Protein/23969/Stoichiometry/4.0
type: Stoichiometry
physical entity: Intermediate conductance calcium-activated potassium channel protein 4
stoichiometric coefficient: 4.0

Acetyl-CoA
type: SmallMoleculeReference
xref: Reference/BioCyc ACETYL-COA
xref: Reference/PubChem-compound 444493
xref: Reference/HMDB HMDB0001206
xref: Reference/ChemSpider 392413
xref: Reference/CAS 72-89-9
xref: Reference/ChEBI CHEBI 15351
xref: Reference/KEGG Compound C00024
chemical formula: C23H38N7O17P3S
molecular weight: 809.1258
structure: ChemicalStructure/PW C000940

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

ATP-binding cassette sub-family C member 9
type: Protein
entity reference: ATP-binding cassette sub-family C member 9

BiologicalState789
type: BioSource
cell type: Neuron
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Brain, Neuron
tissue: Brain

Protein kinase A
type: Complex
component stoichiometry: Protein/1315 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/3359 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/3358 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/1990 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/1134 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/3357 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/1262 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: cAMP-dependent protein kinase catalytic subunit alpha
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit beta
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit gamma
component: cAMP-dependent protein kinase type I-beta regulatory subunit

direct_interaction
type: InteractionVocabulary

Potassium voltage-gated channel subfamily A member 1
type: Protein
entity reference: Potassium voltage-gated channel subfamily A member 1

Reference/HMDB HMDB0000097
type: UnificationXref
id: HMDB0000097
db: HMDB

Acetylcholine
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15355
xref: Reference/CAS 51-84-3
xref: Reference/HMDB HMDB0000895
xref: Reference/ChemSpider 182
xref: Reference/KEGG Compound C01996
xref: Reference/BioCyc ACETYLCHOLINE
xref: Reference/PubChem-compound 187
chemical formula: C7H16NO2
molecular weight: 146.1181
structure: ChemicalStructure/PW C000714

Voltage-dependent L-type calcium channel subunit alpha-1C
type: Protein
cellular location: Cytosol
entity reference: Voltage-dependent L-type calcium channel subunit alpha-1C

Brain
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000142

Calcium
type: SmallMolecule
cellular location: Cytosol
entity reference: Calcium

Reference/BioCyc ATP
type: UnificationXref
id: ATP
db: BioCyc

Reference/UniProt O15554
type: UnificationXref
id: O15554
db: UniProt

ChemicalStructure/PW C097005
type: ChemicalStructure
structure format: SMILES

Compound/PW C000065 Synapse/Stoichiometry/1.0
type: Stoichiometry
physical entity: Choline
stoichiometric coefficient: 1.0

ATP-sensitive inward rectifier potassium channel (IKATP)
type: Complex
component: ATP-sensitive inward rectifier potassium channel 8
component: ATP-binding cassette sub-family C member 8
component: ATP-sensitive inward rectifier potassium channel 11
component: ATP-binding cassette sub-family C member 9

cAMP-dependent protein kinase catalytic subunit gamma
type: Protein
entity reference: cAMP-dependent protein kinase catalytic subunit gamma

Compound/PW C000353/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Compound/PW C000457 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Compound/PW C000065/Stoichiometry/1.0
type: Stoichiometry
physical entity: Choline
stoichiometric coefficient: 1.0

ReactionCatalysis202856
type: Catalysis
controller: Myosin light chain kinase, smooth muscle
controlled: 2.7.11.18
control type: ACTIVATION

Intermediate conductance calcium-activated potassium channel protein 4
type: Complex
component stoichiometry: Protein/23969/Stoichiometry/4.0
component: Intermediate conductance calcium-activated potassium channel protein 4

Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Reference/ChemSpider 5742
type: UnificationXref
id: 5742
db: ChemSpider

Acetylcholine
type: SmallMolecule
entity reference: Acetylcholine

Reference/KEGG Compound C00575
type: UnificationXref
id: C00575
db: KEGG Compound

Reference/ChEBI CHEBI 16595
type: UnificationXref
id: CHEBI:16595
db: ChEBI

Sarcoplasmic Reticulum
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0016529

cAMP-dependent protein kinase catalytic subunit alpha
type: Protein
entity reference: cAMP-dependent protein kinase catalytic subunit alpha

Reference/UniProt Q9NQ66
type: UnificationXref
id: Q9NQ66
db: UniProt

SubPathwayInteraction120651
type: Interaction
name: SubPathwayReaction
name: SubPathway120651Reaction
participant: Myosin light chain 3

Reference/BRENDA TISSUE ONTOLOGY BTO 0000887
type: UnificationXref
id: BTO:0000887
db: BRENDA TISSUE ONTOLOGY

Reference/UniProt Q13936
type: UnificationXref
id: Q13936
db: UniProt

SubPathwayInteraction120652
type: Interaction
name: SubPathway120652Reaction
name: SubPathwayReaction
participant: Myosin light chain 3

Reference/PubChem-compound 813
type: UnificationXref
id: 813
db: PubChem-compound

Inositol 1,4,5-trisphosphate receptor type 1
type: Protein
entity reference: Inositol 1,4,5-trisphosphate receptor type 1

Reference/ChemSpider 5851
type: UnificationXref
id: 5851
db: ChemSpider

Compound/Generic402 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol-4,5-bisphosphate
stoichiometric coefficient: 1.0

Protein/1597 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calmodulin-1
stoichiometric coefficient: 1.0

ChemicalStructure/PW C001099
type: ChemicalStructure
structure format: SMILES

Reference/UniProt P62140
type: UnificationXref
id: P62140
db: UniProt

Compound/PW C000714 Exocytic Vesicle/Stoichiometry/1.0
type: Stoichiometry
physical entity: Acetylcholine
stoichiometric coefficient: 1.0

L-type calcium channel
type: Complex
component: Voltage-dependent calcium channel subunit alpha-2/delta-1
component: Voltage-dependent L-type calcium channel subunit alpha-1C
component: Voltage-dependent L-type calcium channel subunit beta-1

Protein/2126/Stoichiometry/1.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate receptor type 1
stoichiometric coefficient: 1.0

Reference/UniProt P17252
type: UnificationXref
id: P17252
db: UniProt

Diacylglycerol
type: SmallMolecule
cellular location: Cytosol
entity reference: Diacylglycerol

Compound/PW C113474/Stoichiometry/1.0
type: Stoichiometry
physical entity: Amifampridine
stoichiometric coefficient: 1.0

Reference/KEGG Compound C00238
type: UnificationXref
id: C00238
db: KEGG Compound

Protein/3359 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-beta regulatory subunit
stoichiometric coefficient: 1.0

