Carbonic acid
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0003538
xref: Reference/BioCyc CARBON-DIOXIDE
xref: Reference/ChEBI CHEBI 28976
xref: Reference/PubChem-compound 22639876
xref: Reference/KEGG Compound C01353
xref: Reference/CAS 463-79-6
xref: Reference/ChemSpider 747
chemical formula: CH2O3
molecular weight: 62.000393
structure: ChemicalStructure/PW C001916

Adenylate cyclase type 10
type: Protein
cellular location: Cytoplasm
entity reference: Adenylate cyclase type 10

Adenylate cyclase type 10
type: ProteinReference
xref: Reference/UniProt Q96PN6
organism: BiologicalState658

cAMP-dependent protein kinase catalytic subunit beta
type: ProteinReference
xref: Reference/UniProt P22694
organism: BiologicalState658

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Stomach
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0001307

direct_interaction
type: InteractionVocabulary

Reference/UniProt P00915
type: UnificationXref
id: P00915
db: UniProt

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Reference/UniProt P20648
type: UnificationXref
id: P20648
db: UniProt

Apical cell membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0016324

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

ChemicalStructure/PW C000457
type: ChemicalStructure
structure format: SMILES
structure data: [K+]

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

Solute carrier family 12 member 6
type: ProteinReference
xref: Reference/UniProt Q9UHW9

Liver carboxylesterase 1
type: ProteinReference
xref: Reference/UniProt P23141
organism: BiologicalState26

Misoprostol Acid
type: SmallMoleculeReference
xref: Reference/ChemSpider 4941043
chemical formula: C21H36O5
molecular weight: 368.2563

Water
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Water

ChemicalStructure/PW C000463
type: ChemicalStructure
structure format: SMILES
structure data: OC(O)=O

Reference/ChemSpider 559142
type: UnificationXref
id: 559142
db: ChemSpider

Compound/PW C009374 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Misoprostol
stoichiometric coefficient: 1.0

cAMP
type: SmallMoleculeReference
xref: Reference/PubChem-compound 6076
xref: Reference/HMDB HMDB0000058
xref: Reference/ChEBI CHEBI 17489
xref: Reference/CAS 60-92-4
xref: Reference/BioCyc CAMP
xref: Reference/ChemSpider 5851
xref: Reference/KEGG Compound C00575
chemical formula: C10H12N5O6P
molecular weight: 329.05252
structure: ChemicalStructure/PW C000041

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase catalytic subunit beta

TransportCatalysis4856
type: Catalysis
controller: Chloride anion exchanger
controlled: Transport13823
control type: ACTIVATION

Compound/PW C113636 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Misoprostol Acid
stoichiometric coefficient: 1.0

Reference/CELL TYPE ONTOLOGY CL 0000182
type: UnificationXref
id: CL:0000182
db: CELL TYPE ONTOLOGY

Compound/PW C000041 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP
stoichiometric coefficient: 1.0

Chloride anion exchanger
type: ProteinReference
xref: Reference/UniProt P40879

Reference/BRENDA TISSUE ONTOLOGY BTO 0000759
type: UnificationXref
id: BTO:0000759
db: BRENDA TISSUE ONTOLOGY

Reference/BioCyc HCO3
type: UnificationXref
id: HCO3
db: BioCyc

Compound/PW C000457 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

Reference/PubChem-compound 1038
type: UnificationXref
id: 1038
db: PubChem-compound

Reference/KEGG Compound C00698
type: UnificationXref
id: C00698
db: KEGG Compound

Reference/KEGG Compound C00575
type: UnificationXref
id: C00575
db: KEGG Compound

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Carbon dioxide
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Carbon dioxide

Reference/ChemSpider 274
type: UnificationXref
id: 274
db: ChemSpider

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase type I-alpha regulatory subunit

Compound/PW C113636 Basal Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Misoprostol Acid
stoichiometric coefficient: 1.0

Compound/PW C000457 Apical cell membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Chloride ion
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 17996
xref: Reference/BioCyc CPD-4521
xref: Reference/PubChem-compound 312
xref: Reference/KEGG Compound C00698
xref: Reference/HMDB HMDB0000492
xref: Reference/ChemSpider 306
xref: Reference/CAS 16887-00-6
chemical formula: Cl
molecular weight: 34.968853
structure: ChemicalStructure/PW C000374

