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Pathway Description
Secondary Metabolites: Trehalose Biosynthesis and Metabolism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2025-01-18
Last Updated: 2025-01-18
Threhalose biosynthesis begins with an Alpha-D-glucose-1-phosphate interacting with an ATP through a glucose-1-phosphate adenylyltransferase resulting in the release of a pyrophosphate and an ADP-glucose. The latter compound interacts in a reversible reaction with an amylose through a glycogen synthase resulting in the release of an ADP and an amylose. Amylose then interacts in a reversible reaction with 1,4-α-glucan branching enzyme resulting in a glycogen
Glycogen can also be produced by a reversible reaction with Amylose through a maltodextrin phosphorylase, releasing a phosphate and a glycogen.
Glycogen is then transformed into trehalose through a glycogen debranching enzyme. Alpha Alpha Trehalose can be degraded by reacting with with a water molecule through a cytoplasmic trehalase resulting in the release of a Beta-D-glucose and an Alpha-D-glucose.phosphorylated resulting in a Beta-D-glucose 6-phosphate. This compound is phosphorylated and can then join glycolysis
Alpha Alpha Trehalose can be degraded in the periplasmic space by reacting with with a water molecule through a periplasmic trehalase resulting in the release of a Beta-D-glucose and an Alpha-D-glucose.
The beta-D-glucose can be transported into the cytosol through a PTS permease where it is phosphorylated resulting in a Beta-D-glucose 6-phosphate. This compound can then join glycolysis
References
Secondary Metabolites: Trehalose Biosynthesis and Metabolism References
Meyer D, Schneider-Fresenius C, Horlacher R, Peist R, Boos W: Molecular characterization of glucokinase from Escherichia coli K-12. J Bacteriol. 1997 Feb;179(4):1298-306. doi: 10.1128/jb.179.4.1298-1306.1997.
Pubmed: 9023215
Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. doi: 10.1093/dnares/4.2.91.
Pubmed: 9205837
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Erni B, Zanolari B: Glucose-permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIGlc. J Biol Chem. 1986 Dec 15;261(35):16398-403.
Pubmed: 3023349
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.
Pubmed: 8905232
De Reuse H, Danchin A: The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription. J Bacteriol. 1988 Sep;170(9):3827-37. doi: 10.1128/jb.170.9.3827-3837.1988.
Pubmed: 2457575
Saffen DW, Presper KA, Doering TL, Roseman S: Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes. J Biol Chem. 1987 Nov 25;262(33):16241-53.
Pubmed: 2960675
Yang Y, Zhao G, Winkler ME: Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12. FEMS Microbiol Lett. 1996 Jul 15;141(1):89-95. doi: 10.1111/j.1574-6968.1996.tb08368.x.
Pubmed: 8764513
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000984
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