → Cathasterone
conversion direction: LEFT_TO_RIGHT

Cytochrome P450 734A1
type: ProteinReference
xref: Reference/UniProt O48786
organism: Arabidopsis thaliana

1.14.14.147
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105121 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C104784 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105120 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000891 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Endoplasmic Reticulum/Stoichiometry/1.0
right: Water
right: Hydrogen Ion
right: Teasterone
right: FMN
spontaneous: false
EC number: 1.14.14.147
left: FMNH2
left: Cathasterone
left: Oxygen
name: Cathasterone + FMNH2 + Oxygen → FMN + Hydrogen Ion + Teasterone + Water
conversion direction: LEFT_TO_RIGHT

Hydrogen Ion
type: SmallMolecule
cellular location: Membrane
entity reference: Hydrogen Ion

Reaction187346
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105122 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C104784 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000891 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105119 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Endoplasmic Reticulum/Stoichiometry/1.0
right: Water
right: Hydrogen Ion
right: 6-Deoxoteasterone
right: FMN
spontaneous: false
left: FMNH2
left: Oxygen
left: 6-Deoxocathasterone
name: 6-Deoxocathasterone + FMNH2 + Oxygen → 6-Deoxoteasterone + FMN + Hydrogen Ion + Water
conversion direction: LEFT_TO_RIGHT

Reaction187347
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105121/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105122/Stoichiometry/1.0
right: Teasterone
spontaneous: false
left: 6-Deoxoteasterone
name: 6-Deoxoteasterone → Teasterone
conversion direction: LEFT_TO_RIGHT

Reaction187348
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105121/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105123/Stoichiometry/1.0
right: 7-Oxateasterone
spontaneous: false
left: Teasterone
name: Teasterone → 7-Oxateasterone
conversion direction: LEFT_TO_RIGHT

Cytochrome P450 90D2
type: ProteinReference
xref: Reference/UniProt Q94IW5

6-Deoxocathasterone
type: SmallMolecule
cellular location: Membrane
entity reference: 6-Deoxocathasterone

ReactionCatalysis199640
type: Catalysis
controller: Cytochrome P450 90D2
controlled: 1.14.-.-
control type: ACTIVATION

Reaction187340
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105118/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105117/Stoichiometry/1.0
right: 6-Oxocampestanol
spontaneous: false
left: 6a-hydroxycampestanol
name: 6a-hydroxycampestanol → 6-Oxocampestanol
conversion direction: LEFT_TO_RIGHT

Reaction187341
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007673/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105119/Stoichiometry/1.0
right: 6-Deoxocathasterone
spontaneous: false
left: (22α)-hydroxy-5α-campestan-3-one
name: (22α)-hydroxy-5α-campestan-3-one → 6-Deoxocathasterone
conversion direction: LEFT_TO_RIGHT

Compound/PW C105113 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxotyphasterol
stoichiometric coefficient: 1.0

Cytochrome P450 85A2
type: Protein
cellular location: Inner Membrane
entity reference: Cytochrome P450 85A2

Compound/PW C007746 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campest-4-en-3-one
stoichiometric coefficient: 1.0

Reaction187339
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000411/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105117/Stoichiometry/1.0
right: 6a-hydroxycampestanol
spontaneous: false
left: Campestanol
name: Campestanol → 6a-hydroxycampestanol
conversion direction: LEFT_TO_RIGHT

Campesterol
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Campesterol
name: SubPathwayOutput

Reaction187334
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007677 Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007673 Membrane/Stoichiometry/1.0
right: (22α)-hydroxy-5α-campestan-3-one
spontaneous: false
left: (5α)-campestan-3-one
name: (5α)-campestan-3-one → (22α)-hydroxy-5α-campestan-3-one
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 27722
type: UnificationXref
id: CHEBI:27722
db: ChEBI

Reaction187336
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105111/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105116/Stoichiometry/1.0
right: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
spontaneous: false
left: (22R,23R)-22,23-Dihydroxycampesterol
name: (22R,23R)-22,23-Dihydroxycampesterol → (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
conversion direction: LEFT_TO_RIGHT

Reaction187337
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007674 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105116 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C104784 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000891 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
right: Water
right: FMN
right: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
right: Hydrogen Ion
spontaneous: false
left: Oxygen
left: (22α)-hydroxy-campest-4-en-3-one
left: FMNH2
name: (22α)-hydroxy-campest-4-en-3-one + FMNH2 + Oxygen → (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one + FMN + Hydrogen Ion + Water
conversion direction: LEFT_TO_RIGHT