H(+)/K(+) ATPase subunit
type: Complex
component stoichiometry: Protein/1354 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/1340 Cytoplasm/Stoichiometry/1.0
cellular location: Cytoplasm
component: Potassium-transporting ATPase alpha chain 1
component: Potassium-transporting ATPase subunit beta

Compound/PW C000463 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen carbonate
stoichiometric coefficient: 1.0

Reference/PubChem-compound 813
type: UnificationXref
id: 813
db: PubChem-compound

Reference/UniProt Q96PN6
type: UnificationXref
id: Q96PN6
db: UniProt

Interaction4961
type: MolecularInteraction
interaction type: direct_interaction
name: Misoprostol Acid activates Prostaglandin E2 receptor EP3 subtype
participant: Prostaglandin E2 receptor EP3 subtype
participant: Misoprostol Acid

Reference/ChemSpider 5851
type: UnificationXref
id: 5851
db: ChemSpider

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase type II-alpha regulatory subunit

Reaction217855
type: BiochemicalReaction
participant stoichiometry: Compound/PW C040034 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000463 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001916 Cytoplasm/Stoichiometry/1.0
right: Hydrogen Ion
right: Hydrogen carbonate
spontaneous: false
left: Carbonic acid
name: Carbonic acid → Hydrogen Ion + Hydrogen carbonate
conversion direction: LEFT_TO_RIGHT

Reference/ChemSpider 1010
type: UnificationXref
id: 1010
db: ChemSpider

Potassium-transporting ATPase subunit beta
type: ProteinReference
xref: Reference/UniProt P51164

Reference/ChemSpider 4941043
type: UnificationXref
id: 4941043
db: ChemSpider

Reference/BioCyc PPI
type: UnificationXref
id: PPI
db: BioCyc

Interaction3758
type: MolecularInteraction
interaction type: direct_interaction
name: Protein kinase A activates H(+)/K(+) ATPase subunit
participant: Protein kinase A
participant: H(+)/K(+) ATPase subunit

ChemicalStructure/PW C009374
type: ChemicalStructure
structure format: SMILES

Reference/KEGG Compound C00238
type: UnificationXref
id: C00238
db: KEGG Compound

Protein/1990 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-alpha regulatory subunit
stoichiometric coefficient: 1.0

Potassium-transporting ATPase subunit beta
type: Protein
cellular location: Cytoplasm
entity reference: Potassium-transporting ATPase subunit beta

Compound/PW C040034 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Compound/PW C000374 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Chloride ion
stoichiometric coefficient: 1.0

Potassium-transporting ATPase alpha chain 1
type: ProteinReference
xref: Reference/UniProt P20648

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

ChemicalStructure/PW C000374
type: ChemicalStructure
structure format: SMILES
structure data: [Cl-]

Chloride ion
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Chloride ion

Protein/3358 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-alpha regulatory subunit
stoichiometric coefficient: 1.0

Reference/UniProt P13861
type: UnificationXref
id: P13861
db: UniProt

Reference/CAS 124-38-9
type: UnificationXref
id: 124-38-9
db: CAS

Parietal Cell
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000162

Misoprostol
type: SmallMolecule
cellular location: Cytosol
entity reference: Misoprostol

TransportCatalysis2050
type: Catalysis
controller: Solute carrier family 12 member 6
controlled: Transport10880
control type: ACTIVATION

Potassium-transporting ATPase alpha chain 1
type: Protein
cellular location: Cytoplasm
entity reference: Potassium-transporting ATPase alpha chain 1

Reference/PubChem-compound 312
type: UnificationXref
id: 312
db: PubChem-compound

Protein/1262 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit alpha
stoichiometric coefficient: 1.0

Protein/3357 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-beta regulatory subunit
stoichiometric coefficient: 1.0

Reference/CAS 24203-36-9
type: UnificationXref
id: 24203-36-9
db: CAS

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Reference/UniProt P22694
type: UnificationXref
id: P22694
db: UniProt

Carbonic acid
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Carbonic acid

Reference/ChEBI CHEBI 18361
type: UnificationXref
id: CHEBI:18361
db: ChEBI

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Liver
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000759

Reference/HMDB HMDB0000586
type: UnificationXref
id: HMDB0000586
db: HMDB

cAMP-dependent protein kinase type I-beta regulatory subunit
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase type I-beta regulatory subunit