Compound/PW C000414 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Voltage-dependent L-type calcium channel subunit beta-1
type: Protein
cellular location: Cytosol
entity reference: Voltage-dependent L-type calcium channel subunit beta-1

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

Reference/KEGG Compound C00114
type: UnificationXref
id: C00114
db: KEGG Compound

Acetylcholine
type: SmallMolecule
cellular location: post-synaptic membrane
entity reference: Acetylcholine

Choline O-acetyltransferase
type: ProteinReference
xref: Reference/UniProt P28329
organism: BiologicalState789

Adenosine triphosphate
type: SmallMolecule
entity reference: Adenosine triphosphate

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
type: Protein
cellular location: Cytosol
entity reference: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1

Transport13992
type: Transport
participant stoichiometry: Compound/PW C000714 Synapse/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000714 Exocytic Vesicle/Stoichiometry/1.0
right: Acetylcholine
spontaneous: false
left: Acetylcholine
name: Acetylcholine (→) Transport: Homo sapiens, Brain, Neuron, Exocytic Vesicle to Homo sapiens, Brain, Neuron, Synapse
conversion direction: LEFT_TO_RIGHT

Transport13991
type: Transport
participant stoichiometry: Compound/PW C000714 Exocytic Vesicle/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000714 Cytosol/Stoichiometry/1.0
right: Acetylcholine
spontaneous: false
left: Acetylcholine
name: Acetylcholine (→) Transport: Homo sapiens, Brain, Neuron, Cytosol to Homo sapiens, Brain, Neuron, Exocytic Vesicle
conversion direction: LEFT_TO_RIGHT

Transport13993
type: Transport
participant stoichiometry: Compound/PW C000065 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000065 Synapse/Stoichiometry/1.0
right: Choline
spontaneous: false
left: Choline
name: Choline (→) Transport: Homo sapiens, Brain, Neuron, Synapse to Homo sapiens, Brain, Neuron, Cytosol
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 6026790
type: UnificationXref
id: 6026790
db: PubChem-compound

Compound/PW C000353 Sarcoplasmic Reticulum/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Reference/UniProt P28329
type: UnificationXref
id: P28329
db: UniProt

BiologicalState73
type: BioSource
cell type: Neuron
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Neuron

Reference/BioCyc inositol 1,4,5-trisphosphate
type: UnificationXref
id: inositol_1,4,5-trisphosphate
db: BioCyc

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type I-alpha regulatory subunit

Choline
type: SmallMolecule
cellular location: Cytosol
entity reference: Choline

Reference/CAS 54-96-6
type: UnificationXref
id: 54-96-6
db: CAS

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type II-alpha regulatory subunit

Reference/CAS 24203-36-9
type: UnificationXref
id: 24203-36-9
db: CAS

cAMP-dependent protein kinase type I-beta regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type I-beta regulatory subunit

Intermediate conductance calcium-activated potassium channel protein 4
type: ProteinReference
xref: Reference/UniProt O15554

cAMP-dependent protein kinase type II-beta regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type II-beta regulatory subunit

Reference/KEGG Compound C00002
type: UnificationXref
id: C00002
db: KEGG Compound

Reference/ChemSpider 388562
type: UnificationXref
id: 388562
db: ChemSpider

Reference/UniProt Q09470
type: UnificationXref
id: Q09470
db: UniProt

Reference/ChEBI CHEBI 17489
type: UnificationXref
id: CHEBI:17489
db: ChEBI

Coenzyme A
type: SmallMolecule
cellular location: Cytosol
entity reference: Coenzyme A

Reference/CELL TYPE ONTOLOGY CL 0000540
type: UnificationXref
id: CL:0000540
db: CELL TYPE ONTOLOGY

Voltage-dependent L-type calcium channel subunit alpha-1C
type: ProteinReference
xref: Reference/UniProt Q13936

Reference/KEGG Compound C00010
type: UnificationXref
id: C00010
db: KEGG Compound

Serine/threonine-protein phosphatase PP1-beta catalytic subunit
type: ProteinReference
xref: Reference/UniProt P62140
organism: BiologicalState1056

ProteinComplex/7708/Stoichiometry/1.0
type: Stoichiometry
physical entity: Protein kinase A
stoichiometric coefficient: 1.0

Extracellular Space
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005615

Phosphatidylinositol 4,5-bisphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Phosphatidylinositol 4,5-bisphosphate

Protein/216 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
stoichiometric coefficient: 1.0

Amifampridine
type: SmallMolecule
entity reference: Amifampridine

Reference/CAS 14127-61-8
type: UnificationXref
id: 14127-61-8
db: CAS

ATP-sensitive inward rectifier potassium channel 11
type: Protein
entity reference: ATP-sensitive inward rectifier potassium channel 11

Calcium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Calcium

Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

Reference/CAS 56-65-5
type: UnificationXref
id: 56-65-5
db: CAS

Phosphatidylinositol 4,5-bisphosphate
type: SmallMoleculeReference
xref: Reference/PubChem-compound 24742074
chemical formula: C47H80O19P3
molecular weight: 1041.4507
structure: ChemicalStructure/PW C097005

Calmodulin-1
type: Protein
cellular location: Cytosol
entity reference: Calmodulin-1

Transport14933
type: Transport
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Cytoplasm/Stoichiometry/1.0
right: Potassium
spontaneous: false
left: Potassium
name: Potassium (→) Transport: Homo sapiens, Cell, Cytoplasm to Homo sapiens, Extracellular Space
conversion direction: LEFT_TO_RIGHT

post-synaptic membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0045211

Reference/KEGG Compound C00024
type: UnificationXref
id: C00024
db: KEGG Compound

Acetylcholinesterase
type: ProteinReference
xref: Reference/UniProt P22303

Reference/BioCyc ACET
type: UnificationXref
id: ACET
db: BioCyc

Reference/ChEBI CHEBI 29108
type: UnificationXref
id: CHEBI:29108
db: ChEBI

Compound/PW C000714/Stoichiometry/1.0
type: Stoichiometry
physical entity: Acetylcholine
stoichiometric coefficient: 1.0

Reference/CAS 60-92-4
type: UnificationXref
id: 60-92-4
db: CAS

Reaction2159
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000414/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000041/Stoichiometry/1.0
right: cAMP
spontaneous: false
left: Adenosine triphosphate
name: Adenosine triphosphate → cAMP
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 29103
type: UnificationXref
id: CHEBI:29103
db: ChEBI

Acetylcholine
type: SmallMolecule
cellular location: Synapse
entity reference: Acetylcholine

ProteinComplex/15522/Stoichiometry/1.0
type: Stoichiometry
physical entity: Intermediate conductance calcium-activated potassium channel protein 4
stoichiometric coefficient: 1.0

