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Pathway Description
N-Acetylneuraminate, N-Acetylmannosamine, and N-Acetylglucosamine Degradation
Escherichia coli DORA_B_14
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2025-01-30
Last Updated: 2025-01-30
The degradation of N-acetylneuraminate begins with its incorporation into the cytosol through a hydrogen symporter. Once inside the cytosol it is degraded by a N-acetylneuraminate lyase resulting in a release of a pyruvic acid and N-acetymannosamine. The latter compound is phosphorylated by an ATP driven N-Acetylmannosamine kinase resulting in the release of an ADP, a hydrogen ion and a N-Acetyl-D-mannosamine 6-phosphate. This phosphorylated compound is then metabolized by a putative N-acetylmannosamine-6-phosphate 2-epimerase resulting in the release of a N-Acetyl-D-glucosamine 6-phosphate. This compound is then deacetylated through a N-acetylglucosamine-6-phosphate deacetylase resulting in the release of an Acetic acid and a glucosamine 6-phosphate This compound can then be deaminated through a glucosamine-6-phosphate deaminase resulting in the release of an ammonium and a beta-D-fructofuranose 6-phosphate which can then be incorporated into the glycolysis pathway.
References
N-Acetylneuraminate, N-Acetylmannosamine, and N-Acetylglucosamine Degradation References
Souza JM, Plumbridge JA, Calcagno ML: N-acetylglucosamine-6-phosphate deacetylase from Escherichia coli: purification and molecular and kinetic characterization. Arch Biochem Biophys. 1997 Apr 15;340(2):338-46. doi: 10.1006/abbi.1997.9780.
Pubmed: 9143339
Ferreira FM, Mendoza-Hernandez G, Castaneda-Bueno M, Aparicio R, Fischer H, Calcagno ML, Oliva G: Structural analysis of N-acetylglucosamine-6-phosphate deacetylase apoenzyme from Escherichia coli. J Mol Biol. 2006 Jun 2;359(2):308-21. doi: 10.1016/j.jmb.2006.03.024. Epub 2006 Mar 29.
Pubmed: 16630633
Plumbridge JA: Sequence of the nagBACD operon in Escherichia coli K12 and pattern of transcription within the nag regulon. Mol Microbiol. 1989 Apr;3(4):505-15. doi: 10.1111/j.1365-2958.1989.tb00197.x.
Pubmed: 2668691
Rogers MJ, Ohgi T, Plumbridge J, Soll D: Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase. Gene. 1988;62(2):197-207. doi: 10.1016/0378-1119(88)90558-6.
Pubmed: 3284790
Altamirano MM, Plumbridge JA, Barba HA, Calcagno ML: Glucosamine-6-phosphate deaminase from Escherichia coli has a trimer of dimers structure with three intersubunit disulphides. Biochem J. 1993 Nov 1;295 ( Pt 3):645-8. doi: 10.1042/bj2950645.
Pubmed: 8240271
Peri KG, Goldie H, Waygood EB: Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli. Biochem Cell Biol. 1990 Jan;68(1):123-37.
Pubmed: 2190615
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0002044
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