Compound/Generic227 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: oxidized electron acceptor
stoichiometric coefficient: 1.0

Reference/TAXONOMY 3702
type: UnificationXref
id: 3702
db: TAXONOMY

ChemicalStructure/PW C000448
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CS)C(O)=O

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Reference/ChEBI CHEBI 71302
type: UnificationXref
id: CHEBI:71302
db: ChEBI

Molybdopterin-AMP
type: SmallMolecule
entity reference: Molybdopterin-AMP

Reference/BioCyc CYS
type: UnificationXref
id: CYS
db: BioCyc

Reference/HMDB HMDB0001983
type: UnificationXref
id: HMDB0001983
db: HMDB

Adenosine monophosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Adenosine monophosphate

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Guanosine triphosphate
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: Guanosine triphosphate
name: SubPathwayOutput

Pyrophosphate
type: SmallMolecule
entity reference: Pyrophosphate

Adenosine monophosphate
type: SmallMoleculeReference
xref: Reference/CAS 61-19-8
xref: Reference/ChemSpider 5858
xref: Reference/HMDB HMDB0000045
xref: Reference/BioCyc AMP
xref: Reference/ChEBI CHEBI 16027
xref: Reference/PubChem-compound 6083
xref: Reference/KEGG Compound C00020
chemical formula: C10H14N5O7P
molecular weight: 347.06308
structure: ChemicalStructure/PW C000032

Reference/HMDB HMDB0001185
type: UnificationXref
id: HMDB0001185
db: HMDB

Compound/PW C040034/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

molybdenum cofactor sulfurtransferase
type: ProteinReference
xref: Reference/UniProt Q9C5X8
organism: Arabidopsis thaliana

Reference/ChEBI CHEBI 16027
type: UnificationXref
id: CHEBI:16027
db: ChEBI

Reference/ChemSpider 4883416
type: UnificationXref
id: 4883416
db: ChemSpider

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

Reference/PubChem-compound 6137
type: UnificationXref
id: 6137
db: PubChem-compound

2.8.1.9
type: BiochemicalReaction
participant stoichiometry: Compound/Generic227 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C057878 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000105 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/Generic237 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040077 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000448 Cytosol/Stoichiometry/1.0
right: Thio-molybdenum cofactor
right: L-Alanine
right: oxidized electron acceptor
right: Water
spontaneous: false
EC number: 2.8.1.9
left: L-Cysteine
left: reduced electron acceptor
left: molybdenum cofactor (Moco)
name: L-Cysteine + molybdenum cofactor (Moco) + reduced electron acceptor → L-Alanine + Thio-molybdenum cofactor + Water + oxidized electron acceptor
conversion direction: LEFT_TO_RIGHT

Compound/PW C000414/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Molybdopterin
type: SmallMolecule
entity reference: Molybdopterin

Reference/ChemSpider 559142
type: UnificationXref
id: 559142
db: ChemSpider

Molybdopterin
type: SmallMoleculeReference
xref: Reference/ChemSpider 4883416
xref: Reference/KEGG Compound C05924
xref: Reference/BioCyc CPD0-1882
xref: Reference/ChEBI CHEBI 21437
xref: Reference/HMDB HMDB0002206
xref: Reference/PubChem-compound 23304237
xref: Reference/CAS 73508-07-3
chemical formula: C10H16MoN5O8PS2
molecular weight: 526.9232
structure: ChemicalStructure/PW C001491

ReactionCatalysis37584
type: Catalysis
controller: molybdenum insertase
controlled: Reaction47438
control type: ACTIVATION

S-Adenosylmethionine
type: SmallMoleculeReference
xref: Reference/PubChem-compound 24762165
xref: Reference/KEGG Compound C00019
xref: Reference/ChemSpider 31983
xref: Reference/ChEBI CHEBI 15414
xref: Reference/CAS 485-80-3
xref: Reference/BioCyc S-ADENOSYLMETHIONINE
xref: Reference/HMDB HMDB0001185
chemical formula: C15H23N6O5S
molecular weight: 399.14505
structure: ChemicalStructure/PW C000921

