Hydrogen Ion + L-Glutamic acid 5-phosphate + NADPH → L-Glutamic γ-semialdehyde + NADP + Phosphate
Glutamate-aspartate periplasmic-binding protein
Glutamate ABC transporter, ATP-binding protein GluA
L-Glutamic γ-semialdehyde + NAD + Water → 2 Hydrogen Ion + L-Glutamic acid + NADH
Adenosine triphosphate + L-Glutamic acid → Adenosine diphosphate + L-Glutamic acid 5-phosphate
1-Pyrroline-5-carboxylic acid + Hydrogen Ion + NADPH → L-Proline + NADP
L-Proline + Ubiquinone-1 → 1-Pyrroline-5-carboxylic acid + Hydrogen Ion + Ubiquinol-1
Adenosine triphosphate + L-Glutamic acid + Water → Adenosine diphosphate + Hydrogen Ion + L-Glutamic acid + Phosphate
Bifunctional aspartokinase/homoserine dehydrogenase
1-Pyrroline-5-carboxylic acid + Hydrogen Ion + Water → L-Glutamic γ-semialdehyde
L-Glutamic γ-semialdehyde → 1-Pyrroline-5-carboxylic acid + Hydrogen Ion + Water