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Sodium/potassium- transporting ATPase subunit beta-1 Sodium/potassium- transporting ATPase subunit gamma Sodium/potassium- transporting ATPase subunit beta-3 Solute carrier family 12 member 1 Sodium/potassium- transporting ATPase subunit alpha-3 Sodium/potassium- transporting ATPase subunit beta-2 ATPase, (Na+)/K+ transporting, beta 4 polypeptide Solute carrier family 12 member 6 Potassium channel subfamily K member 1 Chloride channel protein ClC-Ka Ethacrynic acid transporter Ethacrynic acid transporter Sodium/potassium- transporting ATPase subunit alpha-2 Sodium/potassium- transporting ATPase subunit alpha-4 Sodium/potassium- transporting ATPase subunit alpha-1 Ethacrynic acid Na+ Na+ K+ K+ K+ K+ Cl- Cl- Na+ Na+ K+ K+ Cl- Cl- K+ K+ Cl- Cl- Ethacrynic acid Ethacrynic acid Lumen Loop of Henle Concentrations of sodium. chloride and potassium increases in the lumen. The concentration gradient across the lumen and epithelial cell of the loop of henle is decreased. This results in decreased water re-absorption from the lumen, causing more water to be excreted in urine. Ethacrynic acid competes with chloride in the NKCC2 co-transporter on the luminal membrane of the ascending limb of the loop of henle. It blocks NKCC2 preventing reabsorption of Na+, K+ and Cl- Ethacrynic acid is transported from the blood into the epithelial cell and then transported into the lumen of the kidney Peritubular fluid Cytosol
ATP1B1 FXYD2 ATP1B3 SLC12A1 ATP1A3 ATP1B2 Unknown SLC12A6 KCNK1 CLCNKA Unknown Unknown ATP1A2 ATP1A4 ATP1A1 Ethacrynic acid Sodium Sodium Potassium Potassium Potassium Potassium Chloride ion Chloride ion Sodium Sodium Potassium Potassium Chloride ion Chloride ion Potassium Potassium Chloride ion Chloride ion Ethacrynic acid Ethacrynic acid
ATP1B1 FXYD2 ATP1B3 SLC12A1 ATP1A3 ATP1B2 SLC12A6 KCNK1 CLCNKA ATP1A2 ATP1A4 ATP1A1 Ethacry Na+ Na+ K+ K+ K+ K+ Cl- Cl- Na+ Na+ K+ K+ Cl- Cl- K+ K+ Cl- Cl- Ethacry Ethacry Lumen Loop of Henle Concentrations of sodium. chloride and potassium increases in the lumen. The concentration gradient across the lumen and epithelial cell of the loop of henle is decreased. This results in decreased water re-absorption from the lumen, causing more water to be excreted in urine. Ethacrynic acid competes with chloride in the NKCC2 co-transporter on the luminal membrane of the ascending limb of the loop of henle. It blocks NKCC2 preventing reabsorption of Na+, K+ and Cl- Ethacrynic acid is transported from the blood into the epithelial cell and then transported into the lumen of the kidney Peritubular fluid Cytosol
ATP1B1 FXYD2 ATP1B3 SLC12A1 ATP1A3 ATP1B2 SLC12A6 KCNK1 CLCNKA ATP1A2 ATP1A4 ATP1A1 Ethacry Na+ Na+ K+ K+ K+ K+ Cl Cl Na+ Na+ K+ K+ Cl Cl K+ K+ Cl Cl Ethacry Ethacry