Compound/PW C000986 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: Guanosine triphosphate
stoichiometric coefficient: 1.0

molybdopterin synthase
type: Complex
component stoichiometry: Protein/11487 Cytosol/Stoichiometry/2.0
component stoichiometry: Protein/11488 Cytosol/Stoichiometry/2.0
cellular location: Cytosol
component: molybdopterin synthase small subunit
component: molybdopterin synthase large subunit

Reference/PubChem-compound 439182
type: UnificationXref
id: 439182
db: PubChem-compound

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

Reference/PubChem-compound 1038
type: UnificationXref
id: 1038
db: PubChem-compound

ReactionCatalysis37581
type: Catalysis
controller: cPMP synthase
controlled: 4.6.1.17
control type: ACTIVATION

ReactionCatalysis37580
type: Catalysis
controller: GTP 3',8-cyclase
controlled: 4.1.99.22
control type: ACTIVATION

ReactionCatalysis37583
type: Catalysis
controller: molybdenum insertase
controlled: 2.7.7.75
control type: ACTIVATION

Reference/ChEBI CHEBI 17561
type: UnificationXref
id: CHEBI:17561
db: ChEBI

ChemicalStructure/PW C000548
type: ChemicalStructure
structure format: SMILES
structure data: CSCC[C@H](N)C(O)=O

ReactionCatalysis37582
type: Catalysis
controller: molybdopterin synthase
controlled: 2.8.1.12
control type: ACTIVATION

Molybdopterin-AMP
type: SmallMolecule
cellular location: Cytosol
entity reference: Molybdopterin-AMP

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Compound/PW C007807/Stoichiometry/1.0
type: Stoichiometry
physical entity: Molybdopterin-AMP
stoichiometric coefficient: 1.0

molybdenum cofactor (Moco)
type: SmallMolecule
cellular location: Cytosol
entity reference: molybdenum cofactor (Moco)

Cyclic pyranopterin monophosphate
type: SmallMoleculeReference
xref: Reference/ChemSpider 17221217
xref: Reference/HMDB HMDB0059639
xref: Reference/ChEBI CHEBI 60210
xref: Reference/KEGG Compound C18239
xref: Reference/PubChem-compound 16061579
chemical formula: C10H14N5O8P
molecular weight: 363.058
structure: ChemicalStructure/PW C040076

Reference/ChemSpider 5858
type: UnificationXref
id: 5858
db: ChemSpider

Thio-molybdenum cofactor
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0059640
chemical formula: C10H18MoN5O7PS3
molecular weight: 544.916
structure: ChemicalStructure/PW C040077

Thio-Molybdenum Cofactor Biosynthesis
type: Pathway
pathway order: PathwaySubPathway/4447
pathway order: PathwayEnzymeReaction/224341
pathway order: PathwayEnzymeReaction/224340
pathway order: PathwayTransport/224342
pathway order: PathwayEnzymeReaction/224343
pathway order: PathwayEnzymeReaction/224339
pathway order: PathwayEnzymeReaction/224338
pathway order: PathwayEnzymeReaction/224337
pathway component: ReactionCatalysis37584
pathway component: 4.1.99.22
pathway component: 4.6.1.17
pathway component: ReactionCatalysis37581
pathway component: Purine Metabolism
pathway component: ReactionCatalysis37580
pathway component: 2.8.1.12
pathway component: 2.7.7.75
pathway component: ReactionCatalysis37583
pathway component: 2.8.1.9
pathway component: ReactionCatalysis37582
pathway component: Reaction47438
pathway component: Transport888
pathway component: ReactionCatalysis37659
pathway component: TransportCatalysis788
xref: Reference/PathWhiz PW012907
xref: Reference/SMPDB SMP0012046
organism: Arabidopsis thaliana

Compound/PW C001329 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: 5'-Deoxyadenosine
stoichiometric coefficient: 1.0

L-Methionine
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: L-Methionine

Reference/ChemSpider 5735
type: UnificationXref
id: 5735
db: ChemSpider

Reference/CAS 485-80-3
type: UnificationXref
id: 485-80-3
db: CAS

Reference/PubChem-compound 6830
type: UnificationXref
id: 6830
db: PubChem-compound