Protein kinase A
type: Complex
component stoichiometry: Protein/1990 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/1262 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/1315 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/3357 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/3358 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/3359 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/1134 Cytoplasm/Stoichiometry/1.0
cellular location: Cytoplasm
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase type I-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit alpha
component: cAMP-dependent protein kinase catalytic subunit gamma
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit beta

Reference/ChEBI CHEBI 17489
type: UnificationXref
id: CHEBI:17489
db: ChEBI

Compound/PW C000170 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Pyrophosphate
stoichiometric coefficient: 1.0

Reference/BioCyc CAMP
type: UnificationXref
id: CAMP
db: BioCyc

Protein/3359 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-beta regulatory subunit
stoichiometric coefficient: 1.0

Adenosine triphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Adenosine triphosphate

Reference/KEGG Compound C00011
type: UnificationXref
id: C00011
db: KEGG Compound

ChemicalStructure/PW C001316
type: ChemicalStructure
structure format: SMILES
structure data: O=C=O

cAMP-dependent protein kinase catalytic subunit gamma
type: ProteinReference
xref: Reference/UniProt P22612
organism: BiologicalState658

Gi signaling cascade
type: Pathway
name: InhibitorySubPathway

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Reference/KEGG Compound C00013
type: UnificationXref
id: C00013
db: KEGG Compound

Compound/PW C009374/Stoichiometry/1.0
type: Stoichiometry
physical entity: Misoprostol
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0000595
type: UnificationXref
id: HMDB0000595
db: HMDB

Pyrophosphate
type: SmallMoleculeReference
xref: Reference/PubChem-compound 644102
xref: Reference/BioCyc PPI
xref: Reference/ChemSpider 559142
xref: Reference/ChEBI CHEBI 18361
xref: Reference/HMDB HMDB0000250
xref: Reference/CAS 14000-31-8
xref: Reference/KEGG Compound C00013
chemical formula: H4O7P2
molecular weight: 177.94322
structure: ChemicalStructure/PW C000170

Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

Reference/CAS 463-79-6
type: UnificationXref
id: 463-79-6
db: CAS

Hepatocyte
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000182

Hydrogen Ion
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00080
xref: Reference/ChEBI CHEBI 15378
xref: Reference/HMDB HMDB0059597
xref: Reference/PubChem-compound 1038
xref: Reference/ChemSpider 1010
chemical formula: H
molecular weight: 1.007825
structure: ChemicalStructure/PW C040034

Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Reference/KEGG Compound C01353
type: UnificationXref
id: C01353
db: KEGG Compound

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

cAMP-dependent protein kinase catalytic subunit alpha
type: ProteinReference
xref: Reference/UniProt P17612
organism: BiologicalState658

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

Reference/CAS 60-92-4
type: UnificationXref
id: 60-92-4
db: CAS

Prostaglandin E2 receptor EP3 subtype
type: Protein
cellular location: Basal Cell Membrane
entity reference: Prostaglandin E2 receptor EP3 subtype
name: SubPathwayInhibitor

Reference/ChEBI CHEBI 29103
type: UnificationXref
id: CHEBI:29103
db: ChEBI

Reaction110775
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000041 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000170 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
right: Pyrophosphate
right: cAMP
spontaneous: false
left: Adenosine triphosphate
name: Adenosine triphosphate → Pyrophosphate + cAMP
conversion direction: LEFT_TO_RIGHT

cAMP
type: SmallMolecule
cellular location: Cytoplasm
entity reference: cAMP

BiologicalState26
type: BioSource
cell type: Hepatocyte
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Liver, Hepatocyte
tissue: Liver

Reference/PubChem-compound 769
type: UnificationXref
id: 769
db: PubChem-compound

Reference/HMDB HMDB0015064
type: UnificationXref
id: HMDB0015064
db: HMDB

Reference/PubChem-compound 5282381
type: UnificationXref
id: 5282381
db: PubChem-compound

Adenylate cyclase type 10
type: Protein
cellular location: Basal Cell Membrane
entity reference: Adenylate cyclase type 10
name: SubPathwayInhibition

Compound/PW C001316 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Carbon dioxide
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 122391
type: UnificationXref
id: CHEBI:122391
db: ChEBI

