Sodium/potassium-transporting ATPase subunit alpha-3
type: ProteinReference
xref: Reference/UniProt P13637
organism: BiologicalState794

Interaction4393
type: MolecularInteraction
interaction type: direct_interaction
name: Spironolactone + Mineralocorticoid receptor (Homo sapiens) inhibits Active Mineralocorticoid receptor (Homo sapiens) + Aldosterone
participant: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
participant: Spironolactone_Mineralocorticoid receptor (Homo sapiens)

Reference/PubChem-compound 923
type: UnificationXref
id: 923
db: PubChem-compound

Aldosterone
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000037
xref: Reference/PubChem-compound 24758425
xref: Reference/ChemSpider 24774738
xref: Reference/KEGG Compound C01780
xref: Reference/BioCyc ALDOSTERONE
xref: Reference/ChEBI CHEBI 27584
xref: Reference/CAS 52-39-1
chemical formula: C21H28O5
molecular weight: 360.19366
structure: ChemicalStructure/PW C000026

Reference/ChEBI CHEBI 9241
type: UnificationXref
id: CHEBI:9241
db: ChEBI

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

Transport10965
type: Transport
participant stoichiometry: ProteinComplex/583 Cytosol/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/583 Nucleus/Stoichiometry/1.0
right: Epithelial Sodium Channel
spontaneous: false
left: Epithelial Sodium Channel
name: Epithelial Sodium Channel (→) Transport: Homo sapiens, Kidney, Nucleus to Homo sapiens, Kidney, Cytosol
conversion direction: LEFT_TO_RIGHT

Transport10966
type: Transport
participant stoichiometry: ProteinComplex/545 Nucleus/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/545 Cytosol/Stoichiometry/1.0
right: Sodium/potassium ATPase
spontaneous: false
left: Sodium/potassium ATPase
name: Sodium/potassium ATPase (→) Transport: Homo sapiens, Kidney, Nucleus to Homo sapiens, Kidney, Cytosol
conversion direction: LEFT_TO_RIGHT

Active Mineralocorticoid receptor
type: Complex
component stoichiometry: Protein/2032/Stoichiometry/2.0
component: Mineralocorticoid receptor

direct_interaction
type: InteractionVocabulary

TransportCatalysis2091
type: Catalysis
controller: Potassium channel subfamily K member 1
controlled: Transport10930
control type: ACTIVATION

Aldosterone
type: SmallMolecule
cellular location: Cytosol
entity reference: Aldosterone

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Reference/CAS 57-13-6
type: UnificationXref
id: 57-13-6
db: CAS

Amiloride-sensitive sodium channel subunit delta
type: Protein
cellular location: Nucleus
entity reference: Amiloride-sensitive sodium channel subunit delta

Transport10958
type: Transport
participant stoichiometry: BoundComplex/16 Cytosol/Stoichiometry/1.0
participant stoichiometry: BoundComplex/16 Nucleus/Stoichiometry/1.0
right: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
spontaneous: false
left: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
name: Active Mineralocorticoid receptor (Homo sapiens) + Aldosterone (→) Transport: Homo sapiens, Kidney, Cytosol to Homo sapiens, Kidney, Nucleus
conversion direction: LEFT_TO_RIGHT

BiologicalState794
type: BioSource
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Kidney
tissue: Kidney

ChemicalStructure/PW C000458
type: ChemicalStructure
structure format: SMILES
structure data: [Na+]

ChemicalStructure/PW C000457
type: ChemicalStructure
structure format: SMILES
structure data: [K+]

Compound/PW C001420 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit alpha-3
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit alpha-3

ProteinComplex/545 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Sodium/potassium ATPase
stoichiometric coefficient: 1.0

Compound/PW C000457 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit alpha-1
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit alpha-1

Reference/UniProt P05023
type: UnificationXref
id: P05023
db: UniProt

Reference/ChemSpider 5628
type: UnificationXref
id: 5628
db: ChemSpider

Sodium/potassium-transporting ATPase subunit beta-3
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit beta-3

Reference/UniProt P05026
type: UnificationXref
id: P05026
db: UniProt

Reference/UniProt P14415
type: UnificationXref
id: P14415
db: UniProt

Reference/KEGG Compound C01780
type: UnificationXref
id: C01780
db: KEGG Compound

Compound/PW C000026 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Aldosterone
stoichiometric coefficient: 1.0