Transport14925
type: Transport
participant stoichiometry: Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Lung
conversion direction: LEFT_TO_RIGHT

Reference/KEGG Compound C00033
type: UnificationXref
id: C00033
db: KEGG Compound

Transport14926
type: Transport
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Cytosol/Stoichiometry/1.0
right: Potassium
spontaneous: false
left: Potassium
name: Potassium (→) Transport: Homo sapiens, Cell, Cytosol to Homo sapiens, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Reference/KEGG Compound C01245
type: UnificationXref
id: C01245
db: KEGG Compound

Water
type: SmallMolecule
entity reference: Water

Amifampridine
type: SmallMoleculeReference
xref: Reference/ChemSpider 5705
xref: Reference/CAS 54-96-6
xref: Reference/ChEBI CHEBI 135948
xref: Reference/HMDB HMDB0246022
chemical formula: C5H7N3
molecular weight: 109.063995
structure: ChemicalStructure/PW C113474

Reference/HMDB HMDB0246022
type: UnificationXref
id: HMDB0246022
db: HMDB

Potassium
type: SmallMolecule
cellular location: Extracellular Space
entity reference: Potassium

Diacylglycerol
type: SmallMoleculeReference
xref: Reference/PubChem-compound 6026790
chemical formula: C37H70O5
molecular weight: 594.52234
structure: ChemicalStructure/PW C097908

Diacylglycerol
type: SmallMoleculeReference

cAMP-dependent protein kinase type II-beta regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type II-beta regulatory subunit

Reference/BioCyc K+
type: UnificationXref
id: K%2b
db: BioCyc

ChemicalStructure/PW C001155
type: ChemicalStructure
structure format: SMILES

Reference/GENE ONTOLOGY GO 0045211
type: UnificationXref
id: GO:0045211
db: GENE ONTOLOGY

ChemicalStructure/PW C000065
type: ChemicalStructure
structure format: SMILES
structure data: C[N+](C)(C)CCO

Potassium voltage-gated channel subfamily A member 1
type: Protein
cellular location: Cell Membrane
entity reference: Potassium voltage-gated channel subfamily A member 1

Compound/PW C113474 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Amifampridine
stoichiometric coefficient: 1.0

Reference/CAS 51-84-3
type: UnificationXref
id: 51-84-3
db: CAS

Voltage-dependent calcium channel subunit alpha-2/delta-1
type: ProteinReference
xref: Reference/UniProt P54289

Vesicular acetylcholine transporter
type: ProteinReference
xref: Reference/UniProt Q16572

BoundComplex/506 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium_Calmodulin (Homo sapiens)
stoichiometric coefficient: 1.0

Potassium voltage-gated channel subfamily A member 1
type: Complex
component stoichiometry: Protein/24379/Stoichiometry/4.0
component: Potassium voltage-gated channel subfamily A member 1

Reference/PubChem-compound 305
type: UnificationXref
id: 305
db: PubChem-compound

ChemicalStructure/PW C000029
type: ChemicalStructure
structure format: SMILES
structure data: CC(O)=O

Potassium
type: SmallMolecule
cellular location: Cytosol
entity reference: Potassium
name: SubPathwayActivator

High affinity choline transporter 1
type: ProteinReference
xref: Reference/UniProt Q9GZV3

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Protein/1990/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-alpha regulatory subunit
stoichiometric coefficient: 1.0

Reference/ChemSpider 791
type: UnificationXref
id: 791
db: ChemSpider

Compound/PW C000029/Stoichiometry/1.0
type: Stoichiometry
physical entity: Acetic acid
stoichiometric coefficient: 1.0

Reaction213775
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: BoundComplex/506 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/1597 Cytosol/Stoichiometry/1.0
right: Calcium_Calmodulin (Homo sapiens)
spontaneous: false
left: Calcium
left: Calmodulin-1
name: Calcium + Calmodulin → Calcium + Calmodulin (Homo sapiens)
conversion direction: LEFT_TO_RIGHT

3.1.3.16
type: BiochemicalReaction
participant stoichiometry: Protein/3041 Cytosol/Stoichiometry/1.0
right: Myosin light chain 3
spontaneous: false
EC number: 3.1.3.16
left: Myosin light chain 3
name: Myosin LC-P → Myosin light chain 3
conversion direction: LEFT_TO_RIGHT

Reference/UniProt Q9GZV3
type: UnificationXref
id: Q9GZV3
db: UniProt

Adenosine triphosphate
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15422
xref: Reference/PubChem-compound 5957
xref: Reference/HMDB HMDB0000538
xref: Reference/CAS 56-65-5
xref: Reference/ChemSpider 5742
xref: Reference/BioCyc ATP
xref: Reference/KEGG Compound C00002
chemical formula: C10H16N5O13P3
molecular weight: 506.99576
structure: ChemicalStructure/PW C000414

Interaction3231
type: MolecularInteraction
interaction type: direct_interaction
name: cAMP activates PKA complex
participant: cAMP
participant: PKA complex

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
type: ProteinReference
xref: Reference/UniProt Q9NQ66
organism: BiologicalState1056

Reference/HMDB HMDB0001498
type: UnificationXref
id: HMDB0001498
db: HMDB

ChemicalStructure/PW C000041
type: ChemicalStructure
structure format: SMILES

Reference/HMDB HMDB0000042
type: UnificationXref
id: HMDB0000042
db: HMDB

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Voltage-dependent L-type calcium channel subunit beta-1
type: ProteinReference
xref: Reference/UniProt Q02641

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: ProteinReference
xref: Reference/UniProt P10644

Reference/KEGG Compound C00076
type: UnificationXref
id: C00076
db: KEGG Compound

ReactionCatalysis203360
type: Catalysis
controller: Serine/threonine-protein phosphatase PP1-beta catalytic subunit
controlled: 3.1.3.16
control type: ACTIVATION

Reference/HMDB HMDB0000058
type: UnificationXref
id: HMDB0000058
db: HMDB

Calcium
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000464
xref: Reference/BioCyc CA+2
xref: Reference/ChemSpider 266
xref: Reference/ChEBI CHEBI 29108
xref: Reference/CAS 14127-61-8
xref: Reference/KEGG Compound C00076
xref: Reference/PubChem-compound 271
chemical formula: Ca
molecular weight: 39.96259
structure: ChemicalStructure/PW C000353

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

Reaction110955
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001155 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/Generic402 Cytosol/Stoichiometry/1.0
right: Inositol 1,4,5-trisphosphate
spontaneous: false
left: Phosphatidylinositol-4,5-bisphosphate
name: Phosphatidylinositol-4,5-bisphosphate → Inositol 1,4,5-trisphosphate
conversion direction: LEFT_TO_RIGHT

