Myosin light chain kinase, smooth muscle
type: ProteinReference
xref: Reference/UniProt Q15746
organism: BiologicalState1056

Reference/ChemSpider 17215925
type: UnificationXref
id: 17215925
db: ChemSpider

Compound/PW C000146 Cytosol/Stoichiometry/3.0
type: Stoichiometry
physical entity: NADPH
stoichiometric coefficient: 3.0

Reference/ChemSpider 127983
type: UnificationXref
id: 127983
db: ChemSpider

Reference/ChemSpider 937
type: UnificationXref
id: 937
db: ChemSpider

ChemicalStructure/PW C000693
type: ChemicalStructure
structure format: SMILES
structure data: NCCC1=CNC=N1

direct_interaction
type: InteractionVocabulary

ChemicalStructure/PW C001420
type: ChemicalStructure
structure format: SMILES
structure data: O

Compound/PW C000723 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Citrulline
stoichiometric coefficient: 2.0

L-Arginine
type: SmallMoleculeReference
xref: Reference/BioCyc ARG
xref: Reference/ChEBI CHEBI 16467
xref: Reference/CAS 74-79-3
xref: Reference/KEGG Compound C00062
xref: Reference/ChemSpider 6082
xref: Reference/PubChem-compound 28782
xref: Reference/HMDB HMDB0000517
chemical formula: C6H14N4O2
molecular weight: 174.11168
structure: ChemicalStructure/PW C000396

Serine/threonine-protein phosphatase PP1-beta catalytic subunit
type: Protein
cellular location: Cytosol
entity reference: Serine/threonine-protein phosphatase PP1-beta catalytic subunit

Reference/KEGG Compound C00327
type: UnificationXref
id: C00327
db: KEGG Compound

Reference/PubChem-compound 6992098
type: UnificationXref
id: 6992098
db: PubChem-compound

Reference/PathWhiz PW128019
type: UnificationXref
id: PW128019
db: PathWhiz

Calcium
type: SmallMolecule
cellular location: Cytosol
entity reference: Calcium

ChemicalStructure/PW C097005
type: ChemicalStructure
structure format: SMILES

Reference/HMDB HMDB0003378
type: UnificationXref
id: HMDB0003378
db: HMDB

Voltage-dependent calcium channel subunit alpha-2/delta-2
type: Protein
cellular location: Cell Membrane
entity reference: Voltage-dependent calcium channel subunit alpha-2/delta-2

Histamine H1 receptor
type: Protein
cellular location: Cell Membrane
entity reference: Histamine H1 receptor

Compound/PW C000353/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Compound/PW C001867 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Nitric oxide
stoichiometric coefficient: 2.0

Reference/UniProt P29474
type: UnificationXref
id: P29474
db: UniProt

Reference/CAS 53-59-8
type: UnificationXref
id: 53-59-8
db: CAS

L type Calcium channel
type: Complex
component stoichiometry: Protein/2720/Stoichiometry/1.0
component stoichiometry: Protein/2706/Stoichiometry/2.0
component stoichiometry: Protein/2712/Stoichiometry/1.0
component: Voltage-dependent calcium channel subunit alpha-2/delta-2
component: Voltage-dependent L-type calcium channel subunit beta-1
component: Voltage-dependent P/Q-type calcium channel subunit alpha-1A

Reference/CELL TYPE ONTOLOGY CL 0000187
type: UnificationXref
id: CL:0000187
db: CELL TYPE ONTOLOGY

Reference/PubChem-compound 1038
type: UnificationXref
id: 1038
db: PubChem-compound

Reference/ChEBI CHEBI 16595
type: UnificationXref
id: CHEBI:16595
db: ChEBI

Reference/ChEBI CHEBI 16474
type: UnificationXref
id: CHEBI:16474
db: ChEBI

Reference/ChemSpider 952
type: UnificationXref
id: 952
db: ChemSpider

Interaction3528
type: MolecularInteraction
interaction type: direct_interaction
name: Gq protein signaling cascade activates Phospholipase C
participant: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
participant: Guanine nucleotide-binding protein G(q) subunit alpha

Reference/GENE ONTOLOGY GO 0005829
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Reference/UniProt Q9NQ66
type: UnificationXref
id: Q9NQ66
db: UniProt

ReactionCatalysis208032
type: Catalysis
controller: Nitric-oxide synthase, endothelial
controlled: 1.14.13.39
control type: ACTIVATION

Interaction4736
type: MolecularInteraction
interaction type: direct_interaction
name: Nitric oxide activates Myosin light chain phosphatase
participant: Serine/threonine-protein phosphatase PP1-beta catalytic subunit
participant: Nitric oxide

Protein/2720 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Voltage-dependent L-type calcium channel subunit beta-1
stoichiometric coefficient: 1.0

Reference/BRENDA TISSUE ONTOLOGY BTO 0000887
type: UnificationXref
id: BTO:0000887
db: BRENDA TISSUE ONTOLOGY

Reference/CAS 85166-31-0
type: UnificationXref
id: 85166-31-0
db: CAS

Compound/Generic369 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Diacylglycerol
stoichiometric coefficient: 1.0

Reference/BioCyc ARG
type: UnificationXref
id: ARG
db: BioCyc

Protein kinase C alpha type
type: ProteinReference
xref: Reference/UniProt P17252
organism: BiologicalState1050

