PIK3R6 protein
type: Protein
cellular location: Cytosol
entity reference: PIK3R6 protein

SHC-transforming protein 1
type: Protein
cellular location: Cytosol
entity reference: SHC-transforming protein 1

Reference/TAXONOMY 9913
type: UnificationXref
id: 9913
db: TAXONOMY

RAF proto-oncogene serine/threonine-protein kinase
type: Protein
cellular location: Cell Membrane
entity reference: RAF proto-oncogene serine/threonine-protein kinase

Protein/19628 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: GRB2-related adapter protein
stoichiometric coefficient: 1.0

PIP3(16:0/16:0)
type: SmallMolecule
cellular location: Cytoplasm
entity reference: PIP3(16:0/16:0)

GRB2-SOS-IRS-SHC complex
type: Complex
component stoichiometry: Protein/19628 Cell Membrane/Stoichiometry/1.0
component stoichiometry: Protein/19396 Cell Membrane/Stoichiometry/1.0
component stoichiometry: Protein/19663 Cell Membrane/Stoichiometry/1.0
component stoichiometry: Protein/19659 Cell Membrane/Stoichiometry/1.0
cellular location: Cell Membrane
component: SHC-transforming protein 1
component: Rap guanine nucleotide exchange factor 2
component: Insulin receptor substrate 1
component: GRB2-related adapter protein

Insulin
type: Complex
component stoichiometry: Protein/19669 Extracellular Space/Stoichiometry/2.0
cellular location: Extracellular Space
component: Insulin
name: SubPathwayActivator

Protein/19407 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Dual specificity mitogen-activated protein kinase kinase 6
stoichiometric coefficient: 1.0

Protein/19396 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Rap guanine nucleotide exchange factor 2
stoichiometric coefficient: 1.0

Protein/19661 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Insulin receptor substrate 2
stoichiometric coefficient: 1.0

Mitogen-activated protein kinase 1
type: Protein
cellular location: Cytosol
entity reference: Mitogen-activated protein kinase 1

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Rap guanine nucleotide exchange factor 2
type: ProteinReference
xref: Reference/UniProt F1MSG6
organism: Bos taurus

Reference/CAS 2280-44-6
type: UnificationXref
id: 2280-44-6
db: CAS

Protein/19500 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: RAC-alpha serine/threonine-protein kinase
stoichiometric coefficient: 1.0

Insulin receptor substrate 1
type: Protein
cellular location: Cell Membrane
entity reference: Insulin receptor substrate 1

Reference/ChemSpider 24767909
type: UnificationXref
id: 24767909
db: ChemSpider

Reference/UniProt A6QLK6
type: UnificationXref
id: A6QLK6
db: UniProt

Starch and Sucrose Metabolism
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway109220
pathway component: SubPathwayInteraction109220
xref: Reference/SMPDB SMP0000058
xref: Reference/PathWhiz PW000150
name: SubPathway

Reference/UniProt P32871
type: UnificationXref
id: P32871
db: UniProt

Ras-related protein Rap-1b
type: Protein
cellular location: Cytosol
entity reference: Ras-related protein Rap-1b

Protein/19409 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: RAF proto-oncogene serine/threonine-protein kinase
stoichiometric coefficient: 1.0

Reference/PubChem-compound 6830
type: UnificationXref
id: 6830
db: PubChem-compound

TransportCatalysis1239
type: Catalysis
controller: Solute carrier family 2, facilitated glucose transporter member 4
controlled: Transport10005
control type: ACTIVATION

Reference/PathWhiz PW000150
type: UnificationXref
id: PW000150
db: PathWhiz

Guanosine diphosphate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001201
xref: Reference/BioCyc GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE
xref: Reference/CAS 146-91-8
xref: Reference/PubChem-compound 8977
xref: Reference/ChEBI CHEBI 17552
xref: Reference/ChemSpider 8630
xref: Reference/KEGG Compound C00035
chemical formula: C10H15N5O11P2
molecular weight: 443.02432
structure: ChemicalStructure/PW C000936

Compound/PW C006073 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: PIP2(16:0/16:0)
stoichiometric coefficient: 1.0

