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Pathway Description
Operon: Pyrimidine Biosynthesis Inactivation
Escherichia coli
Category:
Metabolite Pathway
Sub-Category:
Signaling
Created: 2015-11-10
Last Updated: 2024-08-05
The pyrimidine biosynthesis operon consists of the genes pyrB and pyrI. This is a bicistronic operon. The operon transcription is negatively regulated by the intracellular levels of UTP. The higher the UTP levels, the less expression of the operon. High concentrations of UTP indicate sufficient pyrimidine levels hence UTP binds to the regulatory subunit of ATCase ( catalyzes the first committed step in pyrimidine biosynthesis), resulting in a conformational change and reduced activity. High UTP levels also repress the transcription of this operon through the pyrl regulatory protein, which binds to the promoter region of the pyr operon, preventing RNA polymerase from binding and initiating transcription.
References
Operon: Pyrimidine Biosynthesis Inactivation References
Turnbough CL Jr, Hicks KL, Donahue JP: Attenuation control of pyrBI operon expression in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1983 Jan;80(2):368-72.
Pubmed: 6300835
Wild, J.R., Loughrey-Chen, S.J. and Corder, T.S., 1989. In the presence of CTP, UTP becomes an allosteric inhibitor of aspartate transcarbamoylase. Proceedings of the National Academy of Sciences, 86(1), pp.46-50.
Turnbough Jr, C.L., Hicks, K.L. and Donahue, J.P., 1983. Attenuation control of pyrBI operon expression in Escherichia coli K-12. Proceedings of the National Academy of Sciences, 80(2), pp.368-372.
Turnbough Jr, C.L. and Switzer, R.L., 2008. Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors. Microbiology and Molecular Biology Reviews, 72(2), pp.266-300.
Hoover TA, Roof WD, Foltermann KF, O'Donovan GA, Bencini DA, Wild JR: Nucleotide sequence of the structural gene (pyrB) that encodes the catalytic polypeptide of aspartate transcarbamoylase of Escherichia coli. Proc Natl Acad Sci U S A. 1983 May;80(9):2462-6. doi: 10.1073/pnas.80.9.2462.
Pubmed: 6302686
Roland KL, Liu CG, Turnbough CL Jr: Role of the ribosome in suppressing transcriptional termination at the pyrBI attenuator of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7149-53. doi: 10.1073/pnas.85.19.7149.
Pubmed: 2459698
Turnbough CL Jr, Hicks KL, Donahue JP: Attenuation control of pyrBI operon expression in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1983 Jan;80(2):368-72. doi: 10.1073/pnas.80.2.368.
Pubmed: 6300835
Cunin R, Jacobs A, Charlier D, Crabeel M, Herve G, Glansdorff N, Pierard A: Structure-function relationship in allosteric aspartate carbamoyltransferase from Escherichia coli. I. Primary structure of a pyrI gene encoding a modified regulatory subunit. J Mol Biol. 1985 Dec 20;186(4):707-13. doi: 10.1016/0022-2836(85)90390-0.
Pubmed: 3912513
Schachman HK, Pauza CD, Navre M, Karels MJ, Wu L, Yang YR: Location of amino acid alterations in mutants of aspartate transcarbamoylase: Structural aspects of interallelic complementation. Proc Natl Acad Sci U S A. 1984 Jan;81(1):115-9. doi: 10.1073/pnas.81.1.115.
Pubmed: 6364131
Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. Nucleic Acids Res. 1995 Jun 25;23(12):2105-19. doi: 10.1093/nar/23.12.2105.
Pubmed: 7610040
Ovchinnikov YA, Monastyrskaya GS, Gubanov VV, Guryev SO, Chertov OYu, Modyanov NN, Grinkevich VA, Makarova IA, Marchenko TV, Polovnikova IN, Lipkin VM, Sverdlov ED: The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit. Eur J Biochem. 1981 Jun 1;116(3):621-9. doi: 10.1111/j.1432-1033.1981.tb05381.x.
Pubmed: 6266829
Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL: Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 1993 Nov 25;21(23):5408-17. doi: 10.1093/nar/21.23.5408.
Pubmed: 8265357
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Meek DW, Hayward RS: Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4? Nucleic Acids Res. 1984 Jul 25;12(14):5813-21. doi: 10.1093/nar/12.14.5813.
Pubmed: 6379605
Igarashi K, Fujita N, Ishihama A: Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of Escherichia coli RNA polymerase. Nucleic Acids Res. 1990 Oct 25;18(20):5945-8. doi: 10.1093/nar/18.20.5945.
Pubmed: 2235479
Ovchinnikov YA, Lipkin VM, Modyanov NN, Chertov OY, Smirnov YV: Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli. FEBS Lett. 1977 Apr 1;76(1):108-11. doi: 10.1016/0014-5793(77)80131-2.
Pubmed: 323055
Ovchinnikov YuA, Monastyrskaya GS, Gubanov VV, Guryev SO, Salomatina IS, Shuvaeva TM, Lipkin VM, Sverdlov ED: The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit. Nucleic Acids Res. 1982 Jul 10;10(13):4035-44. doi: 10.1093/nar/10.13.4035.
Pubmed: 6287430
Squires C, Krainer A, Barry G, Shen WF, Squires CL: Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC). Nucleic Acids Res. 1981 Dec 21;9(24):6827-40. doi: 10.1093/nar/9.24.6827.
Pubmed: 6278450
Burton Z, Burgess RR, Lin J, Moore D, Holder S, Gross CA: The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12. Nucleic Acids Res. 1981 Jun 25;9(12):2889-903. doi: 10.1093/nar/9.12.2889.
Pubmed: 6269063
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
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