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Pathway Description
Operon: dnaAN-recF
Escherichia coli
Category:
Protein Pathway
Sub-Categories:
Gene Regulatory
Cellular Response
Created: 2015-11-05
Last Updated: 2025-01-24
The dnaAN-recF operon in E. coli contains three genes that encode proteins involved in DNA repair and replication. The operon can be activated by the binding of HTH-type transcriptional regulator ArgP, which can bind upstream or downstream of the promoter, allowing for transcription activation. The operon can also be controlled to the binding of the chromosomal replication indicator protein DnaA complexed with ATP, which can either inhibit or activate transcription of the operon depending on the number of DnaA-ATP complexes bound. When cells have grown, DnaA is produced and can accumulate, leading to more availability in the cell to bind to the operon binding sites. When enough is bound, it unwinds the DNA, allowing helicases to bind, promoting transcription of the operon.
The first gene, dnaA, encodes the chromosomal replication initiator protein DnaA, which acts on this operon, as well as several others involved in DNA replication within the cell.
The second gene, dnaN, encodes the DNA polymerase III subunit beta, also known as the beta sliding clamp. This protein clamps DNA polymerase III onto the DNA, allowing it to act along the length of the DNA.
The final gene in the operon, recF, encodes the DNA replication and repair protein RecF, a protein required for DNA replication, as well as recombination.
References
Operon: dnaAN-recF References
Saggioro C, Olliver A, Sclavi B: Temperature-dependence of the DnaA-DNA interaction and its effect on the autoregulation of dnaA expression. Biochem J. 2013 Jan 15;449(2):333-41. doi: 10.1042/BJ20120876.
Pubmed: 23092251
Perez-Roger I, Garcia-Sogo M, Navarro-Avino JP, Lopez-Acedo C, Macian F, Armengod ME: Positive and negative regulatory elements in the dnaA-dnaN-recF operon of Escherichia coli. Biochimie. 1991 Feb-Mar;73(2-3):329-34.
Pubmed: 1883890
Ohmori H, Kimura M, Nagata T, Sakakibara Y: Structural analysis of the dnaA and dnaN genes of Escherichia coli. Gene. 1984 May;28(2):159-70.
Pubmed: 6234204
Marbaniang CN, Gowrishankar J: Role of ArgP (IciA) in lysine-mediated repression in Escherichia coli. J Bacteriol. 2011 Nov;193(21):5985-96. doi: 10.1128/JB.05869-11. Epub 2011 Sep 2.
Pubmed: 21890697
Macian F, Perez-Roger I, Armengod ME: An improved vector system for constructing transcriptional lacZ fusions: analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli. Gene. 1994 Jul 22;145(1):17-24.
Pubmed: 8045420
Lee YS, Hwang DS: Occlusion of RNA polymerase by oligomerization of DnaA protein over the dnaA promoter of Escherichia coli. J Biol Chem. 1997 Jan 3;272(1):83-8.
Pubmed: 8995231
Lee Y, Lee H, Yim J, Hwang D: The binding of two dimers of IciA protein to the dnaA promoter 1P element enhances the binding of RNA polymerase to the dnaA promoter 1P. Nucleic Acids Res. 1997 Sep 1;25(17):3486-9.
Pubmed: 9254708
Lee YS, Kim H, Hwang DS: Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli. Mol Microbiol. 1996 Jan;19(2):389-96.
Pubmed: 8825783
Hansen FG, Hansen EB, Atlung T: The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli. EMBO J. 1982;1(9):1043-8.
Pubmed: 6329723
Ohmori H, Kimura M, Nagata T, Sakakibara Y: Structural analysis of the dnaA and dnaN genes of Escherichia coli. Gene. 1984 May;28(2):159-70. doi: 10.1016/0378-1119(84)90253-1.
Pubmed: 6234204
Georgopoulos C: The E. coli dnaA initiation protein: a protein for all seasons. Trends Genet. 1989 Oct;5(10):319-21.
Pubmed: 2558436
Armengod ME, Garcia-Sogo M, Lambies E: Transcriptional organization of the dnaN and recF genes of Escherichia coli K-12. J Biol Chem. 1988 Aug 25;263(24):12109-14.
Pubmed: 2841344
Armengod ME, Lambies E: Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences. Gene. 1986;43(3):183-96. doi: 10.1016/0378-1119(86)90206-4.
Pubmed: 3527871
Thony B, Hwang DS, Fradkin L, Kornberg A: iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4066-70. doi: 10.1073/pnas.88.10.4066.
Pubmed: 2034653
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
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