Reference/PubChem-compound 5862
type: UnificationXref
id: 5862
db: PubChem-compound

Reference/ChemSpider 1010
type: UnificationXref
id: 1010
db: ChemSpider

Cyclic pyranopterin monophosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Cyclic pyranopterin monophosphate

3',8-cH2GTP
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: 3',8-cH2GTP

Reference/BioCyc PPI
type: UnificationXref
id: PPI
db: BioCyc

Compound/PW C007805 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Molybdate
stoichiometric coefficient: 1.0

Guanosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15996
xref: Reference/ChemSpider 6569
xref: Reference/KEGG Compound C00044
xref: Reference/PubChem-compound 6830
xref: Reference/HMDB HMDB0001273
xref: Reference/CAS 86-01-1
xref: Reference/BioCyc GTP
chemical formula: C10H16N5O14P3
molecular weight: 522.99066
structure: ChemicalStructure/PW C000986

Reference/KEGG Compound C05924
type: UnificationXref
id: C05924
db: KEGG Compound

Hydrogen Ion
type: SmallMolecule
entity reference: Hydrogen Ion

Reference/HMDB HMDB0059640
type: UnificationXref
id: HMDB0059640
db: HMDB

Reference/PubChem-compound 23304237
type: UnificationXref
id: 23304237
db: PubChem-compound

Reference/ChEBI CHEBI 16977
type: UnificationXref
id: CHEBI:16977
db: ChEBI

ChemicalStructure/PW C000921
type: ChemicalStructure
structure format: SMILES

Reference/BioCyc CH33ADO
type: UnificationXref
id: CH33ADO
db: BioCyc

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

Adenosine triphosphate
type: SmallMolecule
entity reference: Adenosine triphosphate

Reference/BioCyc CPD-19179
type: UnificationXref
id: CPD-19179
db: BioCyc

Reference/HMDB HMDB0000574
type: UnificationXref
id: HMDB0000574
db: HMDB

Reference/HMDB HMDB0000696
type: UnificationXref
id: HMDB0000696
db: HMDB

Compound/PW C001491 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Molybdopterin
stoichiometric coefficient: 1.0

Reference/ChemSpider 388325
type: UnificationXref
id: 388325
db: ChemSpider

Reference/CAS 4754-39-6
type: UnificationXref
id: 4754-39-6
db: CAS

Reference/ChEBI CHEBI 15414
type: UnificationXref
id: CHEBI:15414
db: ChEBI

Hydrogen Ion
type: SmallMolecule
cellular location: Cytosol
entity reference: Hydrogen Ion

Reference/ChemSpider 17221217
type: UnificationXref
id: 17221217
db: ChemSpider

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Reference/BioCyc L-ALPHA-ALANINE
type: UnificationXref
id: L-ALPHA-ALANINE
db: BioCyc

Reference/ChEBI CHEBI 18361
type: UnificationXref
id: CHEBI:18361
db: ChEBI

Reference/CAS 56-41-7
type: UnificationXref
id: 56-41-7
db: CAS

Compound/PW C057876 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: 3',8-cH2GTP
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Reference/KEGG Compound C06232
type: UnificationXref
id: C06232
db: KEGG Compound

Reference/GENE ONTOLOGY GO 0005759
type: UnificationXref
id: GO:0005759
db: GENE ONTOLOGY

Pyrophosphate
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: Pyrophosphate

5'-Deoxyadenosine
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: 5'-Deoxyadenosine

Reference/KEGG Compound C00019
type: UnificationXref
id: C00019
db: KEGG Compound

Molybdate
type: SmallMolecule
cellular location: Cytosol
entity reference: Molybdate

Compound/PW C040034 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Protein/11488 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: molybdopterin synthase small subunit
stoichiometric coefficient: 2.0

Reference/CAS 7782-91-4
type: UnificationXref
id: 7782-91-4
db: CAS

Reference/BioCyc S-ADENOSYLMETHIONINE
type: UnificationXref
id: S-ADENOSYLMETHIONINE
db: BioCyc

ChemicalStructure/PW C000105
type: ChemicalStructure
structure format: SMILES
structure data: C[C@H](N)C(O)=O

