Reference/UniProt Q6UA68
type: UnificationXref
id: Q6UA68
db: UniProt

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
type: Protein
cellular location: Cytoplasm
entity reference: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

cAMP-dependent protein kinase catalytic subunit beta
type: ProteinReference
xref: Reference/UniProt P22694
organism: BiologicalState1512

Reference/UniProt P0DP31
type: UnificationXref
id: P0DP31
db: UniProt

cAMP
type: SmallMolecule
cellular location: Cytosol
entity reference: cAMP

direct_interaction
type: InteractionVocabulary

Transport16266
type: Transport
participant stoichiometry: Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Rattus norvegicus, Extracellular Space to Rattus norvegicus, Lung
conversion direction: LEFT_TO_RIGHT

ProteinComplex/11046/Stoichiometry/1.0
type: Stoichiometry
physical entity: Protein kinase A
stoichiometric coefficient: 1.0

Reference/UniProt Q8K1M2
type: UnificationXref
id: Q8K1M2
db: UniProt

Protein/19764/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-beta regulatory subunit
stoichiometric coefficient: 1.0

G protein complex
type: Complex
cellular location: Cytoplasm
component: Gγ
component: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
component: Guanine nucleotide-binding protein G(i) subunit alpha-1

Inositol 1,4,5-trisphosphate receptor type 1
type: ProteinReference
xref: Reference/UniProt Q62869
organism: BiologicalState1512

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit beta

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

Calcium
type: SmallMolecule
cellular location: Cytosol
entity reference: Calcium

ChemicalStructure/PW C000457
type: ChemicalStructure
structure format: SMILES
structure data: [K+]

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

ReactionCatalysis209023
type: Catalysis
controller: Adenylyl cyclase complex
controlled: 4.6.1.1
control type: ACTIVATION

Guanine nucleotide-binding protein G(i) subunit alpha-1
type: ProteinReference
xref: Reference/UniProt Q45QN2
organism: BiologicalState458

Protein/19766/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-beta regulatory subunit
stoichiometric coefficient: 1.0

cAMP-dependent protein kinase catalytic subunit alpha
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit alpha

Transport10271
type: Transport
participant stoichiometry: Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353 Cytoplasm/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Rattus norvegicus, Extracellular Space to Rattus norvegicus, Cell, Cytoplasm
conversion direction: LEFT_TO_RIGHT

Reference/ChemSpider 559142
type: UnificationXref
id: 559142
db: ChemSpider

Compound/PW C000353/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Compound/PW C000457 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

cAMP
type: SmallMoleculeReference
xref: Reference/PubChem-compound 6076
xref: Reference/HMDB HMDB0000058
xref: Reference/ChEBI CHEBI 17489
xref: Reference/CAS 60-92-4
xref: Reference/BioCyc CAMP
xref: Reference/ChemSpider 5851
xref: Reference/KEGG Compound C00575
chemical formula: C10H12N5O6P
molecular weight: 329.05252
structure: ChemicalStructure/PW C000041

Reference/UniProt P59768
type: UnificationXref
id: P59768
db: UniProt

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
type: Protein
cellular location: Cell Membrane
entity reference: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Reference/UniProt Q08462
type: UnificationXref
id: Q08462
db: UniProt

Compound/PW C000041 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP
stoichiometric coefficient: 1.0

Protein kinase A
type: Complex
component stoichiometry: Protein/19766/Stoichiometry/1.0
component stoichiometry: Protein/19758/Stoichiometry/1.0
component stoichiometry: Protein/19359/Stoichiometry/1.0
component stoichiometry: Protein/19764/Stoichiometry/1.0
component stoichiometry: Protein/19771/Stoichiometry/1.0
component stoichiometry: Protein/19757/Stoichiometry/1.0
component: cAMP-dependent protein kinase catalytic subunit beta
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit alpha
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase type I-beta regulatory subunit

Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Reference/CELL TYPE ONTOLOGY CL 0000187
type: UnificationXref
id: CL:0000187
db: CELL TYPE ONTOLOGY

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

Gγ
type: Protein
cellular location: Cell Membrane
entity reference: Gγ

Reference/KEGG Compound C00575
type: UnificationXref
id: C00575
db: KEGG Compound

