Browsing Pathways

Benzoate, an environmental pollutant, is utilized by bacteria such as Aromatoleum aromaticum to yield energy and carbon. While the precise transport mechanisms remain under research, it is proposed that benzoate is transported across the cell membrane passively or actively via transport proteins such as permeases and porins. Benzoate is then converted to benzoyl-CoA by benzoate-CoA ligase. Benzoyl-CoA then undergoes further degradation, catalyzed by enzymes like benzoyl-CoA reductase, leading to the formation of intermediate cyclohexane compounds which are ultimately converted into central metabolites that can enter the citrate cycle for further energy generation

Fatty acid oxidation is also known as beta-oxidation. Fatty acids are an important energy source because they are anhydrous and can be reduced. Fatty acids are good sources of energy as they yield more energy than carbohydrates. The fatty acid oxidation pathway degrades fatty acids into acetyl-CoA under anaerobic and aerobic conditions. Enzymes of this pathway can process short and long chain fatty acids. The first step in the pathway is the conversion of acyl-CoA to enoyl-CoA. The pathway continues in a cycle, each turn removing two carbon atoms from the input acyl-CoA to produce acetyl-CoA. Each turn also produces NADH.

Toluene degradation allows bacteria to use toluene, a common environmental pollutant, as both a carbon and energy source. Toluene enters the bacterial cell by passive diffusion due to its hydrophobic nature. Once within the cell, toluene undergoes a variety of enzymatic reactions. The first step is for the Gamma-Subunit of benzylsuccinate synthase to convert it into benzylsuccinate. This intermediate is then converted to Benzylsuccinyl-CoA by subunit of Benzylsuccinate CoA-transferases. Subsequently, Benzylsuccinyl-CoA undergoes a series of enzymatic reactions to form Benzoylsuccinyl-CoA, and finally benzoyl-CoA, which enters the benzoate degradation pathway, providing the bacteria with energy and carbon for growth and survival.

Toluene degradation allows bacteria to use toluene, a common environmental pollutant, as both a carbon and energy source. Toluene enters the bacterial cell by passive diffusion due to its hydrophobic nature. Once within the cell, toluene undergoes a variety of enzymatic reactions. The first step is for the Gamma-Subunit of benzylsuccinate synthase to convert it into benzylsuccinate. This intermediate is then converted to Benzylsuccinyl-CoA by subunit of Benzylsuccinate CoA-transferases. Subsequently, Benzylsuccinyl-CoA undergoes a series of enzymatic reactions to form Benzoylsuccinyl-CoA, and finally benzoyl-CoA, which enters the benzoate degradation pathway, providing the bacteria with energy and carbon for growth and survival.

Ethylbenzene degradation involves a sequence of enzymatic activities that allow bacteria to use ethylbenzene as both a carbon and energy source. Due to its hydrophobic nature, ethylbenzene can enter bacterial cells via passive diffusion across the cell membrane. Once inside, the enzyme ethylbenzene dehydrogenase activates ethylbenzene, converting it to (S)-1-phenylethanol, which is then transformed to acetophenone by (S)-1-phenylethanol dehydrogenase. Acetophenone is further converted to Benzoylacetyl-CoA, which enters the benzoate degradation route, where energy is generated and different compounds, including folate, are synthesised.

The citric acid cycle (also named tricarboxylic acid (TCA) cycle or the Krebs cycle), is a collection of 9 enzyme-catalyzed chemical reactions that occur in all living cells undergoing aerobic respiration. The citric acid cycle itself was officially identified in 1937 by Hans Adolf Krebs, who received the Nobel Prize for this discovery in 1953. In eukaryotes, the citric acid cycle occurs in the mitochondria. In prokaryotes, the TCA cycle occurs in the cytoplasm. The TCA cycle starts with acetyl-CoA, which is the “fuel” for the entire cycle. This important molecule comes from the breakdown of glycogen (a stored form of glucose), fats, and many amino acids. At beginning, acetyl-CoA first transfers its 2-carbon acetyl group to the 4-carbon acceptor compound called oxaloacetate to form the 6-carbon compound (citrate) for which the cycle is named. The resulting citrate will have numbers of chemical transformations, whereby it loses one carboxyl group (leading to the 5-carbon compound called alpha-ketoglutarate) and then a second carboxyl group (leading to the 4-carbon compound called succinate). Succinate molecule is further oxidized to fumarate, then malate and finally oxaloacetate. The regeneration of the 4-carbon oxaloacetate, allows the TCA cycle to continue. Oxidation step generates energy that is transferring energy-rich electrons for NAD+ to form NADH in TCA cycle. Each acetyl group will generate 3 NADH in TCA cycle.

Toluene degradation allows bacteria to use toluene, a common environmental pollutant, as both a carbon and energy source. Toluene enters the bacterial cell by passive diffusion due to its hydrophobic nature. Once within the cell, toluene undergoes a variety of enzymatic reactions. The first step is for the Gamma-Subunit of benzylsuccinate synthase to convert it into benzylsuccinate. This intermediate is then converted to Benzylsuccinyl-CoA by subunit of Benzylsuccinate CoA-transferases. Subsequently, Benzylsuccinyl-CoA undergoes a series of enzymatic reactions to form Benzoylsuccinyl-CoA, and finally benzoyl-CoA, which enters the benzoate degradation pathway, providing the bacteria with energy and carbon for growth and survival.

Toluene degradation allows bacteria to use toluene, a common environmental pollutant, as both a carbon and energy source. Toluene enters the bacterial cell by passive diffusion due to its hydrophobic nature. Once within the cell, toluene undergoes a variety of enzymatic reactions. The first step is for the Gamma-Subunit of benzylsuccinate synthase to convert it into benzylsuccinate. This intermediate is then converted to Benzylsuccinyl-CoA by subunit of Benzylsuccinate CoA-transferases. Subsequently, Benzylsuccinyl-CoA undergoes a series of enzymatic reactions to form Benzoylsuccinyl-CoA, and finally benzoyl-CoA, which enters the benzoate degradation pathway, providing the bacteria with energy and carbon for growth and survival.

Benzoate, an environmental pollutant, is utilized by bacteria such as Aromatoleum aromaticum to yield energy and carbon. While the precise transport mechanisms remain under research, it is proposed that benzoate is transported across the cell membrane passively or actively via transport proteins such as permeases and porins. Benzoate is then converted to benzoyl-CoA by benzoate-CoA ligase. Benzoyl-CoA then undergoes further degradation, catalyzed by enzymes like benzoyl-CoA reductase, leading to the formation of intermediate cyclohexane compounds which are ultimately converted into central metabolites that can enter the citrate cycle for further energy generation

Benzoate, an environmental pollutant, is utilized by bacteria such as Aromatoleum aromaticum to yield energy and carbon. While the precise transport mechanisms remain under research, it is proposed that benzoate is transported across the cell membrane passively or actively via transport proteins such as permeases and porins. Benzoate is then converted to benzoyl-CoA by benzoate-CoA ligase. Benzoyl-CoA is then used for phenylalanine metabolism and processes e.g., biosynthesis of alkaloids