ABC transporter of the mitochondrion 3
type: Protein
entity reference: ABC transporter of the mitochondrion 3

Reference/PubChem-compound 5950
type: UnificationXref
id: 5950
db: PubChem-compound

Hydrogen Ion
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: Hydrogen Ion

Reference/KEGG Compound C00013
type: UnificationXref
id: C00013
db: KEGG Compound

Pyrophosphate
type: SmallMoleculeReference
xref: Reference/PubChem-compound 644102
xref: Reference/BioCyc PPI
xref: Reference/ChemSpider 559142
xref: Reference/ChEBI CHEBI 18361
xref: Reference/HMDB HMDB0000250
xref: Reference/CAS 14000-31-8
xref: Reference/KEGG Compound C00013
chemical formula: H4O7P2
molecular weight: 177.94322
structure: ChemicalStructure/PW C000170

molybdenum insertase
type: Protein
entity reference: molybdenum insertase

Protein/11487 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: molybdopterin synthase large subunit
stoichiometric coefficient: 2.0

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

ReactionCatalysis37659
type: Catalysis
controller: molybdenum cofactor sulfurtransferase
controlled: 2.8.1.9
control type: ACTIVATION

Cyclic pyranopterin monophosphate
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: Cyclic pyranopterin monophosphate

Reference/UniProt Q9S7A3
type: UnificationXref
id: Q9S7A3
db: UniProt

Hydrogen Ion
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00080
xref: Reference/ChEBI CHEBI 15378
xref: Reference/HMDB HMDB0059597
xref: Reference/PubChem-compound 1038
xref: Reference/ChemSpider 1010
chemical formula: H
molecular weight: 1.007825
structure: ChemicalStructure/PW C040034

Reference/ChEBI CHEBI 15996
type: UnificationXref
id: CHEBI:15996
db: ChEBI

Reference/SMPDB SMP0012046
type: UnificationXref
id: SMP0012046
db: SMPDB

Molybdate
type: SmallMoleculeReference
xref: Reference/KEGG Compound C06232
xref: Reference/ChEBI CHEBI 25371
xref: Reference/ChemSpider 74188
xref: Reference/CAS 7782-91-4
xref: Reference/HMDB HMDB0012260
xref: Reference/PubChem-compound 82208
chemical formula: H2MoO4
molecular weight: 163.89961
structure: ChemicalStructure/PW C007805

Reference/BioCyc MET
type: UnificationXref
id: MET
db: BioCyc

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

Reference/KEGG Compound C00020
type: UnificationXref
id: C00020
db: KEGG Compound

Reference/ChemSpider 5653
type: UnificationXref
id: 5653
db: ChemSpider

Reference/HMDB HMDB0059639
type: UnificationXref
id: HMDB0059639
db: HMDB

Reference/UniProt O22827
type: UnificationXref
id: O22827
db: UniProt

ChemicalStructure/PW C001329
type: ChemicalStructure
structure format: SMILES

Molybdopterin-AMP
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0012262
xref: Reference/PubChem-compound 25245677
chemical formula: C20H26N10O12P2S2
molecular weight: 724.0648
structure: ChemicalStructure/PW C007807

molybdopterin synthase large subunit
type: Protein
cellular location: Cytosol
entity reference: molybdopterin synthase large subunit

oxidized electron acceptor
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: oxidized electron acceptor

Reference/BioCyc CPD0-1882
type: UnificationXref
id: CPD0-1882
db: BioCyc

L-Methionine
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000696
xref: Reference/ChEBI CHEBI 16643
xref: Reference/KEGG Compound C00073
xref: Reference/CAS 63-68-3
xref: Reference/BioCyc MET
xref: Reference/PubChem-compound 6137
xref: Reference/ChemSpider 5907
chemical formula: C5H11NO2S
molecular weight: 149.05106
structure: ChemicalStructure/PW C000548

Compound/PW C000170 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: Pyrophosphate
stoichiometric coefficient: 1.0

Reference/CAS 52-90-4
type: UnificationXref
id: 52-90-4
db: CAS

molybdenum insertase
type: ProteinReference
xref: Reference/UniProt Q39054
organism: Arabidopsis thaliana

