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Ca+ Protein kinase C alpha type Voltage- dependent P/Q-type calcium channel subunit alpha-1A Voltage- dependent L-type calcium channel subunit beta-1 Voltage- dependent calcium channel subunit alpha-2/delta-2 Gq protein signaling cascade Phospholipase C Inositol 1,4,5- trisphosphate receptor type 1 Myosin light chain kinase, smooth muscle Tropomyosin alpha-1 chain Tropomyosin beta chain Alpha-1A adrenergic receptor Beta-1 adrenergic receptor Myosin LC-P Myosin light chain 3 Voltage- dependent L-type calcium channel subunit alpha-1C Voltage- dependent calcium channel subunit alpha-2/delta-2 Voltage- dependent L-type calcium channel subunit beta-1 Ryanodine receptor 2 cAMP-dependent protein kinase type I-alpha regulatory subunit Sodium/calcium exchanger 1 Voltage- dependent T-type calcium channel subunit alpha-1H Voltage- dependent T-type calcium channel subunit alpha-1G Serine/threonine- protein phosphatase PP1-beta catalytic subunit Calmodulin-1 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Calmodulin-1 cAMP-dependent protein kinase catalytic subunit alpha Inositol 1,4,5-trisphosphate Norepinephrine Norepinephrine Ca+ Ca+ Ca+ Ca+ Ca+ Na+ Na+ Ca+ Ca+ Ca+ Ca+ ATP Phosphatidylinositol 4,5-bisphosphate ATP H2O Ca+ ADP Pi Ca+ Ca+ Ca+ Diacylglycerol Troponin Troponin Muscle Contraction Muscle Relaxation Muscle Contraction G-protein signalling cascade Calcium Magnesium Calcium Manganese Sarcoplasmic Reticulum Increased calcium binds readily to calmodulin to activate myosin light chain kinase There is an overall increase in calcium levels in the cytosol caused by the activation of the Gq signaling cascade Cytosol Myosin binds to actin causing muscle contraction Activation of myosin light chain kinase leads to high concentration of myosin LC-P which causes contractions of the muscles Smooth Muscle of Blood Vessel Cardiomyocyte Cytosol Sarcoplasmic Reticulum Calcium binding to troponin displaces tropomyosin from the myosin binding sites on the actin filaments Myosin, with an ADP and phosphate attached, binds to actin to form a cross bridge. Myosin performs a powerstroke, drawing the actin filaments together. Many actin filaments pulled together at the same time leads to muscle contraction. Actin Filament Myosin Filament Single Sarcomere Functioning Unit The beta-1 adrenergic receptor is coupled to and activates the G-protein signalling cascade. the G-protein signalling cascade activates protein kinase which activates calcium channels on the membrane, resulting in calcium influx. Calcium activates the ryanodine receptor on the sarcoplasmic reticulum.
Calcium PRKCA CACNA1A CACNB1 CACNA2D2 GNAQ PLCB1 ITPR1 MYLK TPM1 TPM2 ADRA1A ADRB1 MYL3 MYL3 CACNA1C CACNA2D2 CACNB1 RYR2 PRKAR1A SLC8A1 CACNA1H CACNA1G PPP1CB CALM1 ATP2A2 CALM1 PRKACA Inositol 1,4,5- trisphosphate Norepinephrine Norepinephrine Calcium Calcium Calcium Calcium Calcium Sodium Sodium Calcium Calcium Calcium Calcium Adenosine triphosphate Phosphatidylinositol 4,5- bisphosphate Adenosine triphosphate Water Calcium Adenosine diphosphate Phosphate Calcium Calcium Calcium Diacylglycerol Troponin Troponin Muscle Contraction Muscle Relaxation Muscle Contraction G-protein signalling cascade
Ca+ PRKCA CACNA1A CACNB1 CACNA2D2 GNAQ PLCB1 ITPR1 MYLK TPM1 TPM2 ADRA1A ADRB1 MYL3 MYL3 CACNA1C CACNA2D2 CACNB1 RYR2 PRKAR1A SLC8A1 CACNA1H CACNA1G PPP1CB CALM1 ATP2A2 CALM1 PRKACA Inotp Norpp Norpp Ca+ Ca+ Ca+ Ca+ Ca+ Na+ Na+ Ca+ Ca+ Ca+ Ca+ ATP P45P ATP H2O Ca+ ADP Pi Ca+ Ca+ Ca+ Diacylg Troponi Troponi Mus Con Mus Rel Mus Con G-p s c Ca2+ Mg2+ Ca2+ Mn2+ Sarcoplasmic Reticulum Increased calcium binds readily to calmodulin to activate myosin light chain kinase There is an overall increase in calcium levels in the cytosol caused by the activation of the Gq signaling cascade Cytosol Myosin binds to actin causing muscle contraction Activation of myosin light chain kinase leads to high concentration of myosin LC-P which causes contractions of the muscles Smooth Muscle of Blood Vessel Cardiomyocyte Cytosol Sarcoplasmic Reticulum Calcium binding to troponin displaces tropomyosin from the myosin binding sites on the actin filaments Myosin, with an ADP and phosphate attached, binds to actin to form a cross bridge. Myosin performs a powerstroke, drawing the actin filaments together. Many actin filaments pulled together at the same time leads to muscle contraction. Actin Filament Myosin Filament Single Sarcomere Functioning Unit The beta-1 adrenergic receptor is coupled to and activates the G-protein signalling cascade. the G-protein signalling cascade activates protein kinase which activates calcium channels on the membrane, resulting in calcium influx. Calcium activates the ryanodine receptor on the sarcoplasmic reticulum.
Ca2+ PRKCA CACNA1A CACNB1 CACNA2D2 GNAQ PLCB1 ITPR1 MYLK TPM1 TPM2 ADRA1A ADRB1 MYL3 MYL3 CACNA1C CACNA2D2 CACNB1 RYR2 PRKAR1A SLC8A1 CACNA1H CACNA1G PPP1CB CALM1 ATP2A2 CALM1 PRKACA Inotp Norpp Norpp Ca2+ Ca2+ Ca2+ Ca2+ Ca2+ Na+ Na+ Ca2+ Ca2+ Ca2+ Ca2+ ATP P45P ATP H2O Ca2+ ADP Pi Ca2+ Ca2+ Ca2+ Diacylg Troponi Troponi Mus Con Mus Rel Mus Con G-p s c