Loading Pathway...
Error: Pathway image not found.
Hide
Pathway Description
Stress-Activated Signalling Pathways: Low Osmolarity
Saccharomyces cerevisiae
Category:
Protein Pathway
Sub-Categories:
Kinase Signaling
Stress-Activated Signaling
Cellular Response
Created: 2016-04-05
Last Updated: 2025-01-25
Stress-activated protein kinase pathways in Saccharomyces. The HOG1 MAPK pathway is controlled by two separate osmosensors, SLN1 and SHO1. Sln1 is activated in low osmolarity, thus repressing Ssk1 by phosphorylating it. Ssk1 is in charge of activating Ssk2/22 which in turn activates Pbs2 and in turn activates Hog1.
SHO1 is activated during high osmolarity, resulting in Ste11 being activated, which in turn activates Pbs2 and activates Hog1.
Ptp2 and Ptp3 negatively regulates Hog1.
References
Stress-Activated Signalling Pathways: Low Osmolarity References
Toone WM, Jones N: Stress-activated signalling pathways in yeast. Genes Cells. 1998 Aug;3(8):485-98.
Pubmed: 9797451
Posas F, Wurgler-Murphy SM, Maeda T, Witten EA, Thai TC, Saito H: Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 "two-component" osmosensor. Cell. 1996 Sep 20;86(6):865-75. doi: 10.1016/s0092-8674(00)80162-2.
Pubmed: 8808622
Lu JM, Deschenes RJ, Fassler JS: Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of Ssk1p and Skn7p. Eukaryot Cell. 2003 Dec;2(6):1304-14. doi: 10.1128/ec.2.6.1304-1314.2003.
Pubmed: 14665464
Xu Q, Porter SW, West AH: The yeast YPD1/SLN1 complex: insights into molecular recognition in two-component signaling systems. Structure. 2003 Dec;11(12):1569-81.
Pubmed: 14656441
Janiak-Spens F, Cook PF, West AH: Kinetic analysis of YPD1-dependent phosphotransfer reactions in the yeast osmoregulatory phosphorelay system. Biochemistry. 2005 Jan 11;44(1):377-86. doi: 10.1021/bi048433s.
Pubmed: 15628880
Xu Q, West AH: Conservation of structure and function among histidine-containing phosphotransfer (HPt) domains as revealed by the crystal structure of YPD1. J Mol Biol. 1999 Oct 8;292(5):1039-50. doi: 10.1006/jmbi.1999.3143.
Pubmed: 10512701
Maeda T, Takekawa M, Saito H: Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor. Science. 1995 Jul 28;269(5223):554-8. doi: 10.1126/science.7624781.
Pubmed: 7624781
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome V. Nature. 1997 May 29;387(6632 Suppl):78-81.
Pubmed: 9169868
Engel SR, Dietrich FS, Fisk DG, Binkley G, Balakrishnan R, Costanzo MC, Dwight SS, Hitz BC, Karra K, Nash RS, Weng S, Wong ED, Lloyd P, Skrzypek MS, Miyasato SR, Simison M, Cherry JM: The reference genome sequence of Saccharomyces cerevisiae: then and now. G3 (Bethesda). 2014 Mar 20;4(3):389-98. doi: 10.1534/g3.113.008995.
Pubmed: 24374639
Maeda T, Wurgler-Murphy SM, Saito H: A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature. 1994 May 19;369(6477):242-5. doi: 10.1038/369242a0.
Pubmed: 8183345
Johnston M, Hillier L, Riles L, Albermann K, Andre B, Ansorge W, Benes V, Bruckner M, Delius H, Dubois E, Dusterhoft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, Kotter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. Nature. 1997 May 29;387(6632 Suppl):87-90.
Pubmed: 9169871
Guan K, Deschenes RJ, Dixon JE: Isolation and characterization of a second protein tyrosine phosphatase gene, PTP2, from Saccharomyces cerevisiae. J Biol Chem. 1992 May 15;267(14):10024-30.
Pubmed: 1577774
Ota IM, Varshavsky A: A gene encoding a putative tyrosine phosphatase suppresses lethality of an N-end rule-dependent mutant. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2355-9. doi: 10.1073/pnas.89.6.2355.
Pubmed: 1549598
James P, Hall BD, Whelen S, Craig EA: Multiple protein tyrosine phosphatase-encoding genes in the yeast Saccharomyces cerevisiae. Gene. 1992 Dec 1;122(1):101-10. doi: 10.1016/0378-1119(92)90037-p.
Pubmed: 1452018
Guan KL, Deschenes RJ, Qiu H, Dixon JE: Cloning and expression of a yeast protein tyrosine phosphatase. J Biol Chem. 1991 Jul 15;266(20):12964-70.
Pubmed: 1649172
Jacq C, Alt-Morbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Blocker H, Blugeon C, Boskovic J, Brandt P, Bruckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome IV. Nature. 1997 May 29;387(6632 Suppl):75-8.
Pubmed: 9169867
Verhasselt P, Volckaert G: Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found. Yeast. 1997 Mar 15;13(3):241-50. doi: 10.1002/(SICI)1097-0061(19970315)13:3<241::AID-YEA61>3.0.CO;2-#.
Pubmed: 9090053
Wurgler-Murphy SM, Maeda T, Witten EA, Saito H: Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases. Mol Cell Biol. 1997 Mar;17(3):1289-97. doi: 10.1128/mcb.17.3.1289.
Pubmed: 9032256
Brewster JL, de Valoir T, Dwyer ND, Winter E, Gustin MC: An osmosensing signal transduction pathway in yeast. Science. 1993 Mar 19;259(5102):1760-3. doi: 10.1126/science.7681220.
Pubmed: 7681220
Boguslawski G: PBS2, a yeast gene encoding a putative protein kinase, interacts with the RAS2 pathway and affects osmotic sensitivity of Saccharomyces cerevisiae. J Gen Microbiol. 1992 Nov;138(11):2425-32. doi: 10.1099/00221287-138-11-2425.
Pubmed: 1479360
Boguslawski G, Polazzi JO: Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5848-52. doi: 10.1073/pnas.84.16.5848.
Pubmed: 3039511
Highlighted elements will appear in red.
Highlight Compounds
Highlight Proteins
Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.
Visualize Compound Data
Visualize Protein Data
Downloads
Settings