Interaction242
type: MolecularInteraction
interaction type: direct_interaction
name: Inositol 1,4,5-trisphosphate activates Inositol 1,4,5-trisphosphate receptor type 1
participant: Inositol 1,4,5-trisphosphate receptor type 1
participant: Inositol 1,4,5-trisphosphate

Reference/BioCyc L-CITRULLINE
type: UnificationXref
id: L-CITRULLINE
db: BioCyc

Reference/ChEBI CHEBI 16480
type: UnificationXref
id: CHEBI:16480
db: ChEBI

Inositol 1,4,5-trisphosphate receptor type 1
type: Protein
entity reference: Inositol 1,4,5-trisphosphate receptor type 1

Compound/PW C097005 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Phosphatidylinositol 4,5-bisphosphate
stoichiometric coefficient: 1.0

Protein/1597 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calmodulin-1
stoichiometric coefficient: 1.0

Interaction3990
type: MolecularInteraction
interaction type: direct_interaction
name: Protein kinase C activates L type Calcium channel
participant: L type Calcium channel
participant: Protein kinase C alpha type

Reference/ChemSpider 1010
type: UnificationXref
id: 1010
db: ChemSpider

Protein/2712/Stoichiometry/1.0
type: Stoichiometry
physical entity: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
stoichiometric coefficient: 1.0

Interaction4728
type: MolecularInteraction
interaction type: direct_interaction
name: Histamine H1 receptor activates Gq protein signaling cascade
participant: Histamine H1 receptor
participant: Guanine nucleotide-binding protein G(q) subunit alpha

Reference/UniProt P62140
type: UnificationXref
id: P62140
db: UniProt

Interaction4729
type: MolecularInteraction
interaction type: direct_interaction
name: Clemastine inhibits Histamine H1 receptor
participant: Histamine H1 receptor
participant: Clemastine

Reference/ChemSpider 266
type: UnificationXref
id: 266
db: ChemSpider

ReactionCatalysis207193
type: Catalysis
controller: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
controlled: 3.1.4.11
control type: ACTIVATION

NADPH
type: SmallMoleculeReference
xref: Reference/CAS 53-57-6
xref: Reference/HMDB HMDB0000221
xref: Reference/PubChem-compound 22833512
xref: Reference/BioCyc NADPH
xref: Reference/ChemSpider 17215925
xref: Reference/KEGG Compound C00005
xref: Reference/ChEBI CHEBI 16474
chemical formula: C21H30N7O17P3
molecular weight: 745.0911
structure: ChemicalStructure/PW C000146

NADP
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00006
xref: Reference/CAS 53-59-8
xref: Reference/BioCyc NAD(P)
xref: Reference/HMDB HMDB0000217
xref: Reference/ChEBI CHEBI 18009
xref: Reference/PubChem-compound 5886
xref: Reference/ChemSpider 5675
chemical formula: C21H28N7O17P3
molecular weight: 743.07544
structure: ChemicalStructure/PW C000143

Reference/UniProt P17252
type: UnificationXref
id: P17252
db: UniProt

Voltage-dependent L-type calcium channel subunit beta-1
type: Protein
cellular location: Cell Membrane
entity reference: Voltage-dependent L-type calcium channel subunit beta-1

Reference/BioCyc NADPH
type: UnificationXref
id: NADPH
db: BioCyc

Protein/2706 Cell Membrane/Stoichiometry/2.0
type: Stoichiometry
physical entity: Voltage-dependent calcium channel subunit alpha-2/delta-2
stoichiometric coefficient: 2.0

Voltage-dependent P/Q-type calcium channel subunit alpha-1A
type: Protein
cellular location: Cell Membrane
entity reference: Voltage-dependent P/Q-type calcium channel subunit alpha-1A

Cytosol
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005829

Clemastine
type: SmallMolecule
entity reference: Clemastine

Calmodulin-1
type: ProteinReference
xref: Reference/UniProt P0DP23
organism: BiologicalState1134

Compound/PW C001155 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate
stoichiometric coefficient: 1.0

Reference/BioCyc CPD-6641
type: UnificationXref
id: CPD-6641
db: BioCyc

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
type: Protein
cellular location: Cytosol
entity reference: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1

Reference/HMDB HMDB0000217
type: UnificationXref
id: HMDB0000217
db: HMDB

Reference/GENE ONTOLOGY GO 0005886
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

L-Arginine
type: SmallMolecule
cellular location: Cytosol
entity reference: L-Arginine

Reference/BioCyc inositol 1,4,5-trisphosphate
type: UnificationXref
id: inositol_1,4,5-trisphosphate
db: BioCyc

Compound/PW C000396 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: L-Arginine
stoichiometric coefficient: 2.0

Reference/PubChem-compound 26987
type: UnificationXref
id: 26987
db: PubChem-compound

Voltage-dependent calcium channel subunit alpha-2/delta-2
type: Protein
entity reference: Voltage-dependent calcium channel subunit alpha-2/delta-2

Reference/KEGG Compound C00007
type: UnificationXref
id: C00007
db: KEGG Compound

Reference/KEGG Compound C00006
type: UnificationXref
id: C00006
db: KEGG Compound

