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Pathway Description
Quorum sensing: N-(3-oxohexanoyl)-L-homoserine lactone Biofilm Regulation
Pseudomonas aeruginosa
Category:
Metabolite Pathway
Sub-Category:
Signaling
Created: 2025-03-26
Last Updated: 2025-05-08
N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) plays a pivotal role in regulating biofilm formation through its function as a quorum-sensing signal in bacterial communication. This regulation is mediated by the activation of the LasR receptor protein by 3-oxo-C6-HSL. Once activated, LasR binds to specific promoter regions of target genes and activates the expression of key genes within the alginate biosynthetic operon (alg) and the poly-β-1,6-N-acetyl-D-glucosamine (PGA) operon. The alg operon, responsible for the synthesis of alginate, is upregulated as a response to the presence of this quorum-sensing molecule, promoting the production of alginate, which contributes to the extracellular matrix of the biofilm. Concurrently, the PGA operon facilitates the production of poly-β-1,6-N-acetyl-D-glucosamine, another crucial component of biofilm architecture. As 3-oxo-C6-HSL accumulates and activates LasR, it triggers a regulatory cascade that enhances the expression of both operons, leading to increased biofilm integrity and stability. This dual regulation not only promotes the aggregation of microbial cells within the biofilm but also enhances their resistance to environmental stresses and antimicrobial agents, underscoring the importance of quorum sensing in microbial community dynamics and biofilm formation.
References
Quorum sensing: N-(3-oxohexanoyl)-L-homoserine lactone Biofilm Regulation References
Wang, H., Wu, P., Zheng, D., Deng, L. and Wang, W., 2022. N-Acyl-homoserine lactone (AHL)-mediated microalgal–bacterial communication driving Chlorella-activated sludge bacterial biofloc formation. Environmental Science & Technology, 56(17), pp.12645-12655.
Zielinski NA, Chakrabarty AM, Berry A: Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase. J Biol Chem. 1991 May 25;266(15):9754-63.
Pubmed: 1903398
Coyne MJ Jr, Russell KS, Coyle CL, Goldberg JB: The Pseudomonas aeruginosa algC gene encodes phosphoglucomutase, required for the synthesis of a complete lipopolysaccharide core. J Bacteriol. 1994 Jun;176(12):3500-7. doi: 10.1128/jb.176.12.3500-3507.1994.
Pubmed: 7515870
Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.
Pubmed: 10984043
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0507017
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