Branched-chain-amino-acid aminotransferase
3-isopropylmalate dehydratase small subunit
L-Leucine (→) Transport: Acinetobacter johnsonii SH046, Cytosol to Acinetobacter johnsonii SH046, Periplasmic Space
2-Isopropyl-3-oxosuccinate + Hydrogen Ion → Carbon dioxide + Ketoleucine
branched-chain amino-acid aminotransferase
3-Methyl-2-oxovaleric acid + Acetyl-CoA + Water → 2-Isopropylmalic acid + Coenzyme A + Hydrogen Ion
2-Isopropylmalic acid → Isopropylmaleate + Water
3-Isopropylmalate + NAD → 2-Isopropyl-3-oxosuccinate + Hydrogen Ion + NADH
Isopropylmaleate + Water → 3-Isopropylmalate
Ketoleucine + L-Glutamic acid → L-Leucine + Oxoglutaric acid