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Pathway Description
Asparagine degradation: ansA, ansB
Escherichia coli K-12
Category:
Metabolite Pathway
Sub-Category:
Signaling
Created: 2025-03-04
Last Updated: 2025-05-08
The regulation of the ansAB operon. The operon is inhibited by ansR (HTH-type transcriptional regulator) and activated by high asparagine concentrations within the cell. AnsR binds to the promoter when asparagine is present in low concentrations and ammonium is present in high concentrations (by product of asparagine degradation). Asparagine is transported into the cell when concentrations are low within the cell. Once asparagine is present in high concentrations, ansR releases the promoter allowing crp to bind to the promoter and complete transcription. The products of the operon are two enzymes: ansA (L-asparaginase 1) and ansB (L-asparaginase 2). Both enzymes are used to degrade asparagine. The difference between the two enzymes is the location in which asparagine is degraded. AnsA degrades asparagine in the cytoplasm and ansB degrades asparagine in the periplasm.
References
Asparagine degradation: ansA, ansB References
Meissner J, Konigshof M, Wrede K, Warneke R, Mardoukhi MSY, Commichau FM, Stulke J: Control of asparagine homeostasis in Bacillus subtilis: identification of promiscuous amino acid importers and exporters. J Bacteriol. 2024 Feb 22;206(2):e0042023. doi: 10.1128/jb.00420-23. Epub 2024 Jan 9.
Pubmed: 38193659
Sabag-Daigle A, Sengupta A, Blunk HM, Biswas PK, Cron MC, Bogard AJ, Behrman EJ, Gopalan V, Ahmer BMM: Salmonella FraE, an Asparaginase Homolog, Contributes to Fructose-Asparagine but Not Asparagine Utilization. J Bacteriol. 2017 Oct 17;199(22):e00330-17. doi: 10.1128/JB.00330-17. Print 2017 Nov 15.
Pubmed: 28847920
Jennings MP, Beacham IR: Co-dependent positive regulation of the ansB promoter of Escherichia coli by CRP and the FNR protein: a molecular analysis. Mol Microbiol. 1993 Jul;9(1):155-64. doi: 10.1111/j.1365-2958.1993.tb01677.x.
Pubmed: 8412660
Fisher SH, Wray LV Jr. Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase. J Bacteriol. 2002 Apr;184(8):2148-54. doi: 10.1128/JB.184.8.2148-2154.2002. PMID: 11914346; PMCID: PMC134974.
Mardoukhi MSY, Rapp J, Irisarri I, Gunka K, Link H, Marienhagen J, de Vries J, Stulke J, Commichau FM: Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway. Microb Biotechnol. 2024 Mar;17(3):e14429. doi: 10.1111/1751-7915.14429.
Pubmed: 38483038
Cossart P, Gicquel-Sanzey B: Cloning and sequence of the crp gene of Escherichia coli K 12. Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
Pubmed: 6280141
Aiba H, Fujimoto S, Ozaki N: Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 1982 Feb 25;10(4):1345-61. doi: 10.1093/nar/10.4.1345.
Pubmed: 6280140
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
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