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Pathway Description
Secondary Metabolites: Shikimate Pathway
Escherichia coli
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2015-07-16
Last Updated: 2025-10-15
The biosynthesis of shikimate starts with D-Erythrose-4-phosphate getting transformed into 3-deoxy-D-arabino-heptulosonate-7-phosphate through a phospho-2-dehydro-3-deoxyheptonate aldolase. This is followed by a 3-dehydroquinate synthase converting the 3-deoxy-D-arabino-heptulosonate-7-phosphate into a 3-dehydroquinate which in turn is conveted to 3-dehydroshikimate through a 3-dehydroquinate dehydratase. A this point 3-dehydroshikimate can be turned into Shikimic acid through 2 different reactions involving an NADPH driven Quinate/shikimate dehydrogenase or a NADPH driven shikimate dehydrogenase 2.
Shikimate can also be transported through a shikimate:H+ symporter.
References
Secondary Metabolites: Shikimate Pathway References
Escherichia coli and Salmonella: Cellular and Molecular Biology (EcoSal). Online edition.
Garner CC, Herrmann KM: Operator mutations of the Escherichia coli aroF gene. J Biol Chem. 1985 Mar 25;260(6):3820-5.
Pubmed: 2857723
Shultz J, Hermodson MA, Garner CC, Herrmann KM: The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. J Biol Chem. 1984 Aug 10;259(15):9655-61.
Pubmed: 6146618
Hudson GS, Davidson BE: Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12. J Mol Biol. 1984 Dec 25;180(4):1023-51. doi: 10.1016/0022-2836(84)90269-9.
Pubmed: 6396419
Hudson GS, Rellos P, Davidson BE: Two promoters control the aroH gene of Escherichia coli. Gene. 1991 Jun 15;102(1):87-91. doi: 10.1016/0378-1119(91)90544-l.
Pubmed: 1677907
Ray JM, Yanofsky C, Bauerle R: Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. J Bacteriol. 1988 Dec;170(12):5500-6. doi: 10.1128/jb.170.12.5500-5506.1988.
Pubmed: 2903857
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. doi: 10.1093/dnares/3.6.363.
Pubmed: 9097039
Davies WD, Davidson BE: The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12. Nucleic Acids Res. 1982 Jul 10;10(13):4045-58. doi: 10.1093/nar/10.13.4045.
Pubmed: 6125934
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.
Pubmed: 8905232
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Millar G, Coggins JR: The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12. FEBS Lett. 1986 May 5;200(1):11-7. doi: 10.1016/0014-5793(86)80501-4.
Pubmed: 3009224
Lyngstadaas A, Lobner-Olesen A, Boye E: Characterization of three genes in the dam-containing operon of Escherichia coli. Mol Gen Genet. 1995 Jun 10;247(5):546-54. doi: 10.1007/bf00290345.
Pubmed: 7603433
Duncan K, Chaudhuri S, Campbell MS, Coggins JR: The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase. Biochem J. 1986 Sep 1;238(2):475-83. doi: 10.1042/bj2380475.
Pubmed: 3541912
Chaudhuri S, Duncan K, Graham LD, Coggins JR: Identification of the active-site lysine residues of two biosynthetic 3-dehydroquinases. Biochem J. 1991 Apr 1;275 ( Pt 1):1-6. doi: 10.1042/bj2750001.
Pubmed: 1826831
Whipp MJ, Camakaris H, Pittard AJ: Cloning and analysis of the shiA gene, which encodes the shikimate transport system of escherichia coli K-12. Gene. 1998 Mar 16;209(1-2):185-92. doi: 10.1016/s0378-1119(98)00043-2.
Pubmed: 9524262
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T, et al.: A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):379-92. doi: 10.1093/dnares/3.6.379.
Pubmed: 9097040
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
Anton IA, Coggins JR: Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase. Biochem J. 1988 Jan 15;249(2):319-26. doi: 10.1042/bj2490319.
Pubmed: 3277621
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0001001
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