Reaction187338
type: BiochemicalReaction
participant stoichiometry: Compound/PW C007677/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000411/Stoichiometry/1.0
right: Campestanol
spontaneous: false
left: (5α)-campestan-3-one
name: (5α)-campestan-3-one → Campestanol
conversion direction: LEFT_TO_RIGHT

Compound/PW C105114/Stoichiometry/1.0
type: Stoichiometry
physical entity: Campest-4-en-3beta-ol
stoichiometric coefficient: 1.0

ChemicalStructure/PW C105109
type: ChemicalStructure
structure format: SMILES

Reference/UniProt Q94IA6
type: UnificationXref
id: Q94IA6
db: UniProt

Reference/PubChem-compound 15341086
type: UnificationXref
id: 15341086
db: PubChem-compound

6-Deoxocastasterone
type: SmallMolecule
entity reference: 6-Deoxocastasterone

Reference/ChemSpider 4446730
type: UnificationXref
id: 4446730
db: ChemSpider

Endoplasmic Reticulum
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005783

Endoplasmic Reticulum Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005789

Compound/PW C105122/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxoteasterone
stoichiometric coefficient: 1.0

Campest-4-en-3-one
type: SmallMoleculeReference
xref: Reference/PubChem-compound 25200612
xref: Reference/ChEBI CHEBI 18534
xref: Reference/BioCyc CPD-3945
xref: Reference/HMDB HMDB0012196
chemical formula: C28H46O
molecular weight: 398.35486
structure: ChemicalStructure/PW C007746

Compound/PW C000891/Stoichiometry/1.0
type: Stoichiometry
physical entity: FMNH2
stoichiometric coefficient: 1.0

Mitochondrial Matrix
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005759

Compound/PW C000146 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: NADPH
stoichiometric coefficient: 1.0

Compound/PW C105109 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: 22alpha-Hydroxycampesterol
stoichiometric coefficient: 1.0

Brassinolide
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 27722
xref: Reference/PubChem-compound 443055
xref: Reference/HMDB HMDB0034081
xref: Reference/ChemSpider 391354
xref: Reference/CAS 72962-43-7
xref: Reference/KEGG Compound C11049
chemical formula: C28H48O6
molecular weight: 480.3451
structure: ChemicalStructure/PW C015136

Compound/PW C105119 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6-Deoxocathasterone
stoichiometric coefficient: 1.0

Reference/ChemSpider 5675
type: UnificationXref
id: 5675
db: ChemSpider

Reference/PubChem-compound 11988278
type: UnificationXref
id: 11988278
db: PubChem-compound

Reference/PubChem-compound 52931381
type: UnificationXref
id: 52931381
db: PubChem-compound

2-Deoxycastasterone
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: 2-Deoxycastasterone

Compound/PW C105121/Stoichiometry/1.0
type: Stoichiometry
physical entity: Teasterone
stoichiometric coefficient: 1.0

Water
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: Water

Brassinolide
type: SmallMolecule
cellular location: Inner Membrane
entity reference: Brassinolide

Reference/ChEBI CHEBI 19809
type: UnificationXref
id: CHEBI:19809
db: ChEBI

Reference/UniProt O48786
type: UnificationXref
id: O48786
db: UniProt

Compound/PW C105117/Stoichiometry/1.0
type: Stoichiometry
physical entity: 6a-hydroxycampestanol
stoichiometric coefficient: 1.0

2-Deoxycastasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 13475117
xref: Reference/KEGG Compound C15793
xref: Reference/CAS 87734-68-7
xref: Reference/ChemSpider 21142762
xref: Reference/HMDB HMDB0034423
chemical formula: C28H48O4
molecular weight: 448.35526
structure: ChemicalStructure/PW C015469

Compound/PW C105116 Endoplasmic Reticulum Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
stoichiometric coefficient: 1.0

Arabidopsis thaliana
type: BioSource
xref: Reference/TAXONOMY 3702

Castasterone
type: SmallMolecule
entity reference: Castasterone

Campest-4-en-3-one
type: SmallMolecule
entity reference: Campest-4-en-3-one

6a-Hydroxycastasterone
type: SmallMolecule
entity reference: 6a-Hydroxycastasterone