Hydrogen Ion
type: SmallMolecule
cellular location: Cytosol
entity reference: Hydrogen Ion

Reference/ChEBI CHEBI 18009
type: UnificationXref
id: CHEBI:18009
db: ChEBI

Reference/KEGG Compound C00001
type: UnificationXref
id: C00001
db: KEGG Compound

Reference/KEGG Compound C00005
type: UnificationXref
id: C00005
db: KEGG Compound

NADPH
type: SmallMolecule
cellular location: Cytosol
entity reference: NADPH

Reference/UniProt Q9NY47
type: UnificationXref
id: Q9NY47
db: UniProt

Reference/ChemSpider 388562
type: UnificationXref
id: 388562
db: ChemSpider

Reference/HMDB HMDB0000464
type: UnificationXref
id: HMDB0000464
db: HMDB

ChemicalStructure/PW C000143
type: ChemicalStructure
structure format: SMILES

SubPathwayControl120707
type: Interaction
name: ActivatingSubPathwayControl
name: SubPathway120707Control
participant: Muscle relaxation
participant: Myosin light chain 3

Reference/HMDB HMDB0000221
type: UnificationXref
id: HMDB0000221
db: HMDB

Transport15248
type: Transport
participant stoichiometry: Compound/PW C008819 Cell Membrane/Stoichiometry/1.0
participant stoichiometry: Compound/PW C008819/Stoichiometry/1.0
right: Clemastine
spontaneous: true
left: Clemastine
name: Clemastine (→) Transport: Homo sapiens, Intestine to Homo sapiens, Blood Vessel, Endothelial cell, Cell Membrane
conversion direction: REVERSIBLE

Myocyte
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000187

SubPathwayInteraction120705
type: Interaction
name: SubPathway120705Reaction
name: SubPathwayReaction
participant: Myosin light chain 3

SubPathwayInteraction120704
type: Interaction
name: SubPathwayReaction
name: SubPathway120704Reaction
participant: Histamine

ChemicalStructure/PW C000146
type: ChemicalStructure
structure format: SMILES

Reference/UniProt P50148
type: UnificationXref
id: P50148
db: UniProt

Reference/PubChem-compound 24742074
type: UnificationXref
id: 24742074
db: PubChem-compound

Compound/PW C001420 Cytosol/Stoichiometry/4.0
type: Stoichiometry
physical entity: Water
stoichiometric coefficient: 4.0

Muscle
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000887

Reference/CAS 53-57-6
type: UnificationXref
id: 53-57-6
db: CAS

Reference/SMPDB SMP0126421
type: UnificationXref
id: SMP0126421
db: SMPDB

Compound/PW C040034 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Hydrogen Ion
stoichiometric coefficient: 1.0

Citrulline
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00327
xref: Reference/CAS 372-75-8
xref: Reference/ChEBI CHEBI 16349
xref: Reference/PubChem-compound 6992098
xref: Reference/BioCyc L-CITRULLINE
xref: Reference/HMDB HMDB0000904
xref: Reference/ChemSpider 9367
chemical formula: C6H13N3O3
molecular weight: 175.09569
structure: ChemicalStructure/PW C000723

Reference/CAS 372-75-8
type: UnificationXref
id: 372-75-8
db: CAS

Reference/KEGG Compound C06913
type: UnificationXref
id: C06913
db: KEGG Compound

Voltage-dependent P/Q-type calcium channel subunit alpha-1A
type: Protein
entity reference: Voltage-dependent P/Q-type calcium channel subunit alpha-1A

Reference/HMDB HMDB0000904
type: UnificationXref
id: HMDB0000904
db: HMDB

Serine/threonine-protein phosphatase PP1-beta catalytic subunit
type: ProteinReference
xref: Reference/UniProt P62140
organism: BiologicalState1056

Blood Vessel
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0001102

Phosphatidylinositol 4,5-bisphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Phosphatidylinositol 4,5-bisphosphate

Reference/CAS 10102-43-9
type: UnificationXref
id: 10102-43-9
db: CAS

Reference/BioCyc NAD(P)
type: UnificationXref
id: NAD(P)
db: BioCyc

Reference/ChemSpider 6082
type: UnificationXref
id: 6082
db: ChemSpider

Reference/CAS 14127-61-8
type: UnificationXref
id: 14127-61-8
db: CAS

ChemicalStructure/PW C000353
type: ChemicalStructure
structure format: SMILES
structure data: [Ca++]

BiologicalState1134
type: BioSource
cell type: Endothelial cell
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Blood Vessel, Endothelial cell
tissue: Blood Vessel

Hydrogen Ion
type: SmallMoleculeReference
xref: Reference/KEGG Compound C00080
xref: Reference/ChEBI CHEBI 15378
xref: Reference/HMDB HMDB0059597
xref: Reference/PubChem-compound 1038
xref: Reference/ChemSpider 1010
chemical formula: H
molecular weight: 1.007825
structure: ChemicalStructure/PW C040034

Histamine H1 receptor
type: ProteinReference
xref: Reference/UniProt P35367
organism: BiologicalState1134

Reference/UniProt P0DP23
type: UnificationXref
id: P0DP23
db: UniProt

Voltage-dependent L-type calcium channel subunit beta-1
type: Protein
entity reference: Voltage-dependent L-type calcium channel subunit beta-1