S-Adenosylmethionine
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: S-Adenosylmethionine

Reference/PubChem-compound 25245677
type: UnificationXref
id: 25245677
db: PubChem-compound

Purine Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway4447
pathway component: SubPathwayInteraction4447
name: SubPathway

Reference/CAS 73508-07-3
type: UnificationXref
id: 73508-07-3
db: CAS

Reference/BioCyc AMP
type: UnificationXref
id: AMP
db: BioCyc

Reference/ChEBI CHEBI 25371
type: UnificationXref
id: CHEBI:25371
db: ChEBI

Reference/KEGG Compound C00041
type: UnificationXref
id: C00041
db: KEGG Compound

Mitochondrial Matrix
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005759

ChemicalStructure/PW C040077
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C040076
type: ChemicalStructure
structure format: SMILES

Reference/HMDB HMDB0000250
type: UnificationXref
id: HMDB0000250
db: HMDB

Reference/KEGG Compound C18239
type: UnificationXref
id: C18239
db: KEGG Compound

ChemicalStructure/PW C000170
type: ChemicalStructure
structure format: SMILES
structure data: OP(O)(=O)OP(O)(O)=O

Compound/PW C000448 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Cysteine
stoichiometric coefficient: 1.0

Reference/CAS 14000-31-8
type: UnificationXref
id: 14000-31-8
db: CAS

Reference/ChEBI CHEBI 21437
type: UnificationXref
id: CHEBI:21437
db: ChEBI

Reference/KEGG Compound C00044
type: UnificationXref
id: C00044
db: KEGG Compound

SubPathwayInteraction4447
type: Interaction
name: SubPathwayReaction
name: SubPathway4447Reaction
participant: Guanosine triphosphate

Compound/PW C000548 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Methionine
stoichiometric coefficient: 1.0

ChemicalStructure/PW C007807
type: ChemicalStructure
structure format: SMILES

Reference/ChEBI CHEBI 132069
type: UnificationXref
id: CHEBI:132069
db: ChEBI

Reference/PubChem-compound 70680283
type: UnificationXref
id: 70680283
db: PubChem-compound

ChemicalStructure/PW C007805
type: ChemicalStructure
structure format: SMILES
structure data: O[Mo++](O)(=O)=O

Compound/PW C000170/Stoichiometry/1.0
type: Stoichiometry
physical entity: Pyrophosphate
stoichiometric coefficient: 1.0

Thio-molybdenum cofactor
type: SmallMolecule
cellular location: Cytosol
entity reference: Thio-molybdenum cofactor

Reference/HMDB HMDB0002206
type: UnificationXref
id: HMDB0002206
db: HMDB

reduced electron acceptor
type: SmallMolecule
cellular location: Cytosol
entity reference: reduced electron acceptor

Compound/PW C000105 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: L-Alanine
stoichiometric coefficient: 1.0

Transport888
type: Transport
participant stoichiometry: Compound/PW C040076 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040076 Mitochondrial Matrix/Stoichiometry/1.0
right: Cyclic pyranopterin monophosphate
spontaneous: false
left: Cyclic pyranopterin monophosphate
name: Cyclic pyranopterin monophosphate (→) Transport: Arabidopsis thaliana, Cell, Mitochondrial Matrix to Arabidopsis thaliana, Cell, Cytosol
conversion direction: LEFT_TO_RIGHT

Compound/PW C000032 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine monophosphate
stoichiometric coefficient: 1.0

Reference/PathWhiz PW012907
type: UnificationXref
id: PW012907
db: PathWhiz

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

molybdenum insertase
type: Protein
cellular location: Cytosol
entity reference: molybdenum insertase

Reference/BioCyc CPD-8123
type: UnificationXref
id: CPD-8123
db: BioCyc

Arabidopsis thaliana
type: BioSource
xref: Reference/TAXONOMY 3702

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Reference/KEGG Compound C21310
type: UnificationXref
id: C21310
db: KEGG Compound