Oxygen
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001377
xref: Reference/ChemSpider 952
xref: Reference/KEGG Compound C00007
xref: Reference/ChEBI CHEBI 15379
xref: Reference/PubChem-compound 977
xref: Reference/BioCyc CPD-6641
xref: Reference/CAS 7782-44-7
chemical formula: O2
molecular weight: 31.98983
structure: ChemicalStructure/PW C001065

Reference/GENE ONTOLOGY GO 0005789
type: UnificationXref
id: GO:0005789
db: GENE ONTOLOGY

7-Oxatyphasterol
type: SmallMolecule
entity reference: 7-Oxatyphasterol

3-Dehydro-6-deoxo-teasterone
type: SmallMolecule
entity reference: 3-Dehydro-6-deoxo-teasterone

Reference/GENE ONTOLOGY GO 0005783
type: UnificationXref
id: GO:0005783
db: GENE ONTOLOGY

Compound/PW C105123/Stoichiometry/1.0
type: Stoichiometry
physical entity: 7-Oxateasterone
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0033984
type: UnificationXref
id: HMDB0033984
db: HMDB

NADP
type: SmallMolecule
cellular location: Membrane
entity reference: NADP

Reference/CAS 7782-44-7
type: UnificationXref
id: 7782-44-7
db: CAS

Reference/PubChem-compound 25202189
type: UnificationXref
id: 25202189
db: PubChem-compound

Reference/GENE ONTOLOGY GO 0016020
type: UnificationXref
id: GO:0016020
db: GENE ONTOLOGY

Compound/PW C007673/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22α)-hydroxy-5α-campestan-3-one
stoichiometric coefficient: 1.0

22alpha-Hydroxycampesterol
type: SmallMolecule
cellular location: Membrane
entity reference: 22alpha-Hydroxycampesterol

Reference/UniProt Q38944
type: UnificationXref
id: Q38944
db: UniProt

Reference/ChemSpider 391354
type: UnificationXref
id: 391354
db: ChemSpider

Reference/ChEBI CHEBI 20712
type: UnificationXref
id: CHEBI:20712
db: ChEBI

Compound/PW C001065 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: Oxygen
stoichiometric coefficient: 1.0

Reference/KEGG Compound C01847
type: UnificationXref
id: C01847
db: KEGG Compound

ChemicalStructure/PW C022318
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C000891
type: ChemicalStructure
structure format: SMILES

7-Oxatyphasterol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 46173985
chemical formula: C28H48O5
molecular weight: 464.3502
structure: ChemicalStructure/PW C108361

3-Dehydro-6-deoxo-teasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 16061345
chemical formula: C28H48O3
molecular weight: 432.36035
structure: ChemicalStructure/PW C108362

Castasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 133534
chemical formula: C28H48O5
molecular weight: 464.3502
structure: ChemicalStructure/PW C108363

ChemicalStructure/PW C000411
type: ChemicalStructure
structure format: SMILES

6a-Hydroxycastasterone
type: SmallMoleculeReference
xref: Reference/PubChem-compound 15542699
chemical formula: C28H50O5
molecular weight: 466.3658
structure: ChemicalStructure/PW C108364

26-Hydroxycastasterone
type: SmallMoleculeReference
xref: Reference/ChemSpider 58827458
xref: Reference/PubChem-compound 101408509
chemical formula: C28H48O6
molecular weight: 480.3451
structure: ChemicalStructure/PW C108365

26-Hydroxybrassinolide
type: SmallMoleculeReference
xref: Reference/PubChem-compound 67550006
chemical formula: C28H48O7
molecular weight: 496.34
structure: ChemicalStructure/PW C108366

Cell Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005886

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Reference/ChEBI CHEBI 15379
type: UnificationXref
id: CHEBI:15379
db: ChEBI

Reference/ChEBI CHEBI 15378
type: UnificationXref
id: CHEBI:15378
db: ChEBI

ChemicalStructure/PW C040034
type: ChemicalStructure
structure format: SMILES
structure data: [H+]

Compound/PW C105116/Stoichiometry/1.0
type: Stoichiometry
physical entity: (22R, 23R)-22,23-dihydroxy-campest-4-en-3-one
stoichiometric coefficient: 1.0

Reference/ChemSpider 58827458
type: UnificationXref
id: 58827458
db: ChemSpider

(22R,23R)-22,23-Dihydroxycampesterol
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: (22R,23R)-22,23-Dihydroxycampesterol