Reference/HMDB HMDB0000492
type: UnificationXref
id: HMDB0000492
db: HMDB

Reference/HMDB HMDB0000250
type: UnificationXref
id: HMDB0000250
db: HMDB

Potassium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Potassium

ChemicalStructure/PW C000170
type: ChemicalStructure
structure format: SMILES
structure data: OP(O)(=O)OP(O)(O)=O

Reference/CAS 14000-31-8
type: UnificationXref
id: 14000-31-8
db: CAS

Reference/ChEBI CHEBI 17996
type: UnificationXref
id: CHEBI:17996
db: ChEBI

Liver carboxylesterase 1
type: Protein
cellular location: Cytosol
entity reference: Liver carboxylesterase 1

Reference/BioCyc CARBON-DIOXIDE
type: UnificationXref
id: CARBON-DIOXIDE
db: BioCyc

Transport13823
type: Transport
participant stoichiometry: Compound/PW C000374 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000463 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000374 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000463 Extracellular Space/Stoichiometry/1.0
right: Hydrogen carbonate
right: Chloride ion
spontaneous: false
left: Hydrogen carbonate
left: Chloride ion
name: Chloride ion (→), Hydrogen carbonate (←) Transport: Homo sapiens, Stomach, Extracellular Space to Homo sapiens, Stomach, Parietal Cell, Cytoplasm
conversion direction: LEFT_TO_RIGHT

Protein/1134 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit gamma
stoichiometric coefficient: 1.0

ReactionCatalysis208902
type: Catalysis
controller: Liver carboxylesterase 1
controlled: Reaction219504
control type: ACTIVATION

Reference/BioCyc K+
type: UnificationXref
id: K%2b
db: BioCyc

Compound/PW C001916 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Carbonic acid
stoichiometric coefficient: 1.0

Reference/CAS 16887-00-6
type: UnificationXref
id: 16887-00-6
db: CAS

Reference/KEGG Compound C00288
type: UnificationXref
id: C00288
db: KEGG Compound

Reference/HMDB HMDB0003538
type: UnificationXref
id: HMDB0003538
db: HMDB

Reference/PubChem-compound 22639876
type: UnificationXref
id: 22639876
db: PubChem-compound

ReactionCatalysis101096
type: Catalysis
controller: Adenylate cyclase type 10
controlled: Reaction110775
control type: ACTIVATION

Misoprostol
type: SmallMoleculeReference
xref: Reference/ChemSpider 4445541
xref: Reference/HMDB HMDB0015064
xref: Reference/ChEBI CHEBI 122391
xref: Reference/CAS 59122-46-2
xref: Reference/PubChem-compound 5282381
chemical formula: C22H38O5
molecular weight: 382.2719
structure: ChemicalStructure/PW C009374

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

Hydrogen Ion
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Hydrogen Ion

Misoprostol
type: SmallMolecule
entity reference: Misoprostol

Basal Cell Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0009925

Hydrogen carbonate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Hydrogen carbonate

ChemicalStructure/PW C001916
type: ChemicalStructure
structure format: SMILES
structure data: OC(O)=O

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

cAMP-dependent protein kinase type II-beta regulatory subunit
type: ProteinReference
xref: Reference/UniProt P31323
organism: BiologicalState658

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: ProteinReference
xref: Reference/UniProt P13861
organism: BiologicalState658

Homo sapiens
type: BioSource
xref: Reference/TAXONOMY 9606

cAMP-dependent protein kinase catalytic subunit gamma
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase catalytic subunit gamma

cAMP-dependent protein kinase type I-beta regulatory subunit
type: ProteinReference
xref: Reference/UniProt P31321
organism: BiologicalState658

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Reference/CAS 59122-46-2
type: UnificationXref
id: 59122-46-2
db: CAS

Reaction219504
type: BiochemicalReaction
participant stoichiometry: Compound/PW C113636 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C009374 Cytosol/Stoichiometry/1.0
right: Misoprostol Acid
spontaneous: false
left: Misoprostol
name: Misoprostol → Misoprostol Acid
conversion direction: LEFT_TO_RIGHT

Interaction2835
type: MolecularInteraction
interaction type: direct_interaction
name: cAMP activates Protein kinase A
participant: Protein kinase A
participant: cAMP

Compound/PW C000374 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Chloride ion
stoichiometric coefficient: 1.0

Hydrogen Ion
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Hydrogen Ion

Misoprostol Acid
type: SmallMolecule
cellular location: Cytosol
entity reference: Misoprostol Acid

