FAD-dependent pyridine nucleotide-disulfide oxi
2-oxopent-4-enoate + Water ↔ 4-hydroxy-2-oxopentanoate
2-Hydroxy-6-oxo-7-methylocta-2,4-dienoate + Water ↔ 2-oxopent-4-enoate + Isobutyric acid
2-Hydroxy-3-carboxy-6-oxo-7-methylocta-2,4-dienoate ↔ 2-Hydroxy-6-oxo-7-methylocta-2,4-dienoate + Carbon dioxide
4-hydroxy-2-oxopentanoate ↔ Acetaldehyde + Pyruvic acid
Acetaldehyde + Coenzyme A + NAD ↔ Acetyl-CoA + Hydrogen Ion + NADH
NAD + cis-2,3-Dihydroxy-2,3-dihydro-p-cumate ↔ 2,3-Dihydroxy-p-cumate + Hydrogen Ion + NADH
2,3-Dihydroxy-p-cumate + Oxygen ↔ 2-Hydroxy-3-carboxy-6-oxo-7-methylocta-2,4-dienoate
2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase
4-Isopropylbenzoic acid + Hydrogen Ion + NADH + Oxygen ↔ NAD + cis-2,3-Dihydroxy-2,3-dihydro-p-cumate
4-Isopropylbenzyl alcohol + NAD ↔ Cuminaldehyde + Hydrogen Ion + NADH
Cuminaldehyde + NAD + Water ↔ 4-Isopropylbenzoic acid + Hydrogen Ion + NADH
Glyoxalase/bleomycin resistance protein/dioxygen
Class II aldolase/adducin family protein
2 Hydrogen Ion + Oxygen + 2 Reduced ferredoxin + p-Cymene ↔ 4-Isopropylbenzyl alcohol + 2 Oxidized ferredoxin + Water
p-Cymene (→) Transport: Pseudomonas putida DOT-T1E, Cell, Extracellular Space to Pseudomonas putida DOT-T1E, Cell, Cytoplasm