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

cAMP-dependent protein kinase type II-beta regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type II-beta regulatory subunit

Muscle Contraction
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway122649
pathway component: SubPathwayInteraction122649
name: SubPathway

Reference/BRENDA TISSUE ONTOLOGY BTO 0000887
type: UnificationXref
id: BTO:0000887
db: BRENDA TISSUE ONTOLOGY

Reference/BRENDA TISSUE ONTOLOGY BTO 0000763
type: UnificationXref
id: BTO:0000763
db: BRENDA TISSUE ONTOLOGY

Unknown
type: Protein
cellular location: Cytosol
entity reference: Unknown

Reference/CAS 50-67-9
type: UnificationXref
id: 50-67-9
db: CAS

Reference/UniProt P12369
type: UnificationXref
id: P12369
db: UniProt

Reference/PubChem-compound 813
type: UnificationXref
id: 813
db: PubChem-compound

ReactionCatalysis209001
type: Catalysis
controller: Myosin light chain kinase 2, skeletal/cardiac muscle
controlled: 2.7.11.18
control type: ACTIVATION

Reference/ChemSpider 5851
type: UnificationXref
id: 5851
db: ChemSpider

ReactionCatalysis209002
type: Catalysis
controller: Unknown
controlled: 3.1.3.16
control type: ACTIVATION

Reference/PubChem-compound 6830
type: UnificationXref
id: 6830
db: PubChem-compound

Guanosine diphosphate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001201
xref: Reference/BioCyc GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE
xref: Reference/CAS 146-91-8
xref: Reference/PubChem-compound 8977
xref: Reference/ChEBI CHEBI 17552
xref: Reference/ChemSpider 8630
xref: Reference/KEGG Compound C00035
chemical formula: C10H15N5O11P2
molecular weight: 443.02432
structure: ChemicalStructure/PW C000936

BiologicalState458
type: BioSource
cell type: Myocyte
xref: Reference/TAXONOMY 10116
name: Rattus norvegicus, Muscle, Myocyte
tissue: Muscle

Reference/ChemSpider 266
type: UnificationXref
id: 266
db: ChemSpider

Rattus norvegicus
type: BioSource
xref: Reference/TAXONOMY 10116

Reference/BioCyc PPI
type: UnificationXref
id: PPI
db: BioCyc

Protein/19766 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-beta regulatory subunit
stoichiometric coefficient: 1.0

Adenylate cyclase type 5
type: ProteinReference
xref: Reference/UniProt Q04400
organism: BiologicalState458

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
type: ProteinReference
xref: Reference/UniProt P62873
organism: BiologicalState458

ProteinComplex/11046 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Protein kinase A
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00238
type: UnificationXref
id: C00238
db: KEGG Compound

L-type calcium channel
type: Complex
component: Unknown
component: Voltage-dependent L-type calcium channel subunit beta-1

Compound/PW C000414 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Guanosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15996
xref: Reference/ChemSpider 6569
xref: Reference/KEGG Compound C00044
xref: Reference/PubChem-compound 6830
xref: Reference/HMDB HMDB0001273
xref: Reference/CAS 86-01-1
xref: Reference/BioCyc GTP
chemical formula: C10H16N5O14P3
molecular weight: 522.99066
structure: ChemicalStructure/PW C000986

Guanosine triphosphate
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Guanosine triphosphate

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

Reaction219648
type: BiochemicalReaction
participant stoichiometry: Protein/19708/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11046/Stoichiometry/1.0
right: Inositol 1,4,5-trisphosphate receptor type 1
spontaneous: false
left: Protein kinase A
name: Protein kinase A → Inositol 1,4,5-trisphosphate receptor
conversion direction: LEFT_TO_RIGHT

Reaction219647
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/16646 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11046 Cytosol/Stoichiometry/1.0
right: Intermediate conductance calcium-activated potassium channel protein 4
spontaneous: false
left: Protein kinase A
name: Protein kinase A → Intermediate conductance calcium-activated potassium channel protein 4
conversion direction: LEFT_TO_RIGHT

Gγ
type: ProteinReference
xref: Reference/UniProt P59768
organism: BiologicalState458

Calcium_Calmodulin (Rattus norvegicus)
type: Complex
component stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19864 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Calmodulin-3
component: Calcium