Protein/19430 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ras-related protein Rap-1b
stoichiometric coefficient: 1.0

2.7.11.1
type: BiochemicalReaction
participant stoichiometry: Protein/19407 Cell Membrane/Stoichiometry/1.0
right: Dual specificity mitogen-activated protein kinase kinase 6
spontaneous: false
EC number: 2.7.11.1
left: Dual specificity mitogen-activated protein kinase kinase 6
name: Inactive Dual specificity mitogen-activated protein kinase kinase 1 → Active Dual specificity mitogen-activated protein kinase kinase 1
conversion direction: LEFT_TO_RIGHT

2.7.11.1
type: BiochemicalReaction
participant stoichiometry: Protein/19407 Cell Membrane/Stoichiometry/1.0
right: Dual specificity mitogen-activated protein kinase kinase 6
spontaneous: false
EC number: 2.7.11.1
left: Dual specificity mitogen-activated protein kinase kinase 6
name: Inactive Dual specificity mitogen-activated protein kinase kinase 2 → Active Dual specificity mitogen-activated protein kinase kinase 2
conversion direction: LEFT_TO_RIGHT

2.7.12.2
type: BiochemicalReaction
participant stoichiometry: Protein/19413 Cytosol/Stoichiometry/1.0
right: Mitogen-activated protein kinase 1
spontaneous: false
EC number: 2.7.12.2
left: Mitogen-activated protein kinase 1
name: Inactive Mitogen-activated protein kinase 1 → Active Mitogen-activated protein kinase 1
conversion direction: LEFT_TO_RIGHT

2.7.12.2
type: BiochemicalReaction
participant stoichiometry: Protein/19413 Cytosol/Stoichiometry/1.0
right: Mitogen-activated protein kinase 1
spontaneous: false
EC number: 2.7.12.2
left: Mitogen-activated protein kinase 1
name: Inactive Mitogen-activated protein kinase 1 → Active Mitogen-activated protein kinase 1
conversion direction: LEFT_TO_RIGHT

Guanosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15996
xref: Reference/ChemSpider 6569
xref: Reference/KEGG Compound C00044
xref: Reference/PubChem-compound 6830
xref: Reference/HMDB HMDB0001273
xref: Reference/CAS 86-01-1
xref: Reference/BioCyc GTP
chemical formula: C10H16N5O14P3
molecular weight: 522.99066
structure: ChemicalStructure/PW C000986

Insulin-like growth factor 1 receptor
type: Protein
cellular location: Cell Membrane
entity reference: Insulin-like growth factor 1 receptor
name: SubPathwayActivation

Guanosine triphosphate
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Guanosine triphosphate

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

2.7.11.1
type: BiochemicalReaction
participant stoichiometry: Protein/19500 Cytosol/Stoichiometry/1.0
right: RAC-alpha serine/threonine-protein kinase
spontaneous: false
EC number: 2.7.11.1
left: RAC-alpha serine/threonine-protein kinase
name: RAC-alpha serine/threonine-protein kinase → RAC-alpha serine/threonine-protein kinase with phosphorylation
conversion direction: LEFT_TO_RIGHT

Reaction174251
type: BiochemicalReaction
participant stoichiometry: Protein/19430 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11259 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Protein/19409 Cell Membrane/Stoichiometry/1.0
right: RAF proto-oncogene serine/threonine-protein kinase recruited by GTPase HRas
spontaneous: true
left: RAF proto-oncogene serine/threonine-protein kinase
left: Ras-related protein Rap-1b
name: GTPase HRas with GTP + RAF proto-oncogene serine/threonine-protein kinase → RAF proto-oncogene serine/threonine-protein kinase recruited by GTPase HRas
conversion direction: LEFT_TO_RIGHT

Transport10005
type: Transport
participant stoichiometry: Compound/PW C000077 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000077 Extracellular Space/Stoichiometry/1.0
right: D-Glucose
spontaneous: false
left: D-Glucose
name: D-Glucose (→) Transport: Bos taurus, Extracellular Space to Bos taurus, Cell, Cytosol
conversion direction: LEFT_TO_RIGHT

