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Pathway Description
Asparagine Biosynthesis
Escherichia coli
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2015-03-16
Last Updated: 2025-11-10
Asparagine is an amino acid used in protein synthesis, specifically the biosynthesis of glycoproteins. In E.coli, L-asparagine can be synthesized from L-aspartic acid by either utilizing asparagine synthetase B with L-glutamine or ammonia. Both reactions are driven by ATP however the reaction with ammonia utilizes both asparagine synthetase B and aspartate-ammonia ligase.
References
Asparagine Biosynthesis References
Willis RC, Woolfolk CA: L-asparagine uptake in Escherichia coli. J Bacteriol. 1975 Sep;123(3):937-45.
Pubmed: 239925
Scofield MA, Lewis WS, Schuster SM: Nucleotide sequence of Escherichia coli asnB and deduced amino acid sequence of asparagine synthetase B. J Biol Chem. 1990 Aug 5;265(22):12895-902.
Pubmed: 1973930
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.
Pubmed: 8905232
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Nakamura M, Yamada M, Hirota Y, Sugimoto K, Oka A, Takanami M: Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12. Nucleic Acids Res. 1981 Sep 25;9(18):4669-76. doi: 10.1093/nar/9.18.4669.
Pubmed: 6117826
Buhk HJ, Messer W: The replication origin region of Escherichia coli: nucleotide sequence and functional units. Gene. 1983 Oct;24(2-3):265-79. doi: 10.1016/0378-1119(83)90087-2.
Pubmed: 6357950
Burland V, Plunkett G 3rd, Daniels DL, Blattner FR: DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics. 1993 Jun;16(3):551-61. doi: 10.1006/geno.1993.1230.
Pubmed: 7686882
Kuramitsu S, Okuno S, Ogawa T, Ogawa H, Kagamiyama H: Aspartate aminotransferase of Escherichia coli: nucleotide sequence of the aspC gene. J Biochem. 1985 Apr;97(4):1259-62. doi: 10.1093/oxfordjournals.jbchem.a135173.
Pubmed: 3897210
Fotheringham IG, Dacey SA, Taylor PP, Smith TJ, Hunter MG, Finlay ME, Primrose SB, Parker DM, Edwards RM: The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes. Biochem J. 1986 Mar 15;234(3):593-604. doi: 10.1042/bj2340593.
Pubmed: 3521591
Kondo K, Wakabayashi S, Yagi T, Kagamiyama H: The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes. Biochem Biophys Res Commun. 1984 Jul 18;122(1):62-7. doi: 10.1016/0006-291x(84)90439-x.
Pubmed: 6378205
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
Jerlstrom PG, Bezjak DA, Jennings MP, Beacham IR: Structure and expression in Escherichia coli K-12 of the L-asparaginase I-encoding ansA gene and its flanking regions. Gene. 1989 May 15;78(1):37-46. doi: 10.1016/0378-1119(89)90312-0.
Pubmed: 2670682
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. doi: 10.1093/dnares/3.6.363.
Pubmed: 9097039
Jennings MP, Beacham IR: Analysis of the Escherichia coli gene encoding L-asparaginase II, ansB, and its regulation by cyclic AMP receptor and FNR proteins. J Bacteriol. 1990 Mar;172(3):1491-8. doi: 10.1128/jb.172.3.1491-1498.1990.
Pubmed: 2407723
Bonthron DT: L-asparaginase II of Escherichia coli K-12: cloning, mapping and sequencing of the ansB gene. Gene. 1990 Jul 2;91(1):101-5. doi: 10.1016/0378-1119(90)90168-q.
Pubmed: 2144836
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000833
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