PathBank

Pathway Description

Selenium Metabolism

Escherichia coli

Category:

Metabolite Pathway

Sub-Category:

Metabolic

Created: 2015-09-10

Last Updated: 2025-01-27

The selenium metabolism begins with the introduction of selenate and selenite to the cytosol through a sulphate permease system. Once in the cell, selenate can be reduced to selenite through nitrate reductases A and Z. Selenite then interacts with glutathione and 2 hydrogen ions resulting in the release of 2 water molecules, a hydroxide molecule, a glutathione disulfide and a selenodiglutathione. The latter compound then reacts with NADPH+H resulting in the release of a NADP, a glutathione and a glutathioselenol. Glutathiolselenol can then be oxidize resulting in a a glutathiolselenol ion which can then interact with a water molecule resulting in a release of glutathion and selenium Glutathiolselenol can also react with NADPH and hydrogen ion resulting in a release of glutathione, NADP, a hydroxide molecule and a hydrogen selenide. This compound can react in a reversible reaction by being oxidized resulting in a hydrogen selenide ion . This compound can then be phosphorylated by interacting with an ATP and releasing a AMP, a phosphate and a selenophosphate.

References

Selenium Metabolism References

Turner RJ, Weiner JH, Taylor DE: Selenium metabolism in Escherichia coli. Biometals. 1998 Sep;11(3):223-7.

Pubmed: 9850565

Sirko A, Hryniewicz M, Hulanicka D, Bock A: Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster. J Bacteriol. 1990 Jun;172(6):3351-7. doi: 10.1128/jb.172.6.3351-3357.1990.

Pubmed: 2188958

Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. doi: 10.1093/dnares/4.2.91.

Pubmed: 9205837

Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.

Pubmed: 9278503

Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M: Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon. Mol Gen Genet. 1990 Jun;222(1):104-11. doi: 10.1007/bf00283030.

Pubmed: 2233673

Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V: Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer. Mol Gen Genet. 1989 Aug;218(2):249-56. doi: 10.1007/bf00331275.

Pubmed: 2674654

Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.

Pubmed: 8905232

Sodergren EJ, DeMoss JA: narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes. J Bacteriol. 1988 Apr;170(4):1721-9. doi: 10.1128/jb.170.4.1721-1729.1988.

Pubmed: 2832376

Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. doi: 10.1093/dnares/3.6.363.

Pubmed: 9097039

This pathway was propagated using PathWhiz - Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.

Propagated from SMP0001908

Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.

Visualize Compound Data

		Adenosine monophosphate
		Adenosine triphosphate
		Electron
		Glutathione
		glutathioselenium
		glutathioselenol
		Hydrogen Ion
		Hydrogen selenide
		hydrogen selenide ion
		Hydroxide
		NADP
		NADPH
		Oxidized glutathione
		Phosphate
		Selenic acid
		Selenite
		Selenium
		Selenodiglutathione
		Selenophosphate
		Water

Visualize Protein Data

		cytochrom c550 subunit nitrate reductase
		Nitrate reductase molybdenum cofactor assembly chaperone NarJ
		Periplasmic nitrate reductase
		Probable nitrate reductase molybdenum cofactor assembly chaperone NarW
		Respiratory nitrate reductase 1 alpha chain
		Respiratory nitrate reductase 1 beta chain
		Respiratory nitrate reductase 1 gamma chain
		Respiratory nitrate reductase 2 alpha chain
		Respiratory nitrate reductase 2 beta chain
		Respiratory nitrate reductase 2 gamma chain
		Sulfate transport system permease protein cysT
		Sulfate transport system permease protein cysW
		Sulfate/thiosulfate import ATP-binding protein cysA