3-Methyl-2-oxovaleric acid + Acetyl-CoA + Water → 2-Isopropylmalic acid + Coenzyme A + Hydrogen Ion
2-Isopropylmalic acid → Isopropylmaleate + Water
Isopropylmaleate + Water → 3-Isopropylmalate
3-Isopropylmalate + NAD → 2-Isopropyl-3-oxosuccinate + Hydrogen Ion + NADH
Ketoleucine + L-Glutamic acid → L-Leucine + Oxoglutaric acid
Anaerobiospirillum succiniciproducens DSM 6400
2-Isopropyl-3-oxosuccinate + Hydrogen Ion → Carbon dioxide + Ketoleucine
branched-chain amino-acid aminotransferase
L-Leucine (→) Transport: Anaerobiospirillum succiniciproducens DSM 6400, Cytosol to Anaerobiospirillum succiniciproducens DSM 6400, Periplasmic Space