Reference/UniProt Q9C5X8
type: UnificationXref
id: Q9C5X8
db: UniProt

GTP 3',8-cyclase
type: Protein
cellular location: Mitochondrial Matrix
entity reference: GTP 3',8-cyclase

Compound/PW C000921 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: S-Adenosylmethionine
stoichiometric coefficient: 1.0

Reference/KEGG Compound C05198
type: UnificationXref
id: C05198
db: KEGG Compound

Compound/Generic227 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: oxidized electron acceptor
stoichiometric coefficient: 1.0

Reference/CAS 61-19-8
type: UnificationXref
id: 61-19-8
db: CAS

ChemicalStructure/PW C000032
type: ChemicalStructure
structure format: SMILES

ChemicalStructure/PW C057876
type: ChemicalStructure
structure format: SMILES
structure data: NC1=NC2=C(NC3N2C2OC(COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)C3(O)C2O)C(=O)N1

cPMP synthase
type: Protein
cellular location: Mitochondrial Matrix
entity reference: cPMP synthase

ChemicalStructure/PW C057878
type: ChemicalStructure
structure format: SMILES
structure data: NC1=NC2=C(NC3C(N2)OC(COP([O-])([O-])=O)C2=C3S[Mo](=O)(=O)S2)C(=O)N1

Reference/CAS 63-68-3
type: UnificationXref
id: 63-68-3
db: CAS

Compound/PW C007807 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Molybdopterin-AMP
stoichiometric coefficient: 1.0

Reference/ChemSpider 5907
type: UnificationXref
id: 5907
db: ChemSpider

Water
type: SmallMolecule
cellular location: Cytosol
entity reference: Water

Reference/ChEBI CHEBI 16643
type: UnificationXref
id: CHEBI:16643
db: ChEBI

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

Reference/PubChem-compound 119058132
type: UnificationXref
id: 119058132
db: PubChem-compound

Reference/HMDB HMDB0012260
type: UnificationXref
id: HMDB0012260
db: HMDB

Reference/HMDB HMDB0012262
type: UnificationXref
id: HMDB0012262
db: HMDB

Reference/HMDB HMDB0000045
type: UnificationXref
id: HMDB0000045
db: HMDB

Reference/KEGG Compound C00073
type: UnificationXref
id: C00073
db: KEGG Compound

Reference/HMDB HMDB0000161
type: UnificationXref
id: HMDB0000161
db: HMDB

Compound/PW C001420 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

ChemicalStructure/PW C001491
type: ChemicalStructure
structure format: SMILES

Molybdopterin
type: SmallMolecule
cellular location: Cytosol
entity reference: Molybdopterin

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Reference/ChemSpider 31983
type: UnificationXref
id: 31983
db: ChemSpider

L-Alanine
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00041
xref: Reference/ChemSpider 5735
xref: Reference/ChEBI CHEBI 16977
xref: Reference/BioCyc L-ALPHA-ALANINE
xref: Reference/HMDB HMDB0000161
xref: Reference/CAS 56-41-7
xref: Reference/PubChem-compound 5950
chemical formula: C3H7NO2
molecular weight: 89.047676
structure: ChemicalStructure/PW C000105

L-Alanine
type: SmallMolecule
cellular location: Cytosol
entity reference: L-Alanine

molybdenum cofactor sulfurtransferase
type: Protein
cellular location: Cytosol
entity reference: molybdenum cofactor sulfurtransferase

reduced electron acceptor
type: SmallMolecule
cellular location: Mitochondrial Matrix
entity reference: reduced electron acceptor

Compound/PW C040034 Cytosol/Stoichiometry/4.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 4.0

3',8-cH2GTP
type: SmallMoleculeReference
xref: Reference/BioCyc CPD-19179
xref: Reference/PubChem-compound 119058132
xref: Reference/KEGG Compound C21310
xref: Reference/ChEBI CHEBI 132069
chemical formula: C10H12N5O14P3
molecular weight: 518.96155
structure: ChemicalStructure/PW C057876