Reaction218289
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000029/Stoichiometry/1.0
participant stoichiometry: Compound/PW C001420/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000714/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000065/Stoichiometry/1.0
right: Choline
right: Acetic acid
spontaneous: false
left: Water
left: Acetylcholine
name: Acetylcholine + Water ↔ Acetic acid + Choline
conversion direction: REVERSIBLE

Reaction218288
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001099 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000940 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000065 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000714 Cytosol/Stoichiometry/1.0
right: Acetylcholine
right: Coenzyme A
spontaneous: false
left: Choline
left: Acetyl-CoA
name: Acetyl-CoA + Choline ↔ Acetylcholine + Coenzyme A
conversion direction: REVERSIBLE

Choline O-acetyltransferase
type: Protein
cellular location: Cytosol
entity reference: Choline O-acetyltransferase

cAMP-dependent protein kinase catalytic subunit gamma
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit gamma

Reference/UniProt Q09428
type: UnificationXref
id: Q09428
db: UniProt

TransportCatalysis5738
type: Catalysis
controller: Potassium voltage-gated channel subfamily A member 1
controlled: Transport15217
control type: ACTIVATION

Acetylcholine
type: SmallMolecule
cellular location: Cytosol
entity reference: Acetylcholine

Reference/ChemSpider 5705
type: UnificationXref
id: 5705
db: ChemSpider

Reference/PubChem-compound 444493
type: UnificationXref
id: 444493
db: PubChem-compound

TransportCatalysis5741
type: Catalysis
controller: L-type calcium channel
controlled: Transport15220
control type: ACTIVATION

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit beta

Reference/BioCyc ACETYL-COA
type: UnificationXref
id: ACETYL-COA
db: BioCyc

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Calcium
type: SmallMolecule
entity reference: Calcium

Reference/GENE ONTOLOGY GO 0045202
type: UnificationXref
id: GO:0045202
db: GENE ONTOLOGY

Reference/PubChem-compound 6076
type: UnificationXref
id: 6076
db: PubChem-compound

Potassium
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00238
xref: Reference/HMDB HMDB0000586
xref: Reference/PubChem-compound 813
xref: Reference/ChemSpider 791
xref: Reference/CAS 24203-36-9
xref: Reference/BioCyc K+
xref: Reference/ChEBI CHEBI 29103
chemical formula: K
molecular weight: 38.963707
structure: ChemicalStructure/PW C000457

TransportCatalysis5632
type: Catalysis
controller: L-type calcium channel
controlled: Transport14925
control type: ACTIVATION

Compound/PW C000041/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP
stoichiometric coefficient: 1.0

Protein/1262 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit alpha
stoichiometric coefficient: 1.0

Reference/CAS 62-49-7
type: UnificationXref
id: 62-49-7
db: CAS

ATP-sensitive inward rectifier potassium channel 8
type: ProteinReference
xref: Reference/UniProt Q15842

Phosphatidylinositol-4,5-bisphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Phosphatidylinositol-4,5-bisphosphate

Transport3328
type: Transport
participant stoichiometry: Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353 Cytoplasm/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Cell, Cytoplasm
conversion direction: LEFT_TO_RIGHT

Reference/UniProt P17612
type: UnificationXref
id: P17612
db: UniProt

Inositol 1,4,5-trisphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Inositol 1,4,5-trisphosphate

Calcium_Calmodulin (Homo sapiens)
type: Complex
component stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/1597 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Calcium
component: Calmodulin-1

Compound/PW C000353 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Myosin light chain kinase, smooth muscle
type: ProteinReference
xref: Reference/UniProt Q15746
organism: BiologicalState1056

Compound/PW C000714 Synapse/Stoichiometry/1.0
type: Stoichiometry
physical entity: Acetylcholine
stoichiometric coefficient: 1.0

Acetic acid
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15366
xref: Reference/BioCyc ACET
xref: Reference/CAS 64-19-7
xref: Reference/KEGG Compound C00033
xref: Reference/ChemSpider 171
xref: Reference/HMDB HMDB0000042
xref: Reference/PubChem-compound 176
chemical formula: C2H4O2
molecular weight: 60.02113
structure: ChemicalStructure/PW C000029

Protein/1134/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit gamma
stoichiometric coefficient: 1.0

cAMP-dependent protein kinase catalytic subunit beta
type: ProteinReference
xref: Reference/UniProt P22694

Reaction219325
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/681/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/7708/Stoichiometry/1.0
right: ATP-sensitive inward rectifier potassium channel (IKATP)
spontaneous: false
left: Protein kinase A
name: Protein kinase A → ATP-sensitive inward rectifier potassium channel (IKATP)
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis207644
type: Catalysis
controller: Acetylcholinesterase
controlled: Reaction218289
control type: ACTIVATION

ReactionCatalysis207643
type: Catalysis
controller: Choline O-acetyltransferase
controlled: Reaction218288
control type: ACTIVATION

Reference/HMDB HMDB0000895
type: UnificationXref
id: HMDB0000895
db: HMDB

Reference/CAS 64-19-7
type: UnificationXref
id: 64-19-7
db: CAS

Intermediate conductance calcium-activated potassium channel protein 4
type: Protein
entity reference: Intermediate conductance calcium-activated potassium channel protein 4

Interaction3988
type: MolecularInteraction
interaction type: direct_interaction
name: Protein kinase C activates L-type calcium channel
participant: L-type calcium channel
participant: Protein kinase C alpha type

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Protein/1134 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit gamma
stoichiometric coefficient: 1.0

Reference/GENE ONTOLOGY GO 0070382
type: UnificationXref
id: GO:0070382
db: GENE ONTOLOGY

Acetyl-CoA
type: SmallMolecule
cellular location: Cytosol
entity reference: Acetyl-CoA

Protein/3359/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-beta regulatory subunit
stoichiometric coefficient: 1.0

Serine/threonine-protein phosphatase PP1-beta catalytic subunit
type: Protein
cellular location: Cytosol
entity reference: Serine/threonine-protein phosphatase PP1-beta catalytic subunit

Phosphatidylinositol-4,5-bisphosphate
type: SmallMoleculeReference

Reference/HMDB HMDB0000538
type: UnificationXref
id: HMDB0000538
db: HMDB

ChemicalStructure/PW C000457
type: ChemicalStructure
structure format: SMILES
structure data: [K+]

cAMP
type: SmallMolecule
entity reference: cAMP

Compound/PW C000414/Stoichiometry/1.0
type: Stoichiometry
physical entity: Adenosine triphosphate
stoichiometric coefficient: 1.0

Protein/3358/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-alpha regulatory subunit
stoichiometric coefficient: 1.0