Adenylyl cyclase complex
type: Complex
cellular location: Cytoplasm
component: Adenylate cyclase type 5
component: Adenylate cyclase type 2
component: Unknown

Reference/GENE ONTOLOGY GO 0005886
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

Protein/19771/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-alpha regulatory subunit
stoichiometric coefficient: 1.0

Reference/CAS 24203-36-9
type: UnificationXref
id: 24203-36-9
db: CAS

Reference/UniProt P22694
type: UnificationXref
id: P22694
db: UniProt

Reference/ChEBI CHEBI 18361
type: UnificationXref
id: CHEBI:18361
db: ChEBI

ChemicalStructure/PW C000936
type: ChemicalStructure
structure format: SMILES

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Reference/HMDB HMDB0000464
type: UnificationXref
id: HMDB0000464
db: HMDB

Myocyte
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000187

Reference/HMDB HMDB0000586
type: UnificationXref
id: HMDB0000586
db: HMDB

Reference/BioCyc GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE
type: UnificationXref
id: GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE
db: BioCyc

Protein/19557 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Myosin light chain 3
stoichiometric coefficient: 1.0

Voltage-dependent L-type calcium channel subunit beta-1
type: Protein
entity reference: Voltage-dependent L-type calcium channel subunit beta-1

Muscle
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000887

Reference/ChEBI CHEBI 17489
type: UnificationXref
id: CHEBI:17489
db: ChEBI

Compound/PW C000170 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Pyrophosphate
stoichiometric coefficient: 1.0

Reference/BioCyc CAMP
type: UnificationXref
id: CAMP
db: BioCyc

Adenosine triphosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Adenosine triphosphate

Reference/UniProt Q45QN2
type: UnificationXref
id: Q45QN2
db: UniProt

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type II-alpha regulatory subunit

Reaction219306
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000936 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000986 Cell Membrane/Stoichiometry/1.0
right: Guanosine triphosphate
spontaneous: false
left: Guanosine diphosphate
name: Guanosine diphosphate → Guanosine triphosphate
conversion direction: LEFT_TO_RIGHT

Intermediate conductance calcium-activated potassium channel protein 4
type: Complex
component stoichiometry: Protein/7758 Cytosol/Stoichiometry/4.0
cellular location: Cytosol
component: Unknown

Reference/KEGG Compound C00013
type: UnificationXref
id: C00013
db: KEGG Compound

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Reference/HMDB HMDB0001201
type: UnificationXref
id: HMDB0001201
db: HMDB

cAMP-dependent protein kinase type II-beta regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type II-beta regulatory subunit

Pyrophosphate
type: SmallMoleculeReference
xref: Reference/PubChem-compound 644102
xref: Reference/BioCyc PPI
xref: Reference/ChemSpider 559142
xref: Reference/ChEBI CHEBI 18361
xref: Reference/HMDB HMDB0000250
xref: Reference/CAS 14000-31-8
xref: Reference/KEGG Compound C00013
chemical formula: H4O7P2
molecular weight: 177.94322
structure: ChemicalStructure/PW C000170

Serotonin_5-hydroxytryptamine receptor 1A (Rattus norvegicus)
type: Complex
component stoichiometry: Compound/PW C000178 Cell Membrane/Stoichiometry/1.0
component stoichiometry: Protein/21274 Cell Membrane/Stoichiometry/1.0
cellular location: Cell Membrane
component: 5-hydroxytryptamine receptor 1A
component: Serotonin

4.6.1.1
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000041 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000170 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
right: cAMP
right: Pyrophosphate
spontaneous: false
EC number: 4.6.1.1
left: Adenosine triphosphate
name: Adenosine triphosphate → Pyrophosphate + cAMP
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

Reference/CAS 14127-61-8
type: UnificationXref
id: 14127-61-8
db: CAS

ChemicalStructure/PW C000353
type: ChemicalStructure
structure format: SMILES
structure data: [Ca++]

Serotonin
type: SmallMoleculeReference
xref: Reference/CAS 50-67-9
xref: Reference/HMDB HMDB0000259
xref: Reference/ChemSpider 5013
xref: Reference/KEGG Compound C00780
xref: Reference/ChEBI CHEBI 28790
xref: Reference/PubChem-compound 5202
chemical formula: C10H12N2O
molecular weight: 176.09496
structure: ChemicalStructure/PW C000178

cAMP-dependent protein kinase catalytic subunit beta
type: ProteinReference
xref: Reference/UniProt P68182
organism: BiologicalState1512