Spironolactone Action Pathway (New)
type: Pathway
pathway order: PathwayInteraction/3250
pathway order: PathwayInteraction/4393
pathway order: PathwayTransport/2522068
pathway order: PathwayTransport/2522067
pathway order: PathwayTransport/2522069
pathway order: PathwayTransport/2522071
pathway order: PathwayTransport/2522070
pathway order: PathwayTransport/2522073
pathway order: PathwayTransport/2531345
pathway order: PathwayTransport/2522072
pathway order: PathwayTransport/2531344
pathway order: PathwayTransport/2522074
pathway order: PathwayTransport/2522066
pathway order: PathwayInteraction/3249
pathway order: PathwayEnzymeReaction/2522065
pathway order: PathwayEnzymeReaction/2522064
pathway component: Interaction4393
pathway component: TransportCatalysis1532
pathway component: Interaction3250
pathway component: Transport10958
pathway component: Transport10930
pathway component: Reaction213252
pathway component: TransportCatalysis33
pathway component: Transport10965
pathway component: Reaction213253
pathway component: TransportCatalysis32
pathway component: Transport10966
pathway component: Transport15529
pathway component: Transport55
pathway component: Transport10362
pathway component: Transport62
pathway component: TransportCatalysis2091
pathway component: Interaction3249
pathway component: Transport15530
pathway component: Transport106
pathway component: Transport303
xref: Reference/SMPDB SMP0126006
xref: Reference/PathWhiz PW127580
organism: Homo sapiens

Sodium/potassium ATPase
type: DnaReference
organism: BiologicalState794

Reference/ChemSpider 1143
type: UnificationXref
id: 1143
db: ChemSpider

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Compound/PW C000026 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Aldosterone
stoichiometric coefficient: 1.0

Reference/BioCyc UREA
type: UnificationXref
id: UREA
db: BioCyc

Sodium/potassium ATPase
type: Complex
cellular location: Cytosol
component: Sodium/potassium-transporting ATPase subunit beta-3
component: Sodium/potassium-transporting ATPase subunit alpha-2
component: ATPase, (Na+)/K+ transporting, beta 4 polypeptide
component: Sodium/potassium-transporting ATPase subunit gamma
component: Sodium/potassium-transporting ATPase subunit alpha-3
component: Sodium/potassium-transporting ATPase subunit beta-1
component: Sodium/potassium-transporting ATPase subunit alpha-1
component: Sodium/potassium-transporting ATPase subunit alpha-4
component: Sodium/potassium-transporting ATPase subunit beta-2

Amiloride-sensitive sodium channel subunit alpha
type: Protein
cellular location: Nucleus
entity reference: Amiloride-sensitive sodium channel subunit alpha

ATPase, (Na+)/K+ transporting, beta 4 polypeptide
type: ProteinReference
xref: Reference/UniProt B7ZKV8
organism: BiologicalState794

Reference/PubChem-compound 24758425
type: UnificationXref
id: 24758425
db: PubChem-compound

Reference/PubChem-compound 813
type: UnificationXref
id: 813
db: PubChem-compound

Aquaporin-3
type: Protein
entity reference: Aquaporin-3

Transport10930
type: Transport
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Cytosol/Stoichiometry/1.0
right: Potassium
spontaneous: true
left: Potassium
name: Potassium (←) Transport: Homo sapiens, Kidney, Cytosol to Homo sapiens, Extracellular Space
conversion direction: REVERSIBLE

Spironolactone
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Spironolactone

Reaction213252
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/583 Nucleus/Stoichiometry/1.0
participant stoichiometry: DNA/349 Nucleus/Stoichiometry/1.0
right: Epithelial Sodium Channel
spontaneous: false
left: Epithelial sodium channel
name: Epithelial sodium channel → Epithelial Sodium Channel
conversion direction: LEFT_TO_RIGHT

Aldosterone
type: SmallMolecule
entity reference: Aldosterone

Reaction213253
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/545 Nucleus/Stoichiometry/1.0
participant stoichiometry: DNA/350 Nucleus/Stoichiometry/1.0
right: Sodium/potassium ATPase
spontaneous: false
left: Sodium/potassium ATPase
name: Sodium/potassium ATPase → Sodium/potassium ATPase
conversion direction: LEFT_TO_RIGHT

ProteinComplex/672/Stoichiometry/1.0
type: Stoichiometry
physical entity: Active Mineralocorticoid receptor
stoichiometric coefficient: 1.0