Phosphatidylinositol 4,5-bisphosphate 3-kinase
type: Complex
component stoichiometry: Protein/16922 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19654 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: PIK3R6 protein
component: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Ras-related protein Rap-1b
type: Protein
cellular location: Cell Membrane
entity reference: Ras-related protein Rap-1b

Reference/GENE ONTOLOGY GO 0005886
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

D-Glucose
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000122
xref: Reference/PubChem-compound 5793
xref: Reference/CAS 2280-44-6
xref: Reference/ChemSpider 5589
xref: Reference/ChEBI CHEBI 4167
xref: Reference/BioCyc GLC
xref: Reference/KEGG Compound C00031
chemical formula: C6H12O6
molecular weight: 180.06339
structure: ChemicalStructure/PW C000077

Reaction174243
type: BiochemicalReaction
participant stoichiometry: Compound/PW C006073 Cytoplasm/Stoichiometry/1.0
participant stoichiometry: Compound/PW C006188 Cytoplasm/Stoichiometry/1.0
right: PIP3(16:0/16:0)
spontaneous: false
left: PIP2(16:0/16:0)
name: PIP2(16:0/16:0) → PIP3(16:0/16:0)
conversion direction: LEFT_TO_RIGHT

Reference/BioCyc Phosphatidylinositols
type: UnificationXref
id: Phosphatidylinositols
db: BioCyc

Reaction174244
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/10501 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11254 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/19430 Cytosol/Stoichiometry/1.0
right: Activated Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
spontaneous: true
left: Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
left: Ras-related protein Rap-1b
name: GTPase HRas with GTP + Phosphatidylinositol 4,5-bisphosphate 3-kinase complex → Activated Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
conversion direction: LEFT_TO_RIGHT

Compound/PW C000077 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: D-Glucose
stoichiometric coefficient: 1.0

Reference/UniProt F1MYE0
type: UnificationXref
id: F1MYE0
db: UniProt

Reference/PubChem-compound 53480223
type: UnificationXref
id: 53480223
db: PubChem-compound

ReactionCatalysis164465
type: Catalysis
controller: Dual specificity mitogen-activated protein kinase kinase 6
controlled: 2.7.12.2
control type: ACTIVATION

2.7.10.1
type: BiochemicalReaction
participant stoichiometry: Protein/19663 Cytosol/Stoichiometry/1.0
right: SHC-transforming protein 1
spontaneous: false
EC number: 2.7.10.1
left: SHC-transforming protein 1
name: SHC-transforming protein 1 → SHC-transforming protein 1 with phosphorylation
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis164464
type: Catalysis
controller: RAF proto-oncogene serine/threonine-protein kinase recruited by GTPase HRas
controlled: 2.7.11.1
control type: ACTIVATION

Reaction174247
type: BiochemicalReaction
participant stoichiometry: Protein/19663 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/19628 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11257 Cytosol/Stoichiometry/1.0
right: GRB2-IRS-SHC complex
spontaneous: true
left: Insulin receptor substrate 1
left: GRB2-related adapter protein
left: SHC-transforming protein 1
name: Growth factor receptor-bound protein 2 + Insulin receptor substrate 1 with Phosphorylation + SHC-transforming protein 1 with phosphorylation → GRB2-IRS-SHC complex
conversion direction: LEFT_TO_RIGHT

Reaction174248
type: BiochemicalReaction
participant stoichiometry: Protein/19396 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11257 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/11258 Cytosol/Stoichiometry/1.0
right: GRB2-SOS-IRS-SHC complex
spontaneous: true
left: GRB2-IRS-SHC complex
left: Rap guanine nucleotide exchange factor 2
name: GRB2-IRS-SHC complex + Son of sevenless homolog 1 → GRB2-SOS-IRS-SHC complex
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis164466
type: Catalysis
controller: Dual specificity mitogen-activated protein kinase kinase 6
controlled: 2.7.12.2
control type: ACTIVATION

ReactionCatalysis164461
type: Catalysis
controller: GRB2-SOS-IRS-SHC complex
controlled: Reaction174249
control type: ACTIVATION