Adenylate cyclase type 2
type: Protein
cellular location: Cytoplasm
entity reference: Adenylate cyclase type 2

Protein/19758/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-alpha regulatory subunit
stoichiometric coefficient: 1.0

Protein/21274 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5-hydroxytryptamine receptor 1A
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 15996
type: UnificationXref
id: CHEBI:15996
db: ChEBI

Calcium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Calcium

Myosin light chain 3
type: Protein
cellular location: Cytosol
entity reference: Myosin light chain 3
name: SubPathwayInput

Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

Guanine nucleotide-binding protein G(i) subunit alpha-1
type: Protein
cellular location: Cytoplasm
entity reference: Guanine nucleotide-binding protein G(i) subunit alpha-1

cAMP-dependent protein kinase type I-beta regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type I-beta regulatory subunit

Adenosine triphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Adenosine triphosphate

cAMP-dependent protein kinase catalytic subunit alpha
type: Protein
entity reference: cAMP-dependent protein kinase catalytic subunit alpha

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type I-alpha regulatory subunit

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

Reference/ChEBI CHEBI 29108
type: UnificationXref
id: CHEBI:29108
db: ChEBI

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

Protein/19708/Stoichiometry/1.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate receptor type 1
stoichiometric coefficient: 1.0

Reference/CAS 60-92-4
type: UnificationXref
id: 60-92-4
db: CAS

Reference/UniProt P54283
type: UnificationXref
id: P54283
db: UniProt

Reference/ChEBI CHEBI 29103
type: UnificationXref
id: CHEBI:29103
db: ChEBI

cAMP
type: SmallMolecule
cellular location: Cytoplasm
entity reference: cAMP

Guanosine diphosphate
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Guanosine diphosphate

Reference/TAXONOMY 10116
type: UnificationXref
id: 10116
db: TAXONOMY

Voltage-dependent L-type calcium channel subunit beta-1
type: ProteinReference
xref: Reference/UniProt P54283

BoundComplex/649 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium_Calmodulin (Rattus norvegicus)
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00035
type: UnificationXref
id: C00035
db: KEGG Compound

Reference/HMDB HMDB0000259
type: UnificationXref
id: HMDB0000259
db: HMDB

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: ProteinReference
xref: Reference/UniProt Q8K1M2
organism: BiologicalState1512

ChemicalStructure/PW C000178
type: ChemicalStructure
structure format: SMILES
structure data: NCCC1=CNC2=C1C=C(O)C=C2

Calcium
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Calcium

Protein/19757 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit alpha
stoichiometric coefficient: 1.0

SubPathwayInteraction122650
type: Interaction
name: SubPathwayReaction
name: SubPathway122650Reaction
participant: Myosin light chain 3

Reference/HMDB HMDB0000250
type: UnificationXref
id: HMDB0000250
db: HMDB

ChemicalStructure/PW C000170
type: ChemicalStructure
structure format: SMILES
structure data: OP(O)(=O)OP(O)(O)=O

Potassium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Potassium

Reference/CAS 14000-31-8
type: UnificationXref
id: 14000-31-8
db: CAS

Reference/SMPDB SMP0127687
type: UnificationXref
id: SMP0127687
db: SMPDB

Myosin light chain kinase 2, skeletal/cardiac muscle
type: Protein
cellular location: Cytosol
entity reference: Myosin light chain kinase 2, skeletal/cardiac muscle

Protein/19359 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit beta
stoichiometric coefficient: 1.0

Compound/PW C000353 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Reference/UniProt P09456
type: UnificationXref
id: P09456
db: UniProt

Reference/KEGG Compound C00044
type: UnificationXref
id: C00044
db: KEGG Compound

Unknown
type: Protein
entity reference: Unknown

Reference/BioCyc K+
type: UnificationXref
id: K%2b
db: BioCyc

Reference/PubChem-compound 5202
type: UnificationXref
id: 5202
db: PubChem-compound

