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Pathway Description
Chorismate Biosynthesis
Escherichia coli APEC O1
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2024-12-25
Last Updated: 2024-12-25
Chorismate is an intermediate in tyrosine, phenylalanine and tryptophan synthesis and a precursor for folic acid, ubiquinone, enterochelin and menaquinone. Three enzymes catalyze the first step in chorismate biosynthesis. Synthesis may be reduced by feedback inhibition of tyrosine, phenylalanine and tryptophan to the enzymes. The biosynthesis of chorismate starts with D-Erythrose-4-phosphate getting transformed into 3-deoxy-D-arabino-heptulosonate-7-phosphate through a phospho-2-dehydro-3-deoxyheptonate aldolase. This is followed by a 3-dehydroquinate synthase converting the 3-deoxy-D-arabino-heptulosonate-7-phosphate into a 3-dehydroquinate which in turn is conveted to 3-dehydroshikimate through a 3-dehydroquinate dehydratase. At this point 3-dehydroshikimate can be turned into Shikimic acid through 2 different reactions involving Quinate/shikimate dehydrogenase and shikimate dehydrogenase 2. Shikimic acid is phosphorylated by Shikimate kinase 2 into shikimate 3-phosphate. Shikimate 3- phophate and a phosphoenolpyruvic acid are then joined through a 3-phosphoshikimate 1-carboxyvinyltransferase to produce a 5-enoylpyruvyl-shikimate 3-phosphate while releasing a phosphate. This in turn produces our final product Chorismate through a chorismate synthase.
References
Chorismate Biosynthesis References
Garner CC, Herrmann KM: Operator mutations of the Escherichia coli aroF gene. J Biol Chem. 1985 Mar 25;260(6):3820-5.
Pubmed: 2857723
Shultz J, Hermodson MA, Garner CC, Herrmann KM: The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. J Biol Chem. 1984 Aug 10;259(15):9655-61.
Pubmed: 6146618
Hudson GS, Davidson BE: Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12. J Mol Biol. 1984 Dec 25;180(4):1023-51. doi: 10.1016/0022-2836(84)90269-9.
Pubmed: 6396419
Hudson GS, Rellos P, Davidson BE: Two promoters control the aroH gene of Escherichia coli. Gene. 1991 Jun 15;102(1):87-91. doi: 10.1016/0378-1119(91)90544-l.
Pubmed: 1677907
Ray JM, Yanofsky C, Bauerle R: Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. J Bacteriol. 1988 Dec;170(12):5500-6. doi: 10.1128/jb.170.12.5500-5506.1988.
Pubmed: 2903857
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. doi: 10.1093/dnares/3.6.363.
Pubmed: 9097039
Davies WD, Davidson BE: The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12. Nucleic Acids Res. 1982 Jul 10;10(13):4045-58. doi: 10.1093/nar/10.13.4045.
Pubmed: 6125934
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.
Pubmed: 8905232
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Millar G, Coggins JR: The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12. FEBS Lett. 1986 May 5;200(1):11-7. doi: 10.1016/0014-5793(86)80501-4.
Pubmed: 3009224
Lyngstadaas A, Lobner-Olesen A, Boye E: Characterization of three genes in the dam-containing operon of Escherichia coli. Mol Gen Genet. 1995 Jun 10;247(5):546-54. doi: 10.1007/bf00290345.
Pubmed: 7603433
Duncan K, Chaudhuri S, Campbell MS, Coggins JR: The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase. Biochem J. 1986 Sep 1;238(2):475-83. doi: 10.1042/bj2380475.
Pubmed: 3541912
Chaudhuri S, Duncan K, Graham LD, Coggins JR: Identification of the active-site lysine residues of two biosynthetic 3-dehydroquinases. Biochem J. 1991 Apr 1;275 ( Pt 1):1-6. doi: 10.1042/bj2750001.
Pubmed: 1826831
Millar G, Lewendon A, Hunter MG, Coggins JR: The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product. Biochem J. 1986 Jul 15;237(2):427-37. doi: 10.1042/bj2370427.
Pubmed: 3026317
DeFeyter RC, Davidson BE, Pittard J: Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12. J Bacteriol. 1986 Jan;165(1):233-9. doi: 10.1128/jb.165.1.233-239.1986.
Pubmed: 3001025
DeFeyter RC, Pittard J: Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12. J Bacteriol. 1986 Jan;165(1):226-32. doi: 10.1128/jb.165.1.226-232.1986.
Pubmed: 3001024
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
Charles IG, Lamb HK, Pickard D, Dougan G, Hawkins AR: Isolation, characterization and nucleotide sequences of the aroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli. J Gen Microbiol. 1990 Feb;136(2):353-8. doi: 10.1099/00221287-136-2-353.
Pubmed: 2182772
White PJ, Millar G, Coggins JR: The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa. Biochem J. 1988 Apr 15;251(2):313-22. doi: 10.1042/bj2510313.
Pubmed: 2969724
Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. doi: 10.1093/dnares/4.2.91.
Pubmed: 9205837
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000836
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