Reference/KEGG Compound C00080
type: UnificationXref
id: C00080
db: KEGG Compound

molybdenum cofactor (Moco)
type: SmallMoleculeReference
xref: Reference/BioCyc CPD-8123
xref: Reference/ChEBI CHEBI 71302
xref: Reference/PubChem-compound 70680283
chemical formula: C10H10MoN5O8PS2
molecular weight: 520.8773
structure: ChemicalStructure/PW C057878

Compound/PW C001491/Stoichiometry/1.0
type: Stoichiometry
physical entity: Molybdopterin
stoichiometric coefficient: 1.0

Compound/Generic237 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: reduced electron acceptor
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

L-Cysteine
type: SmallMolecule
cellular location: Cytosol
entity reference: L-Cysteine

Reference/ChEBI CHEBI 15378
type: UnificationXref
id: CHEBI:15378
db: ChEBI

ChemicalStructure/PW C040034
type: ChemicalStructure
structure format: SMILES
structure data: [H+]

Reference/UniProt Q39056
type: UnificationXref
id: Q39056
db: UniProt

Reference/ChEBI CHEBI 17319
type: UnificationXref
id: CHEBI:17319
db: ChEBI

Reference/ChemSpider 74188
type: UnificationXref
id: 74188
db: ChemSpider

Reference/ChemSpider 6569
type: UnificationXref
id: 6569
db: ChemSpider

Compound/PW C040034 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Reference/UniProt Q39054
type: UnificationXref
id: Q39054
db: UniProt

Reference/UniProt Q39055
type: UnificationXref
id: Q39055
db: UniProt

4.1.99.22
type: BiochemicalReaction
participant stoichiometry: Compound/Generic227 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/Generic237 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001329 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000986 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C057876 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000548 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000921 Mitochondrial Matrix/Stoichiometry/1.0
right: 3',8-cH2GTP
right: oxidized electron acceptor
right: L-Methionine
right: 5'-Deoxyadenosine
right: Hydrogen Ion
spontaneous: false
EC number: 4.1.99.22
left: Guanosine triphosphate
left: reduced electron acceptor
left: S-Adenosylmethionine
name: Guanosine triphosphate + S-Adenosylmethionine + reduced electron acceptor → 3',8-cH2GTP + 5'-Deoxyadenosine + Hydrogen Ion + L-Methionine + oxidized electron acceptor
conversion direction: LEFT_TO_RIGHT

4.6.1.17
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000170 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C057876 Mitochondrial Matrix/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040076 Mitochondrial Matrix/Stoichiometry/1.0
right: Pyrophosphate
right: Cyclic pyranopterin monophosphate
spontaneous: false
EC number: 4.6.1.17
left: 3',8-cH2GTP
name: 3',8-cH2GTP → Cyclic pyranopterin monophosphate + Pyrophosphate
conversion direction: LEFT_TO_RIGHT

Reference/BioCyc GTP
type: UnificationXref
id: GTP
db: BioCyc

5'-Deoxyadenosine
type: SmallMoleculeReference
xref: Reference/KEGG Compound C05198
xref: Reference/ChemSpider 388325
xref: Reference/CAS 4754-39-6
xref: Reference/HMDB HMDB0001983
xref: Reference/ChEBI CHEBI 17319
xref: Reference/BioCyc CH33ADO
xref: Reference/PubChem-compound 439182
chemical formula: C10H13N5O3
molecular weight: 251.10184
structure: ChemicalStructure/PW C001329

2.8.1.12
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040076 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001491 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034 Cytosol/Stoichiometry/4.0
right: Hydrogen Ion
right: Molybdopterin
spontaneous: false
EC number: 2.8.1.12
left: Cyclic pyranopterin monophosphate
left: Water
name: Cyclic pyranopterin monophosphate + Water → 4 Hydrogen Ion + Molybdopterin
conversion direction: LEFT_TO_RIGHT

Reference/UniProt Q9LVM1
type: UnificationXref
id: Q9LVM1
db: UniProt

Compound/PW C040076 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: Cyclic pyranopterin monophosphate
stoichiometric coefficient: 1.0