Carbonic anhydrase 1
type: Protein
cellular location: Cytoplasm
entity reference: Carbonic anhydrase 1

Protein/1315 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit beta
stoichiometric coefficient: 1.0

Reference/PathWhiz PW126203
type: UnificationXref
id: PW126203
db: PathWhiz

Reference/ChemSpider 791
type: UnificationXref
id: 791
db: ChemSpider

Reference/UniProt P40879
type: UnificationXref
id: P40879
db: UniProt

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

TransportCatalysis2047
type: Catalysis
controller: H(+)/K(+) ATPase subunit
controlled: Transport10877
control type: ACTIVATION

Reference/ChEBI CHEBI 16526
type: UnificationXref
id: CHEBI:16526
db: ChEBI

Reference/ChemSpider 306
type: UnificationXref
id: 306
db: ChemSpider

Pyrophosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Pyrophosphate

ChemicalStructure/PW C000041
type: ChemicalStructure
structure format: SMILES

Reference/ChEBI CHEBI 28976
type: UnificationXref
id: CHEBI:28976
db: ChEBI

Reference/UniProt P43115
type: UnificationXref
id: P43115
db: UniProt

Hydrogen Ion
type: SmallMolecule
cellular location: Apical cell membrane
entity reference: Hydrogen Ion

Reference/SMPDB SMP0124718
type: UnificationXref
id: SMP0124718
db: SMPDB

Reference/TAXONOMY 9606
type: UnificationXref
id: 9606
db: TAXONOMY

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Potassium
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Potassium

Reference/ChEBI CHEBI 17544
type: UnificationXref
id: CHEBI:17544
db: ChEBI

Compound/PW C113636/Stoichiometry/1.0
type: Stoichiometry
physical entity: Misoprostol Acid
stoichiometric coefficient: 1.0

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: ProteinReference
xref: Reference/UniProt P10644
organism: BiologicalState658

Carbon dioxide
type: SmallMoleculeReference
xref: Reference/PubChem-compound 280
xref: Reference/ChEBI CHEBI 16526
xref: Reference/ChemSpider 274
xref: Reference/HMDB HMDB0001967
xref: Reference/CAS 124-38-9
xref: Reference/KEGG Compound C00011
chemical formula: CO2
molecular weight: 43.98983
structure: ChemicalStructure/PW C001316

Potassium
type: SmallMolecule
cellular location: Apical cell membrane
entity reference: Potassium

Reference/CELL TYPE ONTOLOGY CL 0000162
type: UnificationXref
id: CL:0000162
db: CELL TYPE ONTOLOGY

cAMP-dependent protein kinase type II-beta regulatory subunit
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase type II-beta regulatory subunit

Reference/KEGG Compound C00080
type: UnificationXref
id: C00080
db: KEGG Compound

Transport10880
type: Transport
participant stoichiometry: Compound/PW C000374 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000374 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Cytoplasm/Stoichiometry/1.0
right: Chloride ion
right: Potassium
spontaneous: true
left: Potassium
left: Chloride ion
name: Potassium (←), Chloride ion (←) Transport: Homo sapiens, Stomach, Extracellular Space to Homo sapiens, Stomach, Parietal Cell, Cytoplasm
conversion direction: REVERSIBLE

Reference/HMDB HMDB0000058
type: UnificationXref
id: HMDB0000058
db: HMDB

Transport15770
type: Transport
participant stoichiometry: Compound/PW C113636/Stoichiometry/1.0
participant stoichiometry: Compound/PW C113636 Basal Cell Membrane/Stoichiometry/1.0
right: Misoprostol Acid
spontaneous: false
left: Misoprostol Acid
name: Misoprostol Acid (→) Transport: Homo sapiens, Blood Vessel to Homo sapiens, Stomach, Parietal Cell, Basal Cell Membrane
conversion direction: LEFT_TO_RIGHT

cAMP-dependent protein kinase catalytic subunit alpha
type: Protein
cellular location: Cytoplasm
entity reference: cAMP-dependent protein kinase catalytic subunit alpha

