DNA-directed RNA polymerase subunit alpha
branched-chain amino-acid aminotransferase
2-Isopropyl-3-oxosuccinate + Hydrogen Ion → Carbon dioxide + Ketoleucine
3-Methyl-2-oxovaleric acid + Acetyl-CoA + Water → 2-Isopropylmalic acid + Coenzyme A + Hydrogen Ion
2-Isopropylmalic acid → Isopropylmaleate + Water
Ketoleucine + L-Glutamic acid → L-Leucine + Oxoglutaric acid
Isopropylmaleate + Water → 3-Isopropylmalate
3-Isopropylmalate + NAD → 2-Isopropyl-3-oxosuccinate + Hydrogen Ion + NADH
L-Leucine (→) Transport: Kluyvera ascorbata ATCC 33433, Cytosol to Kluyvera ascorbata ATCC 33433, Periplasmic Space