Phosphatidylinositol 4,5-bisphosphate
type: SmallMoleculeReference
xref: Reference/PubChem-compound 24742074
chemical formula: C47H80O19P3
molecular weight: 1041.4507
structure: ChemicalStructure/PW C097005

Calmodulin-1
type: Protein
cellular location: Cytosol
entity reference: Calmodulin-1

Reference/KEGG Compound C00388
type: UnificationXref
id: C00388
db: KEGG Compound

Transport15225
type: Transport
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Homo sapiens, Blood Vessel to Homo sapiens, Blood Vessel, Endothelial cell, Cytosol
conversion direction: LEFT_TO_RIGHT

Transport15226
type: Transport
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000353 Endoplasmic Reticulum Lumen/Stoichiometry/1.0
right: Calcium
spontaneous: false
left: Calcium
name: Calcium (→) Transport: Homo sapiens, Blood Vessel, Endothelial cell, Endoplasmic Reticulum Lumen to Homo sapiens, Blood Vessel, Endothelial cell, Cytosol
conversion direction: LEFT_TO_RIGHT

Inositol 1,4,5-trisphosphate
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001498
xref: Reference/PubChem-compound 439456
xref: Reference/CAS 85166-31-0
xref: Reference/BioCyc inositol 1,4,5-trisphosphate
xref: Reference/KEGG Compound C01245
xref: Reference/ChEBI CHEBI 16595
xref: Reference/ChemSpider 388562
chemical formula: C6H15O15P3
molecular weight: 419.96237
structure: ChemicalStructure/PW C001155

Reference/ChEBI CHEBI 29108
type: UnificationXref
id: CHEBI:29108
db: ChEBI

Reference/GENE ONTOLOGY GO 0005737
type: UnificationXref
id: GO:0005737
db: GENE ONTOLOGY

NADP
type: SmallMolecule
cellular location: Cytosol
entity reference: NADP

Reference/UniProt Q14643
type: UnificationXref
id: Q14643
db: UniProt

Inositol 1,4,5-trisphosphate receptor type 1
type: Complex
component stoichiometry: Protein/2126 Endoplasmic Reticulum Membrane/Stoichiometry/4.0
cellular location: Endoplasmic Reticulum Membrane
component: Inositol 1,4,5-trisphosphate receptor type 1

Inositol 1,4,5-trisphosphate receptor type 1
type: ProteinReference
xref: Reference/UniProt Q14643

ChemicalStructure/PW C000723
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CCCNC(N)=O)C(O)=O

Endoplasmic Reticulum Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005789

Reference/CAS 51-45-6
type: UnificationXref
id: 51-45-6
db: CAS

Reference/ChemSpider 9367
type: UnificationXref
id: 9367
db: ChemSpider

Reference/UniProt P08590
type: UnificationXref
id: P08590
db: UniProt

Clemastine H1-Antihistamine Blood Vessel Constriction Action Pathway
type: Pathway
pathway order: PathwaySubPathway/120707
pathway order: PathwaySubPathway/120704
pathway order: PathwaySubPathway/120705
pathway order: PathwayInteraction/509
pathway order: PathwayTransport/2529800
pathway order: PathwayTransport/2529944
pathway order: PathwayTransport/2529801
pathway order: PathwayEnzymeReaction/2529791
pathway order: PathwayInteraction/4729
pathway order: PathwayTransport/2529797
pathway order: PathwayInteraction/4728
pathway order: PathwayInteraction/3528
pathway order: PathwayInteraction/4736
pathway order: PathwayInteraction/4117
pathway order: PathwayInteraction/242
pathway order: PathwayEnzymeReaction/2529795
pathway order: PathwayEnzymeReaction/2529794
pathway order: PathwayInteraction/3990
pathway order: PathwayEnzymeReaction/2529793
pathway order: PathwayInteraction/4132
pathway order: PathwayEnzymeReaction/2529792
pathway component: TransportCatalysis5747
pathway component: TransportCatalysis5746
pathway component: Interaction4117
pathway component: 3.1.4.11
pathway component: Transport14227
pathway component: Interaction4132
pathway component: ReactionCatalysis203360
pathway component: Interaction3528
pathway component: Mast Cells Release Histamine
pathway component: Muscle Contraction
pathway component: ReactionCatalysis208032
pathway component: Interaction4736
pathway component: Reaction213775
pathway component: 1.14.13.39
pathway component: 3.1.3.16
pathway component: Interaction242
pathway component: ReactionCatalysis203359
pathway component: Interaction3990
pathway component: Reaction213774
pathway component: Transport15225
pathway component: Interaction4728
pathway component: SubPathwayControl120707
pathway component: Transport15226
pathway component: Transport15248
pathway component: Interaction509
pathway component: Interaction4729
pathway component: ReactionCatalysis207193
xref: Reference/SMPDB SMP0126421
xref: Reference/PathWhiz PW128019
organism: Homo sapiens

Reference/KEGG Compound C01245
type: UnificationXref
id: C01245
db: KEGG Compound

Protein kinase C alpha type
type: Protein
cellular location: Cell Membrane
entity reference: Protein kinase C alpha type