Misoprostol Action Pathway (New)
type: Pathway
pathway order: PathwayEnzymeReaction/2501559
pathway order: PathwayEnzymeReaction/2501558
pathway order: PathwaySubPathway/116839
pathway order: PathwayTransport/2501557
pathway order: PathwayTransport/2501553
pathway order: PathwayTransport/2501555
pathway order: PathwayTransport/2533944
pathway order: PathwayTransport/2533967
pathway order: PathwayInteraction/2835
pathway order: PathwayInteraction/3758
pathway order: PathwayEnzymeReaction/2533965
pathway order: PathwayEnzymeReaction/2501561
pathway order: PathwayInteraction/4961
pathway order: PathwayTransport/2533961
pathway order: PathwayTransport/2533973
pathway component: TransportCatalysis4856
pathway component: TransportCatalysis2047
pathway component: Reaction217751
pathway component: Transport13823
pathway component: ReactionCatalysis208902
pathway component: Interaction4961
pathway component: Transport10877
pathway component: Reaction217855
pathway component: Reaction219504
pathway component: Transport15766
pathway component: Transport15767
pathway component: Interaction2835
pathway component: Transport15764
pathway component: Transport10880
pathway component: ReactionCatalysis207276
pathway component: SubPathwayControl116839
pathway component: ReactionCatalysis101096
pathway component: Interaction3758
pathway component: Transport15770
pathway component: Reaction110775
pathway component: TransportCatalysis2050
xref: Reference/PathWhiz PW126203
xref: Reference/SMPDB SMP0124718
organism: Homo sapiens

Solute carrier family 12 member 6
type: Protein
entity reference: Solute carrier family 12 member 6

Hydrogen carbonate
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Hydrogen carbonate

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Reference/UniProt P31321
type: UnificationXref
id: P31321
db: UniProt

Reference/UniProt P10644
type: UnificationXref
id: P10644
db: UniProt

Reference/ChEBI CHEBI 15378
type: UnificationXref
id: CHEBI:15378
db: ChEBI

ChemicalStructure/PW C040034
type: ChemicalStructure
structure format: SMILES
structure data: [H+]

H(+)/K(+) ATPase subunit
type: Complex
component stoichiometry: Protein/1340/Stoichiometry/1.0
component stoichiometry: Protein/1354/Stoichiometry/1.0
component: Potassium-transporting ATPase subunit beta
component: Potassium-transporting ATPase alpha chain 1

Reference/UniProt Q9UHW9
type: UnificationXref
id: Q9UHW9
db: UniProt

Reference/UniProt P31323
type: UnificationXref
id: P31323
db: UniProt

Reaction217751
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001316 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001916 Cytoplasm/Stoichiometry/1.0
right: Carbonic acid
spontaneous: false
left: Carbon dioxide
left: Water
name: Carbon dioxide + Water → Carbonic acid
conversion direction: LEFT_TO_RIGHT

Chloride anion exchanger
type: Protein
entity reference: Chloride anion exchanger

Carbonic anhydrase 1
type: ProteinReference
xref: Reference/UniProt P00915
organism: BiologicalState658

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Transport10877
type: Transport
participant stoichiometry: Compound/PW C040034 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Apical cell membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Apical cell membrane/Stoichiometry/1.0
right: Hydrogen Ion
right: Potassium
spontaneous: false
left: Hydrogen Ion
left: Potassium
name: Hydrogen Ion (→), Potassium (←) Transport: Homo sapiens, Stomach, Parietal Cell, Apical cell membrane to Homo sapiens, Stomach, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Reference/UniProt P23141
type: UnificationXref
id: P23141
db: UniProt

Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Transport15766
type: Transport
participant stoichiometry: Compound/PW C009374 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C009374/Stoichiometry/1.0
right: Misoprostol
spontaneous: false
left: Misoprostol
name: Misoprostol (→) Transport: Homo sapiens, Blood Vessel to Homo sapiens, Liver, Hepatocyte, Cytosol
conversion direction: LEFT_TO_RIGHT

Transport15767
type: Transport
participant stoichiometry: Compound/PW C113636 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C113636/Stoichiometry/1.0
right: Misoprostol Acid
spontaneous: false
left: Misoprostol Acid
name: Misoprostol Acid (→) Transport: Homo sapiens, Liver, Hepatocyte, Cytosol to Homo sapiens, Blood Vessel
conversion direction: LEFT_TO_RIGHT

Transport15764
type: Transport
participant stoichiometry: Compound/PW C009374/Stoichiometry/1.0
right: Misoprostol
spontaneous: false
left: Misoprostol
name: Misoprostol (→) Transport: Homo sapiens, Intestine to Homo sapiens, Blood Vessel
conversion direction: LEFT_TO_RIGHT