Urea
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Urea

ProteinComplex/672 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Active Mineralocorticoid receptor
stoichiometric coefficient: 1.0

Spironolactone
type: SmallMolecule
cellular location: Cytosol
entity reference: Spironolactone

Sodium/potassium-transporting ATPase subunit alpha-2
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit alpha-2

Sodium/potassium-transporting ATPase subunit gamma
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit gamma

Amiloride-sensitive sodium channel subunit delta
type: Protein
entity reference: Amiloride-sensitive sodium channel subunit delta

ChemicalStructure/PW C000200
type: ChemicalStructure
structure format: SMILES
structure data: NC(N)=O

Amiloride-sensitive sodium channel subunit beta
type: Protein
entity reference: Amiloride-sensitive sodium channel subunit beta

Epithelial Sodium Channel
type: Complex
cellular location: Cytosol
component: Amiloride-sensitive sodium channel subunit delta
component: Amiloride-sensitive sodium channel subunit beta
component: Amiloride-sensitive sodium channel subunit gamma
component: Amiloride-sensitive sodium channel subunit alpha

Sodium/potassium-transporting ATPase subunit gamma
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit gamma

Sodium/potassium-transporting ATPase subunit alpha-4
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit alpha-4

Reference/BioCyc ALDOSTERONE
type: UnificationXref
id: ALDOSTERONE
db: BioCyc

Reference/UniProt P37088
type: UnificationXref
id: P37088
db: UniProt

Reference/KEGG Compound C00238
type: UnificationXref
id: C00238
db: KEGG Compound

Sodium/potassium-transporting ATPase subunit gamma
type: ProteinReference
xref: Reference/UniProt P54710
organism: BiologicalState794

Aquaporin-2
type: Protein
entity reference: Aquaporin-2

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

Reference/ChemSpider 24774738
type: UnificationXref
id: 24774738
db: ChemSpider

Kidney
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000671

Spironolactone
type: SmallMolecule
entity reference: Spironolactone

Transport10362
type: Transport
participant stoichiometry: Compound/PW C001420 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/1.0
right: Water
spontaneous: false
left: Water
name: Water (→) Transport: Homo sapiens, Cell, Extracellular Space to Homo sapiens, Cell, Cytosol
conversion direction: LEFT_TO_RIGHT

ProteinComplex/583 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Epithelial Sodium Channel
stoichiometric coefficient: 1.0

Reference/KEGG Compound C07310
type: UnificationXref
id: C07310
db: KEGG Compound

Amiloride-sensitive sodium channel subunit beta
type: Protein
cellular location: Cytosol
entity reference: Amiloride-sensitive sodium channel subunit beta

TransportCatalysis1532
type: Catalysis
controller: Aquaporin-2
controlled: Transport10362
control type: ACTIVATION

Sodium/potassium-transporting ATPase subunit beta-3
type: ProteinReference
xref: Reference/UniProt P54709
organism: BiologicalState794

Compound/PW C000458 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Sodium
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Reference/CAS 24203-36-9
type: UnificationXref
id: 24203-36-9
db: CAS

Reference/KEGG Compound C01330
type: UnificationXref
id: C01330
db: KEGG Compound

Urea
type: SmallMolecule
cellular location: Cytosol
entity reference: Urea

Reference/UniProt P13637
type: UnificationXref
id: P13637
db: UniProt

Transport55
type: Transport
participant stoichiometry: Compound/PW C001420 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/1.0
right: Water
spontaneous: false
left: Water
name: Water (→) Transport: Homo sapiens, Cell, Cytosol to Homo sapiens, Cell, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Transport62
type: Transport
participant stoichiometry: Compound/PW C000458 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000458 Cytosol/Stoichiometry/1.0
right: Sodium
spontaneous: false
left: Sodium
name: Sodium (→) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Cell, Cytosol
conversion direction: LEFT_TO_RIGHT

Reference/HMDB HMDB0000586
type: UnificationXref
id: HMDB0000586
db: HMDB

ChemicalStructure/PW C000026
type: ChemicalStructure
structure format: SMILES

Reference/HMDB HMDB0000588
type: UnificationXref
id: HMDB0000588
db: HMDB

Reference/GENE ONTOLOGY GO 0005634
type: UnificationXref
id: GO:0005634
db: GENE ONTOLOGY

