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Pathway Description
D-Glutamine and D-Glutamate Metabolism
Pseudomonas aeruginosa
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2019-08-12
Last Updated: 2019-08-16
L-Glutamine is transported into the cytoplasm through a glutamine ABC transporter. Once inside, L-glutamine is metabolized with glutaminase to produce an L-glutamic acid. This process can be reversed through a glutamine synthetase resulting in L-glutamine. L-glutamic acid can also be transported into the cytoplasm through various methods: a glutamate/aspartate:H+ symporter GltP, a glutamate:sodium symporter, or a glutamate/aspartate ABC transporter. L-Glutamic acid can proceed to L-glutamate metabolism or it can undergo a reversible reaction through a glutamate racemase resulting in D-glutamic acid. This compound can also be obtained from D-glutamine interacting with a glutaminase. D-Glutamic acid reacts with UDP-N-acetylmuramoyl-L-alanine through an ATP-driven UDP-N-acetylmuramoylalanine-D-glutamate ligase resulting in a UDP-N-acetylmuramoyl-L-alanyl-D-glutamate which is then integrated into peptidoglycan biosynthesis. UDP-N-acetylmuramoyl-L-alanine comes from the amino sugar and nucleotide sugar metabolism product, UDP-N-acetylmuraminate which reacts with L-alanine through an ATP-driven UDP-N-acetylmuramate-L-alanine ligase.
References
D-Glutamine and D-Glutamate Metabolism References
Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M: The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes. Protein Expr Purif. 2001 Apr;21(3):393-400. doi: 10.1006/prep.2001.1390.
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Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.
Pubmed: 10984043
Lee DG, Urbach JM, Wu G, Liberati NT, Feinbaum RL, Miyata S, Diggins LT, He J, Saucier M, Deziel E, Friedman L, Li L, Grills G, Montgomery K, Kucherlapati R, Rahme LG, Ausubel FM: Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial. Genome Biol. 2006;7(10):R90. doi: 10.1186/gb-2006-7-10-r90. Epub 2006 Oct 12.
Pubmed: 17038190
El Zoeiby A, Sanschagrin F, Lamoureux J, Darveau A, Levesque RC: Cloning, over-expression and purification of Pseudomonas aeruginosa murC encoding uridine diphosphate N-acetylmuramate: L-alanine ligase. FEMS Microbiol Lett. 2000 Feb 15;183(2):281-8. doi: 10.1111/j.1574-6968.2000.tb08972.x.
Pubmed: 10675598
Nishijyo T, Park SM, Lu CD, Itoh Y, Abdelal AT: Molecular characterization and regulation of an operon encoding a system for transport of arginine and ornithine and the ArgR regulatory protein in Pseudomonas aeruginosa. J Bacteriol. 1998 Nov;180(21):5559-66.
Pubmed: 9791103
Park SM, Lu CD, Abdelal AT: Cloning and characterization of argR, a gene that participates in regulation of arginine biosynthesis and catabolism in Pseudomonas aeruginosa PAO1. J Bacteriol. 1997 Sep;179(17):5300-8. doi: 10.1128/jb.179.17.5300-5308.1997.
Pubmed: 9286980
Yan J, Deforet M, Boyle KE, Rahman R, Liang R, Okegbe C, Dietrich LEP, Qiu W, Xavier JB: Bow-tie signaling in c-di-GMP: Machine learning in a simple biochemical network. PLoS Comput Biol. 2017 Aug 2;13(8):e1005677. doi: 10.1371/journal.pcbi.1005677. eCollection 2017 Aug.
Pubmed: 28767643
Valentini M, Storelli N, Lapouge K: Identification of C(4)-dicarboxylate transport systems in Pseudomonas aeruginosa PAO1. J Bacteriol. 2011 Sep;193(17):4307-16. doi: 10.1128/JB.05074-11. Epub 2011 Jul 1.
Pubmed: 21725012
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000792
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