Reference/PubChem-compound 6816
type: UnificationXref
id: 6816
db: PubChem-compound

Amifampridine Action Pathway
type: Pathway
pathway order: PathwayEnzymeReaction/2529307
pathway order: PathwayEnzymeReaction/2529308
pathway order: PathwayEnzymeReaction/2529305
pathway order: PathwayEnzymeReaction/2529306
pathway order: PathwayInteraction/509
pathway order: PathwaySubPathway/120652
pathway order: PathwaySubPathway/120651
pathway order: PathwaySubPathway/120654
pathway order: PathwaySubPathway/120653
pathway order: PathwayTransport/2529312
pathway order: PathwayTransport/2529313
pathway order: PathwayTransport/2529314
pathway order: PathwayTransport/2529315
pathway order: PathwayInteraction/3988
pathway order: PathwayTransport/2529310
pathway order: PathwayInteraction/3920
pathway order: PathwayTransport/2529332
pathway order: PathwayTransport/2529311
pathway order: PathwayEnzymeReaction/2529303
pathway order: PathwayEnzymeReaction/2529304
pathway order: PathwayEnzymeReaction/2529301
pathway order: PathwayEnzymeReaction/2529302
pathway order: PathwayEnzymeReaction/2529300
pathway order: PathwayTransport/2529316
pathway order: PathwayTransport/2529317
pathway order: PathwayInteraction/3918
pathway order: PathwayInteraction/2849
pathway order: PathwayInteraction/4749
pathway order: PathwayTransport/2529343
pathway order: PathwayTransport/2532089
pathway order: PathwayTransport/2532088
pathway order: PathwayEnzymeReaction/2529299
pathway order: PathwayInteraction/3231
pathway order: PathwayInteraction/4
pathway order: PathwayEnzymeReaction/2529297
pathway order: PathwayEnzymeReaction/2529298
pathway component: TransportCatalysis4975
pathway component: TransportCatalysis4974
pathway component: ReactionCatalysis202856
pathway component: TransportCatalysis5741
pathway component: Reaction219325
pathway component: Transport14925
pathway component: Transport14926
pathway component: ReactionCatalysis207644
pathway component: 2.7.11.18
pathway component: ReactionCatalysis207643
pathway component: Transport15217
pathway component: ReactionCatalysis203360
pathway component: Transport13992
pathway component: Transport13991
pathway component: Transport14002
pathway component: Transport13993
pathway component: Interaction3920
pathway component: Interaction3988
pathway component: Transport15593
pathway component: Interaction4
pathway component: Transport15592
pathway component: SubPathwayControl120653
pathway component: SubPathwayControl120654
pathway component: Reaction213775
pathway component: 3.1.3.16
pathway component: Reaction110955
pathway component: TransportCatalysis4984
pathway component: TransportCatalysis5632
pathway component: Reaction219314
pathway component: Reaction219313
pathway component: Reaction218289
pathway component: Reaction219312
pathway component: Muscle Relaxation
pathway component: Reaction218288
pathway component: Transport3328
pathway component: Transport14933
pathway component: Interaction3231
pathway component: Muscle Contraction
pathway component: TransportCatalysis5738
pathway component: Interaction4749
pathway component: Interaction2849
pathway component: Interaction509
pathway component: Transport15220

Amifampridine
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Amifampridine

cAMP
type: SmallMoleculeReference
xref: Reference/PubChem-compound 6076
xref: Reference/HMDB HMDB0000058
xref: Reference/ChEBI CHEBI 17489
xref: Reference/CAS 60-92-4
xref: Reference/BioCyc CAMP
xref: Reference/ChemSpider 5851
xref: Reference/KEGG Compound C00575
chemical formula: C10H12N5O6P
molecular weight: 329.05252
structure: ChemicalStructure/PW C000041

Reference/GENE ONTOLOGY GO 0016529
type: UnificationXref
id: GO:0016529
db: GENE ONTOLOGY

TransportCatalysis4975
type: Catalysis
controller: High affinity choline transporter 1
controlled: Transport13993
control type: ACTIVATION

TransportCatalysis4974
type: Catalysis
controller: Vesicular acetylcholine transporter
controlled: Transport13991
control type: ACTIVATION

Protein/3357/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-beta regulatory subunit
stoichiometric coefficient: 1.0

Reference/CELL TYPE ONTOLOGY CL 0000187
type: UnificationXref
id: CL:0000187
db: CELL TYPE ONTOLOGY

Reference/UniProt O60706
type: UnificationXref
id: O60706
db: UniProt

Compound/PW C000457 Cytoplasm/Stoichiometry/1.0
type: Stoichiometry
physical entity: Potassium
stoichiometric coefficient: 1.0

ATP-binding cassette sub-family C member 8
type: ProteinReference
xref: Reference/UniProt Q09428

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Reference/CAS 85166-31-0
type: UnificationXref
id: 85166-31-0
db: CAS

Reference/BioCyc ACETYLCHOLINE
type: UnificationXref
id: ACETYLCHOLINE
db: BioCyc

Protein kinase C alpha type
type: ProteinReference
xref: Reference/UniProt P17252

ChemicalStructure/PW C097908
type: ChemicalStructure
structure format: SMILES

TransportCatalysis4984
type: Catalysis
controller: Inositol 1,4,5-trisphosphate receptor type 1
controlled: Transport14002
control type: ACTIVATION

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type II-alpha regulatory subunit

Compound/PW C097005 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol 4,5-bisphosphate
stoichiometric coefficient: 1.0

Acetic acid
type: SmallMolecule
entity reference: Acetic acid

Acetylcholinesterase
type: Protein
entity reference: Acetylcholinesterase

ProteinComplex/7708 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Protein kinase A
stoichiometric coefficient: 1.0

Reference/ChemSpider 266
type: UnificationXref
id: 266
db: ChemSpider

Vesicular acetylcholine transporter
type: Protein
entity reference: Vesicular acetylcholine transporter

High affinity choline transporter 1
type: Protein
entity reference: High affinity choline transporter 1

Potassium voltage-gated channel subfamily A member 1
type: Complex
component stoichiometry: Protein/24379 Cell Membrane/Stoichiometry/4.0
cellular location: Cell Membrane
component: Potassium voltage-gated channel subfamily A member 1

Calmodulin-1
type: ProteinReference
xref: Reference/UniProt P0DP23
organism: BiologicalState1056

Compound/PW C001155 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate
stoichiometric coefficient: 1.0

Reference/HMDB HMDB0001423
type: UnificationXref
id: HMDB0001423
db: HMDB

Reference/UniProt P13861
type: UnificationXref
id: P13861
db: UniProt

Protein/1315/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit beta
stoichiometric coefficient: 1.0