Reference/CELL TYPE ONTOLOGY CL 0000115
type: UnificationXref
id: CL:0000115
db: CELL TYPE ONTOLOGY

Inositol 1,4,5-trisphosphate receptor type 1
type: Complex
component stoichiometry: Protein/2126/Stoichiometry/4.0
component: Inositol 1,4,5-trisphosphate receptor type 1

Histamine
type: SmallMolecule
entity reference: Histamine
name: SubPathwayOutput

Eye
type: TissueVocabulary
xref: Reference/BRENDA TISSUE ONTOLOGY BTO 0000439

Diacylglycerol
type: SmallMolecule
cellular location: Cytosol
entity reference: Diacylglycerol

BiologicalState1050
type: BioSource
cell type: Myocyte
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Eye, Myocyte
tissue: Eye

Compound/PW C000353 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

Diacylglycerol
type: SmallMoleculeReference

Reference/HMDB HMDB0014428
type: UnificationXref
id: HMDB0014428
db: HMDB

Reference/ChemSpider 5675
type: UnificationXref
id: 5675
db: ChemSpider

Protein/2712 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
stoichiometric coefficient: 1.0

Reference/BioCyc NITRIC-OXIDE
type: UnificationXref
id: NITRIC-OXIDE
db: BioCyc

Guanine nucleotide-binding protein G(q) subunit alpha
type: Protein
cellular location: Cell Membrane
entity reference: Guanine nucleotide-binding protein G(q) subunit alpha

ChemicalStructure/PW C001155
type: ChemicalStructure
structure format: SMILES

Reference/UniProt Q02641
type: UnificationXref
id: Q02641
db: UniProt

Reference/BRENDA TISSUE ONTOLOGY BTO 0001102
type: UnificationXref
id: BTO:0001102
db: BRENDA TISSUE ONTOLOGY

Interaction509
type: MolecularInteraction
interaction type: direct_interaction
name: Diacylglycerol activates Protein kinase C
participant: Protein kinase C alpha type
participant: Diacylglycerol

Protein/2126/Stoichiometry/4.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate receptor type 1
stoichiometric coefficient: 4.0

Cytoplasm
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005737

L type Calcium channel
type: Complex
component stoichiometry: Protein/2706 Cell Membrane/Stoichiometry/2.0
component stoichiometry: Protein/2720 Cell Membrane/Stoichiometry/1.0
component stoichiometry: Protein/2712 Cell Membrane/Stoichiometry/1.0
cellular location: Cell Membrane
component: Voltage-dependent calcium channel subunit alpha-2/delta-2
component: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
component: Voltage-dependent L-type calcium channel subunit beta-1

Reference/UniProt Q15746
type: UnificationXref
id: Q15746
db: UniProt

BoundComplex/506 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium_Calmodulin (Homo sapiens)
stoichiometric coefficient: 1.0

Homo sapiens
type: BioSource
xref: Reference/TAXONOMY 9606

Inositol 1,4,5-trisphosphate
type: SmallMolecule
cellular location: Endoplasmic Reticulum Membrane
entity reference: Inositol 1,4,5-trisphosphate

Compound/PW C008819 Cell Membrane/Stoichiometry/1.0
type: Stoichiometry
physical entity: Clemastine
stoichiometric coefficient: 1.0

Reference/CAS 7732-18-5
type: UnificationXref
id: 7732-18-5
db: CAS

Transport14227
type: Transport
participant stoichiometry: Compound/PW C001867 Cytosol/Stoichiometry/1.0
right: Nitric oxide
spontaneous: true
left: Nitric oxide
name: Nitric oxide (→) Transport: Homo sapiens, Blood Vessel, Endothelial cell, Cytosol to Homo sapiens, Muscle, Myocyte, Cytosol
conversion direction: REVERSIBLE

Reference/ChemSpider 25129
type: UnificationXref
id: 25129
db: ChemSpider

ChemicalStructure/PW C000396
type: ChemicalStructure
structure format: SMILES
structure data: N[C@@H](CCCNC(N)=N)C(O)=O

Protein/872 Cytosol/Stoichiometry/2.0
type: Stoichiometry
physical entity: Nitric oxide synthase, endothelial
stoichiometric coefficient: 2.0

Oxygen
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0001377
xref: Reference/ChemSpider 952
xref: Reference/KEGG Compound C00007
xref: Reference/ChEBI CHEBI 15379
xref: Reference/PubChem-compound 977
xref: Reference/BioCyc CPD-6641
xref: Reference/CAS 7782-44-7
chemical formula: O2
molecular weight: 31.98983
structure: ChemicalStructure/PW C001065

Reference/PubChem-compound 439456
type: UnificationXref
id: 439456
db: PubChem-compound

Reference/KEGG Compound C00062
type: UnificationXref
id: C00062
db: KEGG Compound

Compound/PW C001065 Cytosol/Stoichiometry/4.0
type: Stoichiometry
physical entity: Oxygen
stoichiometric coefficient: 4.0

Reference/CAS 15686-51-8
type: UnificationXref
id: 15686-51-8
db: CAS

Reference/GENE ONTOLOGY GO 0005789
type: UnificationXref
id: GO:0005789
db: GENE ONTOLOGY

