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Pathway Description
Operon: Glucuronate Pathway
Escherichia coli
Category:
Protein Pathway
Sub-Categories:
Gene Regulatory
Cellular Response
Created: 2015-11-10
Last Updated: 2026-05-29
The glucuronate pathway operon is a bicistronic operon consisting of the genes uxuA and uxuB. The transcription of this operon is activated by CRP-cAMP DNA-binding transcriptional dual regulator binding to -57.5 or -142.5 bp off the promoter. The transcription of the operon can be repressed by multiple means:
-UxuR (UxuR DNA-binding transcriptional repressor) binding -159.5 or -53.5 off the promoter
-ExuR (ExuR DNA-binding transcriptional repressor) binding to -159.5 or -53.5 off the promoter
-OxyR (OxyR DNA-binding transcriptional dual regulator) binding to -54 or -32 off the promoter
References
Operon: Glucuronate Pathway References
Bates Utz C, Nguyen AB, Smalley DJ, Anderson AB, Conway T: GntP is the Escherichia coli Fructuronic acid transporter and belongs to the UxuR regulon. J Bacteriol. 2004 Nov;186(22):7690-6. doi: 10.1128/JB.186.22.7690-7696.2004.
Pubmed: 15516583
Blanco C, Ritzenthaler P, Kolb A: The regulatory region of the uxuAB operon in Escherichia coli K12. Mol Gen Genet. 1986 Jan;202(1):112-9.
Pubmed: 3083215
Huerta AM, Collado-Vides J: Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals. J Mol Biol. 2003 Oct 17;333(2):261-78.
Pubmed: 14529615
Klemm P, Tong S, Nielsen H, Conway T: The gntP gene of Escherichia coli involved in gluconate uptake. J Bacteriol. 1996 Jan;178(1):61-7.
Pubmed: 8550444
Lynch AS, Lin EC: Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters. J Bacteriol. 1996 Nov;178(21):6238-49.
Pubmed: 8892825
Ritzenthaler P, Mata-Gilsinger M: Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression. J Bacteriol. 1982 Jun;150(3):1040-7.
Pubmed: 6281232
Ritzenthaler P, Blanco C, Mata-Gilsinger M: Genetic analysis of uxuR and exuR genes: evidence for ExuR and UxuR monomer repressors interactions. Mol Gen Genet. 1985;199(3):507-11.
Pubmed: 3929016
Robert-Baudouy J, Portalier R, Stoeber F: Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products. J Bacteriol. 1981 Jan;145(1):211-20.
Pubmed: 7007313
Rodionov DA, Mironov AA, Rakhmaninova AB, Gelfand MS: Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria. Mol Microbiol. 2000 Nov;38(4):673-83.
Pubmed: 11115104
Zheng M, Wang X, Templeton LJ, Smulski DR, LaRossa RA, Storz G: DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide. J Bacteriol. 2001 Aug;183(15):4562-70. doi: 10.1128/JB.183.15.4562-4570.2001.
Pubmed: 11443091
Zheng D, Constantinidou C, Hobman JL, Minchin SD: Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. Nucleic Acids Res. 2004 Nov 1;32(19):5874-93. doi: 10.1093/nar/gkh908. Print 2004.
Pubmed: 15520470
Ovchinnikov YA, Monastyrskaya GS, Gubanov VV, Guryev SO, Chertov OYu, Modyanov NN, Grinkevich VA, Makarova IA, Marchenko TV, Polovnikova IN, Lipkin VM, Sverdlov ED: The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit. Eur J Biochem. 1981 Jun 1;116(3):621-9. doi: 10.1111/j.1432-1033.1981.tb05381.x.
Pubmed: 6266829
Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL: Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 1993 Nov 25;21(23):5408-17. doi: 10.1093/nar/21.23.5408.
Pubmed: 8265357
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Meek DW, Hayward RS: Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4? Nucleic Acids Res. 1984 Jul 25;12(14):5813-21. doi: 10.1093/nar/12.14.5813.
Pubmed: 6379605
Igarashi K, Fujita N, Ishihama A: Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of Escherichia coli RNA polymerase. Nucleic Acids Res. 1990 Oct 25;18(20):5945-8. doi: 10.1093/nar/18.20.5945.
Pubmed: 2235479
Ovchinnikov YA, Lipkin VM, Modyanov NN, Chertov OY, Smirnov YV: Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli. FEBS Lett. 1977 Apr 1;76(1):108-11. doi: 10.1016/0014-5793(77)80131-2.
Pubmed: 323055
Ovchinnikov YuA, Monastyrskaya GS, Gubanov VV, Guryev SO, Salomatina IS, Shuvaeva TM, Lipkin VM, Sverdlov ED: The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit. Nucleic Acids Res. 1982 Jul 10;10(13):4035-44. doi: 10.1093/nar/10.13.4035.
Pubmed: 6287430
Squires C, Krainer A, Barry G, Shen WF, Squires CL: Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC). Nucleic Acids Res. 1981 Dec 21;9(24):6827-40. doi: 10.1093/nar/9.24.6827.
Pubmed: 6278450
Burton Z, Burgess RR, Lin J, Moore D, Holder S, Gross CA: The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12. Nucleic Acids Res. 1981 Jun 25;9(12):2889-903. doi: 10.1093/nar/9.12.2889.
Pubmed: 6269063
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. Nucleic Acids Res. 1995 Jun 25;23(12):2105-19. doi: 10.1093/nar/23.12.2105.
Pubmed: 7610040
Cossart P, Gicquel-Sanzey B: Cloning and sequence of the crp gene of Escherichia coli K 12. Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
Pubmed: 6280141
Aiba H, Fujimoto S, Ozaki N: Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 1982 Feb 25;10(4):1345-61. doi: 10.1093/nar/10.4.1345.
Pubmed: 6280140
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