Reference/GENE ONTOLOGY GO 0005886
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

Muscarinic acetylcholine receptor M1
type: ProteinReference
xref: Reference/UniProt P11229
organism: BiologicalState789

Voltage-dependent calcium channel subunit alpha-2/delta-1
type: Protein
entity reference: Voltage-dependent calcium channel subunit alpha-2/delta-1

Protein/1315 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit beta
stoichiometric coefficient: 1.0

Compound/PW C000940 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Acetyl-CoA
stoichiometric coefficient: 1.0

ATP-binding cassette sub-family C member 9
type: ProteinReference
xref: Reference/UniProt O60706

Protein/3357 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-beta regulatory subunit
stoichiometric coefficient: 1.0

Reference/UniProt P22694
type: UnificationXref
id: P22694
db: UniProt

Reference/HMDB HMDB0000464
type: UnificationXref
id: HMDB0000464
db: HMDB

Interaction4749
type: MolecularInteraction
interaction type: direct_interaction
name: Amifampridine inhibits Potassium voltage-gated channel subfamily A member 1
participant: Amifampridine
participant: Potassium voltage-gated channel subfamily A member 1

Myocyte
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000187

Reference/HMDB HMDB0000586
type: UnificationXref
id: HMDB0000586
db: HMDB

Potassium voltage-gated channel subfamily A member 1
type: ProteinReference
xref: Reference/UniProt Q09470
organism: BiologicalState73

Reference/PubChem-compound 24742074
type: UnificationXref
id: 24742074
db: PubChem-compound

Muscle
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000887

Reference/UniProt P11229
type: UnificationXref
id: P11229
db: UniProt

Reference/BioCyc CAMP
type: UnificationXref
id: CAMP
db: BioCyc

Neuron
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000540

cAMP-dependent protein kinase catalytic subunit gamma
type: ProteinReference
xref: Reference/UniProt P22612

Transport15593
type: Transport
participant stoichiometry: Compound/PW C113474/Stoichiometry/1.0
right: Amifampridine
spontaneous: false
left: Amifampridine
name: Amifampridine (→) Transport: Homo sapiens, Intestine to Homo sapiens, Blood Vessel
conversion direction: LEFT_TO_RIGHT

Reference/HMDB HMDB0001206
type: UnificationXref
id: HMDB0001206
db: HMDB

Reference/PubChem-compound 5957
type: UnificationXref
id: 5957
db: PubChem-compound

ChemicalStructure/PW C000353
type: ChemicalStructure
structure format: SMILES
structure data: [Ca++]

Interaction4
type: MolecularInteraction
interaction type: direct_interaction
name: Inositol 1,4,5-trisphosphate activates Inositol 1,4,5-trisphosphate receptor
participant: Inositol 1,4,5-trisphosphate
participant: Inositol 1,4,5-trisphosphate receptor type 1

Transport15592
type: Transport
participant stoichiometry: Compound/PW C113474 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C113474/Stoichiometry/1.0
right: Amifampridine
spontaneous: false
left: Amifampridine
name: Amifampridine (→) Transport: Homo sapiens, Blood Vessel to Homo sapiens, Neuron, Cell Membrane
conversion direction: LEFT_TO_RIGHT

Reference/UniProt P54289
type: UnificationXref
id: P54289
db: UniProt

Reference/BioCyc CPD-563
type: UnificationXref
id: CPD-563
db: BioCyc

Reference/UniProt Q16572
type: UnificationXref
id: Q16572
db: UniProt

Reaction219314
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/7708 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/216 Cytosol/Stoichiometry/1.0
right: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
spontaneous: false
left: Protein kinase A
name: Protein kinase A → Phospholipase C
conversion direction: LEFT_TO_RIGHT

Membrane depolarization
type: Pathway
name: ActivatingSubPathway

Reference/UniProt P0DP23
type: UnificationXref
id: P0DP23
db: UniProt

Reaction219313
type: BiochemicalReaction
participant stoichiometry: Protein/2126/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/7708/Stoichiometry/1.0
right: Inositol 1,4,5-trisphosphate receptor type 1
spontaneous: false
left: Protein kinase A
name: Protein kinase A → Inositol 1,4,5-trisphosphate receptor
conversion direction: LEFT_TO_RIGHT

Voltage-dependent L-type calcium channel subunit beta-1
type: Protein
entity reference: Voltage-dependent L-type calcium channel subunit beta-1

Reaction219312
type: BiochemicalReaction
participant stoichiometry: ProteinComplex/15522/Stoichiometry/1.0
participant stoichiometry: ProteinComplex/7708/Stoichiometry/1.0
right: Intermediate conductance calcium-activated potassium channel protein 4
spontaneous: false
left: Protein kinase A
name: Protein kinase A → Intermediate conductance calcium-activated potassium channel protein 4
conversion direction: LEFT_TO_RIGHT

Muscle Relaxation
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway120652
pathway component: SubPathwayInteraction120652
name: SubPathway

G Protien Signalling Cascade
type: Pathway
name: ActivatingSubPathway

Reference/PathWhiz PW127989
type: UnificationXref
id: PW127989
db: PathWhiz

Muscle Contraction
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway120651
pathway component: SubPathwayInteraction120651
name: SubPathway

cAMP-dependent protein kinase catalytic subunit alpha
type: ProteinReference
xref: Reference/UniProt P17612

Protein kinase C alpha type
type: Protein
cellular location: Cytosol
entity reference: Protein kinase C alpha type

Protein/24379/Stoichiometry/4.0
type: Stoichiometry
physical entity: Potassium voltage-gated channel subfamily A member 1
stoichiometric coefficient: 4.0

Inositol 1,4,5-trisphosphate
type: SmallMolecule
entity reference: Inositol 1,4,5-trisphosphate

Adenosine triphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Adenosine triphosphate

Inositol 1,4,5-trisphosphate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001498
xref: Reference/PubChem-compound 439456
xref: Reference/CAS 85166-31-0
xref: Reference/BioCyc inositol 1,4,5-trisphosphate
xref: Reference/KEGG Compound C01245
xref: Reference/ChEBI CHEBI 16595
xref: Reference/ChemSpider 388562
chemical formula: C6H15O15P3
molecular weight: 419.96237
structure: ChemicalStructure/PW C001155

Reference/GENE ONTOLOGY GO 0005615
type: UnificationXref
id: GO:0005615
db: GENE ONTOLOGY

cAMP-dependent protein kinase type I-alpha regulatory subunit
type: Protein
entity reference: cAMP-dependent protein kinase type I-alpha regulatory subunit

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

Compound/PW C000065 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Choline
stoichiometric coefficient: 1.0

