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Pathway Description
S-Adenosyl-L-Methionine Biosynthesis
Escherichia coli E24377A
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2025-01-21
Last Updated: 2025-06-11
S-adenosyl-L-methionine biosynthesis(SAM) is synthesized in the cytosol of the cell from L-methionine and ATP. This reaction is catalyzed by methionine adenosyltransferase. L methione is taken up from the environment through a complex reaction coupled transport and then proceeds too synthesize the s adenosylmethionine through a adenosylmethionine synthase. S-adenosylmethionine then interacts with a hydrogen ion through an adenosylmethionine decarboxylase resulting in a carbon dioxide and a S-adenosyl 3-methioninamine. This compound interacts with a putrescine through a spermidine synthase resulting in a spermidine, a hydrogen ion and a S-methyl-5'-thioadenosine. The latter compound is degraded by interacting with a water molecule through a 5' methylthioadenosine nucleosidase resulting in an adenine and a S-methylthioribose which is then release into the environment
References
S-Adenosyl-L-Methionine Biosynthesis References
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Pubmed: 3316212
Fujita N, Mori H, Yura T, Ishihama A: Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region. Nucleic Acids Res. 1994 May 11;22(9):1637-9. doi: 10.1093/nar/22.9.1637.
Pubmed: 8202364
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Pubmed: 9278503
Xie QW, Tabor CW, Tabor H: Spermidine biosynthesis in Escherichia coli: promoter and termination regions of the speED operon. J Bacteriol. 1989 Aug;171(8):4457-65. doi: 10.1128/jb.171.8.4457-4465.1989.
Pubmed: 2666401
Tabor CW, Tabor H, Xie QW: Spermidine synthase of Escherichia coli: localization of the speE gene. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6040-4. doi: 10.1073/pnas.83.16.6040.
Pubmed: 3526348
Cornell KA, Riscoe MK: Cloning and expression of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: identification of the pfs gene product. Biochim Biophys Acta. 1998 Mar 4;1396(1):8-14. doi: 10.1016/s0167-4781(97)00169-3.
Pubmed: 9524204
Cornell KA, Swarts WE, Barry RD, Riscoe MK: Characterization of recombinant Eschericha coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: analysis of enzymatic activity and substrate specificity. Biochem Biophys Res Commun. 1996 Nov 21;228(3):724-32. doi: 10.1006/bbrc.1996.1723.
Pubmed: 8941345
Wurgler SM, Richardson CC: Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2740-4. doi: 10.1073/pnas.87.7.2740.
Pubmed: 2157212
Fukuchi J, Kashiwagi K, Takio K, Igarashi K: Properties and structure of spermidine acetyltransferase in Escherichia coli. J Biol Chem. 1994 Sep 9;269(36):22581-5.
Pubmed: 8077207
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. doi: 10.1093/dnares/3.6.363.
Pubmed: 9097039
Markham GD, DeParasis J, Gatmaitan J: The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli. J Biol Chem. 1984 Dec 10;259(23):14505-7.
Pubmed: 6094561
Satishchandran C, Taylor JC, Markham GD: Isozymes of S-adenosylmethionine synthetase are encoded by tandemly duplicated genes in Escherichia coli. Mol Microbiol. 1993 Aug;9(4):835-46. doi: 10.1111/j.1365-2958.1993.tb01742.x.
Pubmed: 8231813
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
Allikmets R, Gerrard B, Court D, Dean M: Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance. Gene. 1993 Dec 22;136(1-2):231-6. doi: 10.1016/0378-1119(93)90470-n.
Pubmed: 7904973
Gal J, Szvetnik A, Schnell R, Kalman M: The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster. J Bacteriol. 2002 Sep;184(17):4930-2. doi: 10.1128/jb.184.17.4930-4932.2002.
Pubmed: 12169620
Gervais FG, Drapeau GR: Identification, cloning, and characterization of rcsF, a new regulator gene for exopolysaccharide synthesis that suppresses the division mutation ftsZ84 in Escherichia coli K-12. J Bacteriol. 1992 Dec;174(24):8016-22. doi: 10.1128/jb.174.24.8016-8022.1992.
Pubmed: 1459951
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000857
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