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Sodium/chloride cotransporter Aquaporin-2 Aquaporin-2 Transient receptor potential cation channel subfamily V member 5 ATP-binding cassette sub-family C member 8 ATP-sensitive inward rectifier potassium channel 8 ATP-sensitive inward rectifier potassium channel 11 ATP-binding cassette sub-family C member 9 Sodium/calcium exchanger 3 Chloride channel protein ClC-Ka Sodium/potassium- transporting ATPase subunit alpha-1 Sodium/potassium- transporting ATPase subunit alpha-2 Sodium/potassium- transporting ATPase subunit alpha-3 Sodium/potassium- transporting ATPase subunit alpha-4 Sodium/potassium- transporting ATPase subunit beta-1 Sodium/potassium- transporting ATPase subunit beta-2 Sodium/potassium- transporting ATPase subunit beta-3 ATPase, (Na+)/K+ transporting, beta 4 polypeptide Sodium/potassium- transporting ATPase subunit gamma Multidrug resistance- associated protein 4 Solute carrier family 22 member 6 Benzthiazide H2O H2O H2O Ca+ Ca+ K+ K+ Na+ Na+ Cl- Cl- Ca+ Ca+ Na+ Na+ Cl- Cl- Na+ Na+ K+ K+ Benzthiazide Benzthiazide Benzthiazide Distal convoluted tubule Lumen Benzthiazide inhibits the sodium/chloride transporter Cytosolic Na+ and Cl- decrease Benzthiazide is transported from the blood into the epithelial cells of the distal convoluted tubule of the nephron. Epithelial cell The concentration of Na+ and Cl- builds up in the lumen/urine. The concentration gradient between the epithelial cells and the lumen is decreased. Due to the high concentrations of solute in the lumen, less water reabsorption from the filtrate. More water is excreted in the urine. Blood Vessel The low cytosolic Na+ reduces the activity of the Na+/K+ ATPase. As a result, more K+ accumulates in the cytosol and is transported to the lumen for excretion. Kidney
SLC12A3 AQP2 AQP2 TRPV5 ABCC8 KCNJ8 KCNJ11 Unknown SLC8A3 CLCNKA ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 Unknown FXYD2 ABCC4 SLC22A6 Benzthiazide Water Water Water Calcium Calcium Potassium Potassium Sodium Sodium Chloride ion Chloride ion Calcium Calcium Sodium Sodium Chloride ion Chloride ion Sodium Sodium Potassium Potassium Benzthiazide Benzthiazide Benzthiazide
SLC12A3 AQP2 AQP2 TRPV5 ABCC8 KCNJ8 KCNJ11 SLC8A3 CLCNKA ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 FXYD2 ABCC4 SLC22A6 Benzthi H2O H2O H2O Ca+ Ca+ K+ K+ Na+ Na+ Cl- Cl- Ca+ Ca+ Na+ Na+ Cl- Cl- Na+ Na+ K+ K+ Benzthi Benzthi Benzthi Distal convoluted tubule Lumen Benzthiazide inhibits the sodium/chloride transporter Cytosolic Na+ and Cl- decrease Benzthiazide is transported from the blood into the epithelial cells of the distal convoluted tubule of the nephron. Epithelial cell The concentration of Na+ and Cl- builds up in the lumen/urine. The concentration gradient between the epithelial cells and the lumen is decreased. Due to the high concentrations of solute in the lumen, less water reabsorption from the filtrate. More water is excreted in the urine. Blood Vessel The low cytosolic Na+ reduces the activity of the Na+/K+ ATPase. As a result, more K+ accumulates in the cytosol and is transported to the lumen for excretion. Kidney
SLC12A3 AQP2 AQP2 TRPV5 ABCC8 KCNJ8 KCNJ11 SLC8A3 CLCNKA ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 FXYD2 ABCC4 SLC22A6 Benzthi H2O H2O H2O Ca2+ Ca2+ K+ K+ Na+ Na+ Cl Cl Ca2+ Ca2+ Na+ Na+ Cl Cl Na+ Na+ K+ K+ Benzthi Benzthi Benzthi