The regulation of the epsABCDEFGHIJKLMNO operon. The operon is inhibited by sinR (HTH-type transcriptional regulator) and activated by sinI. SinR binds to the promoter inhibiting crp from binding and transcribing the operon. SinI is regulated by spo0A (Stage 0 sporulation protein A). When spo0A is present in high concentrations, sinI is expressed. Then sinI binds with sinR to form a sinI-sinR heterodimer. This caused conformational changes to sinR preventing it from binding the to promoter. Therefore, activating the expression of the operon. There are 15 products from this operon: epsA (Polysaccharide chain length determinant N-terminal domain-containing protein), epsB (non-specific protein-tyrosine kinase), epsC (Probable polysaccharide biosynthesis protein), epsD (Putative glycosyltransferase), epsE (Putative glycosyltransferase), epsF (Putative glycosyltransferase), epsG (Transmembrane protein), epsH (Putative glycosyltransferase), epsI (Putative pyruvyl transferase), epsJ (Uncharacterized glycosyltransferase), epsK (Uncharacterized membrane protein EpsK), epsL (Uncharacterized sugar transferase), epsM (UDP-N-acetylbacillosamine N-acetyltransferase), epsN (Putative pyridoxal phosphate-dependent aminotransferase), epsO (Putative pyruvyl transferase). EpsA, epsC, epsG, epsK, epsL are membrane proteins involved in biofilm formation. EpsD, epsE, epsF, epsH, epsI, epsJ, epsN and epsO are types of transferases used in the EPS production. EpsB is an enzyme used to phosphorylate tyrosine residue proteins. EpsM is an enzyme used to add an acetyl group to UDP-N-acetylbacillosamine to make UDP-N,N'-diacetylbacillosamine.

Biofilm formation: epsA, epsB, epsC, epsD, epsE, epsF, epsG, epsH, epsI, epsJ, epsK, epsL, epsM, epsN, epsO References