Reference/BioCyc GLC
type: UnificationXref
id: GLC
db: BioCyc

ProteinComplex/10515 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol 4,5-bisphosphate 3-kinase
stoichiometric coefficient: 1.0

ReactionCatalysis164460
type: Catalysis
controller: Insulin-like growth factor 1 receptor
controlled: 2.7.10.1
control type: ACTIVATION

ReactionCatalysis164463
type: Catalysis
controller: RAF proto-oncogene serine/threonine-protein kinase recruited by GTPase HRas
controlled: 2.7.11.1
control type: ACTIVATION

Reference/HMDB HMDB0010147
type: UnificationXref
id: HMDB0010147
db: HMDB

ReactionCatalysis164462
type: Catalysis
controller: cAMP-dependent protein kinase catalytic subunit beta
controlled: 2.7.11.1
control type: ACTIVATION

ChemicalStructure/PW C000936
type: ChemicalStructure
structure format: SMILES

Insulin Signalling
type: Pathway
pathway order: PathwayEnzymeReaction/2118049
pathway order: PathwayEnzymeReaction/2118048
pathway order: PathwaySubPathway/109220
pathway order: PathwaySubPathway/109219
pathway order: PathwaySubPathway/109218
pathway order: PathwayEnzymeReaction/2118050
pathway order: PathwayTransport/2118056
pathway order: PathwayEnzymeReaction/2118045
pathway order: PathwayEnzymeReaction/2118044
pathway order: PathwayEnzymeReaction/2118055
pathway order: PathwayEnzymeReaction/2118047
pathway order: PathwayEnzymeReaction/2118046
pathway order: PathwayEnzymeReaction/2118041
pathway order: PathwayEnzymeReaction/2118052
pathway order: PathwayEnzymeReaction/2118051
pathway order: PathwayEnzymeReaction/2118043
pathway order: PathwayEnzymeReaction/2118054
pathway order: PathwayEnzymeReaction/2118042
pathway order: PathwayEnzymeReaction/2118053
pathway component: ReactionCatalysis164458
pathway component: 2.7.11.1
pathway component: 2.7.12.2
pathway component: Reaction174251
pathway component: Transport10005
pathway component: SubPathwayControl109218
pathway component: SubPathwayControl109219
pathway component: Starch and Sucrose Metabolism
pathway component: Reaction174243
pathway component: Reaction174244
pathway component: ReactionCatalysis164465
pathway component: 2.7.10.1
pathway component: ReactionCatalysis164464
pathway component: Reaction174247
pathway component: Reaction174248
pathway component: ReactionCatalysis164466
pathway component: ReactionCatalysis164461
pathway component: ReactionCatalysis164460
pathway component: ReactionCatalysis164463
pathway component: ReactionCatalysis164462
pathway component: TransportCatalysis1239
pathway component: ReactionCatalysis164184
pathway component: ReactionCatalysis164183
pathway component: Reaction174249
pathway component: Reaction173898
xref: Reference/SMPDB SMP0108189
xref: Reference/PathWhiz PW109231
organism: Bos taurus

Protein/16922 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
stoichiometric coefficient: 1.0

RAC-alpha serine/threonine-protein kinase
type: Protein
cellular location: Cytoplasm
entity reference: RAC-alpha serine/threonine-protein kinase
name: SubPathwayActivator

Reference/BioCyc GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE
type: UnificationXref
id: GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE
db: BioCyc

Protein/19659 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Insulin receptor substrate 1
stoichiometric coefficient: 1.0

ProteinComplex/11254 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Activated Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
stoichiometric coefficient: 1.0

Reaction174249
type: BiochemicalReaction
participant stoichiometry: Protein/19430 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000936 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000986 Cell Membrane/Stoichiometry/1.0
right: Guanosine diphosphate
right: Ras-related protein Rap-1b
spontaneous: false
left: Guanosine triphosphate
left: Ras-related protein Rap-1b
name: GTPase HRas with GDP + Guanosine triphosphate → GTPase HRas with GTP + Guanosine diphosphate
conversion direction: LEFT_TO_RIGHT