SubPathwayInteraction122649
type: Interaction
name: SubPathway122649Reaction
name: SubPathwayReaction
participant: Myosin light chain 3

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

Lung
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000763

Protein kinase A
type: Complex
component stoichiometry: Protein/19359 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19771 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19758 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19757 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19764 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19766 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit alpha
component: cAMP-dependent protein kinase type I-beta regulatory subunit
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit beta

PKA complex
type: Complex
cellular location: Cytosol
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit alpha
component: cAMP-dependent protein kinase type I-beta regulatory subunit
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit beta

Compound/PW C000986 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Guanosine triphosphate
stoichiometric coefficient: 1.0

3.1.3.16
type: BiochemicalReaction
participant stoichiometry: Protein/19557 Cytosol/Stoichiometry/1.0
right: Myosin light chain 3
spontaneous: false
EC number: 3.1.3.16
left: Myosin light chain 3
name: Myosin LC-P → Myosin light chain 3
conversion direction: LEFT_TO_RIGHT

Guanine nucleotide-binding protein G(i) subunit alpha-1
type: Protein
cellular location: Cell Membrane
entity reference: Guanine nucleotide-binding protein G(i) subunit alpha-1

2.7.11.18
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000414 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/19557 Cytosol/Stoichiometry/1.0
right: Myosin light chain 3
spontaneous: false
EC number: 2.7.11.18
left: Adenosine triphosphate
left: Myosin light chain 3
name: Adenosine triphosphate + Myosin light chain 3 → Myosin LC-P
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

G protein complex
type: Complex
cellular location: Cell Membrane
component: Guanine nucleotide-binding protein G(i) subunit alpha-1
component: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
component: Gγ

Potassium
type: SmallMolecule
cellular location: Cytosol
entity reference: Potassium

Reference/UniProt Q62869
type: UnificationXref
id: Q62869
db: UniProt

Interaction5300
type: MolecularInteraction
interaction type: direct_interaction
name: Calcium + Calmodulin (Rattus norvegicus) activates Myosin light chain kinase, smooth muscle
participant: Calcium_Calmodulin (Rattus norvegicus)
participant: Myosin light chain kinase 2, skeletal/cardiac muscle

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type II-alpha regulatory subunit

Reference/ChemSpider 791
type: UnificationXref
id: 791
db: ChemSpider

Myosin light chain kinase 2, skeletal/cardiac muscle
type: ProteinReference
xref: Reference/UniProt P20689
organism: BiologicalState1512

Myosin light chain 3
type: ProteinReference
xref: Reference/UniProt P16409
organism: BiologicalState1512

Protein/19764 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-beta regulatory subunit
stoichiometric coefficient: 1.0

ProteinComplex/16646 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Intermediate conductance calcium-activated potassium channel protein 4
stoichiometric coefficient: 1.0

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

Interaction5415
type: MolecularInteraction
interaction type: direct_interaction
name: G protein complex inhibits Adenylyl cyclase complex
participant: Adenylyl cyclase complex
participant: G protein complex

Reaction219630
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: BoundComplex/649 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/19864 Cytosol/Stoichiometry/1.0
right: Calcium_Calmodulin (Rattus norvegicus)
spontaneous: false
left: Calmodulin-3
left: Calcium
name: Calcium + Calmodulin → Calcium + Calmodulin (Rattus norvegicus)
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 8977
type: UnificationXref
id: 8977
db: PubChem-compound

Adenylate cyclase type 5
type: Protein
cellular location: Cytoplasm
entity reference: Adenylate cyclase type 5

Interaction5414
type: MolecularInteraction
interaction type: direct_interaction
name: G protein complex activates 5-hydroxytryptamine receptor 1A
participant: G protein complex
participant: 5-hydroxytryptamine receptor 1A

Protein/7758 Cytosol/Stoichiometry/4.0
type: Stoichiometry
physical entity: Unknown
stoichiometric coefficient: 4.0

Unknown
type: Protein
cellular location: Cytoplasm
entity reference: Unknown

Pyrophosphate
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Pyrophosphate

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type I-alpha regulatory subunit

Calmodulin-3
type: Protein
cellular location: Cytosol
entity reference: Calmodulin-3