Choline
type: SmallMolecule
entity reference: Choline

Transport15220
type: Transport
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353 Extracellular Space/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Homo sapiens, Neuron, Extracellular Space to Homo sapiens, Neuron, Cytosol
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 135948
type: UnificationXref
id: CHEBI:135948
db: ChEBI

Choline
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 15354
xref: Reference/ChemSpider 299
xref: Reference/BioCyc CPD-563
xref: Reference/PubChem-compound 305
xref: Reference/CAS 62-49-7
xref: Reference/HMDB HMDB0000097
xref: Reference/KEGG Compound C00114
chemical formula: C5H14NO
molecular weight: 104.10754
structure: ChemicalStructure/PW C000065

Compound/PW C097908 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Diacylglycerol
stoichiometric coefficient: 1.0

Reference/UniProt Q14643
type: UnificationXref
id: Q14643
db: UniProt

Inositol 1,4,5-trisphosphate receptor type 1
type: ProteinReference
xref: Reference/UniProt Q14643

Protein/1990 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type I-alpha regulatory subunit
stoichiometric coefficient: 1.0

ProteinComplex/681/Stoichiometry/1.0
type: Stoichiometry
physical entity: ATP-sensitive inward rectifier potassium channel (IKATP)
stoichiometric coefficient: 1.0

Reference/UniProt P08590
type: UnificationXref
id: P08590
db: UniProt

Transport15217
type: Transport
participant stoichiometry: Compound/PW C000457 Extracellular Space/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000457 Cytosol/Stoichiometry/1.0
right: Potassium
spontaneous: false
left: Potassium
name: Potassium (→) Transport: Homo sapiens, Neuron, Cytosol to Homo sapiens, Neuron, Extracellular Space
conversion direction: LEFT_TO_RIGHT

Reference/BRENDA TISSUE ONTOLOGY BTO 0000142
type: UnificationXref
id: BTO:0000142
db: BRENDA TISSUE ONTOLOGY

Reference/ChEBI CHEBI 1146900
type: UnificationXref
id: CHEBI:1146900
db: ChEBI

Reference/UniProt P22303
type: UnificationXref
id: P22303
db: UniProt

Transport14002
type: Transport
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353 Sarcoplasmic Reticulum/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Homo sapiens, Cell, Sarcoplasmic Reticulum to Homo sapiens, Cell, Cytosol
conversion direction: LEFT_TO_RIGHT

Inositol 1,4,5-trisphosphate receptor type 1
type: Complex
component stoichiometry: Protein/2126/Stoichiometry/4.0
component: Inositol 1,4,5-trisphosphate receptor type 1

Calcium
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Calcium

Reference/UniProt Q14654
type: UnificationXref
id: Q14654
db: UniProt

Compound/PW C000353 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Reference/BioCyc CO-A
type: UnificationXref
id: CO-A
db: BioCyc

Reference/CAS 72-89-9
type: UnificationXref
id: 72-89-9
db: CAS

Reference/UniProt Q02641
type: UnificationXref
id: Q02641
db: UniProt

Protein/1262/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase catalytic subunit alpha
stoichiometric coefficient: 1.0

Coenzyme A
type: SmallMoleculeReference
xref: Reference/ChEBI CHEBI 1146900
xref: Reference/HMDB HMDB0001423
xref: Reference/PubChem-compound 6816
xref: Reference/BioCyc CO-A
xref: Reference/ChemSpider 6557
xref: Reference/CAS 85-61-0
xref: Reference/KEGG Compound C00010
chemical formula: C21H36N7O16P3S
molecular weight: 767.11523
structure: ChemicalStructure/PW C001099

Interaction509
type: MolecularInteraction
interaction type: direct_interaction
name: Diacylglycerol activates Protein kinase C
participant: Diacylglycerol
participant: Protein kinase C alpha type

Protein/3358 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: cAMP-dependent protein kinase type II-alpha regulatory subunit
stoichiometric coefficient: 1.0

Protein/2126/Stoichiometry/4.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate receptor type 1
stoichiometric coefficient: 4.0

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

Compound/PW C000714 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Acetylcholine
stoichiometric coefficient: 1.0

Reference/UniProt Q15746
type: UnificationXref
id: Q15746
db: UniProt

Reference/ChEBI CHEBI 15422
type: UnificationXref
id: CHEBI:15422
db: ChEBI

cAMP-dependent protein kinase type II-beta regulatory subunit
type: ProteinReference
xref: Reference/UniProt P31323

cAMP-dependent protein kinase type II-alpha regulatory subunit
type: ProteinReference
xref: Reference/UniProt P13861

Reference/ChemSpider 6557
type: UnificationXref
id: 6557
db: ChemSpider

Homo sapiens
type: BioSource
xref: Reference/TAXONOMY 9606

cAMP-dependent protein kinase type I-beta regulatory subunit
type: ProteinReference
xref: Reference/UniProt P31321

ChemicalStructure/PW C000940
type: ChemicalStructure
structure format: SMILES

Reference/PubChem-compound 439456
type: UnificationXref
id: 439456
db: PubChem-compound

Interaction3920
type: MolecularInteraction
interaction type: direct_interaction
name: Acetylcholine activates Muscarinic acetylcholine receptor M1
participant: Muscarinic acetylcholine receptor M1
participant: Acetylcholine

Exocytic Vesicle
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0070382

ATP-sensitive inward rectifier potassium channel 8
type: Protein
entity reference: ATP-sensitive inward rectifier potassium channel 8

BiologicalState1056
type: BioSource
cell type: Myocyte
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Muscle, Myocyte
tissue: Muscle

Reference/UniProt Q15842
type: UnificationXref
id: Q15842
db: UniProt

Protein/3041 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Myosin light chain 3
stoichiometric coefficient: 1.0

Diacylglycerol
type: SmallMolecule
entity reference: Diacylglycerol

L-type calcium channel
type: Complex
cellular location: Cytosol
component: Voltage-dependent calcium channel subunit alpha-2/delta-1
component: Voltage-dependent L-type calcium channel subunit alpha-1C
component: Voltage-dependent L-type calcium channel subunit beta-1

ATP-sensitive inward rectifier potassium channel 11
type: ProteinReference
xref: Reference/UniProt Q14654

cAMP-dependent protein kinase catalytic subunit beta
type: Protein
entity reference: cAMP-dependent protein kinase catalytic subunit beta

ChemicalStructure/PW C000714
type: ChemicalStructure
structure format: SMILES
structure data: CC(=O)OCC[N+](C)(C)C

Myosin light chain kinase, smooth muscle
type: Protein
cellular location: Cytosol
entity reference: Myosin light chain kinase, smooth muscle

cAMP-dependent protein kinase catalytic subunit alpha
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase catalytic subunit alpha

