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Pathway Description
Operon: Cytidyl Pyrophosphate Biosynthesis of Isoprenoids
Escherichia coli
Category:
Metabolite Pathway
Sub-Category:
Signaling
Created: 2015-07-17
Last Updated: 2019-08-16
The ispDF operon in E. coli contains two genes that encode proteins that are used to form building blocks for isoprenoid compounds. There is currently no information on the regulation of this operon.
The first gene in the operon, ispD, also known as ygbP, encodes the protein 2-C-methyl-D-erythritol 4-phosphate (MEP) cytidylyltransferase. This protein catalyzes the reaction of MEP, a hydrogen ion and cytidine triphosphate, which form a pyrophosphate molecule and 4-CDP-2-C-methyl-D-erythritol.
The second gene, ispF, also known as ygbB or mecS, encodes for the protein 2-C-D-erythritol 2,4-cyclodiphosphate synthase. This protein is involved in the formation of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are building blocks in the biosynthesis of isoprenoids, also known as terpenoids.
References
Operon: Cytidyl Pyrophosphate Biosynthesis of Isoprenoids References
Rohdich F, Wungsintaweekul J, Fellermeier M, Sagner S, Herz S, Kis K, Eisenreich W, Bacher A, Zenk MH: Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11758-63.
Pubmed: 10518523
Englund E, Shabestary K, Hudson EP, Lindberg P: Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound. Metab Eng. 2018 Sep;49:164-177. doi: 10.1016/j.ymben.2018.07.004. Epub 2018 Jul 17.
Pubmed: 30025762
Richard SB, Ferrer JL, Bowman ME, Lillo AM, Tetzlaff CN, Cane DE, Noel JP: Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway. J Biol Chem. 2002 Mar 8;277(10):8667-72. doi: 10.1074/jbc.C100739200. Epub 2002 Jan 10.
Pubmed: 11786530
Li C, Ichikawa JK, Ravetto JJ, Kuo HC, Fu JC, Clarke S: A new gene involved in stationary-phase survival located at 59 minutes on the Escherichia coli chromosome. J Bacteriol. 1994 Oct;176(19):6015-22. doi: 10.1128/jb.176.19.6015-6022.1994.
Pubmed: 7928962
Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Luttgen H, Sagner S, Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F: Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2486-90. doi: 10.1073/pnas.040554697.
Pubmed: 10694574
Kemp LE, Bond CS, Hunter WN: Structure of a tetragonal crystal form of Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):607-10. doi: 10.1107/s090744490202365x. Epub 2003 Feb 21.
Pubmed: 12595740
Rohdich F, Wungsintaweekul J, Fellermeier M, Sagner S, Herz S, Kis K, Eisenreich W, Bacher A, Zenk MH: Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11758-63. doi: 10.1073/pnas.96.21.11758.
Pubmed: 10518523
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
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