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Pathway Description
Operon: Mannose Uptake
Escherichia coli
Category:
Protein Pathway
Sub-Categories:
Gene Regulatory
Transport/Degradation
Cellular Response
Created: 2015-10-28
Last Updated: 2019-12-04
The manXYZ operon in E. coli contains three genes that encode for different components of the sugar phosphotransferase system (PTS system) enzyme II. This enzyme is involved specifcally in the transport of mannose. This operon can be activated by the cAMP-activated global transcriptional regulator CRP (CAP), which binds to a site upstream from the promoter. Because of its location, the promoter of this operon is considered a class II promoter, meaning that the binding site for CAP overlaps the site where RNA polymerase binds.
The first gene in the operon, manX, encodes the PTS system mannose-specific EIIAB component protein with an A and B domain that are linked. The protein is a dimer in the cell, and is found in the inner membrane of the bacteria, as it is a protein that allows mannose to enter the cell.
The second gene, manY, encodes the mannose permease IIC component, which is found in the cell's inner membrane as part of the permease complex. The protein products of manX and manY are required for lambda phage infection of the cell, as they likely form the pore that allows for its DNA to penetrate the cell.
The final gene, manZ, encodes the mannose permease IID component. This protein is found in the cell's inner membrane along with the other two components, and is responsible for forming the transmembrane channel, along with the ManY protein.
References
Operon: Mannose Uptake References
Plumbridge J, Kolb A: CAP and Nag repressor binding to the regulatory regions of the nagE-B and manX genes of Escherichia coli. J Mol Biol. 1991 Feb 20;217(4):661-79.
Pubmed: 1848637
Zheng D, Constantinidou C, Hobman JL, Minchin SD: Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. Nucleic Acids Res. 2004 Nov 1;32(19):5874-93. doi: 10.1093/nar/gkh908. Print 2004.
Pubmed: 15520470
Niu W, Kim Y, Tau G, Heyduk T, Ebright RH: Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase. Cell. 1996 Dec 13;87(6):1123-34. doi: 10.1016/s0092-8674(00)81806-1.
Pubmed: 8978616
Erni B, Zanolari B, Kocher HP: The mannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA. J Biol Chem. 1987 Apr 15;262(11):5238-47.
Pubmed: 2951378
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T, et al.: A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):379-92. doi: 10.1093/dnares/3.6.379.
Pubmed: 9097040
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Cossart P, Gicquel-Sanzey B: Cloning and sequence of the crp gene of Escherichia coli K 12. Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
Pubmed: 6280141
Aiba H, Fujimoto S, Ozaki N: Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 1982 Feb 25;10(4):1345-61. doi: 10.1093/nar/10.4.1345.
Pubmed: 6280140
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