3-Methyl-2-oxovaleric acid + Acetyl-CoA + Water → 2-Isopropylmalic acid + Coenzyme A + Hydrogen Ion
Isopropylmaleate + Water → 3-Isopropylmalate
2-Isopropylmalic acid → Isopropylmaleate + Water
L-Leucine (→) Transport: Escherichia coli O103:H2 str. 12009, Cytosol to Escherichia coli O103:H2 str. 12009, Periplasmic Space
Ketoleucine + L-Glutamic acid → L-Leucine + Oxoglutaric acid
3-Isopropylmalate + NAD → 2-Isopropyl-3-oxosuccinate + Hydrogen Ion + NADH
2-Isopropyl-3-oxosuccinate + Hydrogen Ion → Carbon dioxide + Ketoleucine
branched-chain amino-acid aminotransferase