Reference/SMPDB SMP0000058
type: UnificationXref
id: SMP0000058
db: SMPDB

PIP3(16:0/16:0)
type: SmallMoleculeReference
xref: Reference/BioCyc Phosphatidylinositols
xref: Reference/KEGG Compound C00626
xref: Reference/HMDB HMDB0010147
xref: Reference/ChemSpider 24768024
chemical formula: C41H82O22P4
molecular weight: 1050.4248
structure: ChemicalStructure/PW C006188

ReactionCatalysis164458
type: Catalysis
controller: Activated Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
controlled: Reaction174243
control type: ACTIVATION

Reference/HMDB HMDB0010032
type: UnificationXref
id: HMDB0010032
db: HMDB

Protein/19659 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Insulin receptor substrate 1
stoichiometric coefficient: 1.0

Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
type: Complex
component stoichiometry: Protein/19661 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/16922 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19654 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Insulin receptor substrate 1
component: PIK3R6 protein
component: Insulin receptor substrate 2
component: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Reference/UniProt A7YY51
type: UnificationXref
id: A7YY51
db: UniProt

Reference/UniProt Q3T0M4
type: UnificationXref
id: Q3T0M4
db: UniProt

RAF proto-oncogene serine/threonine-protein kinase recruited by GTPase HRas
type: Complex
component stoichiometry: Protein/19430 Cell Membrane/Stoichiometry/1.0
component stoichiometry: Protein/19409 Cell Membrane/Stoichiometry/1.0
cellular location: Cell Membrane
component: RAF proto-oncogene serine/threonine-protein kinase
component: Ras-related protein Rap-1b

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Insulin receptor substrate 1
type: Protein
cellular location: Cytosol
entity reference: Insulin receptor substrate 1

Reference/HMDB HMDB0001201
type: UnificationXref
id: HMDB0001201
db: HMDB

SHC-transforming protein 1
type: Protein
cellular location: Cell Membrane
entity reference: SHC-transforming protein 1

cAMP-dependent protein kinase catalytic subunit beta
type: ProteinReference
xref: Reference/UniProt P24256
organism: Bos taurus

SubPathwayControl109218
type: Interaction
name: SubPathway109218Control
name: ActivatingSubPathwayControl
participant: Insulin-like growth factor 1 receptor
participant: Insulin receptor activation by insulin
participant: Insulin

SubPathwayControl109219
type: Interaction
name: SubPathway109219Control
name: ActivatingSubPathwayControl
participant: RAC-alpha serine/threonine-protein kinase
participant: GLUT4 activation by AKT
participant: Solute carrier family 2, facilitated glucose transporter member 4

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit beta

Ras-related protein Rap-1b
type: Protein
cellular location: Cytoplasm
entity reference: Ras-related protein Rap-1b

Reference/ChEBI CHEBI 15996
type: UnificationXref
id: CHEBI:15996
db: ChEBI

Reference/UniProt Q05688
type: UnificationXref
id: Q05688
db: UniProt

ProteinComplex/11257 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: GRB2-IRS-SHC complex
stoichiometric coefficient: 1.0

GRB2-SOS-IRS-SHC complex
type: Complex
component stoichiometry: Protein/19663 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19628 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19396 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Insulin receptor substrate 1
component: Rap guanine nucleotide exchange factor 2
component: GRB2-related adapter protein
component: SHC-transforming protein 1

Reference/HMDB HMDB0000122
type: UnificationXref
id: HMDB0000122
db: HMDB

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

ProteinComplex/11258 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: GRB2-SOS-IRS-SHC complex
stoichiometric coefficient: 1.0

Reference/UniProt P01317
type: UnificationXref
id: P01317
db: UniProt

Guanosine diphosphate
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Guanosine diphosphate

Protein/19663 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: SHC-transforming protein 1
stoichiometric coefficient: 1.0

PIP2(16:0/16:0)
type: SmallMolecule
cellular location: Cytoplasm
entity reference: PIP2(16:0/16:0)

RAC-alpha serine/threonine-protein kinase
type: Protein
cellular location: Cytosol
entity reference: RAC-alpha serine/threonine-protein kinase

