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Endoplasmic Reticulum acetyl-CoA C- acetyltransferase Hydroxymethylglutaryl- CoA synthase 3-hydroxy-3- methylglutaryl- coenzyme A reductase 1 3-hydroxy-3- methylglutaryl- coenzyme A reductase 2 mevalonate kinase Phosphomevalonate kinase Diphosphomevalonate decarboxylase Isopentenyl- diphosphate Delta-isomerase Farnesyl pyrophosphate synthase Farnesyl pyrophosphate synthase Squalene synthase squalene monooxygenase Lanosterol synthase Lanosterol 14-alpha demethylase Delta(14)- sterol reductase Methylsterol monooxygenase Methylsterol monooxygenase Methylsterol monooxygenase C-3 sterol dehydrogenase 3-keto sterol reductase Methylsterol monooxygenase Methylsterol monooxygenase Methylsterol monooxygenase C-3 sterol dehydrogenase 3-keto sterol reductase Sterol 24-C- methyltransferase C-8 sterol isomerase C-5 sterol desaturase C-22 sterol desaturase C-24 sterol reductase C-8 sterol isomerase C-5 sterol desaturase Cholesterol Decanoyl-CoA CoA CE(10:0) Acetyl-CoA CoA Acetoacetyl-CoA Acetyl-CoA Acetoacetyl-CoA H2O 3-Hydroxy-3- methylglutaryl- CoA CoA NADPH Mevalonic acid NADP CoA Mevalonic acid ATP Mevalonic acid-5P ADP ATP Mevalonic acid-5P ADP (R)-5- Diphosphomevalonic acid ATP ADP Pi Isopentenyl pyrophosphate CO2 Isopentenyl pyrophosphate Dimethylallylpyrophosphate Isopentenyl pyrophosphate Geranyl-PP PPi Isopentenyl pyrophosphate Farnesyl pyrophosphate PPi NADPH Squalene NADP PPi Squalene O2 NADPH (S)-2,3- Epoxysqualene H2O NADP Lanosterol Lanosterol O2 NADPH 4,4- Dimethylcholesta- 8,14,24- trienol Formic acid NADP H2O NADPH 4,4-Dimethyl- 5a-cholesta- 8,24-dien-3- b-ol NADP 4,4-Dimethyl- 5a-cholesta- 8,24-dien-3- b-ol H+ O2 NADPH 4α- hydroxymethyl- 4β-methyl-5α- cholesta- 8,24-dien-3β- ol NADP H2O H+ O2 NADPH 4α-formyl-4β- methyl-5α- cholesta- 8,24-dien-3β- ol H2O NADP O2 NADPH 4β-methyl-4α- carboxy- cholesta- 8,24-dien-3β- ol H2O NADP NADP 3-Keto-4- methylzymosterol CO2 NADPH NADPH 4a- Methylzymosterol NADP H+ O2 NADPH H2O NADP 4α- hydroxymethyl- 5α-cholesta- 8,24-dien-3β- ol H+ O2 NADPH 4α-formyl-5α- cholesta- 8,24-dien-3β- ol H2O NADP O2 NADPH 4α-carboxy- 5α-cholesta- 8,24-dien-3β- ol H2O NADP NADP 5α-cholesta- 8,24-dien-3- one CO2 NADPH NADPH H+ Zymosterol NADP S- Adenosylmethionine fecosterol S- Adenosylhomocysteine H+ Episterol H+ O2 ferrocytochrome c 5,7,24(28)- Ergostatrienol H2O ferricytochrome c H+ NADPH O2 ergosta- 5,7,22,24(28)- tetraen-3-β- ol NADP H2O H+ NADPH Ergosterol NADP 5a-cholesta- 7,24-dien-3B- ol 7- Dehydrodesmosterol Desmosterol Cholesterol