Acetylcholine
type: SmallMolecule
cellular location: Exocytic Vesicle
entity reference: Acetylcholine

Interaction3918
type: MolecularInteraction
interaction type: direct_interaction
name: Calcium activates Acetylcholine
participant: Acetylcholine
participant: Calcium

Reference/TAXONOMY 9606
type: UnificationXref
id: 9606
db: TAXONOMY

Reference/ChEBI CHEBI 15366
type: UnificationXref
id: CHEBI:15366
db: ChEBI

Voltage-dependent calcium channel subunit alpha-2/delta-1
type: Protein
cellular location: Cytosol
entity reference: Voltage-dependent calcium channel subunit alpha-2/delta-1

Potassium
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Potassium

Reference/ChemSpider 392413
type: UnificationXref
id: 392413
db: ChemSpider

2.7.11.18
type: BiochemicalReaction
participant stoichiometry: Protein/3041 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000414 Cytosol/Stoichiometry/1.0
right: Myosin light chain 3
spontaneous: false
EC number: 2.7.11.18
left: Adenosine triphosphate
left: Myosin light chain 3
name: Adenosine triphosphate + Myosin light chain 3 → Myosin LC-P
conversion direction: LEFT_TO_RIGHT

Reference/BioCyc CA+2
type: UnificationXref
id: CA%2b2
db: BioCyc

Reference/ChemSpider 299
type: UnificationXref
id: 299
db: ChemSpider

Muscarinic acetylcholine receptor M1
type: Protein
cellular location: post-synaptic membrane
entity reference: Muscarinic acetylcholine receptor M1

Reference/ChemSpider 171
type: UnificationXref
id: 171
db: ChemSpider

PKA complex
type: Complex
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit gamma
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase type I-beta regulatory subunit
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit beta

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Cell Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005886

Reference/UniProt P31321
type: UnificationXref
id: P31321
db: UniProt

Reference/UniProt P10644
type: UnificationXref
id: P10644
db: UniProt

Reference/UniProt P31323
type: UnificationXref
id: P31323
db: UniProt

Choline
type: SmallMolecule
cellular location: Synapse
entity reference: Choline

Reference/SMPDB SMP0126393
type: UnificationXref
id: SMP0126393
db: SMPDB

ChemicalStructure/PW C000414
type: ChemicalStructure
structure format: SMILES

Reference/PubChem-compound 271
type: UnificationXref
id: 271
db: PubChem-compound

Protein kinase A
type: Complex
component stoichiometry: Protein/1990/Stoichiometry/1.0
component stoichiometry: Protein/1262/Stoichiometry/1.0
component stoichiometry: Protein/3358/Stoichiometry/1.0
component stoichiometry: Protein/1134/Stoichiometry/1.0
component stoichiometry: Protein/3357/Stoichiometry/1.0
component stoichiometry: Protein/1315/Stoichiometry/1.0
component stoichiometry: Protein/3359/Stoichiometry/1.0
component: cAMP-dependent protein kinase catalytic subunit alpha
component: cAMP-dependent protein kinase type I-alpha regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit gamma
component: cAMP-dependent protein kinase type II-alpha regulatory subunit
component: cAMP-dependent protein kinase type I-beta regulatory subunit
component: cAMP-dependent protein kinase type II-beta regulatory subunit
component: cAMP-dependent protein kinase catalytic subunit beta

Compound/PW C001420/Stoichiometry/1.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 1.0

Reference/UniProt P22612
type: UnificationXref
id: P22612
db: UniProt

Interaction2849
type: MolecularInteraction
interaction type: direct_interaction
name: Calcium + Calmodulin (Homo sapiens) activates Myosin light chain kinase, smooth muscle
participant: Myosin light chain kinase, smooth muscle
participant: Calcium_Calmodulin (Homo sapiens)

Myosin light chain 3
type: Protein
cellular location: Cytosol
entity reference: Myosin light chain 3
name: SubPathwayInput

ATP-binding cassette sub-family C member 8
type: Protein
entity reference: ATP-binding cassette sub-family C member 8

Reaction115846
type: BiochemicalReaction
participant stoichiometry: Compound/PW C097005 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C097908 Cytosol/Stoichiometry/1.0
right: Diacylglycerol
spontaneous: false
left: Phosphatidylinositol 4,5-bisphosphate
name: Phosphatidylinositol 4,5-bisphosphate → Diacylglycerol
conversion direction: LEFT_TO_RIGHT

cAMP-dependent protein kinase type I-beta regulatory subunit
type: Protein
cellular location: Cytosol
entity reference: cAMP-dependent protein kinase type I-beta regulatory subunit

Protein kinase C alpha type
type: Protein
entity reference: Protein kinase C alpha type

Reference/PubChem-compound 187
type: UnificationXref
id: 187
db: PubChem-compound

Myosin light chain 3
type: ProteinReference
xref: Reference/UniProt P08590
organism: BiologicalState1056

Compound/PW C001099 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Coenzyme A
stoichiometric coefficient: 1.0

ChemicalStructure/PW C113474
type: ChemicalStructure
structure format: SMILES
structure data: NC1=CNC=CC1=N

Protein/24379 Cell Membrane/Stoichiometry/4.0
type: Stoichiometry
physical entity: Potassium voltage-gated channel subfamily A member 1
stoichiometric coefficient: 4.0

SubPathwayControl120653
type: Interaction
name: SubPathway120653Control
name: ActivatingSubPathwayControl
participant: G Protien Signalling Cascade

SubPathwayControl120654
type: Interaction
name: ActivatingSubPathwayControl
name: SubPathway120654Control
participant: Membrane depolarization
participant: Potassium

Reference/ChEBI CHEBI 15355
type: UnificationXref
id: CHEBI:15355
db: ChEBI

Reference/ChEBI CHEBI 15354
type: UnificationXref
id: CHEBI:15354
db: ChEBI

Reference/ChEBI CHEBI 15351
type: UnificationXref
id: CHEBI:15351
db: ChEBI

Reference/KEGG Compound C01996
type: UnificationXref
id: C01996
db: KEGG Compound

Reference/PubChem-compound 176
type: UnificationXref
id: 176
db: PubChem-compound

Voltage-dependent L-type calcium channel subunit alpha-1C
type: Protein
entity reference: Voltage-dependent L-type calcium channel subunit alpha-1C

Calcium
type: SmallMolecule
cellular location: Sarcoplasmic Reticulum
entity reference: Calcium

Reference/CAS 85-61-0
type: UnificationXref
id: 85-61-0
db: CAS

Reference/ChemSpider 182
type: UnificationXref
id: 182
db: ChemSpider

Synapse
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0045202