ATPase, (Na+)/K+ transporting, beta 4 polypeptide
type: Protein
cellular location: Cytosol
entity reference: ATPase, (Na+)/K+ transporting, beta 4 polypeptide

Reference/UniProt P54710
type: UnificationXref
id: P54710
db: UniProt

Reference/PubChem-compound 5833
type: UnificationXref
id: 5833
db: PubChem-compound

Mineralocorticoid receptor
type: Protein
cellular location: Cytosol
entity reference: Mineralocorticoid receptor

Amiloride-sensitive sodium channel subunit alpha
type: Protein
entity reference: Amiloride-sensitive sodium channel subunit alpha

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Mineralocorticoid receptor
type: Protein
entity reference: Mineralocorticoid receptor

Compound/PW C008934 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Spironolactone
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit beta-2
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit beta-2

Reference/UniProt Q13733
type: UnificationXref
id: Q13733
db: UniProt

Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Epithelial Sodium Channel
type: Complex
component: Amiloride-sensitive sodium channel subunit gamma
component: Amiloride-sensitive sodium channel subunit delta
component: Amiloride-sensitive sodium channel subunit beta
component: Amiloride-sensitive sodium channel subunit alpha

Amiloride-sensitive sodium channel subunit gamma
type: Protein
entity reference: Amiloride-sensitive sodium channel subunit gamma

Sodium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Sodium

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
type: Complex
component stoichiometry: ProteinComplex/672 Nucleus/Stoichiometry/1.0
component stoichiometry: Compound/PW C000026 Nucleus/Stoichiometry/1.0
cellular location: Nucleus
component: Aldosterone
component: Active Mineralocorticoid receptor

Sodium/potassium-transporting ATPase subunit beta-1
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit beta-1

Reference/ChEBI CHEBI 29101
type: UnificationXref
id: CHEBI:29101
db: ChEBI

Reference/ChEBI CHEBI 29103
type: UnificationXref
id: CHEBI:29103
db: ChEBI

Sodium/potassium ATPase
type: Complex
cellular location: Nucleus
component: Sodium/potassium-transporting ATPase subunit beta-1
component: Sodium/potassium-transporting ATPase subunit beta-3
component: Sodium/potassium-transporting ATPase subunit alpha-2
component: ATPase, (Na+)/K+ transporting, beta 4 polypeptide
component: Sodium/potassium-transporting ATPase subunit beta-2
component: Sodium/potassium-transporting ATPase subunit gamma
component: Sodium/potassium-transporting ATPase subunit alpha-3
component: Sodium/potassium-transporting ATPase subunit alpha-1
component: Sodium/potassium-transporting ATPase subunit alpha-4

Protein/2032/Stoichiometry/1.0
type: Stoichiometry
physical entity: Mineralocorticoid receptor
stoichiometric coefficient: 1.0

Compound/PW C000458 Cytosol/Stoichiometry/3.0
type: Stoichiometry
physical entity: Sodium
stoichiometric coefficient: 3.0

BoundComplex/16 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
stoichiometric coefficient: 1.0

Reference/SMPDB SMP0126006
type: UnificationXref
id: SMP0126006
db: SMPDB

Reference/UniProt P08235
type: UnificationXref
id: P08235
db: UniProt

Amiloride-sensitive sodium channel subunit delta
type: ProteinReference
xref: Reference/UniProt P51172
organism: BiologicalState794

Sodium/potassium-transporting ATPase subunit beta-1
type: ProteinReference
xref: Reference/UniProt P05026
organism: BiologicalState794

Reference/UniProt P41181
type: UnificationXref
id: P41181
db: UniProt

Aquaporin-3
type: ProteinReference
xref: Reference/UniProt Q92482

Amiloride-sensitive sodium channel subunit beta
type: ProteinReference
xref: Reference/UniProt P51168
organism: BiologicalState794

ProteinComplex/583 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Epithelial Sodium Channel
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit beta-2
type: ProteinReference
xref: Reference/UniProt P14415
organism: BiologicalState794

Reference/UniProt B7ZKV8
type: UnificationXref
id: B7ZKV8
db: UniProt

Reference/ChemSpider 899
type: UnificationXref
id: 899
db: ChemSpider

Sodium/potassium-transporting ATPase subunit beta-3
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit beta-3

Sodium/potassium ATPase
type: Dna
cellular location: Nucleus
entity reference: Sodium/potassium ATPase

