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Pathway Description
N-Glycan Biosynthesis
Saccharomyces cerevisiae
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2016-03-08
Last Updated: 2024-11-18
The N-glycan biosynthesis is a pathway involving the creation of a dolichol, and the consecutive reactions involving the addition of Acetylglucosaminyl groups, mannosyl groups and glucosyl groups. The set of reactions all happen in the ER membrane. The resulting glucosyl3mannosyl9-N-acetylglucosaminyl2-diphosphodolichol is used as protein modificator.
References
N-Glycan Biosynthesis References
Bickel T, Lehle L, Schwarz M, Aebi M, Jakob CA: Biosynthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Alg13p and Alg14p form a complex required for the formation of GlcNAc(2)-PP-dolichol. J Biol Chem. 2005 Oct 14;280(41):34500-6. doi: 10.1074/jbc.M506358200. Epub 2005 Aug 12.
Pubmed: 16100113
Burda P, Aebi M: The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta. 1999 Jan 6;1426(2):239-57.
Pubmed: 9878760
Burda P, Jakob CA, Beinhauer J, Hegemann JH, Aebi M: Ordered assembly of the asymmetrically branched lipid-linked oligosaccharide in the endoplasmic reticulum is ensured by the substrate specificity of the individual glycosyltransferases. Glycobiology. 1999 Jun;9(6):617-25.
Pubmed: 10336995
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N: The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum. J Biol Chem. 2001 Jun 15;276(24):21828-40. doi: 10.1074/jbc.M010896200. Epub 2001 Feb 15.
Pubmed: 11278778
Herscovics A, Orlean P: Glycoprotein biosynthesis in yeast. FASEB J. 1993 Apr 1;7(6):540-50.
Pubmed: 8472892
Jakob CA, Burda P, Roth J, Aebi M: Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure. J Cell Biol. 1998 Sep 7;142(5):1223-33.
Pubmed: 9732283
Kukuruzinska MA, Bergh ML, Jackson BJ: Protein glycosylation in yeast. Annu Rev Biochem. 1987;56:915-44. doi: 10.1146/annurev.bi.56.070187.004411.
Pubmed: 3304149
Lehle L, Strahl S, Tanner W: Protein glycosylation, conserved from yeast to man: a model organism helps elucidate congenital human diseases. Angew Chem Int Ed Engl. 2006 Oct 20;45(41):6802-18. doi: 10.1002/anie.200601645.
Pubmed: 17024709
Moremen KW, Trimble RB, Herscovics A: Glycosidases of the asparagine-linked oligosaccharide processing pathway. Glycobiology. 1994 Apr;4(2):113-25.
Pubmed: 8054711
Heller L, Orlean P, Adair WL Jr: Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7013-6. doi: 10.1073/pnas.89.15.7013.
Pubmed: 1323123
Bernstein M, Kepes F, Schekman R: Sec59 encodes a membrane protein required for core glycosylation in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Mar;9(3):1191-9. doi: 10.1128/mcb.9.3.1191.
Pubmed: 2657387
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B: The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII. Nature. 1997 May 29;387(6632 Suppl):90-3.
Pubmed: 9169872
Hartog KO, Bishop B: Genomic sequence coding for tunicamycin resistance in yeast. Nucleic Acids Res. 1987 Apr 24;15(8):3627. doi: 10.1093/nar/15.8.3627.
Pubmed: 3033607
Feldmann H, Aigle M, Aljinovic G, Andre B, Baclet MC, Barthe C, Baur A, Becam AM, Biteau N, Boles E, Brandt T, Brendel M, Bruckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, Demolis N, Delaveau T, Doignon F, Domdey H, Dusterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstrom K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kallesoe T, Kiesau P, Kirchrath L, Kotter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, Muller S, Nasr F, Obermaier B, Perea J, Pierard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschlager I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K: Complete DNA sequence of yeast chromosome II. EMBO J. 1994 Dec 15;13(24):5795-809.
Pubmed: 7813418
Engel SR, Dietrich FS, Fisk DG, Binkley G, Balakrishnan R, Costanzo MC, Dwight SS, Hitz BC, Karra K, Nash RS, Weng S, Wong ED, Lloyd P, Skrzypek MS, Miyasato SR, Simison M, Cherry JM: The reference genome sequence of Saccharomyces cerevisiae: then and now. G3 (Bethesda). 2014 Mar 20;4(3):389-98. doi: 10.1534/g3.113.008995.
Pubmed: 24374639
Feuermann M, de Montigny J, Potier S, Souciet JL: The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes. Yeast. 1997 Jul;13(9):861-9. doi: 10.1002/(SICI)1097-0061(199707)13:9<861::AID-YEA125>3.0.CO;2-9.
Pubmed: 9234674
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Bruckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome VII. Nature. 1997 May 29;387(6632 Suppl):81-4.
Pubmed: 9169869
Van der Aart QJ, Barthe C, Doignon F, Aigle M, Crouzet M, Steensma HY: Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II. Yeast. 1994 Jul;10(7):959-64. doi: 10.1002/yea.320100711.
Pubmed: 7985423
Albright CF, Robbins RW: The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation. J Biol Chem. 1990 Apr 25;265(12):7042-9.
Pubmed: 2182636
Mannhaupt G, Stucka R, Ehnle S, Vetter I, Feldmann H: Analysis of a 70 kb region on the right arm of yeast chromosome II. Yeast. 1994 Oct;10(10):1363-81. doi: 10.1002/yea.320101014.
Pubmed: 7900426
Orlean P, Albright C, Robbins PW: Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein. J Biol Chem. 1988 Nov 25;263(33):17499-507.
Pubmed: 3053713
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Bruckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI. Nature. 1997 May 29;387(6632 Suppl):103-5.
Pubmed: 9169875
Jackson BJ, Kukuruzinska MA, Robbins P: Biosynthesis of asparagine-linked oligosaccharides in Saccharomyces cerevisiae: the alg2 mutation. Glycobiology. 1993 Aug;3(4):357-64. doi: 10.1093/glycob/3.4.357.
Pubmed: 8400550
Rieger M, Bruckner M, Schafer M, Muller-Auer S: Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII. Yeast. 1997 Sep 15;13(11):1077-90. doi: 10.1002/(SICI)1097-0061(19970915)13:11<1077::AID-YEA152>3.0.CO;2-Y.
Pubmed: 9290212
Bergez P, Doignon F, Crouzet M: The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae. Yeast. 1995 Aug;11(10):967-74. doi: 10.1002/yea.320111008.
Pubmed: 8533472
Bergez P, Doignon F, Crouzet M: Corrigendum to: the sequence of a 44 458 bp fragment located on the left arm of chromosome XIV from Saccharomyces cervisiae. Yeast. 1996 Mar 15;12(3):297. doi: 10.1002/(SICI)1097-0061(19960315)12:3%3C297::AID-YEA940%3E3.0.CO;2-D.
Pubmed: 8904343
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