Hydrogen Ion + Octanoyl-CoA + an oxidized electron-transfer flavoprotein → (2E)-Octenoyl-CoA + Reduced electron-transfer flavoprotein
FadJ component of anaerobic fatty acid oxidation complex
(2E)-Octenoyl-CoA → 3-hydroxyoctanoyl-CoA + Water
3-hydroxyoctanoyl-CoA + NAD → 3-oxooctanoyl-CoA + Hydrogen Ion + NADH
3-hydroxyoctanoyl-CoA → (2E)-Octenoyl-CoA
3-oxooctanoyl-CoA + Coenzyme A → Acetyl-CoA + Hexanoyl-CoA
Adenosine triphosphate + Caprylic acid + Coenzyme A → Adenosine monophosphate + Octanoyl-CoA
an oxidized electron-transfer flavoprotein