Solute carrier family 2, facilitated glucose transporter member 4
type: Protein
cellular location: Cell Membrane
entity reference: Solute carrier family 2, facilitated glucose transporter member 4
name: SubPathwayActivation

Reference/KEGG Compound C00031
type: UnificationXref
id: C00031
db: KEGG Compound

Protein/19396 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Rap guanine nucleotide exchange factor 2
stoichiometric coefficient: 1.0

GRB2-related adapter protein
type: Protein
cellular location: Cell Membrane
entity reference: GRB2-related adapter protein

Reference/KEGG Compound C00035
type: UnificationXref
id: C00035
db: KEGG Compound

Reference/UniProt Q45KX9
type: UnificationXref
id: Q45KX9
db: UniProt

Protein/19659 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Insulin receptor substrate 1
stoichiometric coefficient: 1.0

Reference/UniProt A2VE60
type: UnificationXref
id: A2VE60
db: UniProt

D-Glucose
type: SmallMolecule
cellular location: Cytosol
entity reference: D-Glucose

Mitogen-activated protein kinase 1
type: ProteinReference
xref: Reference/UniProt A2VE60
organism: Bos taurus

PIK3R6 protein
type: ProteinReference
xref: Reference/UniProt F1N418
organism: Bos taurus

Insulin receptor substrate 1
type: ProteinReference
xref: Reference/UniProt F1MYE0
organism: Bos taurus

D-Glucose
type: SmallMolecule
cellular location: Extracellular Space
entity reference: D-Glucose

ProteinComplex/10501 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00044
type: UnificationXref
id: C00044
db: KEGG Compound

Reference/ChemSpider 24768024
type: UnificationXref
id: 24768024
db: ChemSpider

Insulin-like growth factor 1 receptor
type: Protein
cellular location: Cytosol
entity reference: Insulin-like growth factor 1 receptor

Solute carrier family 2, facilitated glucose transporter member 4
type: Protein
entity reference: Solute carrier family 2, facilitated glucose transporter member 4

Insulin
type: Protein
cellular location: Extracellular Space
entity reference: Insulin

Protein/19661 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Insulin receptor substrate 2
stoichiometric coefficient: 1.0

Bos taurus
type: BioSource
xref: Reference/TAXONOMY 9913

Insulin receptor substrate 2
type: ProteinReference
xref: Reference/UniProt F1MJE0
organism: Bos taurus

SHC-transforming protein 1
type: ProteinReference
xref: Reference/UniProt Q45KX9
organism: Bos taurus

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

Insulin
type: ProteinReference
xref: Reference/UniProt P01317
organism: Bos taurus

Compound/PW C000986 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Guanosine triphosphate
stoichiometric coefficient: 1.0

Reference/ChemSpider 5589
type: UnificationXref
id: 5589
db: ChemSpider

Reference/UniProt Q6SI69
type: UnificationXref
id: Q6SI69
db: UniProt

Dual specificity mitogen-activated protein kinase kinase 6
type: Protein
cellular location: Cell Membrane
entity reference: Dual specificity mitogen-activated protein kinase kinase 6

Insulin-like growth factor 1 receptor
type: ProteinReference
xref: Reference/UniProt Q05688
organism: Bos taurus

Ras-related protein Rap-1b
type: ProteinReference
xref: Reference/UniProt Q3T0M4
organism: Bos taurus

PIP2(16:0/16:0)
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0010032
xref: Reference/BioCyc Phosphatidylinositols
xref: Reference/PubChem-compound 53480223
xref: Reference/KEGG Compound C00626
xref: Reference/ChemSpider 24767909
chemical formula: C41H81O19P3
molecular weight: 970.4585
structure: ChemicalStructure/PW C006073

Protein/19669 Extracellular Space/Stoichiometry/2.0
type: Stoichiometry
physical entity: Insulin
stoichiometric coefficient: 2.0

Protein/19663 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: SHC-transforming protein 1
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00626
type: UnificationXref
id: C00626
db: KEGG Compound