Potassium channel subfamily K member 1
type: Protein
entity reference: Potassium channel subfamily K member 1

Potassium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Potassium

Amiloride-sensitive sodium channel subunit gamma
type: Protein
cellular location: Nucleus
entity reference: Amiloride-sensitive sodium channel subunit gamma

Compound/PW C000458 Extracellular Space/Stoichiometry/3.0
type: Stoichiometry
physical entity: Sodium
stoichiometric coefficient: 3.0

Compound/PW C000026/Stoichiometry/1.0
type: Stoichiometry
physical entity: Aldosterone
stoichiometric coefficient: 1.0

Compound/PW C000457 Extracellular Space/Stoichiometry/2.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 2.0

Aquaporin-2
type: ProteinReference
xref: Reference/UniProt P41181

Reference/BioCyc K+
type: UnificationXref
id: K%2b
db: BioCyc

Compound/PW C000200 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Urea
stoichiometric coefficient: 1.0

Reference/UniProt P54709
type: UnificationXref
id: P54709
db: UniProt

Reference/UniProt Q92482
type: UnificationXref
id: Q92482
db: UniProt

Urea
type: SmallMoleculeReference
xref: Reference/BioCyc UREA
xref: Reference/CAS 57-13-6
xref: Reference/KEGG Compound C00086
xref: Reference/HMDB HMDB0000294
xref: Reference/ChemSpider 1143
xref: Reference/PubChem-compound 1176
xref: Reference/ChEBI CHEBI 16199
chemical formula: CH4N2O
molecular weight: 60.032364
structure: ChemicalStructure/PW C000200

DNA/349 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Epithelial sodium channel
stoichiometric coefficient: 1.0

Reference/CAS 17341-25-2
type: UnificationXref
id: 17341-25-2
db: CAS

Aldosterone
type: SmallMolecule
cellular location: Nucleus
entity reference: Aldosterone

Epithelial sodium channel
type: Dna
cellular location: Nucleus
entity reference: Epithelial sodium channel

Protein/2032/Stoichiometry/2.0
type: Stoichiometry
physical entity: Mineralocorticoid receptor
stoichiometric coefficient: 2.0

Sodium/potassium-transporting ATPase subunit alpha-4
type: ProteinReference
xref: Reference/UniProt Q13733
organism: BiologicalState794

Homo sapiens
type: BioSource
xref: Reference/TAXONOMY 9606

Potassium
type: SmallMolecule
cellular location: Cytosol
entity reference: Potassium

TransportCatalysis33
type: Catalysis
controller: Epithelial Sodium Channel
controlled: Transport62
control type: ACTIVATION

Sodium/potassium-transporting ATPase subunit alpha-1
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit alpha-1

TransportCatalysis32
type: Catalysis
controller: Aquaporin-3
controlled: Transport55
control type: ACTIVATION

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Sodium/potassium-transporting ATPase subunit alpha-1
type: ProteinReference
xref: Reference/UniProt P05023
organism: BiologicalState794

Reference/ChEBI CHEBI 16199
type: UnificationXref
id: CHEBI:16199
db: ChEBI

Reference/HMDB HMDB0000037
type: UnificationXref
id: HMDB0000037
db: HMDB

Interaction3249
type: MolecularInteraction
interaction type: direct_interaction
name: Active Mineralocorticoid receptor (Homo sapiens) + Aldosterone activates Epithelial sodium channel
participant: Epithelial sodium channel
participant: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone

Amiloride-sensitive sodium channel subunit beta
type: Protein
cellular location: Nucleus
entity reference: Amiloride-sensitive sodium channel subunit beta

Spironolactone
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0014565
xref: Reference/KEGG Compound C07310
xref: Reference/ChemSpider 5628
xref: Reference/CAS 52-01-7
xref: Reference/PubChem-compound 5833
xref: Reference/ChEBI CHEBI 9241
chemical formula: C24H32O4S
molecular weight: 416.20212
structure: ChemicalStructure/PW C008934

Reference/ChemSpider 791
type: UnificationXref
id: 791
db: ChemSpider

Active Mineralocorticoid receptor
type: Complex
component stoichiometry: Protein/2032 Nucleus/Stoichiometry/2.0
cellular location: Nucleus
component: Mineralocorticoid receptor