Endothelial cell
type: CellVocabulary
xref: Reference/CELL TYPE ONTOLOGY CL 0000115

Reference/PubChem-compound 28782
type: UnificationXref
id: 28782
db: PubChem-compound

Inositol 1,4,5-trisphosphate receptor type 1
type: Protein
cellular location: Endoplasmic Reticulum Membrane
entity reference: Inositol 1,4,5-trisphosphate receptor type 1

BiologicalState1056
type: BioSource
cell type: Myocyte
xref: Reference/TAXONOMY 9606
name: Homo sapiens, Muscle, Myocyte
tissue: Muscle

Reference/GENE ONTOLOGY GO 0005788
type: UnificationXref
id: GO:0005788
db: GENE ONTOLOGY

Voltage-dependent calcium channel subunit alpha-2/delta-2
type: ProteinReference
xref: Reference/UniProt Q9NY47

Compound/PW C008819/Stoichiometry/1.0
type: Stoichiometry
physical entity: Clemastine
stoichiometric coefficient: 1.0

Water
type: SmallMolecule
cellular location: Cytosol
entity reference: Water

Reaction213775
type: BiochemicalReaction
participant stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
participant stoichiometry: BoundComplex/506 Cytosol/Stoichiometry/1.0
participant stoichiometry: Protein/1597 Cytosol/Stoichiometry/1.0
right: Calcium_Calmodulin (Homo sapiens)
spontaneous: false
left: Calcium
left: Calmodulin-1
name: Calcium + Calmodulin → Calcium + Calmodulin (Homo sapiens)
conversion direction: LEFT_TO_RIGHT

3.1.3.16
type: BiochemicalReaction
participant stoichiometry: Protein/3041 Cytosol/Stoichiometry/1.0
right: Myosin light chain 3
spontaneous: false
EC number: 3.1.3.16
left: Myosin light chain 3
name: Myosin LC-P → Myosin light chain 3
conversion direction: LEFT_TO_RIGHT

Reference/PubChem-compound 774
type: UnificationXref
id: 774
db: PubChem-compound

Protein/3041 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Myosin light chain 3
stoichiometric coefficient: 1.0

Voltage-dependent P/Q-type calcium channel subunit alpha-1A
type: ProteinReference
xref: Reference/UniProt O00555

Diacylglycerol
type: SmallMolecule
cellular location: Cytoplasm
entity reference: Diacylglycerol

Guanine nucleotide-binding protein G(q) subunit alpha
type: ProteinReference
xref: Reference/UniProt P50148
organism: BiologicalState1134

Nitric oxide
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0003378
xref: Reference/CAS 10102-43-9
xref: Reference/PubChem-compound 145068
xref: Reference/ChEBI CHEBI 16480
xref: Reference/ChemSpider 127983
xref: Reference/KEGG Compound C00533
xref: Reference/BioCyc NITRIC-OXIDE
chemical formula: NO
molecular weight: 29.997988
structure: ChemicalStructure/PW C001867

Compound/PW C000353 Endoplasmic Reticulum Lumen/Stoichiometry/1.0
type: Stoichiometry
physical entity: Calcium
stoichiometric coefficient: 1.0

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
type: ProteinReference
xref: Reference/UniProt Q9NQ66
organism: BiologicalState1134

Reaction213774
type: BiochemicalReaction
participant stoichiometry: Protein/3041 Cytosol/Stoichiometry/1.0
right: Myosin light chain 3
spontaneous: false
left: Myosin light chain 3
name: Myosin light chain 3 → Myosin LC-P
conversion direction: LEFT_TO_RIGHT

Reference/CAS 7782-44-7
type: UnificationXref
id: 7782-44-7
db: CAS

Clemastine
type: SmallMoleculeReference
xref: Reference/CAS 15686-51-8
xref: Reference/HMDB HMDB0014428
xref: Reference/KEGG Compound C06913
xref: Reference/ChemSpider 25129
xref: Reference/PubChem-compound 26987
xref: Reference/ChEBI CHEBI 3738
chemical formula: C21H26ClNO
molecular weight: 343.1703
structure: ChemicalStructure/PW C008819

Reference/HMDB HMDB0001498
type: UnificationXref
id: HMDB0001498
db: HMDB

Myosin light chain kinase, smooth muscle
type: Protein
cellular location: Cytosol
entity reference: Myosin light chain kinase, smooth muscle

Reference/HMDB HMDB0001377
type: UnificationXref
id: HMDB0001377
db: HMDB

Nitric oxide
type: SmallMolecule
cellular location: Cytosol
entity reference: Nitric oxide

Reference/TAXONOMY 9606
type: UnificationXref
id: 9606
db: TAXONOMY

Reference/PubChem-compound 962
type: UnificationXref
id: 962
db: PubChem-compound

Voltage-dependent L-type calcium channel subunit beta-1
type: ProteinReference
xref: Reference/UniProt Q02641

Guanine nucleotide-binding protein G(q) subunit alpha
type: Protein
cellular location: Cytosol
entity reference: Guanine nucleotide-binding protein G(q) subunit alpha

Endoplasmic Reticulum Lumen
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005788

Reference/PubChem-compound 145068
type: UnificationXref
id: 145068
db: PubChem-compound