2.7.10.1
type: BiochemicalReaction
participant stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
right: Insulin receptor substrate 1
spontaneous: false
EC number: 2.7.10.1
left: Insulin receptor substrate 1
name: Insulin receptor substrate 1 → Insulin receptor substrate 1 with Phosphorylation
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 8977
type: UnificationXref
id: 8977
db: PubChem-compound

Compound/PW C000077 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: D-Glucose
stoichiometric coefficient: 1.0

Reference/UniProt F1MJE0
type: UnificationXref
id: F1MJE0
db: UniProt

2.7.10.1
type: BiochemicalReaction
participant stoichiometry: Protein/19661 Cytosol/Stoichiometry/1.0
right: Insulin receptor substrate 2
spontaneous: false
EC number: 2.7.10.1
left: Insulin receptor substrate 2
name: Insulin receptor substrate 2 → Insulin receptor substrate 2 with Phosphorylation
conversion direction: LEFT_TO_RIGHT

Reaction173898
type: BiochemicalReaction
participant stoichiometry: Protein/19661 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/10501 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/10515 Cytosol/Stoichiometry/1.0
right: Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
spontaneous: true
left: Insulin receptor substrate 1
left: Insulin receptor substrate 2
left: Phosphatidylinositol 4,5-bisphosphate 3-kinase
name: Insulin receptor substrate 1 with Phosphorylation + Insulin receptor substrate 2 with Phosphorylation + Phosphatidylinositol 4,5-bisphosphate 3-kinase → Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
conversion direction: LEFT_TO_RIGHT

Reference/UniProt F1MSG6
type: UnificationXref
id: F1MSG6
db: UniProt

SubPathwayInteraction109220
type: Interaction
name: SubPathwayReaction
name: SubPathway109220Reaction
participant: D-Glucose

Insulin receptor substrate 2
type: Protein
cellular location: Cytoplasm
entity reference: Insulin receptor substrate 2

Protein/19413 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Mitogen-activated protein kinase 1
stoichiometric coefficient: 1.0

Reference/ChemSpider 8630
type: UnificationXref
id: 8630
db: ChemSpider

GRB2-related adapter protein
type: Protein
cellular location: Cytosol
entity reference: GRB2-related adapter protein

Protein/19654 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: PIK3R6 protein
stoichiometric coefficient: 1.0

ProteinComplex/11259 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: RAF proto-oncogene serine/threonine-protein kinase recruited by GTPase HRas
stoichiometric coefficient: 1.0

ChemicalStructure/PW C006073
type: ChemicalStructure
structure format: SMILES

Cell Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005886

Insulin receptor substrate 2
type: Protein
cellular location: Cytosol
entity reference: Insulin receptor substrate 2

PIK3R6 protein
type: Protein
cellular location: Cytoplasm
entity reference: PIK3R6 protein

GLUT4 activation by AKT
type: Pathway
name: ActivatingSubPathway

Reference/ChemSpider 6569
type: UnificationXref
id: 6569
db: ChemSpider

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
type: Protein
cellular location: Cytoplasm
entity reference: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Insulin receptor activation by insulin
type: Pathway
name: ActivatingSubPathway

Reference/SMPDB SMP0108189
type: UnificationXref
id: SMP0108189
db: SMPDB

Reference/ChEBI CHEBI 17552
type: UnificationXref
id: CHEBI:17552
db: ChEBI

Reference/BioCyc GTP
type: UnificationXref
id: GTP
db: BioCyc

ReactionCatalysis164184
type: Catalysis
controller: Insulin-like growth factor 1 receptor
controlled: 2.7.10.1
control type: ACTIVATION

ReactionCatalysis164183
type: Catalysis
controller: Insulin-like growth factor 1 receptor
controlled: 2.7.10.1
control type: ACTIVATION

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
type: Protein
cellular location: Cytosol
entity reference: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

GRB2-IRS-SHC complex
type: Complex
component stoichiometry: Protein/19663 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19628 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Insulin receptor substrate 1
component: GRB2-related adapter protein
component: SHC-transforming protein 1