Reference/CAS 4651-51-8
type: UnificationXref
id: 4651-51-8
db: CAS

Reference/PubChem-compound 5886
type: UnificationXref
id: 5886
db: PubChem-compound

(5α)-campestan-3-one
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0012116
xref: Reference/BioCyc CPD-3946
xref: Reference/PubChem-compound 25201374
chemical formula: C28H48O
molecular weight: 400.3705
structure: ChemicalStructure/PW C007677

(5α)-campestan-3-one
type: SmallMolecule
entity reference: (5α)-campestan-3-one

Compound/PW C001420/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

(22α)-hydroxy-campest-4-en-3-one
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0012113
xref: Reference/PubChem-compound 25201842
chemical formula: C28H46O2
molecular weight: 414.3498
structure: ChemicalStructure/PW C007674

Compound/PW C105121 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: Teasterone
stoichiometric coefficient: 1.0

(22α)-hydroxy-5α-campestan-3-one
type: SmallMolecule
entity reference: (22α)-hydroxy-5α-campestan-3-one

Cathasterone
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: Cathasterone

(22α)-hydroxy-5α-campestan-3-one
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0012112
xref: Reference/PubChem-compound 25202189
chemical formula: C28H48O2
molecular weight: 416.36542
structure: ChemicalStructure/PW C007673

Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0016020

Chaperonin CPN60-like 1, mitochondrial
type: Protein
cellular location: Mitochondrial Matrix
entity reference: Chaperonin CPN60-like 1, mitochondrial

6-Deoxotyphasterol
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: 6-Deoxotyphasterol

Compound/PW C001420 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

(22α)-hydroxy-5α-campestan-3-one
type: SmallMolecule
cellular location: Membrane
entity reference: (22α)-hydroxy-5α-campestan-3-one

Reference/BioCyc FMNH2
type: UnificationXref
id: FMNH2
db: BioCyc

Compound/PW C105109 Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 22alpha-Hydroxycampesterol
stoichiometric coefficient: 1.0

Compound/PW C105120 Endoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: Cathasterone
stoichiometric coefficient: 1.0

3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
type: Protein
cellular location: Endoplasmic Reticulum
entity reference: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1

Compound/PW C108363 Inner Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Castasterone
stoichiometric coefficient: 1.0

6-Deoxocastasterone
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 20712
xref: Reference/KEGG Compound C15802
xref: Reference/PubChem-compound 13870433
xref: Reference/ChemSpider 21864867
xref: Reference/CAS 87833-54-3
xref: Reference/HMDB HMDB0033984
chemical formula: C28H50O4
molecular weight: 450.3709
structure: ChemicalStructure/PW C015039

ChemicalStructure/PW C001721
type: ChemicalStructure
structure format: SMILES

Compound/PW C105109 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 22alpha-Hydroxycampesterol
stoichiometric coefficient: 1.0

6-Deoxocathasterone
type: SmallMolecule
cellular location: Endoplasmic Reticulum
entity reference: 6-Deoxocathasterone

1.14.-.-
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001721 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105109 Cell Membrane/Stoichiometry/1.0
right: 22alpha-Hydroxycampesterol
spontaneous: false
EC number: 1.14.-.-
left: Campesterol
name: Campesterol → 22alpha-Hydroxycampesterol
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 45480560
type: UnificationXref
id: 45480560
db: PubChem-compound

1.14.14.147
type: BiochemicalReaction
participant stoichiometry: Compound/PW C105109 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C105111 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C104784 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001065 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000891 Endoplasmic Reticulum/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Endoplasmic Reticulum/Stoichiometry/1.0
right: Water
right: (22R,23R)-22,23-Dihydroxycampesterol
right: Hydrogen Ion
right: FMN
spontaneous: false
EC number: 1.14.14.147
left: FMNH2
left: 22alpha-Hydroxycampesterol
left: Oxygen
name: 22alpha-Hydroxycampesterol + FMNH2 + Oxygen → (22R,23R)-22,23-Dihydroxycampesterol + FMN + Hydrogen Ion + Water
conversion direction: LEFT_TO_RIGHT

Steroid 5-alpha-reductase DET2
type: Protein
cellular location: Membrane
entity reference: Steroid 5-alpha-reductase DET2

Reference/PubChem-compound 101408509
type: UnificationXref
id: 101408509
db: PubChem-compound