Rolitetracycline is a broad spectrum second antibiotic formed by the N-aminomethylation of the carboxamide function of tetracycline. Classified as a second generation tetracycline due to it’s semi-synthesis, rolitetracycline like tetracycline inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit. This prevents tRNA from interacting with the ribosome ultimately halting the addition of amino acids to peptide chains used in protein synthesis.

Rolitetracycline Pathway References