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Sodium/chloride cotransporter Aquaporin-2 Aquaporin-2 Transient receptor potential cation channel subfamily V member 5 ATP-binding cassette sub-family C member 8 ATP-sensitive inward rectifier potassium channel 8 ATP-sensitive inward rectifier potassium channel 11 ATP-binding cassette sub-family C member 9 Sodium/calcium exchanger 3 Chloride channel protein ClC-Ka Sodium/potassium- transporting ATPase subunit alpha-1 Sodium/potassium- transporting ATPase subunit alpha-2 Sodium/potassium- transporting ATPase subunit alpha-3 Sodium/potassium- transporting ATPase subunit alpha-4 Sodium/potassium- transporting ATPase subunit beta-1 Sodium/potassium- transporting ATPase subunit beta-2 Sodium/potassium- transporting ATPase subunit beta-3 ATPase, (Na+)/K+ transporting, beta 4 polypeptide Sodium/potassium- transporting ATPase subunit gamma Indapamide transporter Indapamide H2O H2O H2O Ca+ Ca+ K+ K+ Na+ Na+ Cl- Cl- Ca+ Ca+ Na+ Na+ Cl- Cl- Na+ Na+ K+ K+ Indapamide Indapamide Distal convoluted tubule Lumen Indapamide inhibits the sodium/chloride transporter Cytosolic Na+ decreases, thus, less Na+ is pumped into the blood Indapamide is transported from the blood into the epithelial cells of the distal convoluted tubule Epithelial cell The concentration of Na+ and Cl- builds up in the lumen/urine. The concentration gradient between the epithelial cells and the lumen is decreased. Due to the high concentration of solutes in the filtrate, more water moves into the lumen and is excreted. Blood Vessel Kidney
SLC12A3 AQP2 AQP2 TRPV5 ABCC8 KCNJ8 KCNJ11 Unknown SLC8A3 CLCNKA ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 Unknown FXYD2 Unknown Indapamide Water Water Water Calcium Calcium Potassium Potassium Sodium Sodium Chloride ion Chloride ion Calcium Calcium Sodium Sodium Chloride ion Chloride ion Sodium Sodium Potassium Potassium Indapamide Indapamide
SLC12A3 AQP2 AQP2 TRPV5 ABCC8 KCNJ8 KCNJ11 SLC8A3 CLCNKA ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 FXYD2 Indapam H2O H2O H2O Ca+ Ca+ K+ K+ Na+ Na+ Cl- Cl- Ca+ Ca+ Na+ Na+ Cl- Cl- Na+ Na+ K+ K+ Indapam Indapam Distal convoluted tubule Lumen Indapamide inhibits the sodium/chloride transporter Cytosolic Na+ decreases, thus, less Na+ is pumped into the blood Indapamide is transported from the blood into the epithelial cells of the distal convoluted tubule Epithelial cell The concentration of Na+ and Cl- builds up in the lumen/urine. The concentration gradient between the epithelial cells and the lumen is decreased. Due to the high concentration of solutes in the filtrate, more water moves into the lumen and is excreted. Blood Vessel Kidney
SLC12A3 AQP2 AQP2 TRPV5 ABCC8 KCNJ8 KCNJ11 SLC8A3 CLCNKA ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 FXYD2 Indapam H2O H2O H2O Ca2+ Ca2+ K+ K+ Na+ Na+ Cl Cl Ca2+ Ca2+ Na+ Na+ Cl Cl Na+ Na+ K+ K+ Indapam Indapam