Reference/ChEBI CHEBI 4167
type: UnificationXref
id: CHEBI:4167
db: ChEBI

Dual specificity mitogen-activated protein kinase kinase 6
type: Protein
cellular location: Cytosol
entity reference: Dual specificity mitogen-activated protein kinase kinase 6

Solute carrier family 2, facilitated glucose transporter member 4
type: ProteinReference
xref: Reference/UniProt Q6SI69

RAC-alpha serine/threonine-protein kinase
type: ProteinReference
xref: Reference/UniProt Q5ER96
organism: Bos taurus

Reference/PathWhiz PW109231
type: UnificationXref
id: PW109231
db: PathWhiz

Protein/19430 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ras-related protein Rap-1b
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0001273
type: UnificationXref
id: HMDB0001273
db: HMDB

GRB2-related adapter protein
type: ProteinReference
xref: Reference/UniProt A6QLK6
organism: Bos taurus

Reference/PubChem-compound 5793
type: UnificationXref
id: 5793
db: PubChem-compound

Activated Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
type: Complex
component stoichiometry: Protein/19661 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19659 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/16922 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19430 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/19654 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Insulin receptor substrate 1
component: PIK3R6 protein
component: Insulin receptor substrate 2
component: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
component: Ras-related protein Rap-1b

Rap guanine nucleotide exchange factor 2
type: Protein
cellular location: Cytosol
entity reference: Rap guanine nucleotide exchange factor 2

Activated Phosphatidylinositol 4,5-bisphosphate 3-kinase complex
type: Complex
component stoichiometry: Protein/16922 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/19654 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/19661 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/19659 Cytoplasm/Stoichiometry/1.0
component stoichiometry: Protein/19430 Cytoplasm/Stoichiometry/1.0
cellular location: Cytoplasm
component: Insulin receptor substrate 1
component: Insulin receptor substrate 2
component: PIK3R6 protein
component: Ras-related protein Rap-1b
component: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Protein/16922 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
stoichiometric coefficient: 1.0

Protein/19430 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Ras-related protein Rap-1b
stoichiometric coefficient: 1.0

ChemicalStructure/PW C000986
type: ChemicalStructure
structure format: SMILES

Rap guanine nucleotide exchange factor 2
type: Protein
cellular location: Cell Membrane
entity reference: Rap guanine nucleotide exchange factor 2

Reference/UniProt Q5E9X2
type: UnificationXref
id: Q5E9X2
db: UniProt

ChemicalStructure/PW C000077
type: ChemicalStructure
structure format: SMILES
structure data: OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O

Reference/UniProt F1N418
type: UnificationXref
id: F1N418
db: UniProt

Reference/UniProt Q5ER96
type: UnificationXref
id: Q5ER96
db: UniProt

Reference/CAS 86-01-1
type: UnificationXref
id: 86-01-1
db: CAS

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
type: ProteinReference
xref: Reference/UniProt P32871
organism: Bos taurus

RAF proto-oncogene serine/threonine-protein kinase
type: ProteinReference
xref: Reference/UniProt A7YY51
organism: Bos taurus

Protein/19628 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: GRB2-related adapter protein
stoichiometric coefficient: 1.0

Reference/UniProt P24256
type: UnificationXref
id: P24256
db: UniProt

D-Glucose
type: SmallMolecule
cellular location: Cytoplasm
entity reference: D-Glucose
name: SubPathwayInput

Insulin receptor substrate 1
type: Protein
cellular location: Cytoplasm
entity reference: Insulin receptor substrate 1

Protein/19654 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: PIK3R6 protein
stoichiometric coefficient: 1.0

Compound/PW C006188 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: PIP3(16:0/16:0)
stoichiometric coefficient: 1.0

Compound/PW C000936 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Guanosine diphosphate
stoichiometric coefficient: 1.0

Reference/CAS 146-91-8
type: UnificationXref
id: 146-91-8
db: CAS

ChemicalStructure/PW C006188
type: ChemicalStructure
structure format: SMILES

Dual specificity mitogen-activated protein kinase kinase 6
type: ProteinReference
xref: Reference/UniProt Q5E9X2
organism: Bos taurus