Reference/KEGG Compound C00076
type: UnificationXref
id: C00076
db: KEGG Compound

Interaction4117
type: MolecularInteraction
interaction type: direct_interaction
name: Calcium + Calmodulin (Homo sapiens) activates Nitric-oxide synthase, endothelial
participant: Nitric-oxide synthase, endothelial
participant: Calcium_Calmodulin (Homo sapiens)

3.1.4.11
type: BiochemicalReaction
participant stoichiometry: Compound/PW C001155 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C097005 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/Generic369 Cytosol/Stoichiometry/1.0
right: Diacylglycerol
right: Inositol 1,4,5-trisphosphate
spontaneous: false
EC number: 3.1.4.11
left: Phosphatidylinositol 4,5-bisphosphate
name: Phosphatidylinositol 4,5-bisphosphate → Diacylglycerol + Inositol 1,4,5-trisphosphate
conversion direction: LEFT_TO_RIGHT

ReactionCatalysis203360
type: Catalysis
controller: Serine/threonine-protein phosphatase PP1-beta catalytic subunit
controlled: 3.1.3.16
control type: ACTIVATION

Calcium
type: SmallMolecule
cellular location: Endoplasmic Reticulum Lumen
entity reference: Calcium

Reference/KEGG Compound C00080
type: UnificationXref
id: C00080
db: KEGG Compound

Reference/BioCyc CA+2
type: UnificationXref
id: CA%2b2
db: BioCyc

ChemicalStructure/PW C001065
type: ChemicalStructure
structure format: SMILES
structure data: O=O

Protein/2720/Stoichiometry/1.0
type: Stoichiometry
physical entity: Voltage-dependent L-type calcium channel subunit beta-1
stoichiometric coefficient: 1.0

Calcium
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000464
xref: Reference/BioCyc CA+2
xref: Reference/ChemSpider 266
xref: Reference/ChEBI CHEBI 29108
xref: Reference/CAS 14127-61-8
xref: Reference/KEGG Compound C00076
xref: Reference/PubChem-compound 271
chemical formula: Ca
molecular weight: 39.96259
structure: ChemicalStructure/PW C000353

Histamine
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Histamine

Reference/HMDB HMDB0002111
type: UnificationXref
id: HMDB0002111
db: HMDB

1.14.13.39
type: BiochemicalReaction
participant stoichiometry: Compound/PW C040034 Cytosol/Stoichiometry/1.0
participant stoichiometry: Compound/PW C000146 Cytosol/Stoichiometry/3.0
participant stoichiometry: Compound/PW C001065 Cytosol/Stoichiometry/4.0
participant stoichiometry: Compound/PW C000143 Cytosol/Stoichiometry/3.0
participant stoichiometry: Compound/PW C001420 Cytosol/Stoichiometry/4.0
participant stoichiometry: Compound/PW C000723 Cytosol/Stoichiometry/2.0
participant stoichiometry: Compound/PW C000396 Cytosol/Stoichiometry/2.0
participant stoichiometry: Compound/PW C001867 Cytosol/Stoichiometry/2.0
right: NADP
right: Nitric oxide
right: Water
right: Citrulline
spontaneous: false
EC number: 1.14.13.39
left: Oxygen
left: Hydrogen Ion
left: L-Arginine
left: NADPH
name: Hydrogen Ion + 2 L-Arginine + 3 NADPH + 4 Oxygen → 2 Citrulline + 3 NADP + 2 Nitric oxide + 4 Water
conversion direction: LEFT_TO_RIGHT

Reference/ChEBI CHEBI 15377
type: UnificationXref
id: CHEBI:15377
db: ChEBI

Reference/UniProt O00555
type: UnificationXref
id: O00555
db: UniProt

Cell Membrane
type: CellularLocationVocabulary
xref: Reference/GENE ONTOLOGY GO 0005886

Reference/ChEBI CHEBI 15379
type: UnificationXref
id: CHEBI:15379
db: ChEBI

Reference/ChEBI CHEBI 16467
type: UnificationXref
id: CHEBI:16467
db: ChEBI

Reference/ChEBI CHEBI 15378
type: UnificationXref
id: CHEBI:15378
db: ChEBI

ChemicalStructure/PW C040034
type: ChemicalStructure
structure format: SMILES
structure data: [H+]

Reference/ChEBI CHEBI 16349
type: UnificationXref
id: CHEBI:16349
db: ChEBI

Reference/UniProt P35367
type: UnificationXref
id: P35367
db: UniProt

ChemicalStructure/PW C001867
type: ChemicalStructure
structure format: SMILES
structure data: [N]=O

Reference/PubChem-compound 5886
type: UnificationXref
id: 5886
db: PubChem-compound

Reference/BioCyc HISTAMINE
type: UnificationXref
id: HISTAMINE
db: BioCyc

Reference/PubChem-compound 271
type: UnificationXref
id: 271
db: PubChem-compound

ChemicalStructure/PW C008819
type: ChemicalStructure
structure format: SMILES

Oxygen
type: SmallMolecule
cellular location: Cytosol
entity reference: Oxygen

Nitric oxide synthase, endothelial
type: ProteinReference
xref: Reference/UniProt P29474
organism: BiologicalState1134