ChemicalStructure/PW C000041
type: ChemicalStructure
structure format: SMILES

Reference/UniProt P68182
type: UnificationXref
id: P68182
db: UniProt

Protein/19771 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-alpha regulatory subunit
stoichiometric coefficient: 1.0

Reference/UniProt P62873
type: UnificationXref
id: P62873
db: UniProt

Protein/19758 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-alpha regulatory subunit
stoichiometric coefficient: 1.0

Interaction5566
type: MolecularInteraction
interaction type: direct_interaction
name: G protein complex activates Serotonin + 5-hydroxytryptamine receptor 1A (Rattus norvegicus)
participant: G protein complex
participant: Serotonin_5-hydroxytryptamine receptor 1A (Rattus norvegicus)

Reference/KEGG Compound C00076
type: UnificationXref
id: C00076
db: KEGG Compound

Adenylate cyclase type 2
type: ProteinReference
xref: Reference/UniProt Q08462
organism: BiologicalState458

Reference/ChemSpider 8630
type: UnificationXref
id: 8630
db: ChemSpider

Interaction5320
type: MolecularInteraction
interaction type: direct_interaction
name: cAMP activates PKA complex
participant: PKA complex
participant: cAMP

Protein/19864 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calmodulin-3
stoichiometric coefficient: 1.0

Reference/UniProt P20689
type: UnificationXref
id: P20689
db: UniProt

Reference/BioCyc CA+2
type: UnificationXref
id: CA%2b2
db: BioCyc

Reference/HMDB HMDB0000058
type: UnificationXref
id: HMDB0000058
db: HMDB

Reference/UniProt P19327
type: UnificationXref
id: P19327
db: UniProt

Inositol 1,4,5-trisphosphate receptor type 1
type: Protein
entity reference: Inositol 1,4,5-trisphosphate receptor type 1

Calcium
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000464
xref: Reference/BioCyc CA+2
xref: Reference/ChemSpider 266
xref: Reference/ChEBI CHEBI 29108
xref: Reference/CAS 14127-61-8
xref: Reference/KEGG Compound C00076
xref: Reference/PubChem-compound 271
chemical formula: Ca
molecular weight: 39.96259
structure: ChemicalStructure/PW C000353

TransportCatalysis6011
type: Catalysis
controller: L-type calcium channel
controlled: Transport16266
control type: ACTIVATION

BiologicalState1512
type: BioSource
cell type: Myocyte
xref: Reference/TAXONOMY 10116
name: Rattus norvegicus, Lung, Myocyte
tissue: Lung

Protein/19757/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit alpha
stoichiometric coefficient: 1.0

Cell Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005886

Reference/ChemSpider 6569
type: UnificationXref
id: 6569
db: ChemSpider

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Reference/PathWhiz PW129306
type: UnificationXref
id: PW129306
db: PathWhiz

Reference/PubChem-compound 271
type: UnificationXref
id: 271
db: PubChem-compound

Reference/ChEBI CHEBI 17552
type: UnificationXref
id: CHEBI:17552
db: ChEBI

Reference/BioCyc GTP
type: UnificationXref
id: GTP
db: BioCyc

Gi Serotonergic Smooth Muscle Relaxation
type: Pathway
pathway order: PathwayEnzymeReaction/2552769
pathway order: PathwayEnzymeReaction/2552767
pathway order: PathwayEnzymeReaction/2552768
pathway order: PathwayInteraction/5566
pathway order: PathwayEnzymeReaction/2552765
pathway order: PathwayEnzymeReaction/2552766
pathway order: PathwayEnzymeReaction/2552764
pathway order: PathwaySubPathway/122649
pathway order: PathwayEnzymeReaction/2552770
pathway order: PathwayTransport/2552772
pathway order: PathwayTransport/2552773
pathway order: PathwaySubPathway/122650
pathway order: PathwayTransport/2552771
pathway order: PathwayInteraction/5415
pathway order: PathwayInteraction/5414
pathway order: PathwayInteraction/5300
pathway order: PathwayInteraction/5320
pathway component: 3.1.3.16
pathway component: 2.7.11.18
pathway component: Interaction5566
pathway component: Interaction5415
pathway component: ReactionCatalysis209001
pathway component: Reaction219630
pathway component: ReactionCatalysis209023
pathway component: Interaction5414
pathway component: ReactionCatalysis209002
pathway component: Reaction219648
pathway component: Reaction219306
pathway component: Reaction219647
pathway component: Interaction5320
pathway component: Interaction5300
pathway component: Muscle Relaxation
pathway component: 4.6.1.1
pathway component: Transport16266
pathway component: Muscle Contraction
pathway component: Transport10271
pathway component: Transport16274
pathway component: TransportCatalysis6011
xref: Reference/SMPDB SMP0127687
xref: Reference/PathWhiz PW129306
organism: Rattus norvegicus

