2-iminobutanoate + Hydrogen Ion + Water → 2-Ketobutyric acid + Ammonium
Adenosine triphosphate + L-Isoleucine + Water → Adenosine diphosphate + Hydrogen Ion + L-Isoleucine + Phosphate
(2Z)-2-aminobut-2-enoate → 2-iminobutanoate
Branched-chain amino acid transporter permease subunit LivH
(S)-2-Aceto-2-hydroxybutanoic acid + Hydrogen Ion + NADPH → (R) 2,3-Dihydroxy-3-methylvalerate + NADP
(R) 2,3-Dihydroxy-3-methylvalerate → 3-Methyl-2-oxovaleric acid + Water
3-Methyl-2-oxovaleric acid + L-Glutamic acid → L-Isoleucine + Oxoglutaric acid
ATP-dependent Clp protease ATP-binding subunit ClpA
3-Methyl-2-oxovaleric acid ↔ 2-Keto-3-methyl-valerate
High-affinity branched-chain amino acid transport ATP-binding protein LivG
High-affinity branched-chain amino acid transport ATP-binding protein
Branched-chain amino acid ABC transporter, permease protein
acetohydroxybutanoate synthase / acetolactate synthase
L-Threonine → (2Z)-2-aminobut-2-enoate + Hydrogen Ion + Water
branched-chain amino-acid aminotransferase
2-Ketobutyric acid + Hydrogen Ion + Pyruvic acid → (S)-2-Aceto-2-hydroxybutanoic acid + Carbon dioxide
acetohydroxybutanoate synthase / acetolactate synthase 2
acetolactate synthase / acetohydroxybutanoate synthase 3
Branched-chain amino acid transport system carrier protein
L-Isoleucine (→), Sodium (→) Transport: Providencia alcalifaciens DSM 30120, Periplasmic Space to Providencia alcalifaciens DSM 30120, Cytosol
branched chain amino acid ABC transporter