PathBank

Pathway Description

Asparagine Biosynthesis

Escherichia coli (strain K12 / MC4100 / BW2952)

Category:

Metabolite Pathway

Sub-Category:

Metabolic

Created: 2024-12-20

Last Updated: 2025-08-10

Asparagine is an amino acid used in protein synthesis, specifically the biosynthesis of glycoproteins. In E.coli, L-asparagine can be synthesized from L-aspartic acid by either utilizing asparagine synthetase B with L-glutamine or ammonia. Both reactions are driven by ATP however the reaction with ammonia utilizes both asparagine synthetase B and aspartate-ammonia ligase.

References

Asparagine Biosynthesis References

This pathway was propagated using PathWhiz - Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.

Propagated from SMP0000833

Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.

Visualize Compound Data

		Adenosine monophosphate
		Adenosine triphosphate
		Ammonium
		Hydrogen Ion
		L-Asparagine
		L-Aspartic acid
		L-Glutamic acid
		L-Glutamine
		Oxalacetic acid
		Oxoglutaric acid
		Pyrophosphate
		Water

Visualize Protein Data

		2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
		Glycine dehydrogenase (decarboxylating)
		Multidrug resistance protein MdtB
		Queuine tRNA-ribosyltransferase
		RNA polymerase-associated protein RapA
		Succinylornithine transaminase