Compound/PW C000414 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Muscle Relaxation
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway122650
pathway component: SubPathwayInteraction122650
name: SubPathway

cAMP-dependent protein kinase type I-beta regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type I-beta regulatory subunit

Calmodulin-3
type: ProteinReference
xref: Reference/UniProt P0DP31
organism: BiologicalState1512

Serotonin
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Serotonin

Reference/ChEBI CHEBI 28790
type: UnificationXref
id: CHEBI:28790
db: ChEBI

Reference/HMDB HMDB0001273
type: UnificationXref
id: HMDB0001273
db: HMDB

Reference/UniProt P81377
type: UnificationXref
id: P81377
db: UniProt

Reference/PubChem-compound 644102
type: UnificationXref
id: 644102
db: PubChem-compound

Compound/PW C000178 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Serotonin
stoichiometric coefficient: 1.0

5-hydroxytryptamine receptor 1A
type: Protein
cellular location: Cell Membrane
entity reference: 5-hydroxytryptamine receptor 1A

Gγ
type: Protein
cellular location: Cytoplasm
entity reference: Gγ

Unknown
type: ProteinReference
organism: BiologicalState1512

ChemicalStructure/PW C000986
type: ChemicalStructure
structure format: SMILES

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit beta

5-hydroxytryptamine receptor 1A
type: ProteinReference
xref: Reference/UniProt P19327
organism: BiologicalState458

Calcium
type: SmallMolecule
entity reference: Calcium

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: ProteinReference
xref: Reference/UniProt P09456
organism: BiologicalState1512

Reference/CAS 86-01-1
type: UnificationXref
id: 86-01-1
db: CAS

cAMP-dependent protein kinase catalytic subunit alpha
type: ProteinReference
xref: Reference/UniProt Q6UA68
organism: BiologicalState1512

Reference/PubChem-compound 6076
type: UnificationXref
id: 6076
db: PubChem-compound

Potassium
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00238
xref: Reference/HMDB HMDB0000586
xref: Reference/PubChem-compound 813
xref: Reference/ChemSpider 791
xref: Reference/CAS 24203-36-9
xref: Reference/BioCyc K+
xref: Reference/ChEBI CHEBI 29103
chemical formula: K
molecular weight: 38.963707
structure: ChemicalStructure/PW C000457

Protein/19359/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit beta
stoichiometric coefficient: 1.0

Reference/ChemSpider 5013
type: UnificationXref
id: 5013
db: ChemSpider

Reference/KEGG Compound C00780
type: UnificationXref
id: C00780
db: KEGG Compound

Reference/UniProt P16409
type: UnificationXref
id: P16409
db: UniProt

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
entity reference: cAMP-dependent protein kinase catalytic subunit beta

Reference/UniProt Q04400
type: UnificationXref
id: Q04400
db: UniProt

Compound/PW C000936 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Guanosine diphosphate
stoichiometric coefficient: 1.0

Reference/CAS 146-91-8
type: UnificationXref
id: 146-91-8
db: CAS

cAMP-dependent protein kinase type I-beta regulatory subunit
type: ProteinReference
xref: Reference/UniProt P81377
organism: BiologicalState1512

Transport16274
type: Transport
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Cytosol/Stoichiometry/1.0
right: Potassium
spontaneous: false
left: Potassium
name: Potassium (→) Transport: Rattus norvegicus, Muscle, Myocyte, Cytosol to Rattus norvegicus, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Compound/PW C000353 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

cAMP-dependent protein kinase type II-beta regulatory subunit
type: ProteinReference
xref: Reference/UniProt P12369
organism: BiologicalState1512

5-hydroxytryptamine receptor 1A
type: Protein
cellular location: Cytoplasm
entity reference: 5-hydroxytryptamine receptor 1A