Water
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Water

Water
type: SmallMolecule
cellular location: Cytosol
entity reference: Water

Amiloride-sensitive sodium channel subunit alpha
type: ProteinReference
xref: Reference/UniProt P37088
organism: BiologicalState794

Reference/HMDB HMDB0014565
type: UnificationXref
id: HMDB0014565
db: HMDB

Epithelial Sodium Channel
type: Complex
cellular location: Nucleus
component: Amiloride-sensitive sodium channel subunit alpha
component: Amiloride-sensitive sodium channel subunit beta
component: Amiloride-sensitive sodium channel subunit delta
component: Amiloride-sensitive sodium channel subunit gamma

ProteinComplex/672 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Active Mineralocorticoid receptor
stoichiometric coefficient: 1.0

Compound/PW C008934/Stoichiometry/1.0
type: Stoichiometry
physical entity: Spironolactone
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit alpha-2
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit alpha-2

Sodium/potassium-transporting ATPase subunit alpha-4
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit alpha-4

Mineralocorticoid receptor
type: ProteinReference
xref: Reference/UniProt P08235
organism: BiologicalState794

Amiloride-sensitive sodium channel subunit gamma
type: ProteinReference
xref: Reference/UniProt P51170
organism: BiologicalState794

Compound/PW C008934 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Spironolactone
stoichiometric coefficient: 1.0

Compound/PW C001420 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Amiloride-sensitive sodium channel subunit alpha
type: Protein
cellular location: Cytosol
entity reference: Amiloride-sensitive sodium channel subunit alpha

Transport303
type: Transport
participant stoichiometry: Compound/PW C000458 Cytosol/Stoichiometry/3.0
participant stoichiometry: Compound/PW C000458 Extracellular Space/Stoichiometry/3.0
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/2.0
participant stoichiometry: Compound/PW C000457 Cytosol/Stoichiometry/2.0
right: Sodium
right: Potassium
spontaneous: false
left: Sodium
left: Potassium
name: Sodium (→), Potassium (←) Transport: Homo sapiens, Cell, Cytosol to Homo sapiens, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Reference/CAS 52-39-1
type: UnificationXref
id: 52-39-1
db: CAS

Reference/TAXONOMY 9606
type: UnificationXref
id: 9606
db: TAXONOMY

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Sodium
type: SmallMolecule
cellular location: Cytosol
entity reference: Sodium

Compound/PW C000458 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Sodium
stoichiometric coefficient: 1.0

Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
type: Complex
component stoichiometry: ProteinComplex/672 Cytosol/Stoichiometry/1.0
component stoichiometry: Compound/PW C000026 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Aldosterone
component: Active Mineralocorticoid receptor

Reference/PubChem-compound 1176
type: UnificationXref
id: 1176
db: PubChem-compound

Active Mineralocorticoid receptor
type: Complex
component stoichiometry: Protein/2032 Cytosol/Stoichiometry/2.0
cellular location: Cytosol
component: Mineralocorticoid receptor

Protein/2032 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Mineralocorticoid receptor
stoichiometric coefficient: 2.0

ChemicalStructure/PW C008934
type: ChemicalStructure
structure format: SMILES

Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
type: Complex
component stoichiometry: Compound/PW C000026/Stoichiometry/1.0
component stoichiometry: ProteinComplex/672/Stoichiometry/1.0
component: Aldosterone
component: Active Mineralocorticoid receptor

Transport15530
type: Transport
participant stoichiometry: Compound/PW C008934/Stoichiometry/1.0
participant stoichiometry: Compound/PW C008934 Extracellular Space/Stoichiometry/1.0
right: Spironolactone
spontaneous: false
left: Spironolactone
name: Spironolactone (→) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Blood Vessel
conversion direction: LEFT_TO_RIGHT

ATPase, (Na+)/K+ transporting, beta 4 polypeptide
type: Protein
cellular location: Nucleus
entity reference: ATPase, (Na+)/K+ transporting, beta 4 polypeptide

Reference/CAS 52-01-7
type: UnificationXref
id: 52-01-7
db: CAS

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

Reference/HMDB HMDB0000294
type: UnificationXref
id: HMDB0000294
db: HMDB

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Interaction3250
type: MolecularInteraction
interaction type: direct_interaction
name: Active Mineralocorticoid receptor (Homo sapiens) + Aldosterone activates Sodium/potassium ATPase
participant: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
participant: Sodium/potassium ATPase

