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Pathway Description
Secondary Metabolites: Trehalose Biosynthesis and Metabolism
Escherichia coli (strain K12)
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2025-01-18
Last Updated: 2025-01-18
Threhalose biosynthesis begins with an Alpha-D-glucose-1-phosphate interacting with an ATP through a glucose-1-phosphate adenylyltransferase resulting in the release of a pyrophosphate and an ADP-glucose. The latter compound interacts in a reversible reaction with an amylose through a glycogen synthase resulting in the release of an ADP and an amylose. Amylose then interacts in a reversible reaction with 1,4-α-glucan branching enzyme resulting in a glycogen
Glycogen can also be produced by a reversible reaction with Amylose through a maltodextrin phosphorylase, releasing a phosphate and a glycogen.
Glycogen is then transformed into trehalose through a glycogen debranching enzyme. Alpha Alpha Trehalose can be degraded by reacting with with a water molecule through a cytoplasmic trehalase resulting in the release of a Beta-D-glucose and an Alpha-D-glucose.phosphorylated resulting in a Beta-D-glucose 6-phosphate. This compound is phosphorylated and can then join glycolysis
Alpha Alpha Trehalose can be degraded in the periplasmic space by reacting with with a water molecule through a periplasmic trehalase resulting in the release of a Beta-D-glucose and an Alpha-D-glucose.
The beta-D-glucose can be transported into the cytosol through a PTS permease where it is phosphorylated resulting in a Beta-D-glucose 6-phosphate. This compound can then join glycolysis
References
Secondary Metabolites: Trehalose Biosynthesis and Metabolism References
Ghosh P, Meyer C, Remy E, Peterson D, Preiss J: Cloning, expression, and nucleotide sequence of glgC gene from an allosteric mutant of Escherichia coli B. Arch Biochem Biophys. 1992 Jul;296(1):122-8. doi: 10.1016/0003-9861(92)90553-9.
Pubmed: 1339262
Meyer CR, Ghosh P, Remy E, Preiss J: Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B. J Bacteriol. 1992 Jul;174(13):4509-12. doi: 10.1128/jb.174.13.4509-4512.1992.
Pubmed: 1320612
Baecker PA, Furlong CE, Preiss J: Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose synthetase as deduced from the nucleotide sequence of the glg C gene. J Biol Chem. 1983 Apr 25;258(8):5084-8.
Pubmed: 6300111
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000984
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