Myosin light chain 3
type: Protein
cellular location: Cytosol
entity reference: Myosin light chain 3
name: SubPathwayInput
name: SubPathwayActivator

TransportCatalysis5747
type: Catalysis
controller: Inositol 1,4,5-trisphosphate receptor type 1
controlled: Transport15226
control type: ACTIVATION

Protein/2126 Endoplasmic Reticulum Membrane/Stoichiometry/4.0
type: Stoichiometry
physical entity: Inositol 1,4,5-trisphosphate receptor type 1
stoichiometric coefficient: 4.0

TransportCatalysis5746
type: Catalysis
controller: L type Calcium channel
controlled: Transport15225
control type: ACTIVATION

Reference/ChEBI CHEBI 18295
type: UnificationXref
id: CHEBI:18295
db: ChEBI

Reference/KEGG Compound C00533
type: UnificationXref
id: C00533
db: KEGG Compound

Interaction4132
type: MolecularInteraction
interaction type: direct_interaction
name: Histamine activates Histamine H1 receptor
participant: Histamine H1 receptor
participant: Histamine

Reference/PubChem-compound 22833512
type: UnificationXref
id: 22833512
db: PubChem-compound

Reference/HMDB HMDB0000870
type: UnificationXref
id: HMDB0000870
db: HMDB

Reference/ChemSpider 753
type: UnificationXref
id: 753
db: ChemSpider

Muscle relaxation
type: Pathway
name: ActivatingSubPathway

Myosin light chain 3
type: ProteinReference
xref: Reference/UniProt P08590
organism: BiologicalState1056

Mast Cells Release Histamine
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway120704
pathway component: SubPathwayInteraction120704
name: SubPathway

Muscle Contraction
type: Pathway
pathway order: SubPathwayInteractionSteps/SubPathway120705
pathway component: SubPathwayInteraction120705
name: SubPathway

Water
type: SmallMoleculeReference
xref: Reference/PubChem-compound 962
xref: Reference/ChEBI CHEBI 15377
xref: Reference/KEGG Compound C00001
xref: Reference/ChemSpider 937
xref: Reference/HMDB HMDB0002111
xref: Reference/CAS 7732-18-5
chemical formula: H2O
molecular weight: 18.010565
structure: ChemicalStructure/PW C001420

Compound/PW C000143 Cytosol/Stoichiometry/3.0
type: Stoichiometry
physical entity: NADP
stoichiometric coefficient: 3.0

Calcium
type: SmallMolecule
entity reference: Calcium

Protein kinase C alpha type
type: Protein
cellular location: Cytoplasm
entity reference: Protein kinase C alpha type

Clemastine
type: SmallMolecule
cellular location: Cell Membrane
entity reference: Clemastine

Nitric oxide synthase, endothelial
type: Protein
cellular location: Cytosol
entity reference: Nitric oxide synthase, endothelial

Histamine
type: SmallMoleculeReference
xref: Reference/HMDB HMDB0000870
xref: Reference/ChemSpider 753
xref: Reference/PubChem-compound 774
xref: Reference/BioCyc HISTAMINE
xref: Reference/CAS 51-45-6
xref: Reference/ChEBI CHEBI 18295
xref: Reference/KEGG Compound C00388
chemical formula: C5H9N3
molecular weight: 111.07965
structure: ChemicalStructure/PW C000693

Protein/2706/Stoichiometry/2.0
type: Stoichiometry
physical entity: Voltage-dependent calcium channel subunit alpha-2/delta-2
stoichiometric coefficient: 2.0

Nitric-oxide synthase, endothelial
type: Complex
component stoichiometry: Protein/872 Cytosol/Stoichiometry/2.0
cellular location: Cytosol
component: Nitric oxide synthase, endothelial

Reference/PubChem-compound 977
type: UnificationXref
id: 977
db: PubChem-compound

Reference/CAS 74-79-3
type: UnificationXref
id: 74-79-3
db: CAS

Reference/HMDB HMDB0059597
type: UnificationXref
id: HMDB0059597
db: HMDB

ReactionCatalysis203359
type: Catalysis
controller: Myosin light chain kinase, smooth muscle
controlled: Reaction213774
control type: ACTIVATION

Reference/HMDB HMDB0000517
type: UnificationXref
id: HMDB0000517
db: HMDB

Citrulline
type: SmallMolecule
cellular location: Cytosol
entity reference: Citrulline

Compound/PW C001867 Cytosol/Stoichiometry/1.0
type: Stoichiometry
physical entity: Nitric oxide
stoichiometric coefficient: 1.0

Inositol 1,4,5-trisphosphate
type: SmallMolecule
cellular location: Cytosol
entity reference: Inositol 1,4,5-trisphosphate

Calcium_Calmodulin (Homo sapiens)
type: Complex
component stoichiometry: Compound/PW C000353 Cytosol/Stoichiometry/1.0
component stoichiometry: Protein/1597 Cytosol/Stoichiometry/1.0
cellular location: Cytosol
component: Calcium
component: Calmodulin-1

Reference/BRENDA TISSUE ONTOLOGY BTO 0000439
type: UnificationXref
id: BTO:0000439
db: BRENDA TISSUE ONTOLOGY

Reference/ChEBI CHEBI 3738
type: UnificationXref
id: CHEBI:3738
db: ChEBI