ProteinComplex/545 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Sodium/potassium ATPase
stoichiometric coefficient: 1.0

Epithelial sodium channel
type: DnaReference
organism: BiologicalState794

DNA/350 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Sodium/potassium ATPase
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit alpha-3
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit alpha-3

Reference/KEGG Compound C00086
type: UnificationXref
id: C00086
db: KEGG Compound

Amiloride-sensitive sodium channel subunit gamma
type: Protein
cellular location: Cytosol
entity reference: Amiloride-sensitive sodium channel subunit gamma

Transport15529
type: Transport
participant stoichiometry: Compound/PW C008934/Stoichiometry/1.0
participant stoichiometry: Compound/PW C008934 Cytosol/Stoichiometry/1.0
right: Spironolactone
spontaneous: false
left: Spironolactone
name: Spironolactone (→) Transport: Homo sapiens, Blood Vessel to Homo sapiens, Kidney, Cytosol
conversion direction: LEFT_TO_RIGHT

Potassium channel subfamily K member 1
type: ProteinReference

Spironolactone_Mineralocorticoid receptor (Homo sapiens)
type: Complex
component stoichiometry: Protein/2032/Stoichiometry/1.0
component stoichiometry: Compound/PW C008934/Stoichiometry/1.0
component: Spironolactone
component: Mineralocorticoid receptor

BoundComplex/16 Nucleus/Stoichiometry/1.0
type: Stoichiometry
physical entity: Active Mineralocorticoid receptor (Homo sapiens)_Aldosterone
stoichiometric coefficient: 1.0

Reference/ChEBI CHEBI 27584
type: UnificationXref
id: CHEBI:27584
db: ChEBI

Amiloride-sensitive sodium channel subunit delta
type: Protein
cellular location: Cytosol
entity reference: Amiloride-sensitive sodium channel subunit delta

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Reference/UniProt P50993
type: UnificationXref
id: P50993
db: UniProt

Reference/UniProt P51168
type: UnificationXref
id: P51168
db: UniProt

Nucleus
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005634

Reference/UniProt P51172
type: UnificationXref
id: P51172
db: UniProt

Reference/UniProt P51170
type: UnificationXref
id: P51170
db: UniProt

Sodium
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000588
xref: Reference/PubChem-compound 923
xref: Reference/KEGG Compound C01330
xref: Reference/ChEBI CHEBI 29101
xref: Reference/ChemSpider 899
xref: Reference/CAS 17341-25-2
chemical formula: Na
molecular weight: 22.989769
structure: ChemicalStructure/PW C000458

Sodium/potassium-transporting ATPase subunit alpha-2
type: ProteinReference
xref: Reference/UniProt P50993
organism: BiologicalState794

Potassium
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00238
xref: Reference/HMDB HMDB0000586
xref: Reference/PubChem-compound 813
xref: Reference/ChemSpider 791
xref: Reference/CAS 24203-36-9
xref: Reference/BioCyc K+
xref: Reference/ChEBI CHEBI 29103
chemical formula: K
molecular weight: 38.963707
structure: ChemicalStructure/PW C000457

Protein/2032 Nucleus/Stoichiometry/2.0
type: Stoichiometry
physical entity: Mineralocorticoid receptor
stoichiometric coefficient: 2.0

Sodium/potassium-transporting ATPase subunit beta-2
type: Protein
cellular location: Cytosol
entity reference: Sodium/potassium-transporting ATPase subunit beta-2

Reference/BRENDA TISSUE ONTOLOGY BTO 0000671
type: UnificationXref
id: BTO:0000671
db: BRENDA TISSUE ONTOLOGY

Compound/PW C000200 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Urea
stoichiometric coefficient: 1.0

Sodium/potassium-transporting ATPase subunit beta-1
type: Protein
cellular location: Nucleus
entity reference: Sodium/potassium-transporting ATPase subunit beta-1

Mineralocorticoid receptor
type: Protein
cellular location: Nucleus
entity reference: Mineralocorticoid receptor

Transport106
type: Transport
participant stoichiometry: Compound/PW C000200 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000200 Cytosol/Stoichiometry/1.0
right: Urea
spontaneous: false
left: Urea
name: Urea (→) Transport: Homo sapiens, Cell, Cytosol to Homo sapiens, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Reference/PathWhiz PW127580
type: UnificationXref
id: PW127580
db: PathWhiz

Compound/PW C000457 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 2.0