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Pathway Description
Aspartate Metabolism
Escherichia coli O157:H7 str. TW14359
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2024-12-16
Last Updated: 2025-08-08
Aspartate is synthesized from and broken down to oxaloacetate, a TCA cycle intermediate, via a reversible transamination reaction with glutamate. This reaction is catalyzed by the aminotransferase AspC or TyrB. Aspartate is a component of proteins and is involved in many biosyntheses pathways like NAD biosynthesis and beta-alanine metabolism. Aspartate can also be synthesized from fumaric acid through an aspartate ammonia lyase. Aspartate also participates in the synthesis of L-asparagine through two different methods, either through aspartate ammonia ligase or asparagine synthetase B. Aspartate is also a precursor of fumaric acid. Again it has two possible ways of synthesizing it. First set of reactions follows an adenylo succinate synthetase that yields adenylsuccinic acid and then adenylosuccinate lyase in turns leads to fumaric acid. The second way is through argininosuccinate synthase that yields argininosuccinic acid and then argininosuccinate lyase in turns leads to fumaric acid.
References
Aspartate Metabolism References
Scofield MA, Lewis WS, Schuster SM: Nucleotide sequence of Escherichia coli asnB and deduced amino acid sequence of asparagine synthetase B. J Biol Chem. 1990 Aug 5;265(22):12895-902.
Pubmed: 1973930
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.
Pubmed: 8905232
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Flachmann R, Kunz N, Seifert J, Gutlich M, Wientjes FJ, Laufer A, Gassen HG: Molecular biology of pyridine nucleotide biosynthesis in Escherichia coli. Cloning and characterization of quinolinate synthesis genes nadA and nadB. Eur J Biochem. 1988 Aug 1;175(2):221-8. doi: 10.1111/j.1432-1033.1988.tb14187.x.
Pubmed: 2841129
Seifert J, Kunz N, Flachmann R, Laufer A, Jany KD, Gassen HG: Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase. Biol Chem Hoppe Seyler. 1990 Mar;371(3):239-48.
Pubmed: 2187483
Mattevi A, Tedeschi G, Bacchella L, Coda A, Negri A, Ronchi S: Structure of L-aspartate oxidase: implications for the succinate dehydrogenase/fumarate reductase oxidoreductase family. Structure. 1999 Jul 15;7(7):745-56.
Pubmed: 10425677
Woods SA, Miles JS, Roberts RE, Guest JR: Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12. Biochem J. 1986 Jul 15;237(2):547-57. doi: 10.1042/bj2370547.
Pubmed: 3541901
Takagi JS, Ida N, Tokushige M, Sakamoto H, Shimura Y: Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W. Nucleic Acids Res. 1985 Mar 25;13(6):2063-74. doi: 10.1093/nar/13.6.2063.
Pubmed: 2987841
Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. Nucleic Acids Res. 1995 Jun 25;23(12):2105-19. doi: 10.1093/nar/23.12.2105.
Pubmed: 7610040
Wolfe SA, Smith JM: Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12. J Biol Chem. 1988 Dec 15;263(35):19147-53.
Pubmed: 3058695
Van Vliet F, Crabeel M, Boyen A, Tricot C, Stalon V, Falmagne P, Nakamura Y, Baumberg S, Glansdorff N: Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisiae: comparison with methanogenic archaebacteria and mammals. Gene. 1990 Oct 30;95(1):99-104. doi: 10.1016/0378-1119(90)90419-r.
Pubmed: 2123815
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL: Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 1993 Nov 25;21(23):5408-17. doi: 10.1093/nar/21.23.5408.
Pubmed: 8265357
Cunin R, Jacobs A, Charlier D, Crabeel M, Herve G, Glansdorff N, Pierard A: Structure-function relationship in allosteric aspartate carbamoyltransferase from Escherichia coli. I. Primary structure of a pyrI gene encoding a modified regulatory subunit. J Mol Biol. 1985 Dec 20;186(4):707-13. doi: 10.1016/0022-2836(85)90390-0.
Pubmed: 3912513
Schachman HK, Pauza CD, Navre M, Karels MJ, Wu L, Yang YR: Location of amino acid alterations in mutants of aspartate transcarbamoylase: Structural aspects of interallelic complementation. Proc Natl Acad Sci U S A. 1984 Jan;81(1):115-9. doi: 10.1073/pnas.81.1.115.
Pubmed: 6364131
Hoover TA, Roof WD, Foltermann KF, O'Donovan GA, Bencini DA, Wild JR: Nucleotide sequence of the structural gene (pyrB) that encodes the catalytic polypeptide of aspartate transcarbamoylase of Escherichia coli. Proc Natl Acad Sci U S A. 1983 May;80(9):2462-6. doi: 10.1073/pnas.80.9.2462.
Pubmed: 6302686
Roland KL, Liu CG, Turnbough CL Jr: Role of the ribosome in suppressing transcriptional termination at the pyrBI attenuator of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7149-53. doi: 10.1073/pnas.85.19.7149.
Pubmed: 2459698
Turnbough CL Jr, Hicks KL, Donahue JP: Attenuation control of pyrBI operon expression in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1983 Jan;80(2):368-72. doi: 10.1073/pnas.80.2.368.
Pubmed: 6300835
Kuramitsu S, Okuno S, Ogawa T, Ogawa H, Kagamiyama H: Aspartate aminotransferase of Escherichia coli: nucleotide sequence of the aspC gene. J Biochem. 1985 Apr;97(4):1259-62. doi: 10.1093/oxfordjournals.jbchem.a135173.
Pubmed: 3897210
Fotheringham IG, Dacey SA, Taylor PP, Smith TJ, Hunter MG, Finlay ME, Primrose SB, Parker DM, Edwards RM: The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes. Biochem J. 1986 Mar 15;234(3):593-604. doi: 10.1042/bj2340593.
Pubmed: 3521591
Kondo K, Wakabayashi S, Yagi T, Kagamiyama H: The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes. Biochem Biophys Res Commun. 1984 Jul 18;122(1):62-7. doi: 10.1016/0006-291x(84)90439-x.
Pubmed: 6378205
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000809
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