Compound/PW C040034 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/UniProt P22612
type: UnificationXref
id: P22612
db: UniProt

Misoprostol Acid
type: SmallMolecule
entity reference: Misoprostol Acid

Protein/1354 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium-transporting ATPase alpha chain 1
stoichiometric coefficient: 1.0

Reference/PubChem-compound 280
type: UnificationXref
id: 280
db: PubChem-compound

Protein/1354/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium-transporting ATPase alpha chain 1
stoichiometric coefficient: 1.0

Reference/BRENDA TISSUE ONTOLOGY BTO 0001307
type: UnificationXref
id: BTO:0001307
db: BRENDA TISSUE ONTOLOGY

Compound/PW C040034 Apical cell membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Reference/BioCyc CPD-4521
type: UnificationXref
id: CPD-4521
db: BioCyc

Reference/PubChem-compound 644102
type: UnificationXref
id: 644102
db: PubChem-compound

Reference/ChemSpider 4445541
type: UnificationXref
id: 4445541
db: ChemSpider

Reference/GENE ONTOLOGY GO 0016324
type: UnificationXref
id: GO:0016324
db: GENE ONTOLOGY

Prostaglandin E2 receptor EP3 subtype
type: ProteinReference
xref: Reference/UniProt P43115
organism: BiologicalState658

Chloride ion
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Chloride ion

Potassium-transporting ATPase subunit beta
type: Protein
entity reference: Potassium-transporting ATPase subunit beta

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Protein/1340 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium-transporting ATPase subunit beta
stoichiometric coefficient: 1.0

SubPathwayControl116839
type: Interaction
name: SubPathway116839Control
name: InhibitorySubPathwayControl
participant: Prostaglandin E2 receptor EP3 subtype
participant: Gi signaling cascade
participant: Adenylate cyclase type 10

Reference/UniProt P51164
type: UnificationXref
id: P51164
db: UniProt

BiologicalState658
type: BioSource
cell type: Parietal Cell
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Stomach, Parietal Cell
tissue: Stomach

Reference/PubChem-compound 6076
type: UnificationXref
id: 6076
db: PubChem-compound

Reference/HMDB HMDB0059597
type: UnificationXref
id: HMDB0059597
db: HMDB

Potassium
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00238
xref: Reference/HMDB HMDB0000586
xref: Reference/PubChem-compound 813
xref: Reference/ChemSpider 791
xref: Reference/CAS 24203-36-9
xref: Reference/BioCyc K+
xref: Reference/ChEBI CHEBI 29103
chemical formula: K
molecular weight: 38.963707
structure: ChemicalStructure/PW C000457

Reference/GENE ONTOLOGY GO 0009925
type: UnificationXref
id: GO:0009925
db: GENE ONTOLOGY

Reference/HMDB HMDB0001967
type: UnificationXref
id: HMDB0001967
db: HMDB

Reference/ChemSpider 749
type: UnificationXref
id: 749
db: ChemSpider

Reference/CAS 71-52-3
type: UnificationXref
id: 71-52-3
db: CAS

Compound/PW C000463 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen carbonate
stoichiometric coefficient: 1.0

Reference/ChemSpider 747
type: UnificationXref
id: 747
db: ChemSpider

Protein/1340/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium-transporting ATPase subunit beta
stoichiometric coefficient: 1.0

ReactionCatalysis207276
type: Catalysis
controller: Carbonic anhydrase 1
controlled: Reaction217751
control type: ACTIVATION

Reference/UniProt P17612
type: UnificationXref
id: P17612
db: UniProt

Potassium-transporting ATPase alpha chain 1
type: Protein
entity reference: Potassium-transporting ATPase alpha chain 1

Hydrogen carbonate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000595
xref: Reference/ChEBI CHEBI 17544
xref: Reference/PubChem-compound 769
xref: Reference/BioCyc HCO3
xref: Reference/ChemSpider 749
xref: Reference/CAS 71-52-3
xref: Reference/KEGG Compound C00288
chemical formula: CH2O3
molecular weight: 62.000393
structure: ChemicalStructure/PW C000463

Misoprostol Acid
type: SmallMolecule
cellular location: Basal Cell Membrane
entity reference: Misoprostol Acid