2.7.7.75
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000414/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001491/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000170/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007807/Stoichiometry/1.0
participant stoichiometry: Compound/PW C040034/Stoichiometry/1.0
right: Molybdopterin-AMP
right: Pyrophosphate
spontaneous: false
EC number: 2.7.7.75
left: Molybdopterin
left: Hydrogen Ion
left: Adenosine triphosphate
name: Adenosine triphosphate + Hydrogen Ion + Molybdopterin → Molybdopterin-AMP + Pyrophosphate
conversion direction: LEFT_TO_RIGHT

Reaction47438
type: BiochemicalReaction
participant stoichiometry: Compound/PW C040034 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C057878 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007807 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000032 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C007805 Cytosol/Stoichiometry/1.0
right: Adenosine monophosphate
right: molybdenum cofactor (Moco)
right: Water
spontaneous: false
left: Molybdate
left: Hydrogen Ion
left: Molybdopterin-AMP
name: Hydrogen Ion + Molybdate + Molybdopterin-AMP → Adenosine monophosphate + Water + molybdenum cofactor (Moco)
conversion direction: LEFT_TO_RIGHT

molybdopterin synthase small subunit
type: Protein
cellular location: Cytosol
entity reference: molybdopterin synthase small subunit

Reference/ChEBI CHEBI 60210
type: UnificationXref
id: CHEBI:60210
db: ChEBI

Reference/HMDB HMDB0001273
type: UnificationXref
id: HMDB0001273
db: HMDB

Reference/PubChem-compound 644102
type: UnificationXref
id: 644102
db: PubChem-compound

L-Cysteine
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000574
xref: Reference/CAS 52-90-4
xref: Reference/KEGG Compound C00097
xref: Reference/BioCyc CYS
xref: Reference/ChemSpider 5653
xref: Reference/PubChem-compound 5862
xref: Reference/ChEBI CHEBI 17561
chemical formula: C3H7NO2S
molecular weight: 121.01975
structure: ChemicalStructure/PW C000448

reduced electron acceptor
type: SmallMoleculeReference

Reference/PubChem-compound 6083
type: UnificationXref
id: 6083
db: PubChem-compound

ChemicalStructure/PW C000986
type: ChemicalStructure
structure format: SMILES

Reference/KEGG Compound C00097
type: UnificationXref
id: C00097
db: KEGG Compound

oxidized electron acceptor
type: SmallMolecule
cellular location: Cytosol
entity reference: oxidized electron acceptor

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Compound/PW C057878 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: molybdenum cofactor (Moco)
stoichiometric coefficient: 1.0

Reference/CAS 86-01-1
type: UnificationXref
id: 86-01-1
db: CAS

cPMP synthase
type: ProteinReference
xref: Reference/UniProt Q39056
organism: Arabidopsis thaliana

GTP 3',8-cyclase
type: ProteinReference
xref: Reference/UniProt Q39055
organism: Arabidopsis thaliana

molybdopterin synthase small subunit
type: ProteinReference
xref: Reference/UniProt Q9S7A3
organism: Arabidopsis thaliana

molybdopterin synthase large subunit
type: ProteinReference
xref: Reference/UniProt O22827
organism: Arabidopsis thaliana

Reference/HMDB HMDB0059597
type: UnificationXref
id: HMDB0059597
db: HMDB

oxidized electron acceptor
type: SmallMoleculeReference

Compound/PW C040076 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Cyclic pyranopterin monophosphate
stoichiometric coefficient: 1.0

Reference/PubChem-compound 16061579
type: UnificationXref
id: 16061579
db: PubChem-compound

Reference/PubChem-compound 24762165
type: UnificationXref
id: 24762165
db: PubChem-compound

Compound/Generic237 Mitochondrial Matrix/Stoichiometry/1.0
type: Stoichiometry
physical entity: reduced electron acceptor
stoichiometric coefficient: 1.0

TransportCatalysis788
type: Catalysis
controller: ABC transporter of the mitochondrion 3
controlled: Transport888
control type: ACTIVATION

Compound/PW C040077 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Thio-molybdenum cofactor
stoichiometric coefficient: 1.0

ABC transporter of the mitochondrion 3
type: ProteinReference
xref: Reference/UniProt Q9LVM1

Reference/PubChem-compound 82208
type: UnificationXref
id: 82208
db: PubChem-compound